MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-108
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 470 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.575e+00612 (20)76.020.0-1.9641.2 21057/7.0HUMANPhosphatidylethanolamine-binding protein (PEBP) (Neuropolypeptide h3) (Hippocampal cholinergic neurostimulating peptide) (HCNP) (Raf kinase inhibitor protein) (RKIP)
21.309e+0047 (11)20.011.7-14.956.1 47651/5.3HUMANAlpha-1-antichymotrypsin precursor (ACT)
370006 (10)12.010.0-21.935.3 57597/9.3MOUSETranslation initiation factor eIF-2B delta subunit (eIF-2B GDP-GTP exchange factor)
465168 (13)11.013.3-14.848.7 80210/6.2MOUSEHeat shock protein 75 kDa, mitochondrial precursor (HSP 75) (Tumor necrosis factor type 1 receptor associated protein) (TRAP-1) (TNFR-associated protein 1)
533596 (10)10.010.0-7.2434.1 68021/8.2HUMANProtein-tyrosine phosphatase, non-receptor type 9 (Protein-tyrosine phosphatase MEG2) (PTPase-MEG2)
72462....x....x....xx..x....x...x........x.....x..x...........xx.
28609x.................x.xx.............x..x.........x...........
27575...............x.......x...x.x.x.....x......................
109614.........x.....x.x..x..x.....x...x........x.................
22670....................xx...x....x.x............x..............


Detailed Results

1. 12/60 matches (20%).
Acc. #: Species: HUMAN Name: Phosphatidylethanolamine-binding protein (PEBP) (Neuropolypeptide h3) (Hippocampal cholinergic neurostimulating peptide) (HCNP) (Raf kinase inhibitor protein) (RKIP)
Index: MW: 21057 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
937.4581937.4995-441801870(K) (-)
1117.51491117.5312-151331410(K) (S)
1455.71491455.7207-4.01Met-ox 83930(R)EWHHFLVVNMK(G)
1560.82391560.8273-2.263760(K) (K)
1632.79111632.7982-4.348620(K) (L)
1688.92941688.92234.263771(K) (D)
1708.89361708.9063-7.41201320(R) (C)
1774.88821774.88521.71Met-ox 81931(K)YREWHHFLVVNMK(G)
1949.96671949.945611941130(K) (G)
2083.98922083.9435221621790(R) (L)
2807.35682807.4191-221201411(R) (S)
3471.84583471.7185378390(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 76% (143/187AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Alpha-1-antichymotrypsin precursor (ACT)
Index: MW: 47651 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4516864.4831-361791850(K) (N)
1632.79111632.8229-192842970(K) (R)
1648.76441648.8178-321Met-ox 2842970(K)MEEVEAMLLPETLK(R)
1664.78241664.8127-182Met-ox 2842970(K)MEEVEAMLLPETLK(R)
1664.78241664.8495-401451581(K) (R)
1752.83671752.8808-252682830(K) (M)
1789.83741789.7604431Met-ox 2172300(K)WEMPFDPQDTHQSR(F)
2225.14582225.12439.742620(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (86/423AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Translation initiation factor eIF-2B delta subunit (eIF-2B GDP-GTP exchange factor)
Index: MW: 57597 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1560.82391560.8022141441570(R) (R)
1688.92941688.9699-243083221(R) (F)
1708.89361708.9532-354024180(K) (V)
1716.84611716.9033-331431571(K) (R)
1716.84611716.9033-331441581(R) (L)
1724.89731724.9481-291Met-ox 4024180(K)VLLGAHALLANGSVMSR(V)
1778.87251778.8938-124694840(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (64/524AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: Heat shock protein 75 kDa, mitochondrial precursor (HSP 75) (Tumor necrosis factor type 1 receptor associated protein) (TRAP-1) (TNFR-associated protein 1)
Index: MW: 80210 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1117.51491117.5642-4490981(K) (L)
1560.82391560.7918212943060(R) (D)
1580.75161580.7920-262652781(K) (Y)
1648.76441648.8375-442792920(K) (R)
1688.92941688.9124103293421(R) (S)
1716.84611716.8929-272933061(K) (D)
1732.88921732.88790.781Met-ox 2933061(K)RINTLQAIWMMDPK(D)
1949.96671949.9835-8.61992160(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (83/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase, non-receptor type 9 (Protein-tyrosine phosphatase MEG2) (PTPase-MEG2)
Index: MW: 68021 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1648.76441648.7852-135785930(K) (-)
1664.78241664.78011.41Met-ox 5785930(K)EGMVSSGQNLLAVESQ(-)
1706.89531706.9264-183954091(K) (R)
1722.88791722.9213-191Met-ox 3954091(K)VLVIVMTTRFEEGGR(R)
1729.95431729.9132244865011(R) (S)
2083.98922084.0275-184264430(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (65/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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