MS-Fit Search Results


Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-98
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 40000 Da) selects 62796 entries.
pI search (6.00 - 10.00) selects 66491 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 3943 entries.
Pre searches select 3943 entries.

Data Set 1 Results

MS-Fit search selects 859 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/150(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.449e+00412 (8)27.08.016.692.7 38497/6.7HUMANActivator 1 36 kDa subunit (Replication factor C 36 kDa subunit) (A1 36 kDa subunit) (RF-C 36 kDa subunit) (RFC36)
21.641e+0049 (6)26.06.0-16.559.9 31627/8.6MOUSESteroidogenic acute regulatory protein, mitochondrial precursor (StAR) (StARD1) (Luteinizing hormone-induced protein)
324489 (6)30.06.0-8.7778.8 35591/6.6MOUSEEstrogen sulfotransferase, testis isoform (Sulfotransferase, estrogen-preferring)
420079 (6)23.06.015.475.5 32237/7.2HUMANShort stature homeobox protein (Short stature homeobox containing protein) (Pseudoautosomal homeobox containing osteogenic protein)
5113210 (6)24.06.79.4173.0 38677/7.6MOUSEAnnexin II (Lipocortin II) (Calpactin I heavy chain) (Chromobindin 8) (P36) (Protein I) (Placental anticoagulant protein IV) (PAP-IV)
610756 (4)25.04.032.068.0 19818/6.7HUMANSorting nexin 24 (SBBI31)
79998 (5)33.05.38.9472.1 25702/7.6MOUSEGlutathione S-transferase Mu 3 (GST class-mu 3) (Glutathione S-transferase GT9.3)
889310 (6)21.06.7-7.2970.8 34013/9.9HUMANTricarboxylate transport protein, mitochondrial precursor (Citrate transport protein) (CTP) (Tricarboxylate carrier protein)
98869 (6)17.06.0-1.4577.0 39842/6.7MOUSEMO25 protein
108227 (4)21.04.710.190.5 33068/6.6HUMANTissue factor precursor (TF) (Coagulation factor III) (Thromboplastin) (CD142 antigen)
36799..................x.....x.....x...x.........................xx........x............x........x..............x........x..............................x..
120858..x.......x.............................x.......................................................x........x....x............................xx........x
4632......x...........x........................x.......x...........x..............x...............x...........................................x.........x.
17612...........x............x.........xx..................x....................x......................x...........................x.....................x.
105109..................x..............................x.....x.....................xx....x.............x........x............x....................x.........
60310....................................................................x.................x..x.x...........x......................................x.......
85815.x..........x..........................................x........................x........................x..............xx.......................x....
84409.x........x.......................x......x......x.................................x...............xx..........x..............................x........
24684..............................x.......x....................x................x...............x...........x.x....x............x.........................
72362...x.....................x.........................x......x..............x.....x..................................................................x...


Detailed Results

1. 12/150 matches (8%).
Acc. #: Species: HUMAN Name: Activator 1 36 kDa subunit (Replication factor C 36 kDa subunit) (A1 36 kDa subunit) (RF-C 36 kDa subunit) (RFC36)
Index: MW: 38497 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
885.4261885.4834-6519250(R) (Y)
897.4332897.4140213053110(K) (L)
906.4127906.4685-622912980(R) (I)
913.4291913.4089221Met-ox 3053110(K)MADIEYR(L)
993.5221993.4563661952030(K) (A)
1009.51881009.4512671Met-ox 1952030(K)VDISEDGMK(A)
1048.55591048.5791-221031120(R) (T)
1111.60221111.5635351861940(R) (V)
1165.64761165.5887511Met-ox 2042140(K)ALVTLSSGDMR(R)
1305.73471305.6949302042151(K) (A)
1372.72011372.7299-7.21Met-ox 1741850(R)FGPLTPELMVPR(L)
2237.34732237.20836247661(K) (T)

