MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-94
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 813 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/80(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	4.208e+005	12 (15)	51.0	15.0	12.9	27.7		27844/8.2		HUMAN	Electron transfer flavoprotein beta-subunit (Beta-ETF)
2	4.366e+004	9 (11)	38.0	11.3	18.5	14.9		26478/7.7		HUMAN	Adenylate kinase isoenzyme 2, mitochondrial (ATP-AMP transphosphorylase)
3	9779	7 (8)	18.0	8.8	-14.7	48.9		70638/7.6		HUMAN	Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP] (Phosphoenolpyruvate carboxylase) (PEPCK-M)
4	6652	7 (8)	14.0	8.8	2.20	51.4		76724/6.3		MOUSE	Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Hexosephosphate aminotransferase 1) (D-fructose-6-phosphate amidotransferase 1) (GFAT 1) (GFAT1)
5	5245	7 (8)	11.0	8.8	8.16	60.0		90003/5.7		MOUSE	Signal transducer and activator of transcription 5B

22608	x	.	.	x	x	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
104849	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	x	.	.	.
19130	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.
3110	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
51809	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.

Detailed Results

1. 12/80 matches (15%).
Acc. #: Species: HUMAN Name: Electron transfer flavoprotein beta-subunit (Beta-ETF)
Index: MW: 27844 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
853.5432	853.5260	20		99	106	0	(R) (V)
875.4712	875.4838	-14		27	35	0	(R) (H)
921.4574	921.4464	12	1Met-ox	77	85	0	(R)TALAMGADR(G)
1054.6351	1054.6261	8.6		177	186	0	(K) (L)
1066.5458	1066.5607	-14	1Met-ox	192	200	0	(R)YATLPNIMK(A)
1102.5947	1102.5744	18		165	174	0	(R) (L)
1295.8337	1295.8051	22		175	186	1	(R) (L)
1339.7534	1339.7222	23		222	233	0	(K) (T)
1403.7608	1403.7283	23		86	98	0	(R) (L)
1683.9736	1683.9533	12		206	221	0	(K) (L)
1918.9334	1918.8791	28	1Met-ox	36	51	0	(K)HSMNPFCEIAVEEAVR(L)
1945.9760	1945.9476	15		60	76	0	(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 51% (131/255AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: Adenylate kinase isoenzyme 2, mitochondrial (ATP-AMP transphosphorylase)
Index: MW: 26478 Da pI: 7.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
979.6003 979.5940 6.3 18 28 0 (R) (G)
1135.6080 1135.5900 16 94 103 0 (K) (T)
1519.8178 1519.7929 16 1Met-ox 73 85 0 (K)LVSDEMVVELIEK(N)
1589.9386 1589.9127 16 18 34 1 (R) (L)
1817.0275 1817.0060 12 122 137 0 (K) (R)
1997.0545 1997.0133 21 187 202 0 (R) (K)
2051.0148 2050.9513 31 1Met-ox 35 51 0 (R)LAENFCVCHLATGDMLR(A)
2074.1879 2074.1436 21 120 137 1 (K) (R)
2266.2590 2266.1984 27 185 202 1 (K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (93/239AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP] (Phosphoenolpyruvate carboxylase) (PEPCK-M)
Index: MW: 70638 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1135.6080 1135.6224 -13 235 244 0 (K) (E)
1425.7414 1425.8066 -46 32 45 0 (R) (D)
1826.9101 1826.9652 -30 245 262 1 (R) (C)
1918.9334 1918.9874 -28 609 624 1 (R) (E)
1938.9526 1938.9822 -15 156 173 0 (R) (I)
2211.1608 2211.1630 -1.00 174 193 1 (R) (L)
2266.2590 2266.1906 30 275 295 1 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (117/640AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/80 matches (8%).
Acc. #: Species: MOUSE Name: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Hexosephosphate aminotransferase 1) (D-fructose-6-phosphate amidotransferase 1) (GFAT 1) (GFAT1)
Index: MW: 76724 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
875.4712 875.4521 22 362 367 1 (K) (R)
1102.5947 1102.5897 4.5 530 538 0 (K) (S)
1817.0275 1816.9744 29 369 385 0 (R) (Q)
1826.9101 1826.8687 23 96 111 0 (R) (S)
1888.9968 1888.9921 2.5 115 130 0 (K) (D)
1917.9700 1918.0358 -34 438 456 1 (K) (E)
1931.9675 1932.0273 -31 586 601 1 (K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (99/681AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/80 matches (8%).
Acc. #: Species: MOUSE Name: Signal transducer and activator of transcription 5B
Index: MW: 90003 Da pI: 5.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1136.6008 1136.5700 27 218 226 0 (R) (V)
1179.5802 1179.6122 -27 650 659 1 (R) (L)
1683.9736 1683.9070 40 72 86 1 (K) (L)
1840.9533 1840.9670 -7.5 182 197 0 (R) (M)
1945.9760 1946.0401 -33 584 600 0 (K) (Q)
2074.1879 2074.1350 25 583 600 1 (K) (Q)
2681.4519 2681.3649 32 682 705 1 (K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (92/786AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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