MS-Fit Search Results



.

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B23
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2588 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/92(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
17.169e+00721 (22)31.022.838.136.3 89309/5.1MOUSETRANSITIONAL ENDOPLASMIC RETICULUM ATPASE (TER ATPASE) (15S MG(2+)-ATPASE P97 SUBUNIT) (VALOSIN CONTAINING PROTEIN) (VCP) [CONTAINS: VALOSIN]
27.168e+00721 (22)31.022.838.136.3 89323/5.1HUMANTransitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)-ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]
38.187e+00618 (19)34.019.628.337.4 53675/5.5HUMANKeratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
49.156e+00512 (13)21.013.037.564.8 76345/4.6HUMANNucleolin (Protein C23)
51.528e+00511 (11)18.012.06.4175.3 80625/7.3HUMANProtein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
61.010e+00510 (10)18.010.911.565.8 84727/7.3MOUSEComplement C2 precursor (C3/C5 convertase)
79.751e+00412 (13)19.013.0-9.3768.4 81041/10.3HUMANEosinophil peroxidase precursor (EPO)
85.557e+0049 (9)14.09.8-0.50491.4 99104/5.5HUMANCone cGMP-specific 3',5'-cyclic phosphodiesterase alpha'-subunit
94.706e+00411 (11)12.012.022.688.8 97114/6.2MOUSEProminin precursor (Antigen AC133 homolog)
103.844e+0047 (7)34.07.6-5.4360.0 29474/9.2MOUSERibonuclease P protein subunit p30 (RNaseP protein p30) (RNase P subunit 2)
90597.x.......x..x.....x......x....x...xx........x..........x.....xx...x.x.x..x...xx...x.x.....x.
111038.x.......x..x.....x......x....x...xx........x..........x.....xx...x.x.x..x...xx...x.x.....x.
28336x...xx..x..x..x.....xx....x.........x........xxxx..xx...........x......x....................
2267x.....................x.x..x.............x.....................x...x...x....xx.......x..x...
7360........x...................x.....x......xxx..xx.............x.............x....x...........
50675...x.....x.x..x..x.....................................x......x.......x...............x..x..
76649.x..x....x...........x........x.x..............x.........x..x.........x...........x..x......
13125......x............x.............x...............x............x.............x...x.x..x......
6457...........x.......x........x..........x.x........x.x....x......x..........x.x..............
84121......x................x............................x.......x..............x............x.x.



Detailed Results

1. 21/92 matches (22%).
Acc. #: Species: MOUSE Name: TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE (TER ATPASE) (15S MG(2+)-ATPASE P97 SUBUNIT) (VALOSIN CONTAINING PROTEIN) (VCP) [CONTAINS: VALOSIN]
Index: MW: 89309 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1002.56111002.5294321Met-ox 2182250(K)EMVELPLR(H)
1049.58681049.5519336696770(K) (M)
1067.57261067.5196501Met-ox 46530(K)MDELQLFR(G)
1118.61521118.5806317017091(R) (E)
1172.71191172.6679372402510(R) (T)
1190.61451190.5377657337411(R) (F)
1242.67691242.6330353663770(R) (L)
1265.70361265.6353545135240(K) (T)
1329.74871329.6915434544650(R) (E)
1446.85731446.8242231Met-ox 3243360(R)IVSQLLTLMDGLK(Q)
1554.89961554.820251961090(R) (Y)
1556.89581556.8576256396510(R) (S)
1592.87371592.8107405305430(K) (G)
1629.86111629.8059347547661(R) (G)
1645.86231645.8008371Met-ox 7547661(R)KYEMFAQTLQQSR(G)
1695.95381695.8297731Met-ox 6006140(R)VINQILTEMDGMSTK(K)
1695.95381695.9256171Met-ox 2182311(K)EMVELPLRHPALFK(A)
1809.95431809.9897-20941091(R) (Y)
1823.98911823.9180394885020(R) (F)
1823.98911823.9247351Met-ox 6006151(R)VINQILTEMDGMSTKK(N)
1951.99841951.9224391Met-ox 5445600(K)GPELLTMWFGESEANVR(E)
2172.08982171.9767521Met-ox 7147320(R)QTNPSAMEVEEDDPVPEIR(R)
2498.40802498.2779524664871(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (255/806AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 21/92 matches (22%).
