MS-Fit Search Results

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Sample ID (comment): HSGB01
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 2075 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.18e+008	26/100 (26%)	84674.5 / 4.94	HUMAN	HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86)
2	3.47e+006	22/100 (22%)	84788.6 / 4.93	MOUSE	HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86) (TUMOR SPECIFIC TRANSPLANTATION 86 KD ANTIGEN) (TSTA)
3	1.34e+006	19/100 (19%)	83295.0 / 4.97	HUMAN	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90)
4	4.47e+005	18/100 (18%)	83326.0 / 4.97	MOUSE	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA)
5	6.5e+004	13/100 (13%)	66555.8 / 7.93	HUMAN	G PROTEIN-COUPLED RECEPTOR KINASE GRK4 (ITI1)

Detailed Results

1. 26/100 matches (26%). 84674.5 Da, pI = 4.94. Acc. # P07900. HUMAN. HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1151.5393	1151.5584	-16.6665	284	292	(K)(T)
1168.5219	1168.5711	-42.0922	447	456	(K)(K)
1224.5714	1224.6265	-44.9553	490	499	(K)(D)
1235.5594	1235.6020	-34.4970	500	510	(K)(L)
1236.5823	1236.6377	-44.7736	347	356	(R)(K)
1249.5593	1249.5847	-20.2723	174	185	(R)(V)
1264.5911	1264.6438	-41.6740	346	355	(R)(K)
1296.6083	1296.6660	-44.5556	447	457	(K)(K)
1311.5225	1311.5705	-36.5574	192	201	(K)(R)
1348.6099	1348.6650	-40.8212	328	338	(K)(A)
1348.6099	1348.5877	16.4680	621	632	(R)DNSTMGYMAAKK(H)	2Met-ox
1364.6671	1364.7327	-48.0598	347	357	(R)(K)
1392.6796	1392.7388	-42.5441	346	356	(R)(K)
1513.7294	1513.7862	-37.5545	387	400	(R)(E)
1527.6707	1527.7443	-48.2304	315	327	(K)(H)
1544.7894	1544.7332	36.4128	547	559	(K)(K)
1566.6919	1566.6998	-5.0107	465	478	(R)YYTSASGDEMVSLK(D)	1Met-ox
1589.8016	1589.8764	-47.0017	75	87	(K)(T)
1595.8272	1595.7991	17.5960	419	431	(K)(E)
1765.7012	1765.7616	-34.2019	227	241	(R)(E)
1778.8679	1778.9481	-45.1159	210	224	(K)(E)
1786.8574	1786.9452	-49.1094	633	647	(K)(Q)
2014.9731	2015.0449	-35.6699	186	201	(K)(R)
2192.8995	2192.9207	-9.6592	251	269	(K)(K)
2255.8859	2255.9594	-32.5785	154	173	(K)(T)
2429.1044	2429.1885	-34.6164	368	386	(R)VFIMDNCEELIPEYLNFIR(G)	1Cys-am
2445.1129	2445.1834	-28.8402	368	386	(R)VFIMDNCEELIPEYLNFIR(G)	1Met-ox 1Cys-am

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 39% (287/732AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 26.0 Mean Error = -29.4289 ppm Data Tolerance = 44.8733 ppm Mean Missed Cleavages = 0.6

