MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.2.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B27-4
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113997
Molecular weight search (1000 - 150000 Da) selects 111891 entries.
Full pI range: 113997 entries.
Species search ( HUMAN MOUSE ) selects 13846 entries.
Combined molecular weight, pI and species searches select 13265 entries.
Pre searches select 13265 entries.

Data Set 1 Results

MS-Fit search selects 1642 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
19.292e+01022 (36)32.036.784.057.1 92470/4.8HUMANEndoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)
26.408e+00820 (33)26.033.381.771.1 92477/4.7MOUSEEndoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa glucose-regulated protein) (GRP94) (ERP99) (Polymorphic tumor rejection antigen 1) (Tumor rejection antigen gp96)
32.233e+00410 (16)13.016.765.573.7 123075/8.1MOUSEDNA MISMATCH REPAIR PROTEIN MSH3 (REPAIR-3 PROTEIN) (REP-1)
41.824e+0049 (15)14.015.065.168.4 82655/6.4HUMANVesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
51.037e+0046 (10)14.010.087.119.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
6709011 (18)16.018.346.1109 104549/6.2MOUSEExostosin-like 3 (Multiple exostosis-like protein 3)
757068 (13)11.013.364.888.3 83935/6.4HUMANDystrobrevin alpha (Dystrobrevin-alpha)
8511511 (18)10.018.369.262.2 146121/6.2HUMAN1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 2 (PLC-gamma-2) (Phospholipase C-gamma-2) (PLC-IV)
945508 (13)7.013.357.961.2 133611/6.3HUMANIntegrin alpha-11 precursor
10431911 (18)10.018.344.793.7 138073/8.1HUMANEndothelial cell multimerin precursor
65622.xxxxxx..x.x...xxx..x..x.........x.xxx......x....x.x....x..x
108789.xxxxxx..x.x...xxx..x..x.........x.x.x......xx...x.........x
84151...xx..x.x....................x....x.......x...x.......x...x
16307.....xx..x.....................x.......x.x....x.x.x.........
45632...................x........x...x.......x.........x.......x.
107182....x.x......x...x..xx.x........x...x.............x.x.......
3086.....................xx....x.....xx.....x.x..x..............
47712..xx............x.......x.xx...x.x..x..xx...................
23456....x.x.........................x....x..x.....x.x.x.........
59539.....x.....xx..............x..x....x.x.......x..x......x..x.


