MS-Fit Search Results

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Sample ID (comment): HSGB02
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 3237 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	9.96e+011	31/100 (31%)	70898.7 / 5.38	HUMAN	HEAT SHOCK COGNATE 71 KDA PROTEIN
2	2.66e+011	29/100 (29%)	70871.6 / 5.38	MOUSE	HEAT SHOCK COGNATE 71 KDA PROTEIN
3	8.6e+005	18/100 (18%)	70021.5 / 5.56	HUMAN	HEAT SHOCK-RELATED 70 KDA PROTEIN 2 (HEAT SHOCK 70 KDA PROTEIN 2)
4	4.49e+005	17/100 (17%)	69741.4 / 5.58	MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2)
5	1.31e+005	19/100 (19%)	68268.6 / 5.62	MOUSE	VACUOLAR ATP SYNTHASE CATALYTIC SUBUNIT A, UBIQUITOUS ISOFORM (V-ATPASE A SUBUNIT 1) (VACUOLAR PROTON PUMP ALPHA SUBUNIT 1) (V-ATPASE 69 KDA SUBUNIT 1)

Detailed Results

1. 31/100 matches (31%). 70898.7 Da, pI = 5.38. Acc. # P11142. HUMAN. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
687.3763	687.4041	-40.4508	50	56	(R)(N)
989.5172	989.5380	-21.0126	251	258	(K)(A)
989.5172	989.5267	-9.6605	510	517	(R)(M)
989.5172	989.4904	27.1111	562	569	(K)(I)
1081.5657	1081.5682	-2.3727	349	357	(K)(E)
1197.6741	1197.6632	9.1393	459	469	(K)(G)
1199.6715	1199.6748	-2.7379	160	171	(K)(I)
1228.6463	1228.6286	14.4530	26	36	(K)(T)
1251.6203	1251.6196	0.5746	237	246	(R)MVNHFIAEFK(R)	1Met-ox
1253.6211	1253.6166	3.5346	302	311	(R)(G)
1303.6148	1303.5993	11.9359	540	550	(K)(A)
1319.6567	1319.5942	47.3661	540	550	(K)NSLESYAFNMK(A)	1Met-ox
1410.6830	1410.6687	10.1158	77	88	(R)(H)
1426.6706	1426.6637	4.8823	77	88	(R)RFDDAVVQSDMK(H)	1Met-ox
1457.7833	1457.8038	-14.0642	598	609	(K)ELEKVCNPIITK(L)	1Cys-am
1480.7749	1480.7549	13.5642	300	311	(R)(G)
1481.7873	1481.8076	-13.7364	329	342	(K)(I)
1487.7207	1487.7018	12.7067	37	49	(R)(L)
1565.7669	1565.8328	-42.0765	349	361	(K)(S)
1616.8002	1616.7882	7.4547	113	126	(K)(M)
1653.8556	1653.8324	14.0561	89	102	(K)(V)
1669.8623	1669.8273	20.9607	89	102	(K)HWPFMVVNDAGRPK(V)	1Met-ox
1691.7547	1691.7261	16.9085	221	236	(K)(M)
1745.8256	1745.8095	9.2191	584	597	(K)(E)
1767.8172	1767.8587	-23.4931	513	526	(K)(A)
1837.9926	1838.0136	-11.3964	326	342	(K)(I)
1982.0388	1981.9983	20.4417	138	155	(K)(Q)
1982.0388	1981.9653	37.0996	518	533	(R)(D)
2044.0231	2044.0901	-32.7781	568	583	(K)QKILDKCNEIINWLDK(N)	1Cys-am
2260.2455	2260.1461	43.9728	362	384	(K)(S)
2277.2079	2277.1338	32.5351	4	25	(K)GPAVGIDLGTTYSCVGVFQHGK(V)	1Cys-am
2758.3505	2758.4086	-21.0593	562	583	(K)INDEDKQKILDKCNEIINWLDK(N)	1Cys-am
2774.4150	2774.3273	31.6128	424	447	(K)(A)
2997.4869	2997.4581	9.6291	273	299	(R)(A)

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The matched peptides cover 56% (367/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 31.0 Mean Error = 4.8363 ppm Data Tolerance = 44.8369 ppm Mean Missed Cleavages = 0.6