Acc. #: Species: HUMAN Name: Transitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)-ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]
Index: MW: 89323 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1002.56111002.5294321Met-ox 2182250(K)EMVELPLR(H)
1049.58681049.5519336696770(K) (M)
1067.57261067.5196501Met-ox 46530(K)MDELQLFR(G)
1118.61521118.5806317017091(R) (E)
1172.71191172.6679372402510(R) (T)
1190.61451190.5377657337411(R) (F)
1242.67691242.6330353663770(R) (L)
1265.70361265.6353545135240(K) (T)
1329.74871329.6915434544650(R) (E)
1446.85731446.8242231Met-ox 3243360(R)IVSQLLTLMDGLK(Q)
1554.89961554.820251961090(R) (Y)
1556.89581556.8576256396510(R) (S)
1592.87371592.8107405305430(K) (G)
1629.86111629.8059347547661(R) (G)
1645.86231645.8008371Met-ox 7547661(R)KYEMFAQTLQQSR(G)
1695.95381695.8297731Met-ox 6006140(R)VINQILTEMDGMSTK(K)
1695.95381695.9256171Met-ox 2182311(K)EMVELPLRHPALFK(A)
1809.95431809.9897-20941091(R) (Y)
1823.98911823.9180394885020(R) (F)
1823.98911823.9247351Met-ox 6006151(R)VINQILTEMDGMSTKK(N)
1951.99841951.9224391Met-ox 5445600(K)GPELLTMWFGESEANVR(E)
2172.08982171.9767521Met-ox 7147320(R)QTNPSAMEVEEDDPVPEIR(R)
2498.40802498.2779524664871(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (255/806AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 18/92 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1000.58881000.5679213173250(R) (G)
1028.56251028.526435pyroGlu 1501580(R)QLETLGQEK(L)
1030.57481030.56866.11231300(K) (T)
1045.58381045.5530291501580(R) (L)
1062.55701062.510844pyroGlu 2262330(R)QLYEEEIR(E)
1079.57771079.5122612652730(K) (S)
1079.57771079.5373372262330(R) (E)
1129.65601129.6217303423521(R) (L)
1129.65601129.6217303533620(K) (A)
1137.61551137.5792322862950(K) (H)
1173.67651173.6367344734830(K) (-)
1277.75281277.7105333823920(K) (K)
1336.69631336.6670221Met-ox 2532640(R)SLDMDSIIAEVK(A)
1341.75561341.74904.93173281(R) (A)
1344.74041344.6759483293410(R) (G)
1368.72441368.6721381Met-ox 1871970(R)TEMENEFVLIK(K)
1405.85201405.8055333823931(K) (L)
1419.81361419.7484462142250(R) (Q)
1566.79941566.79502.81Met-ox 3703811(R)QLREYQELMNVK(L)
1648.83651648.8733-221Met-ox 1181301(K)MLETKWSLLQQQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (165/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/92 matches (13%).
Acc. #: Species: HUMAN Name: Nucleolin (Protein C23)
Index: MW: 76345 Da pI: 4.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1000.58881000.5427463343420(K) (I)
1152.70161152.6853145525611(R) (G)
1160.63281160.5839424584670(R) (G)
1160.63281160.7043-623944031(R) (V)
1178.61631178.5693404114200(K) (L)
1307.73341307.6569586897021(R) (K)
1561.75991561.6811506116240(K) (E)
1594.84671594.7429655245370(K) (E)
1648.83651648.7383603493620(K) (A)
1776.91961776.8332493483621(R) (A)
1809.95431809.8699475225371(K) (E)
2200.09982200.0257345785971(K) (A)
2312.26062312.1563452983180(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (149/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/92 matches (11%).
