MS-Fit Search Results

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Sample ID (comment): B8
Database searched: SwissProt.9.30.2001
Molecular weight search (1000 - 100000 Da) selects 94637 entries.
Full pI range: 100225 entries.
Species search ( HUMAN MOUSE ) selects 11762 entries.
Combined molecular weight, pI and species searches select 10474 entries.
Pre searches select 10474 entries.
MS-Fit search selects 1801 entries (results displayed for top 10 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.3e+007	23/74 (31%)	51335.1 / 5.41	HUMAN	KERATIN, TYPE II CYTOSKELETAL 7 (CYTOKERATIN 7) (K7) (CK 7)
2	9.24e+006	21/74 (28%)	53674.7 / 5.52	HUMAN	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8)
3	1.62e+005	16/74 (21%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
4	1.4e+005	14/74 (18%)	54449.6 / 5.50	MOUSE	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A)
5	3.92e+003	7/74 (9%)	61987.8 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)
6	3.36e+003	13/74 (17%)	59519.1 / 5.13	HUMAN	KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10)
7	2.97e+003	12/74 (16%)	82437.2 / 9.14	HUMAN	ZINC FINGER PROTEIN 225
8	2.93e+003	10/74 (13%)	89614.9 / 8.61	HUMAN	TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20)
9	961	8/74 (10%)	57842.4 / 5.01	MOUSE	KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (56 KDA CYTOKERATIN) (KERATIN, TYPE I CYTOSKELETAL 59 KDA)
10	831	9/74 (12%)	64511.6 / 6.12	HUMAN	KERATIN, TYPE II CYTOSKELETAL 3 (CYTOKERATIN 3) (K3) (CK3) (65 KDA CYTOKERATIN)

Detailed Results

1. 23/74 matches (31%). 51335.1 Da, pI = 5.41. Acc. # P08729. HUMAN. KERATIN, TYPE II CYTOSKELETAL 7 (CYTOKERATIN 7) (K7) (CK 7).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
827.4119	827.4303	-22.3327	102	108	(K)(V)
906.4349	906.4685	-37.0362	111	117	(R)(L)
1014.5100	1014.5220	-11.7842	78	86	(R)(V)
1043.5399	1043.5737	-32.4343	318	326	(R)(N)
1045.5328	1045.5682	-33.9062	123	130	(K)(S)
1060.5274	1060.5639	-34.3913	394	402	(R)(L)
1104.5638	1104.5802	-14.8227	53	64	(R)(E)
1196.5394	1196.5588	-16.1812	277	286	(R)(F)
1220.6304	1220.6527	-18.2559	188	198	(R)(K)
1242.6278	1242.6442	-13.2465	150	161	(R)(L)
1245.6059	1245.6439	-30.4705	87	96	(R)(A)
1272.6841	1272.7051	-16.4772	254	265	(R)(A)
1277.6976	1277.7105	-10.1560	383	393	(K)(K)
1336.6386	1336.6610	-16.7271	178	187	(K)(T)
1385.7136	1385.7389	-18.2223	352	363	(R)(A)
1385.7136	1385.7389	-18.2223	354	365	(K)(Q)
1406.7019	1406.7068	-3.5416	1	13	(-)SIHFSSPVFTSR(S)	Acet N
1418.7138	1418.7280	-9.9676	215	226	(K)(T)
1442.7931	1442.8007	-5.2879	137	149	(R)(G)
1453.8237	1453.8379	-9.7508	65	77	(R)(L)
1812.8856	1812.9053	-10.8794	199	214	(K)KDVDAAYMSKVELEAK(V)	1Met-ox
2125.0352	2125.0534	-8.5769	366	382	(K)(L)
3010.3790	3010.4527	-24.4648	227	253	(R)(S)
3026.4656	3026.4476	5.9565	227	253	(R)TLNETELTELQSQISDTSVVLSMDNSR(S)	1Met-ox

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The matched peptides cover 55% (262/469AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 31.1 Mean Error = -17.1325 ppm Data Tolerance = 21.4570 ppm Mean Missed Cleavages = 0.4