2. 29/100 matches (29%). 70871.6 Da, pI = 5.38. Acc. # P08109. MOUSE. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
687.3763 687.4041 -40.4508 50 56 (R)(N)
989.5172 989.5380 -21.0126 251 258 (K)(A)
989.5172 989.5267 -9.6605 510 517 (R)(M)
989.5172 989.4904 27.1111 562 569 (K)(I)
1081.5657 1081.5682 -2.3727 349 357 (K)(E)
1197.6741 1197.6632 9.1393 459 469 (K)(G)
1199.6715 1199.6748 -2.7379 160 171 (K)(I)
1228.6463 1228.6286 14.4530 26 36 (K)(T)
1251.6203 1251.6196 0.5746 237 246 (R)MVNHFIAEFK(R) 1Met-ox
1253.6211 1253.6166 3.5346 302 311 (R)(G)
1303.6148 1303.5993 11.9359 540 550 (K)(A)
1319.6567 1319.5942 47.3661 540 550 (K)NSLESYAFNMK(A) 1Met-ox
1410.6830 1410.6687 10.1158 77 88 (R)(H)
1426.6706 1426.6637 4.8823 77 88 (R)RFDDAVVQSDMK(H) 1Met-ox
1457.7833 1457.8038 -14.0642 598 609 (K)ELEKVCNPIITK(L) 1Cys-am
1480.7749 1480.7549 13.5642 300 311 (R)(G)
1481.7873 1481.8076 -13.7364 329 342 (K)(I)
1487.7207 1487.7018 12.7067 37 49 (R)(L)
1565.7669 1565.8328 -42.0765 349 361 (K)(S)
1616.8002 1616.7882 7.4547 113 126 (K)(M)
1653.8556 1653.8324 14.0561 89 102 (K)(V)
1669.8623 1669.8273 20.9607 89 102 (K)HWPFMVVNDAGRPK(V) 1Met-ox
1691.7547 1691.7261 16.9085 221 236 (K)(M)
1745.8256 1745.8095 9.2191 584 597 (K)(E)
1767.8172 1767.8587 -23.4931 513 526 (K)(A)
1837.9926 1838.0136 -11.3964 326 342 (K)(I)
1982.0388 1981.9983 20.4417 138 155 (K)(Q)
1982.0388 1981.9653 37.0996 518 533 (R)(D)
2260.2455 2260.1461 43.9728 362 384 (K)(S)
2277.2079 2277.1338 32.5351 4 25 (K)GPAVGIDLGTTYSCVGVFQHGK(V) 1Cys-am
2774.4150 2774.3273 31.6128 424 447 (K)(A)
2997.4869 2997.4581 9.6291 273 299 (R)(A)

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The matched peptides cover 54% (353/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.0 Mean Error = 6.8210 ppm Data Tolerance = 43.0661 ppm Mean Missed Cleavages = 0.5

