MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-110
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 385 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
17.995e+00617 (28)55.028.3-6.8434.6 22111/8.3HUMANPeroxiredoxin 1 (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2) (Proliferation-associated protein PAG) (Natural killer cell enhancing factor A) (NKEF-A)
21.318e+0048 (13)26.013.3-16.924.0 22177/8.3MOUSEPeroxiredoxin 1 (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2) (Osteoblast specific factor 3) (OSF-3) (Macrophage 23 kDa stress protein)
39536 (10)8.010.017.645.2 67355/6.3MOUSEBrown fat inducible thioesterase (BFIT) (Adipose associated thioesterase)
47866 (10)6.010.0-5.0943.1 77867/8.9MOUSELactotransferrin precursor (Lactoferrin)
57466 (10)8.010.0-3.4155.1 82106/6.3MOUSEExostosin-2 (Multiple exostoses protein 2 homolog)
19418........x....x..x......x...x...x.xx..x......xxxx..xxx.....x.
94972........x....x.........x...x...x..x.........xx..............
58297...........................x...x...x..........x....xx.......
26301.......x........x.........x.........x...x...........x.......
87494............................x.x...xx..x.................x...


Detailed Results

1. 17/60 matches (28%).
Acc. #: Species: HUMAN Name: Peroxiredoxin 1 (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2) (Proliferation-associated protein PAG) (Natural killer cell enhancing factor A) (NKEF-A)
Index: MW: 22111 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
894.4105894.4321-241211280(K) (G)
920.4827920.5093-291291360(R) (G)
980.4969980.5318-368160(K) (A)
1107.57981107.6050-231111200(R) (A)
1164.53631164.5723-3117270(K) (D)
1180.54541180.5672-181Met-ox 17270(K)ATAVMPDGQFK(D)
1194.63861194.6483-8.1pyroGlu 1411510(R)QITVNDLPVGR(S)
1196.63121196.6315-0.331591680(R) (H)
1211.69291211.6748151411510(R) (S)
1263.69921263.7061-5.51101201(K) (A)
1359.79481359.8000-3.91291401(R) (Q)
1621.83351621.82604.6pyroGlu 1Met-ox 941090(K)QGGLGPMNIPLVSDPK(R)
1638.86931638.8525101Met-ox 941090(K)QGGLGPMNIPLVSDPK(R)
1766.96481766.94759.81Met-ox 931091(K)KQGGLGPMNIPLVSDPK(R)
1777.92661777.9271-0.25pyroGlu 1Met-ox 941101(K)QGGLGPMNIPLVSDPKR(T)
1794.95361794.9536-0.0401Met-ox 941101(K)QGGLGPMNIPLVSDPKR(T)
2420.25852420.2033231691900(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 55% (111/199AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: Peroxiredoxin 1 (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2) (Osteoblast specific factor 3) (OSF-3) (Macrophage 23 kDa stress protein)
Index: MW: 22177 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
894.4105894.4321-241211280(K) (G)
920.4827920.5093-291291360(R) (G)
1107.57981107.6050-231111200(R) (A)
1164.53631164.5723-3117270(K) (D)
1180.54541180.5672-181Met-ox 17270(K)ATAVMPDGQFK(D)
1196.63121196.6315-0.331591680(R) (H)
1263.69921263.7061-5.51101201(K) (A)
1359.79481359.8000-3.91291401(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (52/199AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Brown fat inducible thioesterase (BFIT) (Adipose associated thioesterase)
Index: MW: 67355 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1164.53631164.5029292092180(K) (T)
1180.54541180.4978401Met-ox 2092180(K)DCSNMVPAEK(T)
1197.62551197.61627.71Met-ox 1100(-)MIQNVGNHLR(R)
1621.83351621.8412-4.83163290(R) (D)
1777.92661777.9423-8.83153291(R) (D)
1794.95361794.8775425105240(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (50/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Lactotransferrin precursor (Lactoferrin)
Index: MW: 77867 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
892.3395892.3736-383763810(K) (A)
980.4969980.5053-8.56826890(K) (L)
1150.61671150.6261-8.11081170(R) (N)
1199.63051199.62078.2pyroGlu 62710(R)QCIQAIVTNR(A)
1216.63461216.6472-1062710(R) (A)
1794.95361794.9060266546681(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (49/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Exostosin-2 (Multiple exostoses protein 2 homolog)
Index: MW: 82106 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1167.62281167.6374-122762850(K) (C)
1179.56421179.5978-291101(-) (G)
1196.63121196.5952304955040(K) (I)
1197.62551197.6342-7.36806900(K) (V)
1213.66551213.6291301Met-ox 6806900(K)FASVFGTMPLK(V)
2211.16122211.2324-321501681(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (60/718AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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