2. 21/74 matches (28%). 53674.7 Da, pI = 5.52. Acc. # P05787. HUMAN. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
827.4119 827.4303 -22.3327 102 108 (K)(V)
906.4349 906.4685 -37.0362 111 117 (R)(M)
1030.5436 1030.5686 -24.2542 123 130 (K)(T)
1045.5328 1045.5530 -19.3152 150 158 (R)(L)
1060.5274 1060.5639 -34.3913 393 401 (R)(L)
1079.5265 1079.5373 -9.9830 226 233 (R)(E)
1079.5265 1079.5122 13.3163 265 273 (K)(S)
1129.6011 1129.6217 -18.2055 342 352 (R)(L)
1129.6011 1129.6217 -18.2055 353 362 (K)(A)
1137.5341 1137.5792 -39.6701 286 295 (K)(H)
1169.5575 1169.5512 5.3837 276 285 (R)(Y)
1169.5575 1169.5512 5.3837 373 381 (R)EYQELMNVK(L) 1Met-ox
1277.6976 1277.7105 -10.1560 382 392 (K)(K)
1336.6386 1336.6670 -21.2578 253 264 (R)SLDMDSIIAEVK(A) 1Met-ox
1344.6625 1344.6759 -10.0260 329 341 (R)(G)
1419.7227 1419.7484 -18.0894 214 225 (R)(Q)
1428.6986 1428.6793 13.4771 274 285 (R)SRAEAESMYQIK(Y) 1Met-ox
1442.7931 1442.8331 -27.7178 149 160 (R)(L)
1459.7276 1459.6930 23.7236 19 32 (R)(I)
1475.7514 1475.6987 35.7534 402 414 (R)(T)
1879.7862 1879.7955 -4.9151 134 148 (R)SNMDNMFESYINNLR(R) 2Met-ox
2109.0222 2109.0134 4.1705 234 252 (R)(S)
2125.0352 2125.0083 12.6541 234 252 (R)ELQSQISDTSVVLSMDNSR(S) 1Met-ox
2510.1146 2510.2024 -34.9896 24 47 (R)(G)

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The matched peptides cover 48% (236/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 28.4 Mean Error = -9.8618 ppm Data Tolerance = 40.5435 ppm Mean Missed Cleavages = 0.3

3. 16/74 matches (21%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1014.5100 1014.4791 30.5135 1 8 (-)MSRQFSSR(S) 1Met-ox
1045.5328 1045.5754 -40.8023 178 185 (K)(S)
1157.5679 1157.5149 45.8568 464 472 (R)DYQELMNTK(L) 1Met-ox
1179.5815 1179.6010 -16.5013 377 386 (K)(H)
1265.6133 1265.6378 -19.2996 278 288 (R)(K)
1272.6841 1272.7388 -42.9552 176 185 (K)(S)
1277.6976 1277.6337 49.9497 247 257 (K)(N)
1277.6976 1277.7105 -10.1560 473 483 (K)(T)
1300.5809 1300.5302 38.9576 258 267 (K)(N)
1302.6466 1302.7018 -42.3524 393 403 (R)(V)
1357.7005 1357.6963 3.0869 444 455 (K)(E)
1383.6712 1383.6909 -14.1900 186 197 (K)(V)
1421.7352 1421.7389 -2.5971 277 288 (K)(K)
1475.7514 1475.7858 -23.3080 200 211 (R)(W)
1475.7514 1475.7494 1.3471 212 223 (K)(T)
1716.8281 1716.8517 -13.7148 418 432 (K)(G)
1993.9580 1993.9772 -9.6476 224 239 (R)(R)
2383.9365 2383.9524 -6.6675 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1014.5100	1014.4791	30.5135	1	8	(-)MSRQFSSR(S)	1Met-ox
1045.5328	1045.5754	-40.8023	178	185	(K)(S)
1157.5679	1157.5149	45.8568	464	472	(R)DYQELMNTK(L)	1Met-ox
1179.5815	1179.6010	-16.5013	377	386	(K)(H)
1265.6133	1265.6378	-19.2996	278	288	(R)(K)
1272.6841	1272.7388	-42.9552	176	185	(K)(S)
1277.6976	1277.6337	49.9497	247	257	(K)(N)
1277.6976	1277.7105	-10.1560	473	483	(K)(T)
1300.5809	1300.5302	38.9576	258	267	(K)(N)
1302.6466	1302.7018	-42.3524	393	403	(R)(V)
1357.7005	1357.6963	3.0869	444	455	(K)(E)
1383.6712	1383.6909	-14.1900	186	197	(K)(V)
1421.7352	1421.7389	-2.5971	277	288	(K)(K)
1475.7514	1475.7858	-23.3080	200	211	(R)(W)
1475.7514	1475.7494	1.3471	212	223	(K)(T)
1716.8281	1716.8517	-13.7148	418	432	(K)(G)
1993.9580	1993.9772	-9.6476	224	239	(R)(R)
2383.9365	2383.9524	-6.6675	519	549	(R)(G)

