MS-Fit Search Results

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Sample ID (comment): B10
Database searched: SwissProt.9.30.2001
Molecular weight search (1000 - 100000 Da) selects 94637 entries.
Full pI range: 100225 entries.
Species search ( HUMAN MOUSE ) selects 11762 entries.
Combined molecular weight, pI and species searches select 10474 entries.
Pre searches select 10474 entries.
MS-Fit search selects 1292 entries (results displayed for top 10 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	100.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.25e+006	12/43 (27%)	49229.9 / 5.89	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H (HNRNP H)
2	3.93e+004	10/43 (23%)	49264.0 / 5.89	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H' (HNRNP H') (FTP-3)
3	7.11e+003	8/43 (18%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
4	3.96e+003	10/43 (23%)	55993.5 / 8.76	HUMAN	SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (SERINE METHYLASE) (GLYCINE HYDROXYMETHYLTRANSFERASE) (SHMT)
5	792	4/43 (9%)	10992.2 / 9.30	HUMAN	SMALL INDUCIBLE CYTOKINE A1 PRECURSOR (T LYMPHOCYTE-SECRETED PROTEIN I-309)
6	608	9/43 (20%)	47244.7 / 9.52	HUMAN	FORKHEAD BOX PROTEIN P3 (ZINC FINGER PROTEIN JM2) (SCURFIN)
7	582	12/43 (27%)	48539.1 / 8.66	MOUSE	CARNITINE DEFICIENCY-ASSOCIATED PROTEIN EXPRESSED IN VENTRICLE 1 (CDV-1 PROTEIN)
8	522	10/43 (23%)	94506.3 / 5.65	HUMAN	OSMOTIC STRESS PROTEIN 94 (HEAT SHOCK 70-RELATED PROTEIN APG-1)
9	488	9/43 (20%)	47756.9 / 9.78	MOUSE	LUPUS LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG)
10	363	10/43 (23%)	89614.9 / 8.61	HUMAN	TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20)

Detailed Results

1. 12/43 matches (27%). 49229.9 Da, pI = 5.89. Acc. # P31943. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H (HNRNP H).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1518.7625	1518.7011	40.4157	17	29	(R)(F)
1615.7263	1615.6633	38.9555	263	275	(R)(Y)
1631.7434	1631.6583	52.1999	263	275	(R)DLNYCFSGMSDHR(Y)	1Met-ox
1684.8091	1684.7679	24.4065	99	114	(K)(L)
1841.9519	1841.8921	32.4432	151	167	(R)(A)
1997.0672	1996.9769	45.2410	300	316	(R)(V)
2001.0540	2001.0275	13.2410	200	217	(R)(G)
2107.0673	2106.9831	39.9367	50	68	(R)(L)
2112.0625	2111.9027	75.6416	276	294	(R)(G)
2142.1561	2142.0256	60.9288	356	375	(R)(Y)
2660.2318	2660.3783	-55.0767	50	73	(R)(D)
2717.2344	2717.0453	69.5931	234	259	(R)(F)

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The matched peptides cover 40% (183/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.9 Mean Error = 36.4939 ppm Data Tolerance = 67.8770 ppm Mean Missed Cleavages = 0.3

