MS-Fit Search Results

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Sample ID (comment): HSGB03
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 3029 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.29e+011	31/85 (36%)	70052.8 / 5.48	HUMAN	HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2)
2	8.44e+007	22/85 (25%)	69994.6 / 5.46	MOUSE	HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2)
3	1.26e+006	16/85 (18%)	70695.9 / 5.82	MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN
4	8.17e+005	20/85 (23%)	70399.7 / 5.76	HUMAN	HEAT SHOCK 70 KDA PROTEIN 1-HOM (HSP70-HOM)
5	2.41e+005	13/85 (15%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)

Detailed Results

1. 31/85 matches (36%). 70052.8 Da, pI = 5.48. Acc. # P08107. HUMAN. HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
801.4406	801.4219	23.3705	452	458	(K)(F)
1109.5979	1109.5744	21.2295	349	357	(K)(D)
1137.5957	1137.5540	36.6508	525	533	(K)(E)
1183.6734	1183.6475	21.8940	459	469	(R)(G)
1197.6984	1197.6955	2.4004	160	171	(K)(I)
1228.6713	1228.6286	34.7789	26	36	(K)(T)
1232.6982	1232.6421	45.4923	448	458	(R)(F)
1253.6646	1253.7078	-34.5049	249	258	(K)(A)
1261.6879	1261.6581	23.5881	237	246	(R)(R)
1287.6449	1287.6043	31.5130	540	550	(K)(S)
1303.6347	1303.5993	27.2052	540	550	(K)NALESYAFNMK(S)	1Met-ox
1315.6450	1315.5993	34.7466	302	311	(R)(S)
1329.6620	1329.6149	35.4490	302	311	(R)FEELCSDLFR(S)	1Cys-am
1350.6984	1350.6728	19.0041	77	88	(R)(H)
1417.8054	1417.7592	32.5909	237	247	(R)(K)
1465.8541	1465.8127	28.2614	329	342	(K)(I)
1487.7486	1487.7018	31.4598	37	49	(R)(L)
1516.7453	1516.7720	-17.6054	527	539	(K)(N)
1542.7915	1542.7375	34.9857	300	311	(R)(S)
1556.8074	1556.7531	34.8422	300	311	(R)ARFEELCSDLFR(S)	1Cys-am
1556.8074	1556.8648	-36.8806	312	325	(R)(L)
1614.8166	1614.8089	4.7586	113	126	(K)(M)
1630.8437	1630.8038	24.4374	113	126	(K)AFYPEEISSMVLTK(M)	1Met-ox
1658.8793	1658.8502	17.5557	57	71	(K)(R)
1658.8793	1658.9553	-45.8189	498	512	(K)(E)
1675.7836	1675.7312	31.2581	221	236	(K)(L)
1680.9215	1680.8498	42.6297	89	102	(K)(V)
1687.9579	1687.9019	33.1714	172	187	(R)(T)
1876.9952	1876.9115	44.6034	574	589	(K)(D)
2279.2465	2279.1494	42.5965	4	25	(K)AAAIGIDLGTTYSCVGVFQHGK(V)	1Cys-am
2786.4847	2786.3637	43.4253	424	447	(K)(A)
2786.4847	2786.4035	29.1567	536	561	(R)(I)
3260.7454	3260.6302	35.3413	127	155	(K)(Q)
3276.7873	3276.6251	49.4967	127	155	(K)MKEIAEAYLGYPVTNAVITVPAYFNDSQR(Q)	1Met-ox

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The matched peptides cover 55% (358/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 36.5 Mean Error = 23.0319 ppm Data Tolerance = 47.3457 ppm Mean Missed Cleavages = 0.6