2. 22/100 matches (22%). 84788.6 Da, pI = 4.93. Acc. # P07901. MOUSE. HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86) (TUMOR SPECIFIC TRANSPLANTATION 86 KD ANTIGEN) (TSTA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1151.5393 1151.5584 -16.6665 285 293 (K)(T)
1168.5219 1168.5711 -42.0922 448 457 (K)(K)
1235.5594 1235.6020 -34.4970 501 511 (K)(L)
1236.5823 1236.6377 -44.7736 348 357 (R)(K)
1249.5593 1249.5847 -20.2723 174 185 (R)(V)
1264.5911 1264.6438 -41.6740 347 356 (R)(K)
1296.6083 1296.6660 -44.5556 448 458 (K)(K)
1311.5225 1311.5705 -36.5574 192 201 (K)(R)
1348.6099 1348.6650 -40.8212 329 339 (K)(A)
1348.6099 1348.5877 16.4680 622 633 (R)DNSTMGYMAAKK(H) 2Met-ox
1364.6671 1364.7327 -48.0598 348 358 (R)(K)
1392.6796 1392.7388 -42.5441 347 357 (R)(K)
1513.7294 1513.7862 -37.5545 388 401 (R)(E)
1544.7894 1544.7332 36.4128 548 560 (K)(K)
1566.6919 1566.6998 -5.0107 466 479 (R)YYTSASGDEMVSLK(D) 1Met-ox
1595.8272 1595.7991 17.5960 420 432 (K)(E)
1778.8679 1778.9481 -45.1159 210 224 (K)(E)
1786.8574 1786.9452 -49.1094 634 648 (K)(Q)
1808.8669 1808.9394 -40.0758 59 74 (K)(E)
2014.9731 2015.0449 -35.6699 186 201 (K)(R)
2255.8859 2255.9594 -32.5785 154 173 (K)(T)
2429.1044 2429.1885 -34.6164 369 387 (R)VFIMDNCEELIPEYLNFIR(G) 1Cys-am
2445.1129 2445.1834 -28.8402 369 387 (R)VFIMDNCEELIPEYLNFIR(G) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1151.5393	1151.5584	-16.6665	285	293	(K)(T)
1168.5219	1168.5711	-42.0922	448	457	(K)(K)
1235.5594	1235.6020	-34.4970	501	511	(K)(L)
1236.5823	1236.6377	-44.7736	348	357	(R)(K)
1249.5593	1249.5847	-20.2723	174	185	(R)(V)
1264.5911	1264.6438	-41.6740	347	356	(R)(K)
1296.6083	1296.6660	-44.5556	448	458	(K)(K)
1311.5225	1311.5705	-36.5574	192	201	(K)(R)
1348.6099	1348.6650	-40.8212	329	339	(K)(A)
1348.6099	1348.5877	16.4680	622	633	(R)DNSTMGYMAAKK(H)	2Met-ox
1364.6671	1364.7327	-48.0598	348	358	(R)(K)
1392.6796	1392.7388	-42.5441	347	357	(R)(K)
1513.7294	1513.7862	-37.5545	388	401	(R)(E)
1544.7894	1544.7332	36.4128	548	560	(K)(K)
1566.6919	1566.6998	-5.0107	466	479	(R)YYTSASGDEMVSLK(D)	1Met-ox
1595.8272	1595.7991	17.5960	420	432	(K)(E)
1778.8679	1778.9481	-45.1159	210	224	(K)(E)
1786.8574	1786.9452	-49.1094	634	648	(K)(Q)
1808.8669	1808.9394	-40.0758	59	74	(K)(E)
2014.9731	2015.0449	-35.6699	186	201	(K)(R)
2255.8859	2255.9594	-32.5785	154	173	(K)(T)
2429.1044	2429.1885	-34.6164	369	387	(R)VFIMDNCEELIPEYLNFIR(G)	1Cys-am
2445.1129	2445.1834	-28.8402	369	387	(R)VFIMDNCEELIPEYLNFIR(G)	1Met-ox 1Cys-am

The matched peptides cover 31% (233/733AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.0 Mean Error = -28.2873 ppm Data Tolerance = 46.4267 ppm Mean Missed Cleavages = 0.6