2. 10/43 matches (23%). 49264.0 Da, pI = 5.89. Acc. # P55795. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H' (HNRNP H') (FTP-3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.7349 1307.6306 79.7982 88 98 (K)(H)
1615.7263 1615.7824 -34.7283 1 14 (-)MMLSTEGREGFVVK(V) 2Met-ox
1615.7263 1615.6633 38.9555 263 275 (R)(Y)
1631.7434 1631.6583 52.1999 263 275 (R)DLNYCFSGMSDHR(Y) 1Met-ox
1684.8091 1684.7679 24.4065 99 114 (K)(L)
1841.9519 1841.8921 32.4432 151 167 (R)(A)
2001.0540 2001.0275 13.2410 200 217 (R)(G)
2029.0806 2028.9489 64.8969 300 316 (R)(V)
2044.9804 2044.9439 17.8611 300 316 (R)ATENDIYNFFSPLNPMR(V) 1Met-ox
2121.1116 2120.9988 53.1867 50 68 (R)(L)
2660.2318 2660.0238 78.1750 234 259 (R)(F)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.7349	1307.6306	79.7982	88	98	(K)(H)
1615.7263	1615.7824	-34.7283	1	14	(-)MMLSTEGREGFVVK(V)	2Met-ox
1615.7263	1615.6633	38.9555	263	275	(R)(Y)
1631.7434	1631.6583	52.1999	263	275	(R)DLNYCFSGMSDHR(Y)	1Met-ox
1684.8091	1684.7679	24.4065	99	114	(K)(L)
1841.9519	1841.8921	32.4432	151	167	(R)(A)
2001.0540	2001.0275	13.2410	200	217	(R)(G)
2029.0806	2028.9489	64.8969	300	316	(R)(V)
2044.9804	2044.9439	17.8611	300	316	(R)ATENDIYNFFSPLNPMR(V)	1Met-ox
2121.1116	2120.9988	53.1867	50	68	(R)(L)
2660.2318	2660.0238	78.1750	234	259	(R)(F)

The matched peptides cover 33% (151/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.3 Mean Error = 38.2214 ppm Data Tolerance = 66.4556 ppm Mean Missed Cleavages = 0.2

3. 8/43 matches (18%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
704.3287 704.3830 -77.2069 252 257 (R)(N)
1179.6142 1179.6010 11.2005 377 386 (K)(H)
1277.7753 1277.7105 50.6779 473 483 (K)(T)
1475.7947 1475.7858 6.0005 200 211 (R)(W)
1475.7947 1475.7494 30.6562 212 223 (K)(T)
1716.9243 1716.8517 42.2871 418 432 (K)(G)
1994.0935 1993.9772 58.3356 224 239 (R)(R)
2121.1116 2121.0114 47.2596 9 29 (R)(R)
2384.0775 2383.9524 52.4763 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
704.3287	704.3830	-77.2069	252	257	(R)(N)
1179.6142	1179.6010	11.2005	377	386	(K)(H)
1277.7753	1277.7105	50.6779	473	483	(K)(T)
1475.7947	1475.7858	6.0005	200	211	(R)(W)
1475.7947	1475.7494	30.6562	212	223	(K)(T)
1716.9243	1716.8517	42.2871	418	432	(K)(G)
1994.0935	1993.9772	58.3356	224	239	(R)(R)
2121.1116	2121.0114	47.2596	9	29	(R)(R)
2384.0775	2383.9524	52.4763	519	549	(R)(G)

The matched peptides cover 20% (134/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.6 Mean Error = 24.6319 ppm Data Tolerance = 84.7004 ppm Mean Missed Cleavages = 0.1

4. 10/43 matches (23%). 55993.5 Da, pI = 8.76. Acc. # P34897. HUMAN. SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (SERINE METHYLASE) (GLYCINE HYDROXYMETHYLTRANSFERASE) (SHMT).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1135.6332 1135.6475 -12.6308 220 230 (R)(L)
1169.5667 1169.5479 16.0765 306 314 (R)(I)
1179.6142 1179.5502 54.2455 237 245 (R)(A)
1509.7715 1509.7372 22.7590 70 82 (R)(A)
1509.7715 1509.8654 -62.1859 281 293 (K)(K)
1548.8805 1548.8386 27.0645 201 214 (K)(L)
1878.0556 1878.0310 13.1142 270 286 (K)(S)
1994.0935 1993.9983 47.7385 298 314 (K)(I)
2001.0540 2001.1133 -29.6464 197 214 (K)(L)
2001.0540 2000.9938 30.0829 341 356 (K)QACTPMFREYSLQVLK(N) 1Met-ox
2001.0540 2001.0439 5.0346 399 416 (R)(S)
2028.0761 2027.9748 49.9341 105 121 (R)(R)
2112.0625 2112.1777 -54.5465 390 409 (K)(N)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1135.6332	1135.6475	-12.6308	220	230	(R)(L)
1169.5667	1169.5479	16.0765	306	314	(R)(I)
1179.6142	1179.5502	54.2455	237	245	(R)(A)
1509.7715	1509.7372	22.7590	70	82	(R)(A)
1509.7715	1509.8654	-62.1859	281	293	(K)(K)
1548.8805	1548.8386	27.0645	201	214	(K)(L)
1878.0556	1878.0310	13.1142	270	286	(K)(S)
1994.0935	1993.9983	47.7385	298	314	(K)(I)
2001.0540	2001.1133	-29.6464	197	214	(K)(L)
2001.0540	2000.9938	30.0829	341	356	(K)QACTPMFREYSLQVLK(N)	1Met-ox
2001.0540	2001.0439	5.0346	399	416	(R)(S)
2028.0761	2027.9748	49.9341	105	121	(R)(R)
2112.0625	2112.1777	-54.5465	390	409	(K)(N)

