MS-Fit Search Results

MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-101
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (40000 - 70000 Da) selects 32612 entries.
pI search (9.00 - 14.00) selects 27092 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 790 entries.
Pre searches select 790 entries.

Data Set 1 Results

MS-Fit search selects 126 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/100(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.523e+004	7 (7)	20.0	7.0	-55.3	35.2		40841/9.4		MOUSE	RNA 3'-terminal phosphate cyclase-like protein
2	3255	6 (6)	14.0	6.0	-37.3	113		53734/9.1		HUMAN	Hypothetical protein DJ1198H6.1
3	3034	7 (7)	27.0	7.0	-56.7	52.0		45567/9.0		HUMAN	Mitochondrial 28S ribosomal protein S29 (S29mt) (MRP-S29) (Death-associated protein 3) (DAP-3) (Ionizing radiation resistance conferring protein)
4	1612	5 (5)	17.0	5.0	-49.0	38.5		40871/9.4		HUMAN	RNA 3'-terminal phosphate cyclase-like protein (HSPC338)
5	1044	6 (6)	28.0	6.0	-27.7	112		40982/9.3		HUMAN	WNT-1 proto-oncogene protein precursor

27972	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
109890	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
69173	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.
23592	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
102898	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 7/100 matches (7%).
Acc. #: Species: MOUSE Name: RNA 3'-terminal phosphate cyclase-like protein
Index: MW: 40841 Da pI: 9.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1033.4687	1033.5181	-48		333	340	0	(K) (V)
1303.5819	1303.6568	-57		341	351	0	(K) (G)
1585.7041	1585.8073	-65		119	133	0	(R) (A)
1851.8225	1851.8812	-32	AcetN	1	17	0	(-)ATQAHSLSYAGCNFLR(Q)
1940.8422	1940.9111	-36		1	17	0	(-) (Q)
2170.9210	2171.0847	-75		219	237	1	(K) (S)
2210.9887	2211.1524	-74		214	231	1	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (75/373AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/100 matches (6%).
Acc. #: Species: HUMAN Name: Hypothetical protein DJ1198H6.1
Index: MW: 53734 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.4620 973.4049 59 pyroGlu 133 140 0 (R)QTAEDCPR(M)
1065.4366 1065.5073 -66 387 395 0 (R) (D)
1302.5492 1302.6635 -88 166 174 0 (R) (R)
1393.6412 1393.6574 -12 376 386 0 (R) (N)
1585.7041 1585.8452 -89 34 46 0 (R) (R)
1851.8225 1851.8740 -28 116 131 0 (R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (66/461AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/100 matches (7%).
Acc. #: Species: HUMAN Name: Mitochondrial 28S ribosomal protein S29 (S29mt) (MRP-S29) (Death-associated protein 3) (DAP-3) (Ionizing radiation resistance conferring protein)
Index: MW: 45567 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1060.4926 1060.5362 -41 21 29 0 (R) (Q)
1193.5353 1193.6319 -81 204 212 1 (K) (R)
1434.6920 1434.7269 -24 178 189 0 (R) (N)
2015.8752 2016.0249 -74 214 232 1 (R) (V)
2367.1670 2367.2283 -26 pyroGlu 250 271 0 (R)QSSLGMFHLLVAVDGINALWGR(T)
2469.0879 2469.3003 -86 299 321 1 (K) (A)
2902.2879 2902.4746 -64 21 46 1 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (111/398AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/100 matches (5%).
Acc. #: Species: HUMAN Name: RNA 3'-terminal phosphate cyclase-like protein (HSPC338)
Index: MW: 40871 Da pI: 9.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1585.7041 1585.8073 -65 119 133 0 (R) (A)
1716.7543 1716.8202 -38 158 174 0 (R) (K)
1851.8225 1851.8812 -32 AcetN 1 17 0 (-)ATQAHSLSYAGCNFLR(Q)
1940.8422 1940.9111 -36 1 17 0 (-) (Q)
2210.9887 2211.1524 -74 214 231 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (67/373AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/100 matches (6%).
Acc. #: Species: HUMAN Name: WNT-1 proto-oncogene protein precursor
Index: MW: 40982 Da pI: 9.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1383.6083 1383.6909 -60 179 190 1 (R) (G)
1837.8683 1837.7454 67 pyroGlu 1Met-ox 216 230 1 (R)QECKCHGMSGSCTVR(T)
1986.8141 1986.9456 -66 295 313 1 (K) (A)
1990.8816 1991.0238 -71 34 51 0 (R) (S)
2367.1670 2367.1338 14 196 215 1 (R) (Q)
2532.9896 2533.1168 -50 314 335 1 (R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (106/370AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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