MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B19
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2426 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/91(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.095e+00612 (13)25.013.2-40.1124 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
28.923e+00513 (14)20.014.3-71.3104 97287/6.7MOUSEGlycogen phosphorylase, muscle form (Myophosphorylase)
34.464e+00510 (10)14.011.0-56.3127 83157/8.7MOUSERibosomal protein S6 kinase alpha 2 (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2) (p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3)
41.966e+00512 (13)13.013.2-49.3110 83254/8.8HUMANRibosomal protein S6 kinase alpha 2 (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2) (p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3)
57.941e+0047 (7)16.07.7-21.2169 73103/6.1MOUSEKininogen precursor [Contains: Bradykinin]
65.731e+0049 (9)14.09.9-75.9102 97093/6.6HUMANGlycogen phosphorylase, muscle form (Myophosphorylase)
75.265e+0049 (9)22.09.9-62.1128 54886/9.8MOUSEGrowth/differentiation factor 5 precursor (GDF-5)
85.072e+00414 (15)16.015.4-27.0161 82437/9.1HUMANZinc finger protein 225
94.550e+0049 (9)25.09.9-52.6181 43136/6.3HUMANMitogen-activated protein kinase 3 (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERK1) (ERT2) (p44-MAPK) (Microtubule-associated protein-2 kinase)
104.008e+0049 (9)12.09.9-40.6152 96099/4.6HUMANVery low-density lipoprotein receptor precursor (VLDL receptor)
45313..................xx....x......x.............x.....xx.....x....xx.........x............x...
18650.x.........................x....................xx........x.x...xx..x....xx.....x.x........
40308...........................x................x.x..xx...xx..x.................x......x.......
51914....x.x.....................................x.x..xxx..xx..x....x............x..............
85222.............................x..x...........x............................x...x.......x....x
54458.x.........................x....................x............x...x..x.....x.....x.x........
6696x....xx...x....................................x................x................x....x.x..
74665...xx...x...................xx.......x.....x.x..x..x...x..x.x....x.........................
87184....xx..xx.....................................x...x...x...........x......................x
75625............x.x.....x..........x...............x.........x..x........................xx....


Detailed Results

1. 12/91 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1015.38611015.4406-541Met-ox 2902980(K)DVDGAYMTK(V)
1016.38781016.5013-1123563640(K) (S)
1033.38201033.5166-1304844920(R) (M)
1066.44441066.5169-682702771(K) (T)
1179.56671179.6010-293773860(K) (H)
1300.54941300.5302152582670(K) (N)
1302.57031302.7018-1013934031(R) (V)
1302.57031302.7157-1123443550(R) (A)
1332.65371332.52001002Met-ox 2582670(K)NMQDMVEDYR(N)
1383.67021383.6909-151861970(K) (V)
1475.74001475.7494-6.42122230(K) (T)
1475.74001475.7858-312002110(R) (W)
1993.95091993.9772-132242390(R) (R)
2383.93712383.9524-6.45195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (161/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/91 matches (14%).
Acc. #: Species: MOUSE Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97287 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
947.3487947.4733-1312362430(R) (L)
1054.41821054.4846-633253330(R) (V)
1234.62421234.6465-181Met-ox 4294391(R)MSLVEEGAVKR(I)
1234.62421234.6465-181Met-ox 4284381(R)RMSLVEEGAVK(R)
1283.57491283.6306-437847931(K) (N)
1332.65371332.6694-121111(-) (K)
1343.64961343.7535-7718301(R) (N)
1475.74001475.7535-9.13603711(R) (T)
1566.75211566.7916-252572700(K) (N)
1765.72391765.9489-1272963100(K) (R)
1765.72391765.9713-1405565701(K) (I)
1940.89911941.0856-965765901(K) (I)
1993.95091994.0248-3745611(R) (D)
2117.83312118.1381-1443343520(K) (I)
2133.86132134.1330-1271Met-ox 3343520(K)VAIQLNDTHPSLAIPELMR(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (170/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/91 matches (10%).
Acc. #: Species: MOUSE Name: Ribosomal protein S6 kinase alpha 2 (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2) (p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3)
Index: MW: 83157 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1054.41821054.5533-12840480(K) (E)
1165.59721165.6985-871341420(K) (G)
1193.54551193.5955-421241330(K) (L)
1283.57491283.7112-1064634730(R) (D)
1285.62231285.6363-116937040(K) (T)
1313.53521313.6953-1224524620(R) (Y)
1316.76931316.6747724814900(K) (G)
1332.65371332.6696-121Met-ox 4814900(K)YVYLVMELMR(G)
2057.00872057.0564-234814971(K) (I)
2057.00872057.0780-346766921(R) (G)
2139.83352140.1052-1276696851(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (103/733AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/91 matches (13%).