The matched peptides cover 30% (152/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.3 Mean Error = 8.2339 ppm Data Tolerance = 75.9949 ppm Mean Missed Cleavages = 0.9

5. 4/43 matches (9%). 10992.2 Da, pI = 9.30. Acc. # P22362. HUMAN. SMALL INDUCIBLE CYTOKINE A1 PRECURSOR (T LYMPHOCYTE-SECRETED PROTEIN I-309).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1518.7625 1518.7375 16.4570 71 83 (K)(H)
1684.8091 1684.7827 15.6294 33 45 (R)(A)
1997.0672 1997.0139 26.6769 69 85 (R)(K)
2718.2030 2718.3920 -69.5385 1 24 (-)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1518.7625	1518.7375	16.4570	71	83	(K)(H)
1684.8091	1684.7827	15.6294	33	45	(R)(A)
1997.0672	1997.0139	26.6769	69	85	(R)(K)
2718.2030	2718.3920	-69.5385	1	24	(-)(S)

The matched peptides cover 56% (54/96AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 9.3 Mean Error = -2.6938 ppm Data Tolerance = 89.6910 ppm Mean Missed Cleavages = 0.5

6. 9/43 matches (20%). 47244.7 Da, pI = 9.52. Acc. # Q9BZS1. HUMAN. FORKHEAD BOX PROTEIN P3 (ZINC FINGER PROTEIN JM2) (SCURFIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1169.5667 1169.6642 -83.4127 28 39 (R)(G)
1307.7349 1307.6973 28.7770 376 386 (R)(H)
1863.9956 1863.9692 14.1674 333 347 (K)(W)
1994.0935 1993.9871 53.3724 398 414 (R)(K)
1997.0672 1997.0391 14.0819 387 402 (R)(G)
2019.0426 2019.1140 -35.3484 145 163 (K)(E)
2027.0261 2027.0786 -25.9420 28 48 (R)(D)
2107.0673 2107.0812 -6.6203 370 386 (R)(H)
2563.3998 2563.2821 45.8938 357 375 (K)(N)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1169.5667	1169.6642	-83.4127	28	39	(R)(G)
1307.7349	1307.6973	28.7770	376	386	(R)(H)
1863.9956	1863.9692	14.1674	333	347	(K)(W)
1994.0935	1993.9871	53.3724	398	414	(R)(K)
1997.0672	1997.0391	14.0819	387	402	(R)(G)
2019.0426	2019.1140	-35.3484	145	163	(K)(E)
2027.0261	2027.0786	-25.9420	28	48	(R)(D)
2107.0673	2107.0812	-6.6203	370	386	(R)(H)
2563.3998	2563.2821	45.8938	357	375	(K)(N)

The matched peptides cover 26% (113/431AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.9 Mean Error = 0.5521 ppm Data Tolerance = 86.9001 ppm Mean Missed Cleavages = 1.2