2. 22/85 matches (25%). 69994.6 Da, pI = 5.46. Acc. # P17879. MOUSE. HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
801.4406 801.4219 23.3705 452 458 (R)(F)
1109.5979 1109.5744 21.2295 349 357 (K)(D)
1137.5957 1137.5540 36.6508 525 533 (R)(D)
1179.6321 1179.6122 16.8587 103 112 (K)(S)
1183.6734 1183.6475 21.8940 459 469 (R)(G)
1197.6984 1197.6955 2.4004 160 171 (K)(I)
1228.6713 1228.6286 34.7789 26 36 (K)(T)
1253.6646 1253.7078 -34.5049 249 258 (K)(A)
1287.6449 1287.6043 31.5130 540 550 (K)(S)
1303.6347 1303.5993 27.2052 540 550 (K)NALESYAFNMK(S) 1Met-ox
1315.6450 1315.5993 34.7466 302 311 (R)(G)
1329.6620 1329.6149 35.4490 302 311 (R)FEELCSDLFR(G) 1Cys-am
1487.7486 1487.7018 31.4598 37 49 (R)(L)
1542.7915 1542.7375 34.9857 300 311 (R)(G)
1556.8074 1556.7531 34.8422 300 311 (R)ARFEELCSDLFR(G) 1Cys-am
1614.8166 1614.8089 4.7586 113 126 (R)(M)
1630.8437 1630.8038 24.4374 113 126 (R)SFFPEEISSMVLTK(M) 1Met-ox
1658.8793 1658.8502 17.5557 57 71 (K)(R)
1658.8793 1658.9553 -45.8189 498 512 (K)(E)
1675.7836 1675.7312 31.2581 221 236 (K)(L)
1687.9579 1687.9019 33.1714 172 187 (R)(T)
2771.4476 2771.3423 38.0197 527 550 (K)AEDEVQRDRVAAKNALESYAFNMK(S) 1Met-ox
2786.4847 2786.4035 29.1567 536 561 (R)VAAKNALESYAFNMKSAVEDEGLKGK(L) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
801.4406	801.4219	23.3705	452	458	(R)(F)
1109.5979	1109.5744	21.2295	349	357	(K)(D)
1137.5957	1137.5540	36.6508	525	533	(R)(D)
1179.6321	1179.6122	16.8587	103	112	(K)(S)
1183.6734	1183.6475	21.8940	459	469	(R)(G)
1197.6984	1197.6955	2.4004	160	171	(K)(I)
1228.6713	1228.6286	34.7789	26	36	(K)(T)
1253.6646	1253.7078	-34.5049	249	258	(K)(A)
1287.6449	1287.6043	31.5130	540	550	(K)(S)
1303.6347	1303.5993	27.2052	540	550	(K)NALESYAFNMK(S)	1Met-ox
1315.6450	1315.5993	34.7466	302	311	(R)(G)
1329.6620	1329.6149	35.4490	302	311	(R)FEELCSDLFR(G)	1Cys-am
1487.7486	1487.7018	31.4598	37	49	(R)(L)
1542.7915	1542.7375	34.9857	300	311	(R)(G)
1556.8074	1556.7531	34.8422	300	311	(R)ARFEELCSDLFR(G)	1Cys-am
1614.8166	1614.8089	4.7586	113	126	(R)(M)
1630.8437	1630.8038	24.4374	113	126	(R)SFFPEEISSMVLTK(M)	1Met-ox
1658.8793	1658.8502	17.5557	57	71	(K)(R)
1658.8793	1658.9553	-45.8189	498	512	(K)(E)
1675.7836	1675.7312	31.2581	221	236	(K)(L)
1687.9579	1687.9019	33.1714	172	187	(R)(T)
2771.4476	2771.3423	38.0197	527	550	(K)AEDEVQRDRVAAKNALESYAFNMK(S)	1Met-ox
2786.4847	2786.4035	29.1567	536	561	(R)VAAKNALESYAFNMKSAVEDEGLKGK(L)	1Met-ox

The matched peptides cover 32% (208/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.9 Mean Error = 21.1051 ppm Data Tolerance = 43.2542 ppm Mean Missed Cleavages = 0.7

