MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-84
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1036 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.951e+00822 (27)67.027.51.9133.6 36689/8.4HUMANL-lactate dehydrogenase A chain (LDH-A) (LDH muscle subunit) (LDH-M)
22.037e+0058 (10)19.010.016.130.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
34.909e+0048 (10)16.010.00.11328.2 57914/8.0HUMANPyruvate kinase, M2 isozyme
44.907e+0048 (10)16.010.00.11328.2 57937/8.0HUMANPyruvate kinase, M1 isozyme (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone-binding protein) (CTHBP) (THBP1)
52.180e+0048 (10)18.010.023.649.5 58876/8.9HUMANCytochrome P450-CC24, mitochondrial precursor (P450-CC24) (Vitamin D(3) 24-hydroxylase) (1,25-dihydroxyvitamin D(3) 24-hydroxylase) (24-OHASE)
96313x.x......xxx......x.xxx.......x....xx..x.....x..xx......x...x..x..x...x........x
48753.................x.....x....x.....xx......................x........x...x........
9907.........x..........x...............x........x...x..x......xx...................
48470.........x..........x...............x........x...x..x......xx...................
53237x........x............x.........................x..............x.xx.......x.....


Detailed Results

1. 22/80 matches (27%).
Acc. #: Species: HUMAN Name: L-lactate dehydrogenase A chain (LDH-A) (LDH muscle subunit) (LDH-M)
Index: MW: 36689 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
913.5873913.58354.191990(K) (Q)
929.5732929.5824-9.91191260(K) (Y)
1118.58841118.58463.43193280(K) (E)
1119.60101119.6414-366140(K) (E)
1134.57471134.56439.23063150(K) (L)
1183.67231183.66218.61Met-ox 2692781(R)RVHPVSTMIK(G)
1246.70101246.6796173183281(K) (E)
1262.63111262.6163121581690(R) (F)
1264.65831264.6789-162332430(K) (L)
1574.93261574.915811AcetN 1141(-)ATLKDQLIYNLLK(E)
1657.86201657.85365.143570(K) (L)
1665.79621665.7729142Met-ox 60730(K)GEMMDLQHGSLFLR(T)
1757.90171757.9438-242292431(K) (L)
1890.96391890.95703.71Met-ox 58731(K)LKGEMMDLQHGSLFLR(T)
1906.00131906.0398-2082991(K) (Q)
1906.98691906.9519182Met-ox 58731(K)LKGEMMDLQHGSLFLR(T)
1944.07301944.1210-251331490(K) (I)
2036.08052036.0958-7.52Met-ox 23420(K)ITVVGVGAVGMACAISILMK(D)
2074.11962074.0821186221(K) (I)
2128.10982128.0636221Met-ox 2462650(K)GYTSWAIGLSVADLAESIMK(N)
2369.28322369.2427171Met-ox 2442651(K)LKGYTSWAIGLSVADLAESIMK(N)
2934.61702934.5651182793051(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 67% (223/332AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.61381179.6010113773860(K) (H)
1265.66731265.6378232782880(R) (K)
1475.77501475.7494172122230(K) (T)
1475.77501475.7858-7.42002110(R) (W)
1638.85961638.8604-0.491861991(K) (F)
1657.86201657.79344113290(R) (R)
1994.01661993.9772202242390(R) (R)
2150.14332150.0783302242401(R) (R)
2383.97642383.9524105195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (124/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Pyruvate kinase, M2 isozyme
Index: MW: 57914 Da pI: 8.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1118.58841118.6057-151261361(K) (G)
1246.70101246.6829141631731(K) (I)
1665.79621665.8158-122802940(R) (G)
1890.96391890.9855-113203360(R) (K)
1906.98691906.98043.41Met-ox 3203360(R)AGKPVICATQMLESMIK(K)
1922.96361922.9754-6.12Met-ox 3203360(R)AGKPVICATQMLESMIK(K)
2011.96832011.96213.01521661(K) (V)
2036.08052036.0300251872061(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (85/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Pyruvate kinase, M1 isozyme (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone-binding protein) (CTHBP) (THBP1)
Index: MW: 57937 Da pI: 8.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1118.58841118.6057-151261361(K) (G)
1246.70101246.6829141631731(K) (I)
1665.79621665.8158-122802940(R) (G)
1890.96391890.9855-113203360(R) (K)
1906.98691906.98043.41Met-ox 3203360(R)AGKPVICATQMLESMIK(K)
1922.96361922.9754-6.12Met-ox 3203360(R)AGKPVICATQMLESMIK(K)
2011.96832011.96213.01521661(K) (V)
2036.08052036.0300251872061(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (85/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Cytochrome P450-CC24, mitochondrial precursor (P450-CC24) (Vitamin D(3) 24-hydroxylase) (1,25-dihydroxyvitamin D(3) 24-hydroxylase) (24-OHASE)
Index: MW: 58876 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
913.5873913.5511401291350(R) (A)
1118.58841118.6210-293873960(R) (T)
1264.65831264.6360181791890(K) (I)
1906.00131905.9711161421571(R) (V)
2074.11962074.0762212672831(K) (A)
2096.07882096.0165302442611(K) (S)
2128.10982128.0063492Met-ox 2442611(K)TMMSTFGRMMVTPVELHK(S)
2404.25582404.1463462302511(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (93/514AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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