The matched peptides cover 31% (202/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.6 Mean Error = -4.0267 ppm Data Tolerance = 57.1781 ppm Mean Missed Cleavages = 0.5

4. 14/74 matches (18%). 54449.6 Da, pI = 5.50. Acc. # P11679. MOUSE. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
827.4119 827.4303 -22.3327 108 114 (K)(V)
906.4349 906.4685 -37.0362 117 123 (R)(M)
1030.5436 1030.5686 -24.2542 129 136 (K)(T)
1060.5274 1060.5639 -34.3913 398 406 (R)(L)
1079.5265 1079.5122 13.3163 271 279 (R)(S)
1169.5575 1169.5512 5.3837 378 386 (R)EYQELMNVK(L) 1Met-ox
1307.6688 1307.7211 -39.9581 387 397 (K)(K)
1344.6625 1344.6759 -10.0260 334 346 (R)(G)
1383.6712 1383.7272 -40.4849 103 114 (K)(V)
1419.7227 1419.7484 -18.0894 220 231 (R)(Q)
1475.7514 1475.6987 35.7534 407 419 (R)(T)
1879.7862 1879.7955 -4.9151 140 154 (R)SNMDNMFESYINNLR(R) 2Met-ox
2109.0222 2109.0134 4.1705 240 258 (R)(S)
2125.0352 2125.0083 12.6541 240 258 (R)ELQSQISDTSVVLSMDNSR(S) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
827.4119	827.4303	-22.3327	108	114	(K)(V)
906.4349	906.4685	-37.0362	117	123	(R)(M)
1030.5436	1030.5686	-24.2542	129	136	(K)(T)
1060.5274	1060.5639	-34.3913	398	406	(R)(L)
1079.5265	1079.5122	13.3163	271	279	(R)(S)
1169.5575	1169.5512	5.3837	378	386	(R)EYQELMNVK(L)	1Met-ox
1307.6688	1307.7211	-39.9581	387	397	(K)(K)
1344.6625	1344.6759	-10.0260	334	346	(R)(G)
1383.6712	1383.7272	-40.4849	103	114	(K)(V)
1419.7227	1419.7484	-18.0894	220	231	(R)(Q)
1475.7514	1475.6987	35.7534	407	419	(R)(T)
1879.7862	1879.7955	-4.9151	140	154	(R)SNMDNMFESYINNLR(R)	2Met-ox
2109.0222	2109.0134	4.1705	240	258	(R)(S)
2125.0352	2125.0083	12.6541	240	258	(R)ELQSQISDTSVVLSMDNSR(S)	1Met-ox

The matched peptides cover 28% (137/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.9 Mean Error = -11.4436 ppm Data Tolerance = 46.9297 ppm Mean Missed Cleavages = 0.1

5. 7/74 matches (9%). 61987.8 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1060.5274 1060.5639 -34.3913 224 232 (K)(M)
1157.5679 1157.5915 -20.3274 250 260 (R)(Q)
1307.6688 1307.6782 -7.1450 240 249 (R)(Q)
1837.9247 1837.9660 -22.4404 374 389 (R)(K)
2109.0222 2109.0538 -14.9890 427 443 (R)(M)
2510.1146 2510.1323 -7.0695 449 471 (K)(I)
2705.1827 2705.1617 7.7727 63 94 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1060.5274	1060.5639	-34.3913	224	232	(K)(M)
1157.5679	1157.5915	-20.3274	250	260	(R)(Q)
1307.6688	1307.6782	-7.1450	240	249	(R)(Q)
1837.9247	1837.9660	-22.4404	374	389	(R)(K)
2109.0222	2109.0538	-14.9890	427	443	(R)(M)
2510.1146	2510.1323	-7.0695	449	471	(K)(I)
2705.1827	2705.1617	7.7727	63	94	(R)(G)

The matched peptides cover 18% (118/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 9.5 Mean Error = -14.0843 ppm Data Tolerance = 27.0442 ppm Mean Missed Cleavages = 0.1

