MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-111
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 567 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.095e+00813 (16)33.016.3-41.030.1 47232/4.9HUMANKeratin, type I cuticular HA1 (Hair keratin, type I HA1)
23.629e+00710 (12)27.012.5-40.234.5 46214/4.8HUMANKeratin, type I cuticular HA3-II (Hair keratin, type I HA3-II)
31.995e+00711 (13)26.013.8-39.530.8 45936/4.8HUMANKeratin, type I cuticular HA3-I (Hair keratin, type I HA3-I)
43.293e+0047 (8)12.08.8-12.467.5 71356/8.1HUMANDystrobrevin beta (Beta-dystrobrevin) (DTN-B)
53.196e+0047 (8)12.08.8-11.671.3 84792/6.7HUMANIntegrin beta-2 precursor (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 beta-subunit) (CD18) (Complement receptor C3 beta-subunit)
61.329e+0048 (10)9.010.0-0.31780.0 76079/6.6MOUSECoagulation factor XIII B chain precursor (Protein-glutamine gamma-glutamyltransferase B chain) (Transglutaminase B chain)
71.179e+0046 (7)11.07.5-32.284.7 98783/6.9HUMANSOX-13 protein (Type 1 diabetes autoantigen ICA12) (Islet cell antigen 12)
893406 (7)9.07.5-33.432.7 44689/4.7HUMANKeratin, type I cuticular HA4 (Hair keratin, type I HA4)
987546 (7)20.07.5-13.393.8 67889/9.0HUMANZinc finger protein 132
1076377 (8)14.08.8-1.6348.3 61942/8.7HUMANHIRA-interacting protein 3
103949......xxxx...........xx..x..x.........xx............x...............x.....x.....
56923......xxx................x..x..........x............x............x..x.....x.....
8664......xxx............xx.xx..x..........x............x...............x...........
103349........................x..x......x.......x.....................x.x.....x.......
18371.........x..............x............................x....x.....xxx.............
96505......xx..x....x.....................x......x.....................x.x...........
1313..............x.....................x..xx......................x.............x..
93481......xx.................x..x..........x............x...........................
48955........................x.........x....x.x..x.......................x...........
61172x.x........x......x.........................x.....................x.......x.....


Detailed Results

1. 13/80 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
Index: MW: 47232 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1153.50831153.5312-2058660(K) (L)
1169.51081169.5261-131Met-ox 58660(K)ETMQFLNDR(L)
1186.57181186.6068-2980890(R) (E)
1205.58031205.6312-421171261(K) (L)
1464.61211464.6818-482943060(R) (Y)
1465.67861465.7399-422302411(R) (E)
1487.71141487.7494-26pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.71351504.7760-423353460(R) (A)
1625.76531625.8499-522092230(R) (V)
1848.90741848.9932-462782930(R) (D)
2004.98542005.0943-542772931(R) (D)
2307.05752307.1832-542572760(K) (R)
2593.17982593.3473-653073290(R) (S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 33% (141/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/80 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA3-II (Hair keratin, type I HA3-II)
Index: MW: 46214 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1153.50831153.5312-2058660(K) (L)
1169.51081169.5261-131Met-ox 58660(K)ETMQFLNDR(L)
1186.57181186.6068-2980890(R) (E)
1487.71141487.7494-26pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.71351504.7760-423353460(R) (A)
1848.90741848.9932-462782930(R) (Y)
2004.98542005.0943-542772931(R) (Y)
2280.06302280.1836-532092290(R) (N)
2307.05752307.1832-542572760(K) (R)
2593.17982593.3473-653073290(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (112/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/80 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA3-I (Hair keratin, type I HA3-I)
Index: MW: 45936 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1153.50831153.5312-2058660(K) (L)
1169.51081169.5261-131Met-ox 58660(K)ETMQFLNDR(L)
1186.57181186.6068-2980890(R) (E)
1464.61211464.6818-482943060(R) (Y)
1465.67861465.7399-422302411(R) (E)
1485.73831485.8277-603173290(R) (S)
1487.71141487.7494-26pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.71351504.7760-423353460(R) (A)
