MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-89
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 218 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
18.510e+00614 (23)56.023.3-26.023.8 28804/6.7HUMANPhosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
28.502e+00614 (23)56.023.3-26.023.8 28832/6.7MOUSEPhosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
31.316e+0047 (11)21.011.7-15.341.1 52004/6.1MOUSEAntithrombin-III precursor (ATIII)
481595 (8)8.08.3-16.843.7 69628/5.3MOUSERING finger protein 17
532656 (10)6.010.0-8.6730.5 94506/5.6HUMANOsmotic stress protein 94 (Heat shock 70-related protein APG-1)
115473..xx.x.x.....x..........xx...x........xx.......x......xx..x.
77310..xx.x.x.....x..........xx...x........xx.......x......xx..x.
60362......x.x.......xx..................x..x........x...........
11830......x.x..................xx....................x..........
72178.........xxx............x..............x..x.................



Detailed Results

1. 14/60 matches (23%).
Acc. #: Species: HUMAN Name: Phosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
Index: MW: 28804 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1059.50911059.5587-47911000(R) (A)
1150.62561150.6697-381811910(R) (G)
1306.71391306.7708-431801911(K) (G)
1312.56211312.6034-3111210(R) (F)
1683.86371683.9110-281631760(R) (E)
1793.75271793.8056-2947610(R) (R)
1882.82591882.8744-261421570(R) (D)
1979.82841979.8775-2522390(R) (R)
2131.08492131.1221-181Met-ox 2232400(K)NLKPIKPMQFLGDEETVR(K)
2135.94402135.9787-1622401(R) (G)
2259.15742259.2171-261Met-ox 2232411(K)NLKPIKPMQFLGDEETVRK(A)
2433.09092433.1073-6.71Met-ox 1181380(R)SYDVPPPPMEPDHPFYSNISK(D)
2439.12052439.1713-211421621(R) (A)
2589.18842589.2084-7.71Met-ox 1171381(R)RSYDVPPPPMEPDHPFYSNISK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 56% (143/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/60 matches (23%).
Acc. #: Species: MOUSE Name: Phosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
Index: MW: 28832 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1059.50911059.5587-47911000(R) (A)
1150.62561150.6697-381811910(R) (G)
1306.71391306.7708-431801911(K) (G)
1312.56211312.6034-3111210(R) (F)
1683.86371683.9110-281631760(R) (E)
1793.75271793.8056-2947610(R) (R)
1882.82591882.8744-261421570(R) (D)
1979.82841979.8775-2522390(R) (R)
2131.08492131.1221-181Met-ox 2232400(K)NLKPIKPMQFLGDEETVR(K)
2135.94402135.9787-1622401(R) (G)
2259.15742259.2171-261Met-ox 2232411(K)NLKPIKPMQFLGDEETVRK(A)
2433.09092433.1073-6.71Met-ox 1181380(R)SYDVPPPPMEPDHPFYSNISK(D)
2439.12052439.1713-211421621(R) (A)
2589.18842589.2084-7.71Met-ox 1171381(R)RSYDVPPPPMEPDHPFYSNISK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 56% (143/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: Antithrombin-III precursor (ATIII)
Index: MW: 52004 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1310.55181310.5799-211140(-) (K)
1326.58931326.5748111Met-ox 1140(-)MYSPGAGSGAAGER(K)
1697.76871697.8460-46pyroGlu 1341471(K)QLMEVFKFDTISEK(T)
1700.85551700.8608-3.12032161(K) (V)
2114.98222114.97135.12752921(K) (Y)
2135.94402136.0137-331842020(K) (L)
2299.08162299.1280-201041240(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (100/465AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: RING finger protein 17
Index: MW: 69628 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1310.55181310.5721-151041140(K) (T)
1326.58931326.5670171Met-ox 1041140(K)MVNNCSSDLEK(T)
1962.86761962.9265-301190(-) (S)
1978.83971978.9214-411Met-ox 1190(-)MAAEASSTGLASCHLVESK(S)
2334.11332334.1465-143423630(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (52/626AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Osmotic stress protein 94 (Heat shock 70-related protein APG-1)
Index: MW: 94506 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1328.56531328.5905-195295390(K) (C)
1344.55191344.5854-251Met-ox 5295390(K)MQVDQEEGHQK(C)
1475.70471475.7342-203613740(K) (G)
1793.75271793.7652-7.01Met-ox 7237361(K)DERYDHLDPTEMEK(V)
2135.94402135.9192125015190(K) (N)
2151.92962151.91417.21Met-ox 5015190(K)QNLEGDHSDAPMETETSFK(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (58/839AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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