6. 13/74 matches (17%). 59519.1 Da, pI = 5.13. Acc. # P13645. HUMAN. KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1060.5274 1060.5097 16.6617 1 9 (-)MSVRYSSSK(H) 1Met-ox
1090.4986 1090.5315 -30.2038 148 156 (K)(L)
1109.4812 1109.4904 -8.2829 335 343 (K)(S)
1165.5924 1165.5853 6.1086 442 450 (R)(S)
1201.5897 1201.6177 -23.3249 246 256 (R)(R)
1234.6475 1234.6796 -25.9392 236 245 (R)(Q)
1300.5809 1300.5956 -11.3117 286 295 (K)(N)
1357.7005 1357.7188 -13.4606 246 257 (R)(V)
1365.6255 1365.6399 -10.5354 323 333 (R)(K)
1428.6986 1428.6905 5.6142 285 295 (K)(N)
1434.7426 1434.7705 -19.4232 440 450 (K)(S)
1493.7378 1493.7348 1.9450 323 334 (R)(D)
1707.7628 1707.7727 -5.7605 41 59 (K)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1060.5274	1060.5097	16.6617	1	9	(-)MSVRYSSSK(H)	1Met-ox
1090.4986	1090.5315	-30.2038	148	156	(K)(L)
1109.4812	1109.4904	-8.2829	335	343	(K)(S)
1165.5924	1165.5853	6.1086	442	450	(R)(S)
1201.5897	1201.6177	-23.3249	246	256	(R)(R)
1234.6475	1234.6796	-25.9392	236	245	(R)(Q)
1300.5809	1300.5956	-11.3117	286	295	(K)(N)
1357.7005	1357.7188	-13.4606	246	257	(R)(V)
1365.6255	1365.6399	-10.5354	323	333	(R)(K)
1428.6986	1428.6905	5.6142	285	295	(K)(N)
1434.7426	1434.7705	-19.4232	440	450	(K)(S)
1493.7378	1493.7348	1.9450	323	334	(R)(D)
1707.7628	1707.7727	-5.7605	41	59	(K)(G)

The matched peptides cover 17% (102/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.6 Mean Error = -9.0702 ppm Data Tolerance = 27.7434 ppm Mean Missed Cleavages = 0.6

7. 12/74 matches (16%). 82437.2 Da, pI = 9.14. Acc. # Q9UK10. HUMAN. ZINC FINGER PROTEIN 225.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1043.5399 1043.5274 11.9153 441 448 (R)(V)
1060.5274 1060.4998 25.9933 553 560 (K)(V)
1104.5638 1104.5148 44.3675 184 192 (K)(I)
1220.6304 1220.6612 -25.2586 296 305 (K)(H)
1234.6475 1234.6479 -0.2829 193 202 (R)(L)
1245.6059 1245.6632 -45.9468 353 361 (R)(H)
1300.5809 1300.5818 -0.7506 65 74 (K)(E)
1300.5809 1300.6030 -16.9964 318 328 (R)(S)
1406.7019 1406.7167 -10.5764 12 23 (K)(L)
1432.7624 1432.7735 -7.7317 253 264 (K)(C)
1434.7426 1434.7718 -20.3553 240 251 (K)(K)
1837.9247 1837.8399 46.1422 197 211 (R)(E)
2014.0746 2014.0405 16.9520 329 345 (K)(C)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1043.5399	1043.5274	11.9153	441	448	(R)(V)
1060.5274	1060.4998	25.9933	553	560	(K)(V)
1104.5638	1104.5148	44.3675	184	192	(K)(I)
1220.6304	1220.6612	-25.2586	296	305	(K)(H)
1234.6475	1234.6479	-0.2829	193	202	(R)(L)
1245.6059	1245.6632	-45.9468	353	361	(R)(H)
1300.5809	1300.5818	-0.7506	65	74	(K)(E)
1300.5809	1300.6030	-16.9964	318	328	(R)(S)
1406.7019	1406.7167	-10.5764	12	23	(K)(L)
1432.7624	1432.7735	-7.7317	253	264	(K)(C)
1434.7426	1434.7718	-20.3553	240	251	(K)(K)
1837.9247	1837.8399	46.1422	197	211	(R)(E)
2014.0746	2014.0405	16.9520	329	345	(K)(C)

The matched peptides cover 19% (137/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.2 Mean Error = 1.3440 ppm Data Tolerance = 54.1716 ppm Mean Missed Cleavages = 0.8

