MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-86
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1697 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/94(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.382e+01017 (18)71.018.114.930.0 30773/8.6HUMANVoltage-dependent anion-selective channel protein 1 (VDAC-1) (hVDAC1) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin) (Porin 31HL) (Porin 31HM)
26.076e+00914 (14)53.014.913.728.5 32352/8.6MOUSEVoltage-dependent anion-selective channel protein 1 (VDAC-1) (mVDAC1) (mVDAC5) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin)
37.009e+00514 (14)18.014.94.9658.1 95315/6.4MOUSEElongation factor 2 (EF-2)
43.719e+00513 (13)17.013.83.1358.8 95339/6.4HUMANElongation factor 2 (EF-2)
51.853e+0059 (9)16.09.64.0550.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
9120.x.......x.......x....x..............x.....xx...x....x...............x...xx..........x...xxx.x
819.x.......x............x..............x.....x....x....x...............x...xx..........x...xx..x
39136..xxx.......................x..x.............x...x.x............x...x......xxx...............x
57575..xxx.......................x..x.................x.x............x...x......xxx...............x
46726....x..............x......xx.......x...x.........x......x....x................................


Detailed Results

1. 17/94 matches (18%).
Acc. #: Species: HUMAN Name: Voltage-dependent anion-selective channel protein 1 (VDAC-1) (hVDAC1) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin) (Porin 31HL) (Porin 31HM)
Index: MW: 30773 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
947.5059947.5202-152752830(K) (-)
1030.61171030.6148-3.12572660(K) (N)
1173.62151173.61565.0AcetN 1120(-)AVPPTYADLGK(S)
1213.63121213.62177.81641740(R) (T)
1374.67051374.65829.064740(R) (W)
1400.69131400.669815971090(K) (K)
1428.66471428.6469122252360(K) (V)
1456.75721456.75491.61621741(K) (T)
1528.79141528.764717971101(K) (N)
1817.93671817.9146122022180(K) (F)
1946.04121946.0096162012181(K) (F)
1959.91741959.87832035530(K) (V)
2103.23222103.1814242372560(K) (L)
2176.10402176.05222475930(K) (G)
2189.05652189.02101633531(K) (V)
2190.09222189.9960441Met-ox 1211390(R)EHINLGCDMDFDIAGPSIR(G)
2600.31002600.1905461751970(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 71% (201/283AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/94 matches (14%).
Acc. #: Species: MOUSE Name: Voltage-dependent anion-selective channel protein 1 (VDAC-1) (mVDAC1) (mVDAC5) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin)
Index: MW: 32352 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
947.5059947.5202-152882960(K) (-)
1030.61171030.6148-3.12702790(K) (N)
1213.63121213.62177.81771870(R) (T)
1374.67051374.65829.077870(R) (W)
1400.69131400.6698151101220(K) (K)
1456.75721456.75491.61751871(K) (T)
1528.79141528.7647171101231(K) (N)
1817.93671817.9146122152310(K) (F)
1946.04121946.0096162142311(K) (F)
1959.91741959.87832048660(K) (V)
2103.23222103.1814242502690(K) (L)
2176.10402176.052224881060(K) (G)
2189.05652189.02101646661(K) (V)
2600.31002600.1905461882100(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (158/296AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/94 matches (14%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
961.5127961.50428.85996051(K) (A)
969.5100969.5482-397177260(R) (R)
973.5087973.50088.24084151(K) (V)
1307.70621307.65304121320(R) (S)
1323.64651323.6479-1.11Met-ox 21320(R)NMSVIAHVDHGK(S)
1434.77541434.7341292362491(K) (A)
1475.78421475.7901-4.01511621(R) (A)
1486.78971486.740433pyroGlu 2272391(K)QFAEMYVAKFAAK(G)
1707.79861707.8740-44AcetN 1Met-ox 1151(-)VNFTVDQIRAIMDK(K)
1799.91431799.89689.77868010(K) (S)
1962.02351961.9795227687850(R) (A)
1978.00901977.9744171Met-ox 7687850(R)GHVFEESQVAGTPMFVVK(A)
1990.03151989.9639341Met-ox 4824981(K)TGTITTFEHAHNMRVMK(F)
2600.31002600.4274-453433660(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (162/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/94 matches (13%).
Acc. #: Species: HUMAN Name: Elongation factor 2 (EF-2)
Index: MW: 95339 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
961.5127961.50428.85996051(K) (A)
969.5100969.5482-397177260(R) (R)
973.5087973.50088.24084151(K) (V)
1307.70621307.65304121320(R) (S)
1323.64651323.6479-1.11Met-ox 21320(R)NMSVIAHVDHGK(S)
1475.78421475.7901-4.01511621(R) (A)
1486.78971486.740433pyroGlu 2272391(K)QFAEMYVAKFAAK(G)
1707.79861707.8740-44AcetN 1Met-ox 1151(-)VNFTVDQIRAIMDK(K)
1799.91431799.89689.77868010(K) (S)
1962.02351961.9795227687850(R) (A)
1978.00901977.9744171Met-ox 7687850(R)GHVFEESQVAGTPMFVVK(A)
1990.03151989.9639341Met-ox 4824981(K)TGTITTFEHAHNMRVMK(F)
2600.31002600.4274-453433660(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (152/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/94 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.5087973.5318-243964030(K) (V)
1179.63061179.6010253773860(K) (H)
1277.70081277.7105-7.64734830(K) (T)
1300.59131300.5302472582670(K) (N)
1357.64071357.6963-414444550(K) (E)
1383.70741383.6909121861970(K) (V)
1475.78421475.7494242122230(K) (T)
1475.78421475.7858-1.12002110(R) (W)
1599.84781599.83428.54424551(K) (E)
1657.78981657.7934-2.213290(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (106/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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