3. 16/85 matches (18%). 70695.9 Da, pI = 5.82. Acc. # P16627. MOUSE. HEAT SHOCK-RELATED 70 KD PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
801.4406 801.4219 23.3705 454 460 (R)(F)
1183.6734 1183.6475 21.8940 461 471 (R)(G)
1197.6984 1197.6955 2.4004 162 173 (K)(I)
1228.6713 1228.6286 34.7789 28 38 (K)(T)
1228.6713 1228.6901 -15.3068 314 324 (R)(D)
1253.6646 1253.7078 -34.5049 251 260 (K)(A)
1287.6449 1287.6043 31.5130 542 552 (K)(S)
1303.6347 1303.5993 27.2052 542 552 (K)NALESYAFNMK(S) 1Met-ox
1465.8541 1465.8491 3.4386 331 344 (K)(I)
1480.7739 1480.7912 -11.7196 348 359 (K)(D)
1487.7486 1487.7018 31.4598 39 51 (R)(L)
1542.7915 1542.8491 -37.3887 314 327 (R)(M)
1614.8166 1614.8089 4.7586 115 128 (K)(M)
1630.8437 1630.8038 24.4374 115 128 (K)AFYPEEISSMVLTK(M) 1Met-ox
1658.8793 1658.9553 -45.8189 500 514 (K)(E)
1675.7836 1675.7312 31.2581 223 238 (K)(L)
2786.4847 2786.3637 43.4253 426 449 (K)(A)
2786.4847 2786.4035 29.1567 538 563 (K)IAAKNALESYAFNMKSAVGDEGLKDK(I) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
801.4406	801.4219	23.3705	454	460	(R)(F)
1183.6734	1183.6475	21.8940	461	471	(R)(G)
1197.6984	1197.6955	2.4004	162	173	(K)(I)
1228.6713	1228.6286	34.7789	28	38	(K)(T)
1228.6713	1228.6901	-15.3068	314	324	(R)(D)
1253.6646	1253.7078	-34.5049	251	260	(K)(A)
1287.6449	1287.6043	31.5130	542	552	(K)(S)
1303.6347	1303.5993	27.2052	542	552	(K)NALESYAFNMK(S)	1Met-ox
1465.8541	1465.8491	3.4386	331	344	(K)(I)
1480.7739	1480.7912	-11.7196	348	359	(K)(D)
1487.7486	1487.7018	31.4598	39	51	(R)(L)
1542.7915	1542.8491	-37.3887	314	327	(R)(M)
1614.8166	1614.8089	4.7586	115	128	(K)(M)
1630.8437	1630.8038	24.4374	115	128	(K)AFYPEEISSMVLTK(M)	1Met-ox
1658.8793	1658.9553	-45.8189	500	514	(K)(E)
1675.7836	1675.7312	31.2581	223	238	(K)(L)
2786.4847	2786.3637	43.4253	426	449	(K)(A)
2786.4847	2786.4035	29.1567	538	563	(K)IAAKNALESYAFNMKSAVGDEGLKDK(I)	1Met-ox

The matched peptides cover 31% (199/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.8 Mean Error = 9.1310 ppm Data Tolerance = 55.0233 ppm Mean Missed Cleavages = 0.8

4. 20/85 matches (23%). 70399.7 Da, pI = 5.76. Acc. # P34931. HUMAN. HEAT SHOCK 70 KDA PROTEIN 1-HOM (HSP70-HOM).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
801.4406 801.4219 23.3705 454 460 (K)(F)
1112.5768 1112.5662 9.5740 520 528 (R)MVLDAEKYK(A) 1Met-ox
1137.5957 1137.5540 36.6508 527 535 (K)(E)
1183.6734 1183.6475 21.8940 461 471 (R)(G)
1197.6984 1197.6955 2.4004 162 173 (K)(I)
1228.6713 1228.6286 34.7789 28 38 (K)(T)
1232.6982 1232.6421 45.4923 450 460 (R)(F)
1253.6646 1253.7078 -34.5049 251 260 (K)(A)
1287.6449 1287.6043 31.5130 542 552 (K)(S)
1303.6347 1303.5993 27.2052 542 552 (K)NALESYAFNMK(S) 1Met-ox
1389.7394 1389.7589 -14.0644 564 575 (K)(C)
1465.8541 1465.8491 3.4386 331 344 (K)(I)
1487.7486 1487.7018 31.4598 39 51 (R)(L)
1614.8166 1614.8089 4.7586 115 128 (K)(L)
1630.8437 1630.8038 24.4374 115 128 (K)AFYPEEISSMVLTK(L) 1Met-ox
1675.7836 1675.7312 31.2581 223 238 (K)(L)
2706.2890 2706.3925 -38.2432 1 27 (-)ATAKGIAIGIDLGTTYSCVGVFQHGK(V) Acet N
2771.4476 2771.3674 28.9437 529 552 (K)AEDEVQREKIAAKNALESYAFNMK(S) 1Met-ox
2786.4847 2786.3637 43.4253 426 449 (K)(A)
2786.4847 2786.4399 16.0983 538 563 (K)IAAKNALESYAFNMKSVVSDEGLKGK(I) 1Met-ox
3001.6606 3001.5610 33.1810 79 104 (R)KFNDPVVQADMKLWPFQVINEGGKPK(V) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
801.4406	801.4219	23.3705	454	460	(K)(F)
1112.5768	1112.5662	9.5740	520	528	(R)MVLDAEKYK(A)	1Met-ox
1137.5957	1137.5540	36.6508	527	535	(K)(E)
1183.6734	1183.6475	21.8940	461	471	(R)(G)
1197.6984	1197.6955	2.4004	162	173	(K)(I)
1228.6713	1228.6286	34.7789	28	38	(K)(T)
1232.6982	1232.6421	45.4923	450	460	(R)(F)
1253.6646	1253.7078	-34.5049	251	260	(K)(A)
1287.6449	1287.6043	31.5130	542	552	(K)(S)
1303.6347	1303.5993	27.2052	542	552	(K)NALESYAFNMK(S)	1Met-ox
1389.7394	1389.7589	-14.0644	564	575	(K)(C)
1465.8541	1465.8491	3.4386	331	344	(K)(I)
1487.7486	1487.7018	31.4598	39	51	(R)(L)
1614.8166	1614.8089	4.7586	115	128	(K)(L)
1630.8437	1630.8038	24.4374	115	128	(K)AFYPEEISSMVLTK(L)	1Met-ox
1675.7836	1675.7312	31.2581	223	238	(K)(L)
2706.2890	2706.3925	-38.2432	1	27	(-)ATAKGIAIGIDLGTTYSCVGVFQHGK(V)	Acet N
2771.4476	2771.3674	28.9437	529	552	(K)AEDEVQREKIAAKNALESYAFNMK(S)	1Met-ox
2786.4847	2786.3637	43.4253	426	449	(K)(A)
2786.4847	2786.4399	16.0983	538	563	(K)IAAKNALESYAFNMKSVVSDEGLKGK(I)	1Met-ox
3001.6606	3001.5610	33.1810	79	104	(R)KFNDPVVQADMKLWPFQVINEGGKPK(V)	1Met-ox