3. 19/100 matches (19%). 83295.0 Da, pI = 4.97. Acc. # P08238. HUMAN. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1151.5393 1151.5584 -16.6665 276 284 (K)(T)
1236.5823 1236.6377 -44.7736 338 347 (R)(K)
1249.5593 1249.6177 -46.6916 492 502 (K)(V)
1311.5225 1311.5705 -36.5574 187 196 (K)(R)
1348.6099 1348.6650 -40.8212 320 330 (K)(A)
1364.6671 1364.7327 -48.0598 338 348 (R)(K)
1513.7294 1513.7862 -37.5545 379 392 (R)(E)
1527.6707 1527.7443 -48.2304 307 319 (K)(H)
1544.7894 1544.8284 -25.2388 42 55 (R)(Y)
1544.7894 1544.7332 36.4128 539 551 (K)(K)
1595.8272 1595.7991 17.5960 411 423 (K)KCLELFSELAEDK(E) 1Cys-am
1808.8669 1808.9394 -40.0758 54 69 (K)(E)
1847.7113 1847.7976 -46.6664 292 306 (R)(S)
1910.9752 1911.0452 -36.6355 624 639 (K)(Q)
1993.9071 1993.9202 -6.5541 250 267 (K)(K)
2014.9731 2015.0449 -35.6699 181 196 (K)(R)
2192.8995 2192.9406 -18.7630 457 475 (R)YHTSQSGDEMTSLSEYVSR(M) 1Met-ox
2255.8859 2255.9594 -32.5785 149 168 (K)(A)
2388.0768 2388.1620 -35.6809 360 378 (R)VFIMDSCDELIPEYLNFIR(G) 1Cys-am
2404.0710 2404.1569 -35.7258 360 378 (R)VFIMDSCDELIPEYLNFIR(G) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1151.5393	1151.5584	-16.6665	276	284	(K)(T)
1236.5823	1236.6377	-44.7736	338	347	(R)(K)
1249.5593	1249.6177	-46.6916	492	502	(K)(V)
1311.5225	1311.5705	-36.5574	187	196	(K)(R)
1348.6099	1348.6650	-40.8212	320	330	(K)(A)
1364.6671	1364.7327	-48.0598	338	348	(R)(K)
1513.7294	1513.7862	-37.5545	379	392	(R)(E)
1527.6707	1527.7443	-48.2304	307	319	(K)(H)
1544.7894	1544.8284	-25.2388	42	55	(R)(Y)
1544.7894	1544.7332	36.4128	539	551	(K)(K)
1595.8272	1595.7991	17.5960	411	423	(K)KCLELFSELAEDK(E)	1Cys-am
1808.8669	1808.9394	-40.0758	54	69	(K)(E)
1847.7113	1847.7976	-46.6664	292	306	(R)(S)
1910.9752	1911.0452	-36.6355	624	639	(K)(Q)
1993.9071	1993.9202	-6.5541	250	267	(K)(K)
2014.9731	2015.0449	-35.6699	181	196	(K)(R)
2192.8995	2192.9406	-18.7630	457	475	(R)YHTSQSGDEMTSLSEYVSR(M)	1Met-ox
2255.8859	2255.9594	-32.5785	149	168	(K)(A)
2388.0768	2388.1620	-35.6809	360	378	(R)VFIMDSCDELIPEYLNFIR(G)	1Cys-am
2404.0710	2404.1569	-35.7258	360	378	(R)VFIMDSCDELIPEYLNFIR(G)	1Met-ox 1Cys-am

The matched peptides cover 33% (246/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.0 Mean Error = -28.9468 ppm Data Tolerance = 44.5563 ppm Mean Missed Cleavages = 0.7

4. 18/100 matches (18%). 83326.0 Da, pI = 4.97. Acc. # P11499. MOUSE. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1151.5393 1151.5584 -16.6665 276 284 (K)(T)
1236.5823 1236.6377 -44.7736 338 347 (R)(K)
1311.5225 1311.5705 -36.5574 187 196 (K)(R)
1348.6099 1348.6650 -40.8212 320 330 (K)(A)
1364.6671 1364.7327 -48.0598 338 348 (R)(K)
1513.7294 1513.7862 -37.5545 379 392 (R)(E)
1527.6707 1527.7443 -48.2304 307 319 (K)(H)
1544.7894 1544.8284 -25.2388 42 55 (R)(Y)
1544.7894 1544.7332 36.4128 539 551 (K)(K)
1595.8272 1595.7991 17.5960 411 423 (K)KCLELFSELAEDK(E) 1Cys-am
1808.8669 1808.9394 -40.0758 54 69 (K)(E)
1847.7113 1847.7976 -46.6664 292 306 (R)(S)
1910.9752 1911.0452 -36.6355 624 639 (K)(Q)
1993.9071 1993.9202 -6.5541 250 267 (K)(K)
2014.9731 2015.0449 -35.6699 181 196 (K)(R)
2192.8995 2192.9406 -18.7630 457 475 (R)YHTSQSGDEMTSLSEYVSR(M) 1Met-ox
2255.8859 2255.9594 -32.5785 149 168 (K)(A)
2388.0768 2388.1620 -35.6809 360 378 (R)VFIMDSCDELIPEYLNFIR(G) 1Cys-am
2404.0710 2404.1569 -35.7258 360 378 (R)VFIMDSCDELIPEYLNFIR(G) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1151.5393	1151.5584	-16.6665	276	284	(K)(T)
1236.5823	1236.6377	-44.7736	338	347	(R)(K)
1311.5225	1311.5705	-36.5574	187	196	(K)(R)
1348.6099	1348.6650	-40.8212	320	330	(K)(A)
1364.6671	1364.7327	-48.0598	338	348	(R)(K)
1513.7294	1513.7862	-37.5545	379	392	(R)(E)
1527.6707	1527.7443	-48.2304	307	319	(K)(H)
1544.7894	1544.8284	-25.2388	42	55	(R)(Y)
1544.7894	1544.7332	36.4128	539	551	(K)(K)
1595.8272	1595.7991	17.5960	411	423	(K)KCLELFSELAEDK(E)	1Cys-am
1808.8669	1808.9394	-40.0758	54	69	(K)(E)
1847.7113	1847.7976	-46.6664	292	306	(R)(S)
1910.9752	1911.0452	-36.6355	624	639	(K)(Q)
1993.9071	1993.9202	-6.5541	250	267	(K)(K)
2014.9731	2015.0449	-35.6699	181	196	(K)(R)
2192.8995	2192.9406	-18.7630	457	475	(R)YHTSQSGDEMTSLSEYVSR(M)	1Met-ox
2255.8859	2255.9594	-32.5785	149	168	(K)(A)
2388.0768	2388.1620	-35.6809	360	378	(R)VFIMDSCDELIPEYLNFIR(G)	1Cys-am
2404.0710	2404.1569	-35.7258	360	378	(R)VFIMDSCDELIPEYLNFIR(G)	1Met-ox 1Cys-am