Acc. #: Species: HUMAN Name: Ribosomal protein S6 kinase alpha 2 (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2) (p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3)
Index: MW: 83254 Da pI: 8.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
957.3660957.4504-885525590(R) (Q)
968.4058968.4590-55pyroGlu 5015071(R)QRYFSER(E)
1165.59721165.6985-871341420(K) (G)
1193.54551193.5955-421241330(K) (L)
1283.57491283.7112-1064634730(R) (D)
1285.62231285.6363-116937040(K) (T)
1300.54941300.6798-1004814900(K) (G)
1313.53521313.6953-1224524620(R) (Y)
1316.76931316.6747721Met-ox 4814900(K)FVYLVMELMR(G)
1332.65371332.6696-122Met-ox 4814900(K)FVYLVMELMR(G)
1383.67021383.6942-171Met-ox 79911(K)VKGSDAGQLYAMK(V)
2057.00872057.0564-231Met-ox 4814971(K)FVYLVMELMRGGELLDR(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (98/733AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/91 matches (7%).
Acc. #: Species: MOUSE Name: Kininogen precursor [Contains: Bradykinin]
Index: MW: 73103 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1060.43501060.5692-1273803880(R) (S)
1068.48091068.5689-825615700(R) (A)
1165.59721165.5676252302390(R) (I)
1940.89911940.8581213273421(K) (H)
2100.91672100.87552080970(K) (D)
2244.20992243.98141022102290(R) (G)
2717.04032717.3319-1071371600(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (107/661AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/91 matches (9%).
Acc. #: Species: HUMAN Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97093 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
947.3487947.4733-1312362430(R) (L)
1054.41821054.4846-633253330(R) (V)
1234.62421234.6465-181Met-ox 4294391(R)MSLVEEGAVKR(I)
1234.62421234.6465-181Met-ox 4284381(R)RMSLVEEGAVK(R)
1351.61881351.7560-1025986091(K) (A)
1566.75211566.7916-252572700(K) (N)
1566.75211566.8168-417417540(R) (Q)
1765.72391765.9489-1272963100(K) (R)
1993.95091994.0248-3745611(R) (D)
2117.83312118.1381-1443343520(K) (I)
2133.86132134.1330-1271Met-ox 3343520(K)VAIQLNDTHPSLAIPELMR(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (120/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/91 matches (9%).
Acc. #: Species: MOUSE Name: Growth/differentiation factor 5 precursor (GDF-5)
Index: MW: 54886 Da pI: 9.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
943.5602943.5213412262340(K) (I)
965.5384965.5420-3.7pyroGlu 2672750(R)QPAALLDVR(S)
968.4058968.5278-1263263331(R) (A)
982.4608982.5686-1102672750(R) (S)
1232.58491232.6751-733153251(R) (T)
1475.74001475.709021951090(R) (Q)
2121.84052122.0391-942963121(R) (G)
2342.93382343.1960-1121541720(K) (T)
2705.17202705.4501-10349731(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (113/495AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 14/91 matches (15%).
Acc. #: Species: HUMAN Name: Zinc finger protein 225
Index: MW: 82437 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
952.3549952.3981-456826881(K) (Y)
957.3660957.4940-1343363431(R) (Y)
972.3776972.4539-784084150(K) (Y)
1058.42501058.5635-1316176250(R) (C)
1060.43501060.4998-615535600(K) (V)
1101.31441101.4709-1423443511(R) (R)
1158.52961158.4924324304381(R) (R)
1179.56671179.6625-81AcetN 1111(-)TTLKEAVTFK(D)
1234.62421234.6479-191932021(R) (L)
1300.54941300.5818-2565740(K) (E)
1300.54941300.6030-413183281(R) (S)
1316.76931316.57681461Met-ox 65740(K)FWMMETATQR(E)
1316.76931316.7075473243351(K) (M)
1332.65371332.5717622Met-ox 65740(K)FWMMETATQR(E)
1343.64961343.5724575425521(K) (F)
1566.75211566.7811-193293421(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (117/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/91 matches (9%).
Acc. #: Species: HUMAN Name: Mitogen-activated protein kinase 3 (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERK1) (ERT2) (p44-MAPK) (Microtubule-associated protein-2 kinase)
Index: MW: 43136 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
957.3660957.5046-1453713790(R) (-)
965.5384965.4879521821890(K) (I)
972.3776972.4903-11633410(K) (Y)
980.3924980.4875-971Met-ox 3103170(R)MLTFNPNK(R)
1232.58491232.6487-522993091(K) (M)
1300.54941300.6068-441150(-) (R)
1316.76931316.60171271Met-ox 1150(-)MAAAAAQGGGGGEPR(R)
1707.72381707.8277-6172841(K) (T)
2717.04032717.4159-1382252480(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (97/379AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 9/91 matches (9%).
Acc. #: Species: HUMAN Name: Very low-density lipoprotein receptor precursor (VLDL receptor)
Index: MW: 96099 Da pI: 4.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
989.3840989.4580-753463530(R) (E)
994.4238994.3610632582650(R) (D)
1022.37281022.4828-1083283360(R) (V)
1066.44441066.5421-924925010(K) (V)
1232.58491232.6751-734404491(K) (D)
1329.64891329.7014-404704820(R) (L)
1343.64961343.6306141Met-ox 8298390(K)SMNFDNPVYLK(T)
2244.20992244.0317793733910(K) (K)
2342.93382343.2510-1351200(-) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (108/873AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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