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The matched peptides cover 27% (94/340AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/150 matches (6%).
Acc. #: Species: MOUSE Name: Steroidogenic acute regulatory protein, mitochondrial precursor (StAR) (StARD1) (Luteinizing hormone-induced protein)
Index: MW: 31627 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
857.4351857.4595-28170(-) (L)
873.4019873.4544-601Met-ox 170(-)MFLATFK(L)
927.3975927.4358-418150(K) (H)
1201.69331201.7196-222382470(K) (G)
1300.61851300.6571-301211310(R) (L)
1316.69111316.6520301Met-ox 1211310(R)LEVVVDQPMDR(L)
1765.83161765.8770-261151(-) (H)
1791.85141791.84105.81Met-ox 1401541(R)MEAMGEWNPNVKEIK(V)
2705.35952705.2962231922161(R) (A)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 26% (76/284AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/150 matches (6%).
Acc. #: Species: MOUSE Name: Estrogen sulfotransferase, testis isoform (Sulfotransferase, estrogen-preferring)
Index: MW: 35591 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4642864.42164972780(K) (I)
864.4642864.4766-142522581(K) (G)
885.4261885.4327-7.41Met-ox 1251300(K)MIYLCR(N)
934.4372934.4787-441781841(K) (N)
964.5143964.49262379850(R) (N)
1014.48411014.5584-732142220(R) (I)
1090.55371090.5937-371131210(K) (N)
1182.65861182.6127391251331(K) (D)
1752.94071752.9186132592731(K) (E)
2384.10572384.1888-351341530(K) (S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 30% (90/295AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/150 matches (6%).
Acc. #: Species: HUMAN Name: Short stature homeobox protein (Short stature homeobox containing protein) (Pseudoautosomal homeobox containing osteogenic protein)
Index: MW: 32237 Da pI: 7.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
875.4046875.4587-622732800(K) (L)
897.4332897.388949pyroGlu 1751810(K)QENQMHK(G)
913.4291913.383850pyroGlu 1Met-ox 1751810(K)QENQMHK(G)
914.4637914.4154531751810(K) (G)
973.5551973.53182437450(R) (S)
1065.54401065.5468-2.6AcetN 1100(-)EELTAFVSK(S)
1232.65071232.6652-121611691(R) (A)
1487.83591487.7818361481601(R) (V)
2384.10572384.09813.21351531(R) (L)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 23% (69/292AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/150 matches (6%).
Acc. #: Species: MOUSE Name: Annexin II (Lipocortin II) (Calpactin I heavy chain) (Chromobindin 8) (P36) (Protein I) (Placental anticoagulant protein IV) (PAP-IV)
Index: MW: 38677 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
885.4261885.4616-402212270(R) (V)
957.4592957.4715-131Met-ox 2742811(R)LYDSMKGK(G)
976.5076976.4814271Met-ox 1461521(R)VYKEMYK(T)
1073.59051073.58435.838470(R) (T)
1090.55371090.5931-362963041(R) (S)
1111.60221111.55364469770(R) (R)
1225.62841225.5701481581680(K) (K)
1302.73681302.72697.638491(R) (G)
1423.82511423.7289681Met-ox 2913021(R)IMVSRSEVDMLK(I)
1791.85141791.8799-161201350(K) (T)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 24% (84/339AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/150 matches (4%).
Acc. #: Species: HUMAN Name: Sorting nexin 24 (SBBI31)
Index: MW: 19818 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1040.52931040.45367310170(R) (G)
1131.57121131.55871139470(R) (K)
1141.60711141.571631190(-) (Y)
1157.64211157.5665651Met-ox 190(-)MEVYIPSFR(Y)
1259.69201259.65373039481(R) (L)
1909.07061909.1064-19951101(K) (A)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 25% (43/169AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/150 matches (5%).
Acc. #: Species: MOUSE Name: Glutathione S-transferase Mu 3 (GST class-mu 3) (Glutathione S-transferase GT9.3)
Index: MW: 25702 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
856.4432856.4602-201Met-ox 1931991(K)KISAYMK(S)
877.4056877.4420-4144500(R) (F)
976.5076976.50057.31451520(K) (V)
1104.62391104.6570-302032110(R) (I)
1300.61851300.5632431Met-ox 33430(R)YVMGDAPNFDR(S)
1427.90091427.8262521231341(K) (M)
1434.84851434.8221182002111(K) (I)
1994.07561993.9911421531680(K) (M)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 33% (73/218AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/150 matches (6%).
Acc. #: Species: HUMAN Name: Tricarboxylate transport protein, mitochondrial precursor (Citrate transport protein) (CTP) (Tricarboxylate carrier protein)
Index: MW: 34013 Da pI: 9.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
856.4432856.427718pyroGlu 1911980(K)QGSNQAIR(F)
873.4019873.4543-601911980(K) (F)
913.4291913.4566-302482550(R) (Y)
929.4374929.4515-151Met-ox 2482550(R)MQGLEAHK(Y)
955.4781955.5253-492702771(K) (G)
1109.56301109.55269.464721(R) (Q)
1232.65071232.6210242482571(R) (N)
1234.70331234.7047-1.286970(R) (A)
1316.69111316.7228-241631731(R) (E)
1838.04021837.938355pyroGlu 1912061(K)QGSNQAIRFFVMTSLR(N)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 21% (66/311AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/150 matches (6%).
Acc. #: Species: MOUSE Name: MO25 protein
Index: MW: 39842 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
906.4127906.4606-5321280(K) (Q)
922.4611922.45566.01Met-ox 21280(K)ESMAVLEK(Q)
922.4611922.4854-262Met-ox 2582640(K)LMMNLLR(D)
991.5104991.50356.92412480(R) (Y)
1066.54301066.54210.8452600(K) (E)
1165.64761165.6073352Met-ox 2582661(K)LMMNLLRDK(S)
1277.76491277.6775681Met-ox 18281(K)NLKESMAVLEK(Q)
1302.73681302.717015971070(K) (R)
1320.67321320.7527-602983081(K) (F)
1458.80791458.8181-7.0971081(K) (Q)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 17% (60/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/150 matches (4%).
Acc. #: Species: HUMAN Name: Tissue factor precursor (TF) (Coagulation factor III) (Thromboplastin) (CD142 antigen)
Index: MW: 33068 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
859.3997859.4161-192882950(K) (-)
898.4365898.4423-6.4AcetN 180(-)ETPAWPR(V)
964.5143964.5216-7.51691760(R) (D)
987.4736987.47221.4180(-) (V)
1060.60601060.5275741551630(K) (T)
1095.57981095.6275-439180(R) (T)
2211.25702211.0981722142321(K) (K)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 21% (62/295AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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