The matched peptides cover 32% (235/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.0 Mean Error = -28.0128 ppm Data Tolerance = 44.9655 ppm Mean Missed Cleavages = 0.7

5. 13/100 matches (13%). 66555.8 Da, pI = 7.93. Acc. # P32298. HUMAN. G PROTEIN-COUPLED RECEPTOR KINASE GRK4 (ITI1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1169.5801 1169.6175 -31.9894 212 220 (K)(K)
1194.5856 1194.5499 29.9072 561 571 (R)GGCLTMVPSEK(E) 1Met-ox
1348.6099 1348.6717 -45.8197 560 571 (R)RGGCLTMVPSEK(E) 1Cys-am
1364.6671 1364.6666 0.3228 560 571 (R)RGGCLTMVPSEK(E) 1Met-ox 1Cys-am
1786.8574 1786.8434 7.8391 99 113 (R)(L)
1910.9752 1911.0023 -14.1818 433 450 (R)(D)
2014.9731 2014.9367 18.0709 289 305 (R)(E)
2204.9501 2205.0466 -43.7902 560 578 (R)(-)
2388.0768 2388.0827 -2.4771 457 476 (R)(D)
2404.0710 2404.0776 -2.7429 457 476 (R)LEANMLEPPFCPDPHAVYCK(D) 1Met-ox
2416.0008 2416.1140 -46.8437 457 476 (R)LEANMLEPPFCPDPHAVYCK(D) 2Cys-am
2461.0304 2461.0990 -27.8769 503 523 (R)(D)
2475.0533 2475.1147 -24.8211 503 523 (R)FATGCVSIPWQNEMIESGCFK(D) 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1169.5801	1169.6175	-31.9894	212	220	(K)(K)
1194.5856	1194.5499	29.9072	561	571	(R)GGCLTMVPSEK(E)	1Met-ox
1348.6099	1348.6717	-45.8197	560	571	(R)RGGCLTMVPSEK(E)	1Cys-am
1364.6671	1364.6666	0.3228	560	571	(R)RGGCLTMVPSEK(E)	1Met-ox 1Cys-am
1786.8574	1786.8434	7.8391	99	113	(R)(L)
1910.9752	1911.0023	-14.1818	433	450	(R)(D)
2014.9731	2014.9367	18.0709	289	305	(R)(E)
2204.9501	2205.0466	-43.7902	560	578	(R)(-)
2388.0768	2388.0827	-2.4771	457	476	(R)(D)
2404.0710	2404.0776	-2.7429	457	476	(R)LEANMLEPPFCPDPHAVYCK(D)	1Met-ox
2416.0008	2416.1140	-46.8437	457	476	(R)LEANMLEPPFCPDPHAVYCK(D)	2Cys-am
2461.0304	2461.0990	-27.8769	503	523	(R)(D)
2475.0533	2475.1147	-24.8211	503	523	(R)FATGCVSIPWQNEMIESGCFK(D)	1Cys-am

The matched peptides cover 20% (119/578AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.0 Mean Error = -14.1848 ppm Data Tolerance = 50.0317 ppm Mean Missed Cleavages = 0.8