MS-Fit Search Results



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Parameters

Database searched: NCBInr.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-100
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 1318063
Molecular weight search (10000 - 100000 Da) selects 1107087 entries.
Full pI range: 1318063 entries.
Species search ( HUMAN MOUSE ) selects 180801 entries.
Combined molecular weight, pI and species searches select 102719 entries.
Pre searches select 102719 entries.

Data Set 1 Results

MS-Fit search selects 3631 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/52(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.843e+00613 (25)57.025.0-45.139.4 33297/9.8MUNREADABLEgi|6005854|ref|NP_009204.1| B-cell associated protein [Homo sapiens]
23.125e+00512 (23)52.023.1-46.739.4 33274/9.8MUNREADABLEgi|6671622|ref|NP_031557.1| B-cell receptor-associated protein 37 [Mus musculus]
31.376e+0047 (13)10.013.5-5.3687.6 64598/5.6MUNREADABLEgi|8923341|ref|NP_060256.1| hypothetical protein FLJ20366 [Homo sapiens]
41.229e+0046 (11)8.011.5-6.5191.3 80908/5.5MUNREADABLEgi|23943914|ref|NP_060050.1| TRABID protein [Homo sapiens]
571148 (15)50.015.4-48.344.8 23635/9.6HOMO SAPIENSB-cell receptor associated protein
760969...........x....x...x.xxx..xx....x....x....x.....xx.
503244...........x....x...x.xxx..xx....x.........x.....xx.
1030938x................................xx.......xx..x....x
490385......x............................x..xxx.........x.
350589...........x....x...x.xx...................x.....xx.



Detailed Results

1. 13/52 matches (25%).
Acc. #: Species: UNREADABLE Name: gi|6005854|ref|NP_009204.1| B-cell associated protein [Homo sapiens]
Acc. #: Species: HOMO SAPIENS Name: B-cell receptor associated protein
Acc. #: Species: MUS MUSCULUS Name: BAP
Acc. #: Species: HOMO SAPIENS Name: repressor of estrogen receptor activity
Acc. #: Species: HOMO SAPIENS Name: B-cell receptor-associated protein BAP37
Acc. #: Species: HOMO SAPIENS Name: D-prohibitin
Acc. #: Species: HOMO SAPIENS Name: B-cell associated protein
Index: MW: 33297 Da pI: 9.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1057.46341057.5390-722012090(K) (A)
1177.55601177.6329-651481570(K) (A)
1204.55821204.6360-651Met-ox 981070(K)DLQMVNISLR(V)
1210.66081210.7411-661321420(R) (S)
1215.55941215.6221-522252360(K) (M)
1217.52061217.5915-5838480(R) (A)
1232.60891232.6421-271Met-ox 1111(-)MAQNLKDLAGR(L)
1259.67891259.7476-5425370(K) (E)
1723.86231723.9212-3472840(R) (A)
1853.93861853.9874-2655710(R) (I)
1904.91451904.9653-271Met-ox 1081230(R)VLSRPNAQELPSMYQR(L)
2225.11172225.1454-152712890(R) (G)
2240.12382240.1814-261721911(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 57% (172/299AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/52 matches (23%).
Acc. #: Species: UNREADABLE Name: gi|6671622|ref|NP_031557.1| B-cell receptor-associated protein 37 [Mus musculus]
Acc. #: Species: MOUSE Name: B-cell receptor-associated protein BAP37
Acc. #: Species: MUS MUSCULUS Name: IgM B-cell receptor associated protein (BAP) 37
Index: MW: 33274 Da pI: 9.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1057.46341057.5390-722002080(K) (A)
1177.55601177.6329-651471560(K) (A)
1204.55821204.6360-651Met-ox 971060(K)DLQMVNISLR(V)
1210.66081210.7411-661311410(R) (S)
1215.55941215.6221-522242350(K) (M)
1217.52061217.5915-5838480(R) (A)
1232.60891232.6421-271Met-ox 1111(-)MAQNLKDLAGR(L)
1259.67891259.7476-5425370(K) (E)
1723.86231723.9212-3471830(R) (A)
1904.91451904.9653-271Met-ox 1071220(R)VLSRPNAQELPSMYQR(L)
2225.11172225.1454-152702880(R) (G)
2240.12382240.1814-261711901(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 52% (155/298AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/52 matches (13%).
Acc. #: Species: UNREADABLE Name: gi|8923341|ref|NP_060256.1| hypothetical protein FLJ20366 [Homo sapiens]
Acc. #: Species: HUMAN Name: Protein KIAA1472
Acc. #: Species: HOMO SAPIENS Name: unnamed protein product
Index: MW: 64598 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
864.3754864.4191-50170(-) (K)
1723.86231723.84718.847621(K) (Y)
1739.85251739.84206.01Met-ox 47621(K)KCTIGMVGEGSIQSSR(Y)
1888.80461888.9261-643013170(K) (D)
1904.91451904.9210-3.41Met-ox 3013170(K)LESLLQSMEMAHSGSLR(D)
1920.90821920.9159-4.02Met-ox 3013170(K)LESLLQSMEMAHSGSLR(D)
2283.15832282.99887024450(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (62/595AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/52 matches (11%).
Acc. #: Species: UNREADABLE Name: gi|23943914|ref|NP_060050.1| TRABID protein [Homo sapiens]
Acc. #: Species: HUMAN Name: TRABID protein
Acc. #: Species: HOMO SAPIENS Name: TRABID protein
Index: MW: 80908 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1008.42081008.4890-682432500(K) (K)
1837.85191837.8292125846000(R) (G)
1853.93861853.8241621Met-ox 5846000(R)GHFSALVAMENDGYGNR(G)
1857.91981857.9632-23pyroGlu 1Met-ox 3253410(R)QDMLAILLTEVSQQAAK(C)
1858.92611858.9948-373253410(R) (C)
2240.12382240.0909154394580(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (62/708AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/52 matches (15%).
Acc. #: Species: HOMO SAPIENS Name: B-cell receptor associated protein
Index: MW: 23635 Da pI: 9.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1057.46341057.5390-721131210(K) (A)
1177.55601177.6329-6560690(K) (A)
1204.55821204.6360-651Met-ox 10190(K)DLQMVNISLR(V)
1210.66081210.7411-6644540(R) (S)
1215.55941215.6221-521371480(K) (M)
1904.91451904.9653-271Met-ox 20350(R)VLSRPNAQELPSMYQR(L)
2225.11172225.1454-151832010(R) (G)
2240.12382240.1814-26841031(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 50% (107/211AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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