MS-Fit Search Results

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Sample ID (comment): HSGB04
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 1821 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.5e+012	29/61 (47%)	70898.7 / 5.38	HUMAN	HEAT SHOCK COGNATE 71 KDA PROTEIN
2	2.51e+012	28/61 (45%)	70871.6 / 5.38	MOUSE	HEAT SHOCK COGNATE 71 KDA PROTEIN
3	5.23e+004	12/61 (19%)	70021.5 / 5.56	HUMAN	HEAT SHOCK-RELATED 70 KDA PROTEIN 2 (HEAT SHOCK 70 KDA PROTEIN 2)
4	3.01e+004	11/61 (18%)	69741.4 / 5.58	MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2)
5	1.21e+003	9/61 (14%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)

Detailed Results

1. 29/61 matches (47%). 70898.7 Da, pI = 5.38. Acc. # P11142. HUMAN. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1081.5563	1081.5682	-11.0490	349	357	(K)(E)
1197.6879	1197.6632	20.6567	459	469	(K)(G)
1199.6941	1199.6748	16.0881	160	171	(K)(I)
1228.6475	1228.6286	15.3654	26	36	(K)(T)
1235.6501	1235.6247	20.5651	237	246	(R)(R)
1251.6340	1251.6196	11.5284	237	246	(R)MVNHFIAEFK(R)	1Met-ox
1253.6337	1253.6166	13.6262	302	311	(R)(G)
1303.6225	1303.5993	17.7951	540	550	(K)(A)
1319.6145	1319.5942	15.4184	540	550	(K)NSLESYAFNMK(A)	1Met-ox
1391.7368	1391.7258	7.9116	237	247	(R)(K)
1410.7178	1410.6687	34.7843	77	88	(R)(H)
1426.6899	1426.6637	18.3880	77	88	(R)RFDDAVVQSDMK(H)	1Met-ox
1480.7833	1480.7549	19.2207	300	311	(R)(G)
1487.7294	1487.7018	18.5352	37	49	(R)(L)
1616.8233	1616.7882	21.7225	113	126	(K)(M)
1632.8426	1632.7831	36.4697	113	126	(K)SFYPEEVSSMVLTK(M)	1Met-ox
1649.8258	1649.7957	18.2275	57	71	(K)(R)
1653.8713	1653.8324	23.5111	89	102	(K)(V)
1659.9100	1659.8958	8.6000	172	187	(R)(K)
1665.8427	1665.7906	31.2242	57	71	(K)NQVAMNPTNTVFDAK(R)	1Met-ox
1669.8610	1669.8273	20.1810	89	102	(K)HWPFMVVNDAGRPK(V)	1Met-ox
1691.7532	1691.7261	15.9923	221	236	(K)(M)
1745.8384	1745.8095	16.5997	584	597	(K)(E)
1788.0067	1787.9907	8.9173	172	188	(R)(V)
1788.0067	1787.9366	39.2005	570	583	(K)ILDKCNEIINWLDK(N)	1Cys-am
1982.0450	1981.9983	23.5441	138	155	(K)(Q)
1982.0450	1981.9653	40.2021	518	533	(R)(D)
2260.2065	2260.1461	26.6965	362	384	(K)(S)
2277.2076	2277.1338	32.4306	4	25	(K)GPAVGIDLGTTYSCVGVFQHGK(V)	1Cys-am
2758.3977	2758.4086	-3.9465	562	583	(K)INDEDKQKILDKCNEIINWLDK(N)	1Cys-am
2774.4332	2774.3273	38.1455	424	447	(K)(A)

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The matched peptides cover 49% (317/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.5 Mean Error = 19.8888 ppm Data Tolerance = 23.3724 ppm Mean Missed Cleavages = 0.4