3. 18/100 matches (18%). 70021.5 Da, pI = 5.56. Acc. # P54652. HUMAN. HEAT SHOCK-RELATED 70 KDA PROTEIN 2 (HEAT SHOCK 70 KDA PROTEIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
687.3763 687.4041 -40.4508 51 57 (R)(N)
1081.5657 1081.5682 -2.3727 352 360 (K)(E)
1228.6463 1228.6286 14.4530 27 37 (K)(T)
1253.6211 1253.6166 3.5346 305 314 (R)(G)
1457.7833 1457.7786 3.1893 601 612 (K)(L)
1480.7749 1480.7549 13.5642 303 314 (R)(G)
1487.7207 1487.7018 12.7067 38 50 (R)(L)
1565.7669 1565.8328 -42.0765 352 364 (K)(S)
1691.7547 1691.7261 16.9085 224 239 (K)(M)
1767.8172 1767.8336 -9.2658 516 529 (K)(S)
1837.9926 1837.9482 24.1741 74 89 (R)(H)
1994.0218 1994.0493 -13.8012 73 89 (K)(H)
1994.0218 1993.9231 49.5219 79 94 (K)(V)
2044.0231 2044.0313 -3.9868 110 127 (K)(M)
2044.0231 2044.0650 -20.4736 571 586 (K)(N)
2277.2079 2277.1338 32.5351 5 26 (R)(V)
2465.3143 2465.4091 -38.4680 326 348 (R)(I)
2757.4046 2757.3444 21.8294 530 553 (K)(Q)
2758.3505 2758.4198 -25.1316 565 586 (K)ISEQDKNKILDKCQEVINWLDR(N) 1Cys-am
2998.5433 2998.6577 -38.1522 161 189 (K)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
687.3763	687.4041	-40.4508	51	57	(R)(N)
1081.5657	1081.5682	-2.3727	352	360	(K)(E)
1228.6463	1228.6286	14.4530	27	37	(K)(T)
1253.6211	1253.6166	3.5346	305	314	(R)(G)
1457.7833	1457.7786	3.1893	601	612	(K)(L)
1480.7749	1480.7549	13.5642	303	314	(R)(G)
1487.7207	1487.7018	12.7067	38	50	(R)(L)
1565.7669	1565.8328	-42.0765	352	364	(K)(S)
1691.7547	1691.7261	16.9085	224	239	(K)(M)
1767.8172	1767.8336	-9.2658	516	529	(K)(S)
1837.9926	1837.9482	24.1741	74	89	(R)(H)
1994.0218	1994.0493	-13.8012	73	89	(K)(H)
1994.0218	1993.9231	49.5219	79	94	(K)(V)
2044.0231	2044.0313	-3.9868	110	127	(K)(M)
2044.0231	2044.0650	-20.4736	571	586	(K)(N)
2277.2079	2277.1338	32.5351	5	26	(R)(V)
2465.3143	2465.4091	-38.4680	326	348	(R)(I)
2757.4046	2757.3444	21.8294	530	553	(K)(Q)
2758.3505	2758.4198	-25.1316	565	586	(K)ISEQDKNKILDKCQEVINWLDR(N)	1Cys-am
2998.5433	2998.6577	-38.1522	161	189	(K)(G)

The matched peptides cover 40% (258/639AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.0 Mean Error = -2.0881 ppm Data Tolerance = 52.3917 ppm Mean Missed Cleavages = 1.3

4. 17/100 matches (17%). 69741.4 Da, pI = 5.58. Acc. # P17156. MOUSE. HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
687.3763 687.4041 -40.4508 51 57 (R)(N)
1081.5657 1081.5682 -2.3727 352 360 (K)(E)
1228.6463 1228.6286 14.4530 27 37 (K)(T)
1253.6211 1253.6166 3.5346 305 314 (R)(G)
1457.7833 1457.7786 3.1893 601 612 (K)(L)
1480.7749 1480.7549 13.5642 303 314 (R)(G)
1487.7207 1487.7018 12.7067 38 50 (R)(L)
1565.7669 1565.8328 -42.0765 352 364 (K)(S)
1691.7547 1691.7261 16.9085 224 239 (K)(M)
1767.8172 1767.8336 -9.2658 516 529 (K)(S)
1837.9926 1837.9482 24.1741 74 89 (R)(H)
1994.0218 1994.0493 -13.8012 73 89 (K)(H)
1994.0218 1993.9231 49.5219 79 94 (K)(V)
2044.0231 2044.0650 -20.4736 571 586 (K)(N)
2277.2079 2277.1338 32.5351 5 26 (R)(V)
2465.3143 2465.4091 -38.4680 326 348 (R)(I)
2758.3505 2758.4198 -25.1316 565 586 (K)ISEQDKNKILDKCQEVINWLDR(N) 1Cys-am
2998.5433 2998.6577 -38.1522 161 189 (K)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
687.3763	687.4041	-40.4508	51	57	(R)(N)
1081.5657	1081.5682	-2.3727	352	360	(K)(E)
1228.6463	1228.6286	14.4530	27	37	(K)(T)
1253.6211	1253.6166	3.5346	305	314	(R)(G)
1457.7833	1457.7786	3.1893	601	612	(K)(L)
1480.7749	1480.7549	13.5642	303	314	(R)(G)
1487.7207	1487.7018	12.7067	38	50	(R)(L)
1565.7669	1565.8328	-42.0765	352	364	(K)(S)
1691.7547	1691.7261	16.9085	224	239	(K)(M)
1767.8172	1767.8336	-9.2658	516	529	(K)(S)
1837.9926	1837.9482	24.1741	74	89	(R)(H)
1994.0218	1994.0493	-13.8012	73	89	(K)(H)
1994.0218	1993.9231	49.5219	79	94	(K)(V)
2044.0231	2044.0650	-20.4736	571	586	(K)(N)
2277.2079	2277.1338	32.5351	5	26	(R)(V)
2465.3143	2465.4091	-38.4680	326	348	(R)(I)
2758.3505	2758.4198	-25.1316	565	586	(K)ISEQDKNKILDKCQEVINWLDR(N)	1Cys-am
2998.5433	2998.6577	-38.1522	161	189	(K)(G)