7. 12/43 matches (27%). 48539.1 Da, pI = 8.66. Acc. # O35594. MOUSE. CARNITINE DEFICIENCY-ASSOCIATED PROTEIN EXPRESSED IN VENTRICLE 1 (CDV-1 PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6142 1179.5390 63.7696 379 387 (R)(K)
1307.7349 1307.6339 77.2202 379 388 (R)(Q)
1509.7715 1509.7405 20.5263 218 230 (K)(L)
1548.8805 1548.8056 48.3813 206 219 (R)(T)
1631.7434 1631.7553 -7.2819 192 205 (K)(V)
1684.8091 1684.7497 35.2259 376 387 (K)MWRDLEQLMECK(K) 2Met-ox
1780.9591 1780.8548 58.5163 376 388 (K)(Q)
1997.0672 1997.0312 18.0191 68 83 (K)KMMRNEPIEGKLSLYR(Q) 2Met-ox
2028.0761 2028.1395 -31.2468 152 168 (K)(T)
2121.1116 2121.0069 49.3774 251 266 (R)(K)
2142.1561 2142.0250 61.2332 318 335 (R)(K)
2717.2344 2717.3681 -49.2047 388 411 (K)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.6142	1179.5390	63.7696	379	387	(R)(K)
1307.7349	1307.6339	77.2202	379	388	(R)(Q)
1509.7715	1509.7405	20.5263	218	230	(K)(L)
1548.8805	1548.8056	48.3813	206	219	(R)(T)
1631.7434	1631.7553	-7.2819	192	205	(K)(V)
1684.8091	1684.7497	35.2259	376	387	(K)MWRDLEQLMECK(K)	2Met-ox
1780.9591	1780.8548	58.5163	376	388	(K)(Q)
1997.0672	1997.0312	18.0191	68	83	(K)KMMRNEPIEGKLSLYR(Q)	2Met-ox
2028.0761	2028.1395	-31.2468	152	168	(K)(T)
2121.1116	2121.0069	49.3774	251	266	(R)(K)
2142.1561	2142.0250	61.2332	318	335	(R)(K)
2717.2344	2717.3681	-49.2047	388	411	(K)(L)

The matched peptides cover 34% (142/414AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.9 Mean Error = 28.7113 ppm Data Tolerance = 79.8347 ppm Mean Missed Cleavages = 1.7

8. 10/43 matches (23%). 94506.3 Da, pI = 5.65. Acc. # O95757. HUMAN. OSMOTIC STRESS PROTEIN 94 (HEAT SHOCK 70-RELATED PROTEIN APG-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1135.6332 1135.5855 41.9717 607 615 (K)(E)
1307.7349 1307.6959 29.7998 186 196 (K)(N)
1475.7947 1475.7342 40.9946 361 374 (K)(G)
1841.9519 1842.0350 -45.1131 54 69 (K)(L)
2001.0540 2001.0955 -20.7722 85 102 (R)(V)
2027.0261 2026.9722 26.5838 459 477 (R)(V)
2112.0625 2112.0986 -17.0914 1 19 (-)(S)
2112.0625 2111.9676 44.9056 740 757 (K)(L)
2121.1116 2121.1490 -17.6514 709 725 (K)IQLVMKVIEAYRNKDER(Y) 1Met-ox
2144.1041 2143.9575 68.3857 740 757 (K)CISDAMSWLNSKMNAQNK(L) 2Met-ox
2164.1961 2164.1548 19.0402 258 274 (K)(K)
2164.1961 2164.0320 75.8299 684 700 (K)(A)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1135.6332	1135.5855	41.9717	607	615	(K)(E)
1307.7349	1307.6959	29.7998	186	196	(K)(N)
1475.7947	1475.7342	40.9946	361	374	(K)(G)
1841.9519	1842.0350	-45.1131	54	69	(K)(L)
2001.0540	2001.0955	-20.7722	85	102	(R)(V)
2027.0261	2026.9722	26.5838	459	477	(R)(V)
2112.0625	2112.0986	-17.0914	1	19	(-)(S)
2112.0625	2111.9676	44.9056	740	757	(K)(L)
2121.1116	2121.1490	-17.6514	709	725	(K)IQLVMKVIEAYRNKDER(Y)	1Met-ox
2144.1041	2143.9575	68.3857	740	757	(K)CISDAMSWLNSKMNAQNK(L)	2Met-ox
2164.1961	2164.1548	19.0402	258	274	(K)(K)
2164.1961	2164.0320	75.8299	684	700	(K)(A)

