MS-Fit Search Results

MS-Fit Search Results

.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-85
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 514 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/67(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.316e+006	8 (11)	41.0	11.9	54.8	25.1		30773/8.6		HUMAN	Voltage-dependent anion-selective channel protein 1 (VDAC-1) (hVDAC1) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin) (Porin 31HL) (Porin 31HM)
2	5.681e+004	6 (8)	31.0	9.0	59.5	12.1		32352/8.6		MOUSE	Voltage-dependent anion-selective channel protein 1 (VDAC-1) (mVDAC1) (mVDAC5) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin)
3	1.855e+004	11 (16)	42.0	16.4	53.0	14.5		34278/8.9		HUMAN	Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor (HCDH) (Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase)
4	7886	8 (11)	16.0	11.9	48.5	39.0		87145/6.7		HUMAN	HEF-like protein
5	3423	5 (7)	19.0	7.5	19.3	83.4		40899/7.6		MOUSE	Transcription factor SOX-18

9499	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	x	.	.	.
843	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	x	.	.	.
35215	.	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	x	x
102714	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.
14111	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.

Detailed Results

1. 8/67 matches (11%).
Acc. #: Species: HUMAN Name: Voltage-dependent anion-selective channel protein 1 (VDAC-1) (hVDAC1) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin) (Porin 31HL) (Porin 31HM)
Index: MW: 30773 Da pI: 8.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1262.6793	1262.6455	27		1	12	0	(-) (S)
1374.7452	1374.6582	63		64	74	0	(R) (W)
1428.7251	1428.6469	55		225	236	0	(K) (V)
1693.9125	1693.8226	53		62	74	1	(K) (W)
1946.1123	1946.0096	53		201	218	1	(K) (F)
2103.3091	2103.1814	61		237	256	0	(K) (L)
2176.1802	2176.0522	59		75	93	0	(K) (G)
2600.3687	2600.1905	69		175	197	0	(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (117/283AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/67 matches (8%).
Acc. #: Species: MOUSE Name: Voltage-dependent anion-selective channel protein 1 (VDAC-1) (mVDAC1) (mVDAC5) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin)
Index: MW: 32352 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1374.7452 1374.6582 63 77 87 0 (R) (W)
1693.9125 1693.8226 53 75 87 1 (K) (W)
1946.1123 1946.0096 53 214 231 1 (K) (F)
2103.3091 2103.1814 61 250 269 0 (K) (L)
2176.1802 2176.0522 59 88 106 0 (K) (G)
2600.3687 2600.1905 69 188 210 0 (K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (93/296AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/67 matches (16%).
Acc. #: Species: HUMAN Name: Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor (HCDH) (Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase)
Index: MW: 34278 Da pI: 8.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
931.5631 931.5213 45 69 76 1 (K) (V)
931.5631 931.5213 45 68 75 1 (K) (K)
1018.5833 1018.5322 50 213 221 0 (K) (L)
1172.6377 1172.5839 46 193 202 0 (K) (A)
1317.8237 1317.7605 48 222 232 0 (R) (L)
1333.8250 1333.7554 52 1Met-ox 222 232 0 (R)LLVPYLMEAIR(L)
1619.9268 1619.8408 53 1Met-ox 166 179 0 (R)FAGLHFFNPVPVMK(L)
1740.9848 1740.8855 57 207 221 1 (K) (L)
2019.1470 2019.0275 59 1Met-ox 163 179 1 (R)QDRFAGLHFFNPVPVMK(L)
2373.3280 2373.1688 67 1Met-ox 250 271 0 (K)LGAGYPMGPFELLDYVGLDTTK(F)
2752.5301 2752.3906 51 137 162 0 (K) (Q)
2777.4752 2777.2993 63 1Met-ox 272 295 0 (K)FIVDGWHEMDAENPLHQPSPSLNK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (134/314AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/67 matches (11%).
Acc. #: Species: HUMAN Name: HEF-like protein
Index: MW: 87145 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1172.6377 1172.5548 71 482 491 0 (R) (E)
1349.8265 1349.7503 56 142 152 1 (R) (Q)
1599.9046 1599.8165 55 1Met-ox 289 302 0 (R)SLTPQLNNNVPMQK(K)
1740.9848 1740.8669 68 611 624 1 (K) (S)
1740.9848 1740.9431 24 731 745 1 (R) (D)
1827.0624 1826.9547 59 287 302 1 (R) (K)
2009.0697 2009.0456 12 164 181 0 (R) (G)
2110.2508 2110.1483 49 68 85 0 (R) (G)
2529.4868 2529.3789 43 203 227 1 (K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (127/786AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/67 matches (7%).
Acc. #: Species: MOUSE Name: Transcription factor SOX-18
Index: MW: 40899 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1619.9268 1619.8667 37 79 91 1 (R) (D)
1635.9465 1635.8616 52 1Met-ox 79 91 1 (R)IRRPMNAFMVWAK(D)
1647.9786 1647.8818 59 97 111 0 (R) (M)
2011.9863 2012.0340 -24 193 211 0 (R) (S)
2716.3933 2716.4674 -27 162 189 0 (R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (75/377AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.