MS-Fit Search Results


.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-93
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 428 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/70(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.535e+00515 (21)48.021.4-10.926.1 29471/7.6MOUSEProteasome subunit alpha type 4 (Proteasome component C9) (Macropain subunit C9) (Multicatalytic endopeptidase complex subunit C9) (Proteasome subunit L)
21.534e+00515 (21)48.021.4-10.926.1 29484/7.6HUMANProteasome subunit alpha type 4 (Proteasome component C9) (Macropain subunit C9) (Multicatalytic endopeptidase complex subunit C9) (Proteasome subunit L)
339306 (8)21.08.69.4341.6 40841/9.4MOUSERNA 3'-terminal phosphate cyclase-like protein
427066 (8)12.08.6-21.154.5 73095/7.0HUMANDEAD-box protein 3, Y-chromosomal
526546 (8)12.08.67.5169.8 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
6359x.......x.......x..x.x..........xx...x..x.x.x..xxx...................x
4842x.......x.......x..x.x..........xx...x..x.x.x..xxx...................x
27972...........................x.......................x.x.x....xx........
29012...........x......x.......x..........................x...x.......x....
49976....x...............................x....................x.xxx........


Detailed Results

1. 15/70 matches (21%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 4 (Proteasome component C9) (Macropain subunit C9) (Multicatalytic endopeptidase complex subunit C9) (Proteasome subunit L)
Index: MW: 29471 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
858.4768858.4937-202322380(K) (K)
902.4983902.5311-362112180(K) (E)
982.4434982.4634-20pyroGlu 1191270(K)QAYTQFGGK(R)
999.4797999.47475.02392461(K) (A)
999.4797999.4900-101191270(K) (R)
1007.50531007.5162-119170(R) (L)
1097.59771097.6206-21pyroGlu 2302381(K)QKEVEQLIK(K)
1114.63581114.6472-102302381(K) (K)
1226.61331226.6309-1455640(K) (I)
1340.56701340.6044-28pyroGlu 1Met-ox 1771871(K)QDYKEGEMTLK(S)
1357.61941357.6310-8.51Met-ox 1771871(K)QDYKEGEMTLK(S)
1430.81471430.8219-5.02062181(K) (E)
1637.79661637.7991-1.51Met-ox 1611760(K)ATCIGNNSAAAVSMLK(Q)
1650.87801650.9008-141281410(K) (H)
1718.92141718.91175.651641(R) (I)
2623.27382623.2344151Met-ox 68910(K)LNEDMACSVAGITSDANVLTNELR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (127/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/70 matches (21%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 4 (Proteasome component C9) (Macropain subunit C9) (Multicatalytic endopeptidase complex subunit C9) (Proteasome subunit L)
Index: MW: 29484 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
858.4768858.4937-202322380(K) (K)
902.4983902.5311-362112180(K) (E)
982.4434982.4634-20pyroGlu 1191270(K)QAYTQFGGK(R)
999.4797999.47475.02392461(K) (A)
999.4797999.4900-101191270(K) (R)
1007.50531007.5162-119170(R) (L)
1097.59771097.6206-21pyroGlu 2302381(K)QKEVEQLIK(K)
1114.63581114.6472-102302381(K) (K)
1226.61331226.6309-1455640(K) (I)
1340.56701340.6044-28pyroGlu 1Met-ox 1771871(K)QDYKEGEMTLK(S)
1357.61941357.6310-8.51Met-ox 1771871(K)QDYKEGEMTLK(S)
1430.81471430.8219-5.02062181(K) (E)
1637.79661637.7991-1.51Met-ox 1611760(K)ATCIGNNSAAAVSMLK(Q)
1650.87801650.9008-141281410(K) (H)
1718.92141718.91175.651641(R) (I)
2623.27382623.2344151Met-ox 68910(K)LNEDMACSVAGITSDANVLTNELR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (127/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/70 matches (8%).
Acc. #: Species: MOUSE Name: RNA 3'-terminal phosphate cyclase-like protein
Index: MW: 40841 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.52071033.51812.53333400(K) (V)
1859.00231858.9308381581751(R) (V)
1940.93691940.9111131170(-) (Q)
1997.00991997.0200-5.13523701(K) (T)
2211.11162211.1524-182142311(R) (G)
2225.12832225.0708261191(-) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (82/373AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/70 matches (8%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3, Y-chromosomal
Index: MW: 73095 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
912.3513912.3811-336216300(R) (G)
989.3866989.4328-4767750(K) (D)
1029.46521029.5118-454794860(R) (S)
1940.93691940.9792-223343491(K) (M)
2083.01542083.0249-4.65105260(R) (I)
2518.21872518.1574245635831(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (81/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/70 matches (8%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
880.3427880.3657-262252300(R) (K)
1136.57171136.5192461982061(R) (A)
2083.01542083.0936-382072241(R) (C)
2195.14452195.13952.34284451(R) (C)
2211.11162211.0756161431610(K) (T)
2225.12832225.0304446066240(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (89/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.