MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-90
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 394 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/53(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	4.928e+005	8 (15)	40.0	15.1	4.52	41.4		26670/6.4		HUMAN	Triosephosphate isomerase (TIM)
2	2.477e+004	7 (13)	39.0	13.2	10.3	38.3		25712/6.2		HUMAN	Glutathione S-transferase Mu 1 (GSTM1-1) (HB subunit 4) (GTH4) (GSTM1a-1a) (GSTM1b-1b) (GST class-mu 1)
3	1763	5 (9)	22.0	9.4	-6.32	26.3		26713/6.9		MOUSE	TRIOSEPHOSPHATE ISOMERASE (TIM)
4	1545	6 (11)	9.0	11.3	14.6	39.6		66361/8.3		HUMAN	Parathyroid hormone/parathyroid hormone-related peptide receptor precursor (PTH/PTHR receptor)
5	1331	6 (11)	15.0	11.3	-12.4	73.2		44954/6.3		MOUSE	GPI-anchor transamidase (GPI transamidase) (Phosphatidylinositol-glycan biosynthesis, class K protein) (PIG-K)

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23594	.	.	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
65512	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.

Detailed Results

1. 8/53 matches (15%).
Acc. #: Species: HUMAN Name: Triosephosphate isomerase (TIM)
Index: MW: 26670 Da pI: 6.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
954.4747	954.4838	-9.5		7	14	0	(K) (M)
1082.5718	1082.5787	-6.4		6	14	1	(R) (M)
1234.6368	1234.6028	28		195	206	0	(K) (I)
1458.7266	1458.7229	2.6		101	113	0	(R) (V)
1600.7898	1600.7542	22		86	99	0	(K) (R)
1602.8475	1602.8896	-26		161	175	0	(K) (T)
1821.9781	1821.9897	-6.3		114	131	0	(K) (L)
2206.1566	2206.0854	32		34	53	0	(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (101/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/53 matches (13%).
Acc. #: Species: HUMAN Name: Glutathione S-transferase Mu 1 (GSTM1-1) (HB subunit 4) (GTH4) (GSTM1a-1a) (GSTM1b-1b) (GST class-mu 1)
Index: MW: 25712 Da pI: 6.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
975.5063 975.5165 -10 145 152 0 (K) (I)
1090.6188 1090.6413 -21 203 211 0 (R) (M)
1487.7175 1487.6800 25 83 94 1 (R) (I)
1536.8584 1536.8208 24 70 82 0 (K) (K)
1589.7887 1589.7587 19 19 31 0 (R) (K)
1709.8807 1709.8685 7.1 174 187 1 (K) (F)
1883.0589 1883.0067 28 53 69 0 (K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (86/218AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/53 matches (9%).
Acc. #: Species: MOUSE Name: TRIOSEPHOSPHATE ISOMERASE (TIM)
Index: MW: 26713 Da pI: 6.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.4747 954.4838 -9.5 7 14 0 (K) (M)
1082.5718 1082.5787 -6.4 6 14 1 (R) (M)
1458.7266 1458.7229 2.6 101 113 0 (R) (V)
1602.8475 1602.8896 -26 161 175 0 (K) (T)
1837.9993 1837.9846 8.0 114 131 0 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (55/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/53 matches (11%).
Acc. #: Species: HUMAN Name: Parathyroid hormone/parathyroid hormone-related peptide receptor precursor (PTH/PTHR receptor)
Index: MW: 66361 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1036.5189 1036.5176 1.3 96 104 1 (K) (G)
1165.5779 1165.5966 -16 173 181 1 (K) (E)
1234.6368 1234.6180 15 68 79 0 (K) (K)
1602.8475 1602.8161 20 54 67 0 (K) (G)
1821.9781 1821.9297 27 220 233 0 (R) (A)
1837.9993 1837.9246 41 1Met-ox 220 233 0 (R)NYIHMHLFLSFMLR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (58/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/53 matches (11%).
Acc. #: Species: MOUSE Name: GPI-anchor transamidase (GPI transamidase) (Phosphatidylinositol-glycan biosynthesis, class K protein) (PIG-K)
Index: MW: 44954 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
947.5059 947.5413 -37 309 316 1 (R) (I)
1495.7683 1495.8273 -39 297 309 1 (K) (V)
1707.8256 1707.9070 -48 294 308 1 (R) (K)
1821.9781 1821.9322 25 155 171 0 (K) (F)
1837.9993 1837.9271 39 1Met-ox 155 171 0 (K)SNILIYMTGHGGNGFLK(F)
2225.1977 2225.2294 -14 126 145 1 (R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (60/395AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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