MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-106
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 292 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.123e+00710 (16)47.016.733.623.8 25899/6.9HUMANProteasome subunit alpha type 2 (Proteasome component C3) (Macropain subunit C3) (Multicatalytic endopeptidase complex subunit C3)
22.851e+0079 (15)41.015.033.825.2 25926/8.4MOUSEProteasome subunit alpha type 2 (Proteasome component C3) (Macropain subunit C3) (Multicatalytic endopeptidase complex subunit C3)
35.567e+00510 (16)20.016.740.015.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
444037 (11)14.011.71.6562.6 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
536045 (8)18.08.311.821.9 35620/9.2HUMANSideroflexin 1
76333x................x...x.x.x...............x..x....x..x..x....
45358x................x.....x.x...............x..x....x..x..x....
48753x......x.x...xx.x...........x...x......x...........x........
105637x.....x....x...................x.x...x....x.................
3672.....x...................xxx.......................x........



Detailed Results

1. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 2 (Proteasome component C3) (Macropain subunit C3) (Multicatalytic endopeptidase complex subunit C3)
Index: MW: 25899 Da pI: 6.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
895.4957895.45254854600(K) (S)
1478.78421478.7167465180(R) (L)
1504.87431504.8263322212341(R) (-)
1564.77241564.75829.171840(K) (V)
1580.82081580.7531431Met-ox 71840(K)HIGLVYSGMGPDYR(V)
2027.22782027.15413619390(K) (A)
2201.22152201.1342401781960(R) (E)
2357.29522357.2353251771961(K) (E)
2521.43012521.345534931130(K) (V)
2649.50152649.440423921131(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 47% (112/234AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/60 matches (15%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 2 (Proteasome component C3) (Macropain subunit C3) (Multicatalytic endopeptidase complex subunit C3)
Index: MW: 25926 Da pI: 8.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
895.4957895.45254854600(K) (S)
1478.78421478.7167465180(R) (L)
1564.77241564.75829.171840(K) (V)
1580.82081580.7531431Met-ox 71840(K)HIGLVYSGMGPDYR(V)
2027.22782027.15413619390(K) (A)
2201.22152201.1342401781960(R) (E)
2357.29522357.2353251771961(K) (E)
2521.43012521.345534931130(K) (V)
2649.50152649.440423921131(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (98/234AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
895.4957895.45974030371(R) (R)
895.4957895.45974031381(R) (S)
1179.65741179.6010483773860(K) (H)
1277.75561277.7105354734830(K) (T)
1383.75291383.6909451861970(K) (V)
1393.80181393.7327502782891(R) (D)
1475.82351475.7858262002110(R) (W)
1638.92021638.8604361861991(K) (F)
1716.93731716.8517504184320(K) (G)
1994.04791993.9772352242390(R) (R)
2384.03522383.9524355195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (130/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
895.4957895.5154-2238440(R) (N)
1157.62191157.5876303633720(R) (G)
1320.65111320.6800-222762860(R) (K)
1707.81581707.8964-473113251(R) (L)
1765.81221765.7741225996151(R) (A)
1941.01941940.9792213353501(K) (M)
2083.08892083.0249315115270(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (93/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/60 matches (8%).
Acc. #: Species: HUMAN Name: Sideroflexin 1
Index: MW: 35620 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1109.54241109.53804.016240(R) (A)
1580.82081580.7842232913050(K) (I)
1596.81361596.7791221Met-ox 2913050(K)SSMSVTSLEAELQAK(I)
1628.91731628.89721236491(R) (I)
2384.03522384.0404-2.2931120(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (58/322AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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