The matched peptides cover 34% (216/633AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.0 Mean Error = -3.3114 ppm Data Tolerance = 54.0929 ppm Mean Missed Cleavages = 1.2

5. 19/100 matches (19%). 68268.6 Da, pI = 5.62. Acc. # P50516. MOUSE. VACUOLAR ATP SYNTHASE CATALYTIC SUBUNIT A, UBIQUITOUS ISOFORM (V-ATPASE A SUBUNIT 1) (VACUOLAR PROTON PUMP ALPHA SUBUNIT 1) (V-ATPASE 69 KDA SUBUNIT 1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
745.3918 745.3997 -10.5572 354 359 (R)(E)
888.5181 888.5307 -14.1824 565 571 (K)(E)
1145.5994 1145.6108 -9.8949 468 476 (K)(T)
1199.6715 1199.6636 6.6259 588 598 (K)(A)
1233.6597 1233.6479 9.5780 586 596 (K)(I)
1262.6804 1262.6785 1.4660 130 139 (R)(N)
1277.7094 1277.7265 -13.3886 164 173 (K)HKIMLPPRNR(G) 1Met-ox
1316.7088 1316.7439 -26.6674 221 232 (K)(V)
1319.6567 1319.6555 0.8709 553 564 (R)(I)
1687.8967 1687.8914 3.1797 308 323 (K)RTALVANTSNMPVAAR(E) 1Met-ox
1745.8256 1745.8169 4.9841 266 280 (K)YSNSDVIIYVGCGER(G) 1Cys-am
1749.8517 1749.8699 -10.4334 324 338 (R)(D)
1781.8980 1781.8532 25.0985 365 381 (R)(L)
1797.8860 1797.8482 21.0681 365 381 (R)LAEMPADSGYPAYLGAR(L) 1Met-ox
1800.8165 1800.8227 -3.4503 599 613 (K)ADYAQLLEDMQNAFR(S) 1Met-ox
2044.0231 2044.0115 5.6961 444 459 (K)HFPSVNWLISYSKYMR(A) 1Met-ox
2086.0497 2086.0245 12.0520 514 530 (K)(F)
2143.1489 2143.0500 46.1474 460 476 (R)(T)
2997.4869 2997.5996 -37.5822 479 506 (K)(I)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
745.3918	745.3997	-10.5572	354	359	(R)(E)
888.5181	888.5307	-14.1824	565	571	(K)(E)
1145.5994	1145.6108	-9.8949	468	476	(K)(T)
1199.6715	1199.6636	6.6259	588	598	(K)(A)
1233.6597	1233.6479	9.5780	586	596	(K)(I)
1262.6804	1262.6785	1.4660	130	139	(R)(N)
1277.7094	1277.7265	-13.3886	164	173	(K)HKIMLPPRNR(G)	1Met-ox
1316.7088	1316.7439	-26.6674	221	232	(K)(V)
1319.6567	1319.6555	0.8709	553	564	(R)(I)
1687.8967	1687.8914	3.1797	308	323	(K)RTALVANTSNMPVAAR(E)	1Met-ox
1745.8256	1745.8169	4.9841	266	280	(K)YSNSDVIIYVGCGER(G)	1Cys-am
1749.8517	1749.8699	-10.4334	324	338	(R)(D)
1781.8980	1781.8532	25.0985	365	381	(R)(L)
1797.8860	1797.8482	21.0681	365	381	(R)LAEMPADSGYPAYLGAR(L)	1Met-ox
1800.8165	1800.8227	-3.4503	599	613	(K)ADYAQLLEDMQNAFR(S)	1Met-ox
2044.0231	2044.0115	5.6961	444	459	(K)HFPSVNWLISYSKYMR(A)	1Met-ox
2086.0497	2086.0245	12.0520	514	530	(K)(F)
2143.1489	2143.0500	46.1474	460	476	(R)(T)
2997.4869	2997.5996	-37.5822	479	506	(K)(I)

The matched peptides cover 36% (226/617AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.0 Mean Error = 0.5584 ppm Data Tolerance = 37.6615 ppm Mean Missed Cleavages = 0.7