MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-92
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 419 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/58(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	4.280e+006	11 (18)	56.0	19.0	-8.04	46.7		26670/6.4		HUMAN	Triosephosphate isomerase (TIM)
2	2133	7 (12)	30.0	12.1	-15.2	51.5		26713/6.9		MOUSE	TRIOSEPHOSPHATE ISOMERASE (TIM)
3	772	5 (8)	11.0	8.6	4.10	48.9		35966/9.1		HUMAN	Voltage-dependent calcium channel gamma-2 subunit (Neuronal voltage-gated calcium channel gamma-2 subunit)
4	550	7 (12)	8.0	12.1	13.8	41.3		89228/7.2		HUMAN	Semaphorin 3E precursor
5	498	6 (10)	9.0	10.3	0.875	56.7		83201/5.3		MOUSE	Myosin Vb (Myosin 5B)

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Detailed Results

1. 11/58 matches (18%).
Acc. #: Species: HUMAN Name: Triosephosphate isomerase (TIM)
Index: MW: 26670 Da pI: 6.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
954.4573	954.4838	-28		7	14	0	(K) (M)
1082.5517	1082.5787	-25		6	14	1	(R) (M)
1151.5517	1151.5883	-32		60	69	0	(K) (V)
1234.5877	1234.6028	-12		195	206	0	(K) (I)
1458.7114	1458.7229	-7.9		101	113	0	(R) (V)
1600.7642	1600.7542	6.3		86	99	0	(K) (R)
1602.8205	1602.8896	-43		161	175	0	(K) (T)
1614.8146	1614.8240	-5.8		100	113	1	(R) (V)
1821.9988	1821.9897	5.0		114	131	0	(K) (L)
2206.1269	2206.0854	19		34	53	0	(K) (Q)
3029.6897	3029.5835	35		220	248	0	(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 56% (141/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/58 matches (12%).
Acc. #: Species: MOUSE Name: TRIOSEPHOSPHATE ISOMERASE (TIM)
Index: MW: 26713 Da pI: 6.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.4573 954.4838 -28 7 14 0 (K) (M)
1082.5517 1082.5787 -25 6 14 1 (R) (M)
1151.5517 1151.5883 -32 60 69 0 (K) (V)
1458.7114 1458.7229 -7.9 101 113 0 (R) (V)
1602.8205 1602.8896 -43 161 175 0 (K) (T)
1614.8146 1614.8240 -5.8 100 113 1 (R) (V)
3029.6897 3029.5835 35 220 248 0 (K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (77/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/58 matches (8%).
Acc. #: Species: HUMAN Name: Voltage-dependent calcium channel gamma-2 subunit (Neuronal voltage-gated calcium channel gamma-2 subunit)
Index: MW: 35966 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1114.4998 1114.5241 -22 309 318 0 (K) (R)
1458.7114 1458.6800 22 54 65 0 (K) (T)
1586.7419 1586.7749 -21 53 65 1 (K) (T)
1602.8205 1602.7698 32 1Met-ox 53 65 1 (K)KNEEVMTHSGLWR(T)
1616.8016 1616.7855 10.0 293 305 0 (R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (36/323AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/58 matches (12%).
Acc. #: Species: HUMAN Name: Semaphorin 3E precursor
Index: MW: 89228 Da pI: 7.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
901.3728 901.3652 8.5 214 220 0 (R) (L)
954.4573 954.4099 50 102 108 0 (K) (G)
978.4804 978.4831 -2.8 732 738 1 (K) (R)
1037.5067 1037.5056 1.0 547 555 0 (R) (R)
1155.5574 1155.5212 31 1Met-ox 102 110 1 (K)MEECIMKGK(D)
1614.8146 1614.8280 -8.3 712 724 0 (K) (V)
2220.1944 2220.1566 17 319 337 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (64/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/58 matches (10%).
Acc. #: Species: MOUSE Name: Myosin Vb (Myosin 5B)
Index: MW: 83201 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
948.4313 948.4427 -12 202 209 0 (R) (H)
976.4967 976.4587 39 116 123 0 (R) (K)
1076.5070 1076.5159 -8.2 179 187 0 (R) (R)
1495.7399 1495.8017 -41 53 65 1 (K) (R)
1553.7582 1553.7205 24 576 588 1 (R) (Y)
1614.8146 1614.8087 3.6 321 335 0 (K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (66/723AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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