MS-Fit Search Results

MS-Fit Search Results

.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-95
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 256 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/79(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5.749e+006	15 (18)	68.0	19.0	2.41	65.1		27887/8.6		HUMAN	Proteasome subunit alpha type 7 (Proteasome subunit RC6-1) (Proteasome subunit XAPC7)
2	3.801e+006	14 (17)	63.0	17.7	4.04	66.3		27855/8.6		MOUSE	Proteasome subunit alpha type 7 (Proteasome subunit RC6-1)
3	2.589e+004	6 (7)	26.0	7.6	15.3	54.0		27866/8.8		MOUSE	Proteasome subunit alpha type 7-like
4	2.526e+004	6 (7)	25.0	7.6	15.3	54.0		28558/9.1		HUMAN	Proteasome subunit alpha type 7-like
5	1.260e+004	4 (5)	13.0	5.1	4.99	51.1		32982/4.8		HUMAN	Metaphase chromosomal protein 1 (33 kDa metaphase chromosomal protein)

77094	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	x	x	.	.
27727	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	x	x	.	.
13470	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.
8502	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.
14847	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	x	.	.	.	.	.	.	.	.	.

Detailed Results

1. 15/79 matches (18%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 7 (Proteasome subunit RC6-1) (Proteasome subunit XAPC7)
Index: MW: 27887 Da pI: 8.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
978.5342	978.5736	-40		110	117	1	(R) (Y)
1007.5237	1007.5526	-29		167	174	1	(K) (N)
1045.5282	1045.5642	-34		49	57	1	(K) (T)
1117.5687	1117.6152	-42	1Met-ox	205	213	1	(K)NIELAVMRR(D)
1156.5180	1156.5645	-40		87	95	1	(R) (L)
1184.6761	1184.7003	-20		37	47	1	(R) (K)
1634.8840	1634.8641	12		96	109	0	(R) (Y)
1666.8056	1666.7865	11		144	157	0	(R) (A)
2043.1678	2043.1272	20	1Met-ox	194	212	1	(K)ALLEVVQSGGKNIELAVMR(R)
2065.1288	2065.0871	20		125	143	0	(R) (L)
2249.1736	2249.1103	28		144	163	1	(R) (G)
2356.2024	2356.1099	39	1Met-ox	61	81	1	(R)KICALDDNVCMAFAGLTADAR(I)
2449.3256	2449.2404	35		6	27	0	(R) (K)
2577.4383	2577.3353	40		6	28	1	(R) (G)
2871.5578	2871.4542	36		118	143	1	(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 68% (169/248AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/79 matches (17%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 7 (Proteasome subunit RC6-1)
Index: MW: 27855 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
978.5342 978.5736 -40 110 117 1 (R) (Y)
1007.5237 1007.5526 -29 167 174 1 (K) (N)
1045.5282 1045.5642 -34 49 57 1 (K) (T)
1117.5687 1117.6152 -42 1Met-ox 205 213 1 (K)NIELAVMRR(D)
1156.5180 1156.5645 -40 87 95 1 (R) (L)
1634.8840 1634.8641 12 96 109 0 (R) (Y)
1666.8056 1666.7865 11 144 157 0 (R) (A)
2043.1678 2043.1272 20 1Met-ox 194 212 1 (K)ALLEVVQSGGKNIELAVMR(R)
2065.1288 2065.0871 20 125 143 0 (R) (L)
2249.1736 2249.1103 28 144 163 1 (R) (G)
2356.2024 2356.1099 39 1Met-ox 61 81 1 (R)KICALDDNVCMAFAGLTADAR(I)
2449.3256 2449.2404 35 6 27 0 (R) (K)
2577.4383 2577.3353 40 6 28 1 (R) (G)
2871.5578 2871.4542 36 118 143 1 (R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 63% (158/248AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/79 matches (7%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 7-like
Index: MW: 27866 Da pI: 8.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1045.5282 1045.5642 -34 51 59 1 (K) (T)
1634.8840 1634.8641 12 98 111 0 (K) (F)
1666.8056 1666.7865 11 146 159 0 (R) (A)
2249.1736 2249.1103 28 146 165 1 (R) (S)
2449.3256 2449.2404 35 8 29 0 (R) (K)
2577.4383 2577.3353 40 8 30 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (66/250AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/79 matches (7%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 7-like
Index: MW: 28558 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1045.5282 1045.5642 -34 51 59 1 (K) (T)
1634.8840 1634.8641 12 104 117 0 (K) (F)
1666.8056 1666.7865 11 152 165 0 (R) (A)
2249.1736 2249.1103 28 152 171 1 (R) (S)
2449.3256 2449.2404 35 8 29 0 (R) (K)
2577.4383 2577.3353 40 8 30 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (66/256AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/79 matches (5%).
Acc. #: Species: HUMAN Name: Metaphase chromosomal protein 1 (33 kDa metaphase chromosomal protein)
Index: MW: 32982 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
2233.1343 2233.1805 -21 38 55 0 (R) (E)
2249.1736 2249.1754 -0.83 1Met-ox 38 55 0 (R)YFVMLVPVLYDMQQFLVK(E)
2265.1731 2265.1703 1.2 2Met-ox 38 55 0 (R)YFVMLVPVLYDMQQFLVK(E)
2281.1849 2281.0930 40 220 239 0 (K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (38/290AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.