Acc. #: Species: HUMAN Name: Protein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
Index: MW: 80625 Da pI: 7.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1045.58381045.5464363823910(R) (E)
1179.63451179.60102885940(K) (G)
1242.67691242.7574-652662761(R) (G)
1307.73341307.7099186146240(K) (V)
1307.73341307.73230.785425530(R) (K)
1312.76941312.7517135765861(R) (E)
1319.73011319.6378702332440(R) (G)
1341.75561341.74904.99211(K) (V)
1344.74041344.7752-261251351(R) (K)
1554.89961554.8379404975110(R) (F)
1735.94341735.93832.96947080(R) (G)
1889.99641890.0840-466096241(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (136/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 10/92 matches (10%).
Acc. #: Species: MOUSE Name: Complement C2 precursor (C3/C5 convertase)
Index: MW: 84727 Da pI: 7.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1022.55241022.4981533643720(R) (E)
1049.58681049.5380466876970(K) (R)
1049.58681049.5665193113190(K) (S)
1062.55701062.5618-4.52822901(K) (I)
1079.57771079.5373371681770(R) (V)
1110.59991110.6523-473763850(R) (S)
1446.85731446.8109324854961(R) (E)
1556.89581556.8702167307421(R) (L)
1645.86231645.8437114234361(K) (K)
2211.21772211.20655.02913100(R) (T)
2384.08062384.1823-435826020(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (138/760AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 12/92 matches (13%).
Acc. #: Species: HUMAN Name: Eosinophil peroxidase precursor (EPO)
Index: MW: 81041 Da pI: 10.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1002.56111002.6060-456656731(K) (I)
1028.56251028.6257-611922000(R) (A)
1049.58681049.6108-232682761(K) (N)
1137.61551137.6421-234624710(R) (F)
1190.61451190.6178-2.83843930(K) (E)
1197.73161197.70682178881(K) (A)
1344.74041344.7527-9.25095200(R) (G)
1463.82901463.7528526746860(R) (D)
1522.84291522.8930-331561691(K) (W)
1645.86231645.9138-313273401(R) (F)
1951.99841951.9159422802951(R) (N)
2200.09982200.09731.14534711(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (136/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/92 matches (9%).
Acc. #: Species: HUMAN Name: Cone cGMP-specific 3',5'-cyclic phosphodiesterase alpha'-subunit
Index: MW: 99104 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1036.55691036.5904-3287950(R) (C)
1124.62141124.7043-741781880(K) (E)
1233.66011233.6149371Met-ox 1110(-)MGEINQVAVEK(Y)
1383.75661383.6909483974080(K) (K)
1556.89581556.7929667547660(R) (N)
1776.91961776.8921152502631(K) (A)
1889.99641889.9973-0.446406550(K) (R)
1951.99841952.0646-341331500(K) (K)
2200.09982200.1654-302082251(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (122/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 11/92 matches (11%).
Acc. #: Species: MOUSE Name: Prominin precursor (Antigen AC133 homolog)
Index: MW: 97114 Da pI: 6.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1062.55701062.5981-392592681(K) (D)
1124.62141124.6931-64931020(K) (I)
1179.63451179.5720535155230(K) (K)
1292.73421292.6738472012110(R) (Q)
1307.73341307.6669515155241(K) (L)
1402.76421402.696748pyroGlu 6997100(R)QSVWTLQQTSNK(L)
1419.81361419.7232646997100(R) (L)
1463.82901463.7494542272410(K) (I)
1566.79941566.7797131Met-ox 2662791(K)QTKDALQNMSSSLK(S)
1735.94341735.9854-241Met-ox 1160(-)MALVFSALLLLGLCGK(I)
1809.95431809.8706468518660(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (111/867AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/92 matches (7%).
Acc. #: Species: MOUSE Name: Ribonuclease P protein subunit p30 (RNaseP protein p30) (RNase P subunit 2)
Index: MW: 29474 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1036.55691036.6043-462372460(K) (K)
1157.64961157.64315.62272361(R) (T)
1419.81361419.8364-16971081(R) (T)
1522.84291522.8521-6.1991111(R) (L)
1735.94341735.9529-5.577910(R) (A)
2312.26062312.3117-2247670(R) (S)
2498.40802498.2787521121320(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (92/268AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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