MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-99
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 706 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.807e+008	16 (26)	61.0	26.7	36.9	32.8		26675/9.7		MOUSE	40S ribosomal protein S3
2	1.806e+008	17 (28)	65.0	28.3	35.9	32.8		26689/9.7		HUMAN	40S ribosomal protein S3
3	1.944e+004	6 (10)	18.0	10.0	35.0	28.9		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	1.148e+004	8 (13)	17.0	13.3	23.1	81.0		70970/8.9		HUMAN	Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase kinase 3) (MEK kinase 3) (MEKK 3)
5	8529	6 (10)	20.0	10.0	23.5	74.0		43516/5.2		MOUSE	Spectrin alpha chain, brain (Spectrin, non-erythroid alpha chain) (Alpha-II spectrin) (Fodrin alpha chain)

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Detailed Results

1. 16/60 matches (26%).
Acc. #: Species: MOUSE Name: 40S ribosomal protein S3
Index: MW: 26675 Da pI: 9.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
896.4887	896.4882	0.56		11	18	0	(K) (A)
1029.6522	1029.6308	21		46	54	0	(R) (T)
1092.6082	1092.5689	36		19	27	0	(K) (E)
1156.7342	1156.7054	25		66	75	1	(R) (R)
1302.7384	1302.6840	42		95	106	0	(R) (Y)
1423.7277	1423.6705	40		28	40	0	(R) (V)
1458.8678	1458.8320	24		202	214	0	(K) (D)
1470.8129	1470.7692	30		215	227	0	(K) (G)
1572.8184	1572.7586	38		77	90	0	(R) (V)
1584.0240	1583.9485	48		41	54	1	(R) (T)
1589.8507	1589.7998	32	1Met-ox	228	243	0	(K)GGKPEPPAMPQPVPTA(-)
1728.9428	1728.8597	48		76	90	1	(K) (V)
1970.1039	1970.0387	33		11	27	1	(K) (E)
2000.0928	2000.0129	40		77	94	1	(R) (G)
2484.3455	2484.1869	64	1Met-ox	152	173	0	(K)FVDGLMIHSGDPVNYYVDTAVR(H)
2910.7850	2910.5828	69		202	227	1	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 61% (149/243AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/60 matches (28%).
Acc. #: Species: HUMAN Name: 40S ribosomal protein S3
Index: MW: 26689 Da pI: 9.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
896.4887 896.4882 0.56 11 18 0 (K) (A)
1029.6522 1029.6308 21 46 54 0 (R) (T)
1092.6082 1092.5689 36 19 27 0 (K) (E)
1156.7342 1156.7054 25 66 75 1 (R) (R)
1173.6218 1173.5978 20 1Met-ox 188 197 0 (K)IMLPWDPTGK(I)
1302.7384 1302.6840 42 95 106 0 (R) (Y)
1423.7277 1423.6705 40 28 40 0 (R) (V)
1458.8678 1458.8320 24 202 214 0 (K) (D)
1470.8129 1470.7692 30 215 227 0 (K) (G)
1572.8184 1572.7586 38 77 90 0 (R) (V)
1584.0240 1583.9485 48 41 54 1 (R) (T)
1589.8507 1589.7998 32 1Met-ox 228 243 0 (K)GGKPEPPAMPQPVPTA(-)
1728.9428 1728.8597 48 76 90 1 (K) (V)
1970.1039 1970.0387 33 11 27 1 (K) (E)
2000.0928 2000.0129 40 77 94 1 (R) (G)
2484.3455 2484.1869 64 1Met-ox 152 173 0 (K)FVDGLMIHSGDPVNYYVDTAVR(H)
2910.7850 2910.5828 69 202 227 1 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 65% (159/243AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.6385 1179.6010 32 377 386 0 (K) (H)
1302.7384 1302.7018 28 393 403 1 (R) (V)
1302.7384 1302.7157 17 344 355 0 (R) (A)
1383.7285 1383.6909 27 186 197 0 (K) (V)
1475.8170 1475.7494 46 212 223 0 (K) (T)
1475.8170 1475.7858 21 200 211 0 (R) (W)
1994.0835 1993.9772 53 224 239 0 (R) (R)
2384.0831 2383.9524 55 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (116/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase kinase 3) (MEK kinase 3) (MEKK 3)
Index: MW: 70970 Da pI: 8.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
896.4887 896.4365 58 262 268 0 (R) (G)
994.5311 994.4958 36 234 242 1 (R) (S)
1405.7246 1405.6559 49 337 349 0 (R) (N)
1437.7367 1437.6471 62 126 138 0 (R) (Q)
1566.8235 1566.8393 -10 350 363 1 (R) (R)
1963.0244 1962.9972 14 418 432 1 (K) (D)
2083.1393 2083.0685 34 579 597 0 (K) (D)
2384.0831 2384.2219 -58 472 491 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (110/626AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Spectrin alpha chain, brain (Spectrin, non-erythroid alpha chain) (Alpha-II spectrin) (Fodrin alpha chain)
Index: MW: 43516 Da pI: 5.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1173.6218 1173.6115 8.7 356 366 0 (K) (H)
1475.8170 1475.7647 35 1 12 1 (-) (V)
1963.0244 1962.9773 24 52 68 0 (R) (R)
1970.1039 1969.9958 55 330 345 1 (R) (G)
2299.3036 2299.1669 59 97 117 0 (K) (K)
2427.1613 2427.2619 -41 97 118 1 (K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (78/382AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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