The matched peptides cover 20% (175/839AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.3 Mean Error = 20.5736 ppm Data Tolerance = 75.8798 ppm Mean Missed Cleavages = 1.2

9. 9/43 matches (20%). 47756.9 Da, pI = 9.78. Acc. # P32067. MOUSE. LUPUS LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1548.8805 1548.8637 10.8250 61 74 (R)(S)
1878.0556 1877.8755 95.8886 370 384 (R)(R)
2028.0761 2028.0640 5.9581 330 349 (K)(R)
2029.0806 2029.0680 6.2196 38 54 (K)(F)
2044.9804 2045.0629 -40.3546 38 54 (K)EQIKLDEGWVPLETMIK(F) 1Met-ox
2107.0673 2107.1704 -48.9479 260 277 (K)(A)
2121.1116 2121.1272 -7.3670 175 191 (K)(K)
2660.2318 2660.4373 -77.2655 33 54 (R)(F)
2717.2344 2717.3550 -44.3900 17 37 (K)(E)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1548.8805	1548.8637	10.8250	61	74	(R)(S)
1878.0556	1877.8755	95.8886	370	384	(R)(R)
2028.0761	2028.0640	5.9581	330	349	(K)(R)
2029.0806	2029.0680	6.2196	38	54	(K)(F)
2044.9804	2045.0629	-40.3546	38	54	(K)EQIKLDEGWVPLETMIK(F)	1Met-ox
2107.0673	2107.1704	-48.9479	260	277	(K)(A)
2121.1116	2121.1272	-7.3670	175	191	(K)(K)
2660.2318	2660.4373	-77.2655	33	54	(R)(F)
2717.2344	2717.3550	-44.3900	17	37	(K)(E)

The matched peptides cover 29% (122/415AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.9 Mean Error = -11.0482 ppm Data Tolerance = 100.7634 ppm Mean Missed Cleavages = 2.0

10. 10/43 matches (23%). 89614.9 Da, pI = 8.61. Acc. # P21580. HUMAN. TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1135.6332 1135.5860 41.5587 279 287 (R)(V)
1179.6142 1179.5581 47.5795 677 685 (K)(F)
1475.7947 1475.6841 74.9607 288 299 (K)VHFLTDPENEMK(E) 1Met-ox
1509.7715 1509.8277 -37.2025 124 136 (R)(N)
1518.7625 1518.7197 28.1503 531 542 (R)(T)
1716.9243 1716.8267 56.8315 288 301 (K)(L)
1863.9956 1863.9461 26.5707 1 16 (-)(K)
1994.0935 1994.0500 21.8394 25 41 (R)(T)
2027.0261 2026.9881 18.7189 743 761 (R)(C)
2704.2160 2704.3378 -45.0447 677 697 (K)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1135.6332	1135.5860	41.5587	279	287	(R)(V)
1179.6142	1179.5581	47.5795	677	685	(K)(F)
1475.7947	1475.6841	74.9607	288	299	(K)VHFLTDPENEMK(E)	1Met-ox
1509.7715	1509.8277	-37.2025	124	136	(R)(N)
1518.7625	1518.7197	28.1503	531	542	(R)(T)
1716.9243	1716.8267	56.8315	288	301	(K)(L)
1863.9956	1863.9461	26.5707	1	16	(-)(K)
1994.0935	1994.0500	21.8394	25	41	(R)(T)
2027.0261	2026.9881	18.7189	743	761	(R)(C)
2704.2160	2704.3378	-45.0447	677	697	(K)(S)

The matched peptides cover 15% (121/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.3 Mean Error = 23.3962 ppm Data Tolerance = 76.3177 ppm Mean Missed Cleavages = 1.1