The matched peptides cover 38% (245/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.5 Mean Error = 17.2889 ppm Data Tolerance = 46.3347 ppm Mean Missed Cleavages = 0.9

5. 13/85 matches (15%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3242 515.3306 -12.2934 404 407 (R)(L)
1179.6321 1179.6010 26.3819 377 386 (K)(H)
1277.7318 1277.7105 16.6811 473 483 (K)(T)
1300.5943 1300.5302 49.2610 258 267 (K)(N)
1383.7160 1383.6909 18.1373 186 197 (K)(V)
1475.8085 1475.7858 15.3663 200 211 (R)(W)
1475.8085 1475.7494 40.0223 212 223 (K)(T)
1487.7486 1487.8043 -37.4012 240 251 (R)(L)
1687.9579 1687.9707 -7.5583 66 82 (R)(G)
1716.8995 1716.8517 27.8706 418 432 (K)(G)
1860.9628 1860.9667 -2.0785 242 257 (R)(N)
1994.0322 1993.9772 27.5910 224 239 (R)(R)
2384.0542 2383.9524 42.6959 519 549 (R)(G)
2771.4476 2771.4288 6.8047 417 441 (K)(N)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
515.3242	515.3306	-12.2934	404	407	(R)(L)
1179.6321	1179.6010	26.3819	377	386	(K)(H)
1277.7318	1277.7105	16.6811	473	483	(K)(T)
1300.5943	1300.5302	49.2610	258	267	(K)(N)
1383.7160	1383.6909	18.1373	186	197	(K)(V)
1475.8085	1475.7858	15.3663	200	211	(R)(W)
1475.8085	1475.7494	40.0223	212	223	(K)(T)
1487.7486	1487.8043	-37.4012	240	251	(R)(L)
1687.9579	1687.9707	-7.5583	66	82	(R)(G)
1716.8995	1716.8517	27.8706	418	432	(K)(G)
1860.9628	1860.9667	-2.0785	242	257	(R)(N)
1994.0322	1993.9772	27.5910	224	239	(R)(R)
2384.0542	2383.9524	42.6959	519	549	(R)(G)
2771.4476	2771.4288	6.8047	417	441	(K)(N)

The matched peptides cover 27% (178/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.3 Mean Error = 15.1058 ppm Data Tolerance = 47.6910 ppm Mean Missed Cleavages = 0.6