MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: NCBInr.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-096
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 1318063
Molecular weight search (1000 - 100000 Da) selects 1263474 entries.
Full pI range: 1318063 entries.
Species search ( HUMAN MOUSE ) selects 180801 entries.
Combined molecular weight, pI and species searches select 121813 entries.
Pre searches select 121813 entries.

Data Set 1 Results

MS-Fit search selects 387 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/69(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	7.475e+004	12 (17)	40.0	17.4	-8.13	23.3		26153/8.5	M	UNREADABLE	gi\|8923390\|ref\|NP_060282.1\| hypothetical protein FLJ20420 [Homo sapiens]
2	1466	5 (7)	11.0	7.2	3.84	42.4		80509/5.5	M	HUMAN	Adseverin (Scinderin)
3	1466	5 (7)	11.0	7.2	3.84	42.4		80522/5.5		HOMO SAPIENS	unnamed protein product
4	1466	5 (7)	11.0	7.2	3.84	42.4		80538/5.4		HOMO SAPIENS	scinderin
5	442	4 (5)	14.0	5.8	7.62	44.9		52814/5.3	M	UNREADABLE	gi\|14916473\|ref\|NP_149119.1\| scinderin; adseverin; KIAA1905 protein [Homo sapiens]

1017311	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	.	.	.	x	.	x	.	.	x	x	.	.	x	x	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.
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857374	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.
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84378	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.

Detailed Results

1. 12/69 matches (17%).
Acc. #: Species: UNREADABLE Name: gi|8923390|ref|NP_060282.1| hypothetical protein FLJ20420 [Homo sapiens]
Acc. #: Species: HOMO SAPIENS Name: unnamed protein product
Acc. #: Species: HOMO SAPIENS Name: Similar to hypothetical protein FLJ20420
Acc. #: Species: HOMO SAPIENS Name: hypothetical protein FLJ20420
Index: MW: 26153 Da pI: 8.5

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1315.6375	1315.6283	7.0		148	157	1	(R) (V)
1351.6304	1351.6646	-25	pyroGlu	137	147	1	(K)QDAFYKEQLAR(L)
1368.6920	1368.6912	0.56		137	147	1	(K) (L)
1531.6980	1531.7215	-15		177	189	0	(R) (I)
1575.6914	1575.7179	-17		49	63	0	(R) (R)
1687.8497	1687.8226	16		176	189	1	(K) (I)
1707.8238	1707.8441	-12		10	24	0	(R) (G)
1731.7925	1731.8190	-15		49	64	1	(R) (R)
1765.8094	1765.8397	-17		152	165	1	(R) (A)
1817.9144	1817.9332	-10		190	203	1	(K) (C)
1863.9362	1863.9452	-4.8		9	24	1	(R) (G)
2034.0423	2034.0508	-4.1		10	27	1	(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (92/227AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/69 matches (7%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Acc. #: Species: HOMO SAPIENS Name: scinderin
Index: MW: 80509 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1578.7799 1578.8029 -15 434 447 0 (R) (D)
1588.7176 1588.7317 -8.9 312 324 0 (K) (N)
1857.9487 1857.9697 -11 206 222 0 (R) (V)
2190.1920 2190.1294 29 525 544 0 (R) (L)
2225.1402 2225.0839 25 597 615 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (83/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/69 matches (7%).
Acc. #: Species: HOMO SAPIENS Name: unnamed protein product
Index: MW: 80522 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1578.7799 1578.8029 -15 434 447 0 (R) (D)
1588.7176 1588.7317 -8.9 312 324 0 (K) (N)
1857.9487 1857.9697 -11 206 222 0 (R) (V)
2190.1920 2190.1294 29 525 544 0 (R) (L)
2225.1402 2225.0839 25 597 615 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (83/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/69 matches (7%).
Acc. #: Species: HOMO SAPIENS Name: scinderin
Index: MW: 80538 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1578.7799 1578.8029 -15 434 447 0 (R) (D)
1588.7176 1588.7317 -8.9 312 324 0 (K) (N)
1857.9487 1857.9697 -11 206 222 0 (R) (V)
2190.1920 2190.1294 29 525 544 0 (R) (L)
2225.1402 2225.0839 25 597 615 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (83/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/69 matches (5%).
Acc. #: Species: UNREADABLE Name: gi|14916473|ref|NP_149119.1| scinderin; adseverin; KIAA1905 protein [Homo sapiens]
Acc. #: Species: HOMO SAPIENS Name: unnamed protein product
Index: MW: 52814 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1578.7799 1578.8029 -15 187 200 0 (R) (D)
1588.7176 1588.7317 -8.9 65 77 0 (K) (N)
2190.1920 2190.1294 29 278 297 0 (R) (L)
2225.1402 2225.0839 25 350 368 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (66/468AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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