2. 28/61 matches (45%). 70871.6 Da, pI = 5.38. Acc. # P08109. MOUSE. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5563 1081.5682 -11.0490 349 357 (K)(E)
1197.6879 1197.6632 20.6567 459 469 (K)(G)
1199.6941 1199.6748 16.0881 160 171 (K)(I)
1228.6475 1228.6286 15.3654 26 36 (K)(T)
1235.6501 1235.6247 20.5651 237 246 (R)(R)
1251.6340 1251.6196 11.5284 237 246 (R)MVNHFIAEFK(R) 1Met-ox
1253.6337 1253.6166 13.6262 302 311 (R)(G)
1303.6225 1303.5993 17.7951 540 550 (K)(A)
1319.6145 1319.5942 15.4184 540 550 (K)NSLESYAFNMK(A) 1Met-ox
1391.7368 1391.7258 7.9116 237 247 (R)(K)
1410.7178 1410.6687 34.7843 77 88 (R)(H)
1426.6899 1426.6637 18.3880 77 88 (R)RFDDAVVQSDMK(H) 1Met-ox
1480.7833 1480.7549 19.2207 300 311 (R)(G)
1487.7294 1487.7018 18.5352 37 49 (R)(L)
1616.8233 1616.7882 21.7225 113 126 (K)(M)
1632.8426 1632.7831 36.4697 113 126 (K)SFYPEEVSSMVLTK(M) 1Met-ox
1649.8258 1649.7957 18.2275 57 71 (K)(R)
1653.8713 1653.8324 23.5111 89 102 (K)(V)
1659.9100 1659.8958 8.6000 172 187 (R)(K)
1665.8427 1665.7906 31.2242 57 71 (K)NQVAMNPTNTVFDAK(R) 1Met-ox
1669.8610 1669.8273 20.1810 89 102 (K)HWPFMVVNDAGRPK(V) 1Met-ox
1691.7532 1691.7261 15.9923 221 236 (K)(M)
1745.8384 1745.8095 16.5997 584 597 (K)(E)
1788.0067 1787.9907 8.9173 172 188 (R)(V)
1982.0450 1981.9983 23.5441 138 155 (K)(Q)
1982.0450 1981.9653 40.2021 518 533 (R)(D)
2260.2065 2260.1461 26.6965 362 384 (K)(S)
2277.2076 2277.1338 32.4306 4 25 (K)GPAVGIDLGTTYSCVGVFQHGK(V) 1Cys-am
2774.4332 2774.3273 38.1455 424 447 (K)(A)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 45% (295/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 45.9 Mean Error = 20.0448 ppm Data Tolerance = 21.2308 ppm Mean Missed Cleavages = 0.3

3. 12/61 matches (19%). 70021.5 Da, pI = 5.56. Acc. # P54652. HUMAN. HEAT SHOCK-RELATED 70 KDA PROTEIN 2 (HEAT SHOCK 70 KDA PROTEIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5563 1081.5682 -11.0490 352 360 (K)(E)
1228.6475 1228.6286 15.3654 27 37 (K)(T)
1253.6337 1253.6166 13.6262 305 314 (R)(G)
1480.7833 1480.7549 19.2207 303 314 (R)(G)
1487.7294 1487.7018 18.5352 38 50 (R)(L)
1659.9100 1659.8958 8.6000 173 188 (R)(K)
1691.7532 1691.7261 15.9923 224 239 (K)(M)
1788.0067 1787.9907 8.9173 173 189 (R)(G)
1994.0730 1994.0493 11.8772 73 89 (K)(H)
2277.2076 2277.1338 32.4306 5 26 (R)(V)
2757.4707 2757.3444 45.8043 530 553 (K)(Q)
2758.3977 2758.4198 -8.0189 565 586 (K)ISEQDKNKILDKCQEVINWLDR(N) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1081.5563	1081.5682	-11.0490	352	360	(K)(E)
1228.6475	1228.6286	15.3654	27	37	(K)(T)
1253.6337	1253.6166	13.6262	305	314	(R)(G)
1480.7833	1480.7549	19.2207	303	314	(R)(G)
1487.7294	1487.7018	18.5352	38	50	(R)(L)
1659.9100	1659.8958	8.6000	173	188	(R)(K)
1691.7532	1691.7261	15.9923	224	239	(K)(M)
1788.0067	1787.9907	8.9173	173	189	(R)(G)
1994.0730	1994.0493	11.8772	73	89	(K)(H)
2277.2076	2277.1338	32.4306	5	26	(R)(V)
2757.4707	2757.3444	45.8043	530	553	(K)(Q)
2758.3977	2758.4198	-8.0189	565	586	(K)ISEQDKNKILDKCQEVINWLDR(N)	1Cys-am

