MS-Fit Search Results



..........

Parameters

Database searched: NCBInr.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): A006
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 1217808
Molecular weight search (10000 - 150000 Da) selects 1050575 entries.
Full pI range: 1217808 entries.
Species search ( HUMAN MOUSE ) selects 180062 entries.
Combined molecular weight, pI and species searches select 101957 entries.
Pre searches select 101957 entries.

Data Set 1 Results

MS-Fit search selects 21648 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/100(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.406e+01936 (36)41.036.027.545.0 106770/5.7HOMO SAPIENS(D42041) The ha1225 gene product is related to human alpha-glucosidase.
21.405e+01936 (36)41.036.027.545.0 106901/5.7HOMO SAPIENS(AJ000332) Glucosidase II
38.629e+01837 (37)40.037.025.152.3 109439/5.8MUNREADABLEgi|21361456|ref|NP_055425.2| (NM_014610) alpha glucosidase II alpha subunit; likely ortholog of mouse G2an alpha glucosidase 2, alpha neutral subunit [Homo sapiens]
44.165e+01120 (20)49.020.025.557.1 48631/6.1MHOMO SAPIENS(BC017433) Unknown (protein for MGC:2773)
53.391e+00717 (17)31.017.018.990.4 66018/8.2MHUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
1168981............................x...xxxx..xx.xx.xx..x.....xxxx...xxx.x.xx...xx.....x.x.xx.x.xxx.xx.x..x.
271744............................x...xxxx..xx.xx.xx..x.....xxxx...xxx.x.xx...xx.....x.x.xx.x.xxx.xx.x..x.
608............................x...xxxx..xxxxx.xx..x.....xxxx..xxxx.x.xx...xx.....x.x.xx.x.x.x.xx.x..x.
1026317................................x.xx..x..x............xx.x...xx....x....xx.....x...x..x.x.x.x.....x.
485104x..........x........................x....x.x..x..xxxx.....xx....x....x.x.............x.....x........



