MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.2.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B21
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113997
Molecular weight search (1000 - 100000 Da) selects 107696 entries.
Full pI range: 113997 entries.
Species search ( HUMAN MOUSE ) selects 13846 entries.
Combined molecular weight, pI and species searches select 12267 entries.
Pre searches select 12267 entries.

Data Set 1 Results

MS-Fit search selects 3315 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/116(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
18.947e+02343 (37)55.037.127.033.5 89323/5.1HUMANTransitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)-ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]
22.389e+02343 (37)52.037.126.131.4 89309/5.1MOUSETRANSITIONAL ENDOPLASMIC RETICULUM ATPASE (TER ATPASE) (15S MG(2+)-ATPASE P97 SUBUNIT) (VALOSIN CONTAINING PROTEIN) (VCP) [CONTAINS: VALOSIN]
31.161e+00611 (9)21.09.515.553.4 72979/8.8HUMANZinc finger protein 189
43.637e+00512 (10)22.010.35.7484.7 69355/6.2MOUSEPhosphoenolpyruvate carboxykinase, cytosolic [GTP] (Phosphoenolpyruvate carboxylase) (PEPCK-C)
53.468e+00515 (12)23.012.914.479.5 85593/9.3HUMANHypothetical zinc finger protein KIAA1349
108359xx..x.xx..xxxx...x..x..x....x.x..x.x.......x..x........xx.....x.xx.x.x.x...xx..x.....x.xx.....xxx.xx......xx...xxx.x
88422xx..x.xx..xxxx...x..x..x....x.x..x.x.......x..x........xx.....x.xx.x.x.x...xx..x.....x.xx.....xxx.xx......xx...xxx.x
42914........x..........x...........x....x................x...............x....x...xx..............................x...x.
83172....................x.............x....................x...x....................xx..x........xx......x.......x..x...
238..........xxx.....x.x.......x.................x..x....x..x.........x...............xx.........x..................x..