The matched peptides cover 25% (163/639AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.7 Mean Error = 14.2751 ppm Data Tolerance = 30.6052 ppm Mean Missed Cleavages = 0.9

4. 11/61 matches (18%). 69741.4 Da, pI = 5.58. Acc. # P17156. MOUSE. HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5563 1081.5682 -11.0490 352 360 (K)(E)
1228.6475 1228.6286 15.3654 27 37 (K)(T)
1253.6337 1253.6166 13.6262 305 314 (R)(G)
1480.7833 1480.7549 19.2207 303 314 (R)(G)
1487.7294 1487.7018 18.5352 38 50 (R)(L)
1659.9100 1659.8958 8.6000 173 188 (R)(K)
1691.7532 1691.7261 15.9923 224 239 (K)(M)
1788.0067 1787.9907 8.9173 173 189 (R)(G)
1994.0730 1994.0493 11.8772 73 89 (K)(H)
2277.2076 2277.1338 32.4306 5 26 (R)(V)
2758.3977 2758.4198 -8.0189 565 586 (K)ISEQDKNKILDKCQEVINWLDR(N) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1081.5563	1081.5682	-11.0490	352	360	(K)(E)
1228.6475	1228.6286	15.3654	27	37	(K)(T)
1253.6337	1253.6166	13.6262	305	314	(R)(G)
1480.7833	1480.7549	19.2207	303	314	(R)(G)
1487.7294	1487.7018	18.5352	38	50	(R)(L)
1659.9100	1659.8958	8.6000	173	188	(R)(K)
1691.7532	1691.7261	15.9923	224	239	(K)(M)
1788.0067	1787.9907	8.9173	173	189	(R)(G)
1994.0730	1994.0493	11.8772	73	89	(K)(H)
2277.2076	2277.1338	32.4306	5	26	(R)(V)
2758.3977	2758.4198	-8.0189	565	586	(K)ISEQDKNKILDKCQEVINWLDR(N)	1Cys-am

The matched peptides cover 21% (139/633AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.0 Mean Error = 11.4088 ppm Data Tolerance = 24.4247 ppm Mean Missed Cleavages = 0.7

5. 9/61 matches (14%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3153 515.3306 -29.5968 404 407 (R)(L)
1277.7146 1277.7105 3.1961 473 483 (K)(T)
1357.6845 1357.6963 -8.7191 444 455 (K)(E)
1475.7991 1475.7858 8.9725 200 211 (R)(W)
1475.7991 1475.7494 33.6283 212 223 (K)(T)
1653.8713 1653.8158 33.5448 290 304 (K)(L)
1669.8610 1669.8107 30.1186 290 304 (K)DVDGAYMTKVDLQAK(L) 1Met-ox
1687.9162 1687.9707 -32.2756 66 82 (R)(G)
1994.0730 1993.9772 48.0461 224 239 (R)(R)
2277.2076 2277.1125 41.7903 9 30 (R)(T)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
515.3153	515.3306	-29.5968	404	407	(R)(L)
1277.7146	1277.7105	3.1961	473	483	(K)(T)
1357.6845	1357.6963	-8.7191	444	455	(K)(E)
1475.7991	1475.7858	8.9725	200	211	(R)(W)
1475.7991	1475.7494	33.6283	212	223	(K)(T)
1653.8713	1653.8158	33.5448	290	304	(K)(L)
1669.8610	1669.8107	30.1186	290	304	(K)DVDGAYMTKVDLQAK(L)	1Met-ox
1687.9162	1687.9707	-32.2756	66	82	(R)(G)
1994.0730	1993.9772	48.0461	224	239	(R)(R)
2277.2076	2277.1125	41.7903	9	30	(R)(T)

The matched peptides cover 18% (121/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.8 Mean Error = 12.8705 ppm Data Tolerance = 58.2944 ppm Mean Missed Cleavages = 0.5