1848.90741848.9932-462782930(R) (D)
2004.98542005.0943-542772931(R) (D)
2307.05752307.1832-542572760(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (106/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: Dystrobrevin beta (Beta-dystrobrevin) (DTN-B)
Index: MW: 71356 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1485.73831485.6770412742870(R) (E)
1501.57191501.6719-671Met-ox 2742870(R)GHAGGPHSNQHQMK(E)
1582.84601582.83427.524361(R) (T)
1871.84411871.9099-3564800(R) (L)
2273.97032273.9832-5.76056241(K) (D)
2289.95852289.9781-8.61Met-ox 6056241(K)ELHSAEEGAEEEEEKMQNGK(D)
2357.93272357.9789-202582730(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (80/627AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: Integrin beta-2 precursor (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 beta-subunit) (CD18) (Complement receptor C3 beta-subunit)
Index: MW: 84792 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1205.58031205.5625152862940(R) (R)
1485.73831485.8178-547257361(K) (R)
2028.80482028.9206-575445611(R) (C)
2135.98252135.9200295285430(K) (Y)
2273.97032273.9443116666840(R) (Y)
2280.06302280.1413-342542741(R) (L)
2289.95852289.93928.41Met-ox 6666840(R)DSEGCWVAYTLEQQDGMDR(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (95/769AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/80 matches (10%).
Acc. #: Species: MOUSE Name: Coagulation factor XIII B chain precursor (Protein-glutamine gamma-glutamyltransferase B chain) (Transglutaminase B chain)
Index: MW: 76079 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1153.50831153.4658371141230(R) (T)
1169.51081169.4607431Met-ox 1141230(R)MSYGCSSGYK(T)
1220.53321220.5662-2744530(K) (K)
1348.56981348.6611-6844541(K) (L)
1613.64571613.6940-301Met-ox 1141281(R)MSYGCSSGYKTTGGK(D)
1873.93171873.8649363643801(R) (G)
2289.95852289.9869-12pyroGlu 1912100(K)QTGEAECQANGWSLTPQCNK(L)
2307.05752307.0134191912100(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (63/668AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/80 matches (7%).
Acc. #: Species: HUMAN Name: SOX-13 protein (Type 1 diabetes autoantigen ICA12) (Islet cell antigen 12)
Index: MW: 98783 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1263.65331263.625522AcetN 1111(-)VNCTIKSEEK(K)
1595.68081595.6549165956070(K) (L)
1848.90741848.9794-395315470(R) (S)
1852.86401852.9467-457847970(R) (Y)
2227.98252228.1451-733133340(R) (A)
2766.11012766.3175-757617830(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (100/889AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/80 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA4 (Hair keratin, type I HA4)
Index: MW: 44689 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1153.50831153.5312-2058660(K) (L)
1169.51081169.5261-131Met-ox 58660(K)ETMQFLNDR(L)
1487.71141487.7494-26pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.71351504.7760-423353460(R) (A)
1848.90741848.9932-462782930(R) (D)
2004.98542005.0943-542772931(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (38/394AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/80 matches (7%).
Acc. #: Species: HUMAN Name: Zinc finger protein 132
Index: MW: 67889 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1485.73831485.7199123223340(R) (V)
1582.84601582.8090233503621(R) (V)
1848.90741848.8121523353490(K) (D)
1864.67891864.7958-632232370(R) (A)
1864.67891864.8070-695035170(R) (A)
1864.67891864.8070-693073210(R) (A)
1873.93171873.91658.12512651(K) (A)
2307.05752307.0603-1.21401590(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (121/589AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: HIRA-interacting protein 3
Index: MW: 61942 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.4710968.46892.240470(R) (Q)
1011.43281011.4682-354204280(R) (L)
1225.50301225.5184-133984100(R) (G)
1434.62971434.6461-111381491(K) (D)
1873.93171873.8528421912061(K) (T)
2289.95852289.9788-8.9961170(R) (N)
2593.17982593.148312941171(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (82/556AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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