Detailed Results

1. 36/100 matches (36%).
Acc. #: Species: HOMO SAPIENS Name: (D42041) The ha1225 gene product is related to human alpha-glucosidase.
Index: MW: 106770 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
644.3564644.35206.84454490(R) (T)
699.3516699.4153-918058110(R) (W)
782.4213782.41616.74784830(R) (N)
788.4519788.430727pyroGlu 8578620(R)QEFLLR(R)
805.4854805.4572358578620(R) (R)
883.4774883.4426396026090(R) (F)
972.6154972.5842321641720(R) (G)
1066.59731066.58977.26536610(K) (W)
1071.53861071.4900454374440(R) (F)
1128.62121128.5802361731810(R) (A)
1145.68411145.64313651600(R) (A)
1174.66161174.6220341001081(R) (R)
1319.72511319.6894275906010(R) (A)
1335.72471335.6843301Met-ox 5906010(R)SGGMERPFVLAR(A)
1352.87351352.85171684950(K) (N)
1355.64181355.5881405585680(K) (D)
1593.79011593.7313376626730(R) (A)
1609.78231609.7262351Met-ox 6626730(R)WYQMGAYQPFFR(A)
1636.79421636.7495272172301(K) (T)
1680.99091680.9437281121261(R) (L)
1692.96991692.9186309299430(R) (-)
1696.88741696.8236384374491(R) (T)
1717.94741717.9026266846970(R) (D)
1740.97011740.9285249149280(R) (K)
1874.06661874.0037346836971(R) (D)
1927.93901927.8530453373530(K) (W)
1946.97051946.9037346106260(R) (I)
1959.92261959.8428412Met-ox 3373530(K)MMDYLQGSGETPQTDVR(W)
2071.13962071.0805297047190(R) (E)
2145.13042145.0551355695870(R) (Q)
2155.04672154.9903261852051(R) (D)
2161.12032161.0500321Met-ox 5695870(R)DVHNIYGLYVHMATADGLR(Q)
2390.37512390.2985327848040(K) (G)
2499.46782499.38233461830(R) (V)
2535.37022535.2996283773990(R) (W)
3010.56563010.4223488648900(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (391/943AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 36/100 matches (36%).
Acc. #: Species: HOMO SAPIENS Name: (AJ000332) Glucosidase II
Index: MW: 106901 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
644.3564644.35206.84464500(R) (T)
699.3516699.4153-918068120(R) (W)
782.4213782.41616.74794840(R) (N)
788.4519788.430727pyroGlu 8588630(R)QEFLLR(R)
805.4854805.4572358588630(R) (R)
883.4774883.4426396036100(R) (F)
972.6154972.5842321651730(R) (G)
1066.59731066.58977.26546620(K) (W)
1071.53861071.4900454384450(R) (F)
1128.62121128.5802361741820(R) (A)
1145.68411145.64313652610(R) (A)
1174.66161174.6220341011091(R) (R)
1319.72511319.6894275916020(R) (A)
1335.72471335.6843301Met-ox 5916020(R)SGGMERPFVLAR(A)
1352.87351352.85171685960(K) (N)
1355.64181355.5881405595690(K) (D)
1593.79011593.7313376636740(R) (A)
1609.78231609.7262351Met-ox 6636740(R)WYQMGAYQPFFR(A)
1636.79421636.7495272182311(K) (T)
1680.99091680.9437281131271(R) (L)
1692.96991692.9186309309440(R) (-)
1696.88741696.8236384384501(R) (T)
1717.94741717.9026266856980(R) (D)
1740.97011740.9285249159290(R) (K)
1874.06661874.0037346846981(R) (D)
1927.93901927.8530453383540(K) (W)
1946.97051946.9037346116270(R) (I)
1959.92261959.8428412Met-ox 3383540(K)MMDYLQGSGETPQTDVR(W)
2071.13962071.0805297057200(R) (E)
2145.13042145.0551355705880(R) (Q)
2155.04672154.9903261862061(R) (D)
2161.12032161.0500321Met-ox 5705880(R)DVHNIYGLYVHMATADGLR(Q)
2390.37512390.2985327858050(K) (G)
2499.46782499.38233462840(R) (V)
2535.37022535.2996283784000(R) (W)
3010.56563010.4223488658910(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (391/944AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 37/100 matches (37%).
Acc. #: Species: UNREADABLE Name: gi|21361456|ref|NP_055425.2| (NM_014610) alpha glucosidase II alpha subunit; likely ortholog of mouse G2an alpha glucosidase 2, alpha neutral subunit [Homo sapiens]
Acc. #: Species: HOMO SAPIENS Name: (AF144074) glucosidase II alpha subunit
Index: MW: 109439 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
644.3564644.35206.84684720(R) (T)
699.3516699.4153-918288340(R) (W)
782.4213782.41616.75015060(R) (N)
788.4519788.430727pyroGlu 8808850(R)QEFLLR(R)
805.4854805.4572358808850(R) (R)
883.4774883.4426396256320(R) (F)
972.6154972.5842321651730(R) (G)
1036.57671036.5540222052131(K) (D)
1066.59731066.58977.26766840(K) (W)
1071.53861071.4900454604670(R) (F)
1128.62121128.5802361741820(R) (A)
1145.68411145.64313652610(R) (A)
1174.66161174.6220341011091(R) (R)
1319.72511319.6894276136240(R) (A)
1335.72471335.6843301Met-ox 6136240(R)SGGMERPFVLAR(A)
1352.87351352.85171685960(K) (N)
1355.64181355.5881405815910(K) (D)
1486.77891486.8633-571922041(K) (N)
1593.79011593.7313376856960(R) (A)
1609.78231609.7262351Met-ox 6856960(R)WYQMGAYQPFFR(A)
1636.79421636.7495272402531(K) (T)
1680.99091680.9437281131271(R) (L)
1692.96991692.9186309529660(R) (-)
1696.88741696.8236384604721(R) (T)
1717.94741717.9026267077200(R) (D)
1740.97011740.9285249379510(R) (K)
1874.06661874.0037347067201(R) (D)
1927.93901927.8530453603760(K) (W)
1946.97051946.9037346336490(R) (I)
1959.92261959.8428412Met-ox 3603760(K)MMDYLQGSGETPQTDVR(W)
2071.13962071.0805297277420(R) (E)
2145.13042145.0551355926100(R) (Q)
2161.12032161.0500321Met-ox 5926100(R)DVHNIYGLYVHMATADGLR(Q)
2390.37512390.2985328078270(K) (G)
2499.46782499.38233462840(R) (V)
2535.37022535.2996284004220(R) (W)
3010.56563010.4223488879130(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (392/966AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 20/100 matches (20%).
Acc. #: Species: HOMO SAPIENS Name: (BC017433) Unknown (protein for MGC:2773)
Acc. #: Species: HOMO SAPIENS Name: (BC017435) Unknown (protein for MGC:4438)
Index: MW: 48631 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
699.3516699.4153-912902960(R) (W)
788.4519788.430727pyroGlu 3423470(R)QEFLLR(R)
805.4854805.4572353423470(R) (R)
883.4774883.44263987940(R) (F)
1066.59731066.58977.21381460(K) (W)
1319.72511319.68942775860(R) (A)
1335.72471335.6843301Met-ox 75860(R)SGGMERPFVLAR(A)
1355.64181355.58814043530(K) (D)
1593.79011593.7313371471580(R) (A)
1609.78231609.7262351Met-ox 1471580(R)WYQMGAYQPFFR(A)
1692.96991692.9186304144280(R) (-)
1717.94741717.9026261691820(R) (D)
1740.97011740.9285243994130(R) (K)
1874.06661874.0037341681821(R) (D)
1946.97051946.903734951110(R) (I)
2071.13962071.0805291892040(R) (E)
2145.13042145.05513554720(R) (Q)
2161.12032161.0500321Met-ox 54720(R)DVHNIYGLYVHMATADGLR(Q)
2390.37512390.2985322692890(K) (G)
3010.56563010.4223483493750(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (210/428AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 17/100 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Acc. #: Species: HOMO SAPIENS Name: (M98776) keratin 1
Acc. #: Species: HOMO SAPIENS Name: (AF237621) keratin 1
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
515.3001515.3306-594044070(R) (L)
547.2461547.2588-2339450(R) (F)
547.2461547.2952-901781811(K) (E)
832.4894832.48920.1975820(K) (G)
1066.59731066.5169752702771(K) (T)
1087.60511087.5285704121(R) (S)
1157.62181157.5149921Met-ox 4644720(R)DYQELMNTK(L)
1179.64561179.6010383773860(K) (H)
1277.77351277.7105494734830(K) (T)
1300.61141300.5302622582670(K) (N)
1302.68921302.7018-9.73934031(R) (V)
1302.68921302.7157-203443550(R) (A)
1383.71951383.6909211861970(K) (V)
1475.81371475.7494442122230(K) (T)
1475.81371475.7858192002110(R) (W)
1638.84911638.8604-6.91861991(K) (F)
1699.84491699.825112pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.91251716.8517354184320(K) (G)
1994.05301993.9772382242390(R) (R)
2384.02322383.9524305195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (203/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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