Detailed Results

1. 43/116 matches (37%).
Acc. #: Species: HUMAN Name: Transitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)-ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]
Index: MW: 89323 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
947.6030947.5678371481551(R) (G)
962.5244962.4795477017080(R) (R)
986.5523986.5345182182250(K) (H)
1002.54291002.5294131Met-ox 2182250(K)EMVELPLR(H)
1034.49521034.4366577347410(R) (F)
1049.57671049.5519246696770(K) (M)
1051.56821051.52464146530(K) (G)
1067.54831067.5196271Met-ox 46530(K)MDELQLFR(G)
1075.56341075.5384232782870(K) (K)
1094.66841094.6574103783860(R) (N)
1118.61171118.5806287017091(R) (E)
1172.69271172.6679212402510(R) (T)
1203.64811203.6333122782881(K) (A)
1242.68491242.6330423663770(R) (L)
1265.67961265.6353355135240(K) (T)
1329.74171329.6915384544650(R) (E)
1430.84891430.8293143243360(R) (Q)
1446.86051446.8242251Met-ox 3243360(R)IVSQLLTLMDGLK(Q)
1554.87751554.820237961090(R) (Y)
1556.87871556.8576146396510(R) (S)
1592.86611592.8107355305430(K) (G)
1602.95051602.9253161Met-ox 3233361(R)RIVSQLLTLMDGLK(Q)
1629.85221629.8059287547661(R) (G)
1645.85321645.8008321Met-ox 7547661(R)KYEMFAQTLQQSR(G)
1660.89411660.8295393633771(R) (L)
1695.94751695.8297691Met-ox 6006140(R)VINQILTEMDGMSTK(K)
1695.94751695.9256131Met-ox 2182311(K)EMVELPLRHPALFK(A)
1799.93801799.9907-296396531(R) (V)
1811.02061810.9955142963120(K) (R)
1823.98241823.9180354885020(R) (F)
1823.98241823.9247321Met-ox 6006151(R)VINQILTEMDGMSTKK(N)
1935.99811935.9275365445600(K) (E)
1938.00711937.9683205685840(R) (A)
1951.97741951.9224281Met-ox 5445600(K)GPELLTMWFGESEANVR(E)
2138.06232138.01092466831(R) (M)
2146.19752146.1555203393580(R) (R)
2155.05322154.950148pyroGlu 1Met-ox 7147320(R)QTNPSAMEVEEDDPVPEIR(R)
2162.21862162.1504321Met-ox 3393580(R)AHVIVMAATNRPNSIDPALR(R)
2170.17092170.12432126450(R) (M)
2256.16302256.1375112572770(R) (L)
2272.14692272.13246.41Met-ox 2572770(R)AVANETGAFFFLINGPEIMSK(L)
2318.28892318.2515161Met-ox 3393591(R)AHVIVMAATNRPNSIDPALRR(F)
2318.28892318.27207.33663861(R) (N)
2328.15582327.9938701922111(R) (Q)
2328.15582328.0778341Met-ox 7147331(R)QTNPSAMEVEEDDPVPEIRR(D)
2498.36172498.2779344664871(R) (E)
2518.46652518.4258166166380(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (444/806AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 43/116 matches (37%).
Acc. #: Species: MOUSE Name: TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE (TER ATPASE) (15S MG(2+)-ATPASE P97 SUBUNIT) (VALOSIN CONTAINING PROTEIN) (VCP) [CONTAINS: VALOSIN]
Index: MW: 89309 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
947.6030947.5678371481551(R) (G)
962.5244962.4795477017080(R) (R)
986.5523986.5345182182250(K) (H)
1002.54291002.5294131Met-ox 2182250(K)EMVELPLR(H)
1034.49521034.4366577347410(R) (F)
1049.57671049.5519246696770(K) (M)
1051.56821051.52464146530(K) (G)
1067.54831067.5196271Met-ox 46530(K)MDELQLFR(G)
1075.56341075.5384232782870(K) (K)
1094.66841094.6574103783860(R) (N)
1118.61171118.5806287017091(R) (E)
1172.69271172.6679212402510(R) (T)
1203.64811203.6333122782881(K) (A)
1242.68491242.6330423663770(R) (L)
1265.67961265.6353355135240(K) (T)
1329.74171329.6915384544650(R) (E)
1430.84891430.8293143243360(R) (Q)
1446.86051446.8242251Met-ox 3243360(R)IVSQLLTLMDGLK(Q)
1554.87751554.820237961090(R) (Y)
1556.87871556.8576146396510(R) (S)
1592.86611592.8107355305430(K) (G)
1602.95051602.9253161Met-ox 3233361(R)RIVSQLLTLMDGLK(Q)
1629.85221629.8059287547661(R) (G)
1645.85321645.8008321Met-ox 7547661(R)KYEMFAQTLQQSR(G)
1660.89411660.8295393633771(R) (L)
1695.94751695.8297691Met-ox 6006140(R)VINQILTEMDGMSTK(K)
1695.94751695.9256131Met-ox 2182311(K)EMVELPLRHPALFK(A)
1799.93801799.9907-296396531(R) (V)
1811.02061810.9955142963120(K) (R)
1823.98241823.9180354885020(R) (F)
1823.98241823.9247321Met-ox 6006151(R)VINQILTEMDGMSTKK(N)
1935.99811935.9275365445600(K) (E)
1938.00711937.9683205685840(R) (A)
1951.97741951.9224281Met-ox 5445600(K)GPELLTMWFGESEANVR(E)
2138.06232138.01092466831(R) (M)
2146.19752146.1555203393580(R) (R)
2155.05322154.950148pyroGlu 1Met-ox 7147320(R)QTNPSAMEVEEDDPVPEIR(R)
2162.21862162.1504321Met-ox 3393580(R)AHVIVMAATNRPNSIDPALR(R)
2170.17092170.12432126450(R) (M)
2256.16302256.1375112572770(R) (L)
2272.14692272.13246.41Met-ox 2572770(R)AVANETGAFFFLINGPEIMSK(L)
2318.28892318.2515161Met-ox 3393591(R)AHVIVMAATNRPNSIDPALRR(F)
2318.28892318.27207.33663861(R) (N)
2328.15582328.0778341Met-ox 7147331(R)QTNPSAMEVEEDDPVPEIRR(D)
2498.36172498.2779344664871(R) (E)
2518.46652518.4258166166380(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 52% (424/806AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/116 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 189
Index: MW: 72979 Da pI: 8.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1036.55911036.5441141611680(K) (V)
1110.60941110.6159-5.82122210(K) (H)
1251.68111251.6520235205290(K) (H)
1339.73591339.6983285305410(R) (C)
1513.83261513.7988225485601(K) (I)
1660.89411660.8342363803921(K) (I)
1790.93671790.8933244774910(R) (S)
1816.86411816.9090-253373510(K) (S)
1823.98241823.8533713934071(R) (A)
2294.11452294.1426-123373551(K) (S)
2513.26872513.2836-5.94965151(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (135/626AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/116 matches (10%).
Acc. #: Species: MOUSE Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (Phosphoenolpyruvate carboxylase) (PEPCK-C)
Index: MW: 69355 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1118.61171118.5900195115180(K) (K)
1312.69561312.6295501251351(K) (G)
1554.87751554.7739671140(-) (V)
1570.87581570.7688681Met-ox 1140(-)MPPQLHNGLDFSAK(V)
1830.90271830.9853-455445601(R) (E)
1837.96981838.0387-38921071(K) (T)
1908.97071908.9967-141381550(R) (I)
2122.11772122.1193-0.741361551(K) (I)
2138.06232138.1142-241Met-ox 1361551(K)GRTMYVIPFSMGPLGSPLAK(I)
2196.02362196.1408-531Met-ox 1721911(R)IMTRMGISVLEALGDGEFIK(C)
2284.24052284.1681322Met-ox 1561751(K)IGIELTDSPYVVASMRIMTR(M)
2328.15582328.13887.35255431(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (141/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 15/116 matches (12%).
Acc. #: Species: HUMAN Name: Hypothetical zinc finger protein KIAA1349
Index: MW: 85593 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1049.57671049.54922670781(K) (F)
1051.56821051.5974-284945021(R) (E)
1067.54831067.5923-411Met-ox 4945021(R)AFTKMVNLK(E)
1104.63291104.62655.81331421(R) (S)
1118.61171118.5747336386460(R) (T)
1203.64811203.6486-0.453013101(R) (T)
1446.86051446.7527742142260(K) (I)
1480.83621480.72577552630(R) (I)
1515.81501515.8396-163543661(K) (T)
1561.75361561.8273-471Met-ox 4384501(K)AFMRSSSLIIHQR(I)
1645.85321645.7981332422541(K) (I)
1889.93461889.9366-1.12272411(R) (S)
1908.97071908.8584591711851(R) (T)
1908.97071908.8584595355491(R) (A)
2138.06232138.1134-2479970(R) (C)
2498.36172498.30432376971(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (175/739AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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