8. 10/74 matches (13%). 89614.9 Da, pI = 8.61. Acc. # P21580. HUMAN. TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5815 1179.5581 19.8767 677 685 (K)(F)
1201.5897 1201.5424 39.3210 764 774 (R)(C)
1220.6304 1220.6244 4.9338 719 728 (R)(S)
1406.7019 1406.7174 -11.0637 707 718 (R)(A)
1459.7276 1459.6891 26.3542 288 299 (K)(E)
1475.7514 1475.6841 45.6503 288 299 (K)VHFLTDPENEMK(E) 1Met-ox
1493.7378 1493.6921 30.5505 622 632 (K)(E)
1716.8281 1716.8267 0.8288 288 301 (K)(L)
1864.8164 1864.8726 -30.1471 622 635 (K)(H)
1993.9580 1994.0500 -46.1414 25 41 (R)(T)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.5815	1179.5581	19.8767	677	685	(K)(F)
1201.5897	1201.5424	39.3210	764	774	(R)(C)
1220.6304	1220.6244	4.9338	719	728	(R)(S)
1406.7019	1406.7174	-11.0637	707	718	(R)(A)
1459.7276	1459.6891	26.3542	288	299	(K)(E)
1475.7514	1475.6841	45.6503	288	299	(K)VHFLTDPENEMK(E)	1Met-ox
1493.7378	1493.6921	30.5505	622	632	(K)(E)
1716.8281	1716.8267	0.8288	288	301	(K)(L)
1864.8164	1864.8726	-30.1471	622	635	(K)(H)
1993.9580	1994.0500	-46.1414	25	41	(R)(T)

The matched peptides cover 11% (87/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.5 Mean Error = 8.0163 ppm Data Tolerance = 60.3536 ppm Mean Missed Cleavages = 0.4

9. 8/74 matches (10%). 57842.4 Da, pI = 5.01. Acc. # P02535. MOUSE. KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (56 KDA CYTOKERATIN) (KERATIN, TYPE I CYTOSKELETAL 59 KDA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
827.4119 827.3973 17.5682 155 161 (R)(V)
1090.4986 1090.5315 -30.2038 146 154 (R)(L)
1104.5638 1104.5247 35.4076 1 10 (-)MSVLYSSSSK(Q) 1Met-ox
1165.5924 1165.5853 6.1086 440 448 (R)(S)
1201.5897 1201.6177 -23.3249 244 254 (R)(R)
1357.7005 1357.7188 -13.4606 244 255 (R)(V)
1406.7019 1406.6512 36.0305 449 464 (R)(R)
2125.0352 2125.0375 -1.0752 265 282 (K)(K)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
827.4119	827.3973	17.5682	155	161	(R)(V)
1090.4986	1090.5315	-30.2038	146	154	(R)(L)
1104.5638	1104.5247	35.4076	1	10	(-)MSVLYSSSSK(Q)	1Met-ox
1165.5924	1165.5853	6.1086	440	448	(R)(S)
1201.5897	1201.6177	-23.3249	244	254	(R)(R)
1357.7005	1357.7188	-13.4606	244	255	(R)(V)
1406.7019	1406.6512	36.0305	449	464	(R)(R)
2125.0352	2125.0375	-1.0752	265	282	(K)(K)

The matched peptides cover 14% (81/570AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.8 Mean Error = 3.3813 ppm Data Tolerance = 50.3987 ppm Mean Missed Cleavages = 0.1

10. 9/74 matches (12%). 64511.6 Da, pI = 6.12. Acc. # P12035. HUMAN. KERATIN, TYPE II CYTOSKELETAL 3 (CYTOKERATIN 3) (K3) (CK3) (65 KDA CYTOKERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
827.4119 827.4303 -22.3327 209 215 (K)(V)
906.4349 906.4685 -37.0362 218 224 (R)(V)
1045.5328 1045.5642 -30.0589 397 406 (K)(H)
1104.5638 1104.6013 -33.9510 65 76 (K)(A)
1179.5815 1179.6010 -16.5013 260 269 (R)(G)
1383.6712 1383.6466 17.7889 278 288 (K)NMEDLVEDFKK(K) 1Met-ox
1418.7138 1418.7531 -27.6958 325 336 (K)(T)
1731.8970 1731.9757 -45.4525 216 229 (K)(W)
1838.9124 1838.9322 -10.7730 309 324 (K)(V)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
827.4119	827.4303	-22.3327	209	215	(K)(V)
906.4349	906.4685	-37.0362	218	224	(R)(V)
1045.5328	1045.5642	-30.0589	397	406	(K)(H)
1104.5638	1104.6013	-33.9510	65	76	(K)(A)
1179.5815	1179.6010	-16.5013	260	269	(R)(G)
1383.6712	1383.6466	17.7889	278	288	(K)NMEDLVEDFKK(K)	1Met-ox
1418.7138	1418.7531	-27.6958	325	336	(K)(T)
1731.8970	1731.9757	-45.4525	216	229	(K)(W)
1838.9124	1838.9322	-10.7730	309	324	(K)(V)

The matched peptides cover 14% (92/629AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.2 Mean Error = -22.8903 ppm Data Tolerance = 37.0725 ppm Mean Missed Cleavages = 0.6