MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-113
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 273 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/57(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.973e+008	16 (28)	55.0	28.1	21.8	26.7		35993/8.7		MOUSE	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
2	1.897e+008	16 (28)	53.0	28.1	21.8	26.7		37430/9.0		HUMAN	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
3	1.291e+005	11 (19)	45.0	19.3	19.4	57.8		36054/8.6		HUMAN	Glyceraldehyde 3-phosphate dehydrogenase, liver
4	1.421e+004	7 (12)	16.0	12.3	4.97	40.8		78011/8.7		HUMAN	Cytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
5	7109	6 (10)	11.0	10.5	2.28	68.8		98316/5.5		MOUSE	Band 4.1-like protein 1 (Neuronal protein 4.1) (4.1N)

81777	x	x	x	x	x	x	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.
119594	x	x	x	x	x	x	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.
117116	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	x	.	.	.	x	x	.	x	.	.	.	x
36351	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.
67253	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.

Detailed Results

1. 16/57 matches (28%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 35993 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1013.4446	1013.4441	0.50		192	201	0	(R) (G)
1050.4500	1050.4420	7.6		118	125	0	(R) (I)
1087.4977	1087.4849	12		27	34	0	(R) (L)
1188.6535	1188.6476	4.9		126	135	0	(K) (Q)
1220.5384	1220.5217	14	pyroGlu 1Met-ox	179	188	0	(R)QEMQEVQSSR(S)
1377.6521	1377.6300	16		202	216	0	(R) (G)
1410.7079	1410.6878	14		162	173	0	(K) (K)
1521.7915	1521.7405	34	1Met-ox	35	47	1	(K)LTDCVVMRDPASK(R)
1695.8056	1695.7655	24		142	156	0	(R) (I)
1798.9495	1798.9227	15		11	26	0	(K) (N)
1851.9341	1851.8666	36		141	156	1	(K) (I)
1880.0191	1879.9666	28		102	117	1	(K) (D)
1927.0704	1927.0177	27		10	26	1	(R) (N)
2205.9819	2205.9008	37	1Met-ox	314	338	0	(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.1415	2220.0712	32		118	135	1	(R) (Q)
2495.1593	2495.0401	48		227	254	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (188/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/57 matches (28%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 37430 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1013.4446 1013.4441 0.50 204 213 0 (R) (G)
1050.4500 1050.4420 7.6 130 137 0 (R) (I)
1087.4977 1087.4849 12 39 46 0 (R) (L)
1188.6535 1188.6476 4.9 138 147 0 (K) (Q)
1220.5384 1220.5217 14 pyroGlu 1Met-ox 191 200 0 (R)QEMQEVQSSR(S)
1377.6521 1377.6300 16 214 228 0 (R) (G)
1410.7079 1410.6878 14 174 185 0 (K) (K)
1521.7915 1521.7405 34 1Met-ox 47 59 1 (K)LTDCVVMRDPASK(R)
1695.8056 1695.7655 24 154 168 0 (R) (I)
1798.9495 1798.9227 15 23 38 0 (K) (N)
1851.9341 1851.8666 36 153 168 1 (K) (I)
1880.0191 1879.9666 28 114 129 1 (K) (D)
1927.0704 1927.0177 27 22 38 1 (R) (N)
2205.9819 2205.9008 37 1Met-ox 326 350 0 (R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.1415 2220.0712 32 130 147 1 (R) (Q)
2495.1593 2495.0401 48 239 266 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (188/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/57 matches (19%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1411.7368 1411.7909 -38 201 215 0 (R) (A)
1544.8419 1544.8107 20 235 248 0 (R) (L)
1613.9419 1613.9015 25 67 80 0 (K) (D)
1763.8486 1763.8029 26 310 323 0 (K) (V)
1862.0285 1861.9516 41 146 162 0 (K) (V)
2041.1567 2041.1082 24 67 84 1 (K) (I)
2211.1419 2211.2171 -34 235 254 1 (R) (Y)
2245.1775 2245.0997 35 2Met-ox 119 139 0 (R)VIISAPSADAPMFVMGVNHEK(Y)
2293.1213 2293.0335 38 1Met-ox 87 107 0 (K)WGDAGAEYVVESTGVFTTMEK(A)
2401.2660 2401.2008 27 2Met-ox 118 139 1 (K)RVIISAPSADAPMFVMGVNHEK(Y)
2611.4857 2611.3554 50 1Met-ox 163 186 0 (K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (151/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/57 matches (12%).
Acc. #: Species: HUMAN Name: Cytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
Index: MW: 78011 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1377.6521 1377.6228 21 238 247 0 (R) (K)
1410.7079 1410.6846 16 578 588 0 (K) (Y)
1777.8612 1777.8920 -17 373 387 0 (K) (L)
1927.0704 1927.0289 22 396 413 1 (K) (R)
2042.1551 2042.1398 7.5 656 672 1 (K) (D)
2245.1775 2245.2444 -30 415 435 1 (R) (W)
2550.3131 2550.2743 15 589 610 1 (K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (114/675AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/57 matches (10%).
Acc. #: Species: MOUSE Name: Band 4.1-like protein 1 (Neuronal protein 4.1) (4.1N)
Index: MW: 98316 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1662.8755 1662.8009 45 720 736 0 (R) (D)
1717.8427 1717.9026 -35 405 419 0 (R) (S)
1762.8122 1762.8652 -30 175 189 0 (K) (Y)
1798.9495 1798.9777 -16 1Met-ox 376 392 1 (R)LVSPEPPPKGFLVMGSK(F)
2041.1567 2041.0792 38 119 135 0 (R) (D)
2228.0509 2228.0253 11 48 66 0 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (100/879AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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