Detailed Results

1. 22/60 matches (36%).
Acc. #: Species: HUMAN Name: Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)
Index: MW: 92470 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
876.5347876.4579886846900(K) (H)
919.6118919.536582961021(K) (E)
982.5839982.48461011691770(K) (M)
1015.57031015.4737953964040(R) (K)
1029.60761029.60571.86316391(K) (L)
1031.60191031.49841005385460(K) (K)
1047.60391047.49331061Met-ox 5385460(K)IYFMAGSSR(K)
1081.64361081.54319376840(K) (M)
1139.66481139.5809744945030(K) (T)
1143.68841143.56861053964051(R) (S)
1150.65381150.53801015485570(K) (L)
1150.65381150.65191.66916991(R) (R)
1187.78341187.6788883853950(K) (G)
1278.78151278.63301165475571(R) (L)
1485.88831485.7549904354480(K) (E)
1485.88831485.8099537427541(R) (V)
1513.90191513.7797811431560(K) (E)
1515.85511515.7120944754861(K) (E)
1529.89431529.7746781Met-ox 1431560(K)NLLHVTDTGVGMTR(E)
1784.02771783.81481192Met-ox 4154281(R)RVFITDDFHDMMPK(Y)
1879.11971878.93121001Met-ox 6096231(R)EAVEKEFEPLLNWMK(D)
1879.11971878.9430942712850(K) (T)
2046.21382046.0607751171350(R) (I)
2260.25762260.0634865125300(R) (M)
2728.55592728.33908044671(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (260/803AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 20/60 matches (33%).
Acc. #: Species: MOUSE Name: Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa glucose-regulated protein) (GRP94) (ERP99) (Polymorphic tumor rejection antigen 1) (Tumor rejection antigen gp96)
Index: MW: 92477 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
876.5347876.4579886846900(K) (H)
919.6118919.536582961021(K) (E)
982.5839982.48461011691770(K) (M)
1015.57031015.4737953964040(R) (K)
1029.60761029.60571.86316391(K) (L)
1031.60191031.49841005385460(K) (K)
1047.60391047.49331061Met-ox 5385460(K)IYFMAGSSR(K)
1081.64361081.54319376840(K) (M)
1139.66481139.5809744945030(K) (T)
1143.68841143.56861053964051(R) (S)
1150.65381150.53801015485570(K) (L)
1150.65381150.65191.66916991(R) (R)
1187.78341187.6788883853950(K) (G)
1278.78151278.63301165475571(R) (L)
1485.88831485.7549904354480(K) (E)
1513.90191513.7797811431560(K) (E)
1529.89431529.72761094754861(K) (E)
1529.89431529.7746781Met-ox 1431560(K)NLLHVTDTGVGMTR(E)
1784.02771783.81481192Met-ox 4154281(R)RVFITDDFHDMMPK(Y)
1794.95871794.9675-4.91Met-ox 7097240(K)TVMDLAVVLFETATLR(S)
1879.11971878.9430942712850(K) (T)
2728.55592728.33908044671(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (210/802AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/60 matches (16%).
Acc. #: Species: MOUSE Name: DNA MISMATCH REPAIR PROTEIN MSH3 (REPAIR-3 PROTEIN) (REP-1)
Index: MW: 123075 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
982.5839982.46681198939010(R) (G)
1015.57031015.5788-8.3104610540(K) (R)
1036.62941036.5679596516591(K) (D)
1047.60391047.5726306606680(K) (K)
1411.80931411.65281111321420(R) (A)
1513.90191513.8089615906011(K) (S)
1765.93091765.8365531031161(K) (V)
1805.05621804.88309674891(K) (C)
1805.05621804.9499597317451(K) (R)
2233.27942233.0819884764941(K) (I)
2728.55592728.4157515525751(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (147/1091AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Vesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
Index: MW: 82655 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1029.60761029.551554pyroGlu 1Met-ox 2722801(R)QIGKMLNAR(E)
1031.60191031.5083913934010(K) (G)
1047.60391047.5032961Met-ox 3934010(K)MEIGLPDEK(G)
1414.92071414.8395574024131(K) (M)
1571.02011570.9936176186310(R) (K)
1684.96001684.8369732182321(K) (R)
1800.03931800.0206102552711(K) (Q)
1811.04771810.908377901040(K) (K)
1994.14071993.921111011270(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (108/744AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.70111179.6010853773860(K) (H)
1383.81511383.6909901861970(K) (V)
1475.89921475.74941012122230(K) (T)
1475.89921475.7858772002110(R) (W)
1638.98591638.8604771861991(K) (F)
1994.14071993.9772822242390(R) (R)
2384.18562383.9524985195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (95/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 11/60 matches (18%).
Acc. #: Species: MOUSE Name: Exostosin-like 3 (Multiple exostosis-like protein 3)
Index: MW: 104549 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1015.57031015.5788-8.34184251(R) (L)
1031.60191031.5373631011090(R) (L)
1031.60191031.5890136826900(R) (V)
1091.66621091.5883714404490(R) (L)
1150.65381150.5857595465550(R) (S)
1187.78341187.63841221001091(K) (L)
1235.70841235.5942921Met-ox 6726810(R)EEVLMNSLER(L)
1235.70841235.7588-415355451(R) (E)
1278.78151278.8149-261531631(K) (D)
1475.89921475.7719862682800(R) (K)
1515.85511515.7517686606710(R) (E)
1994.14071993.90851161Met-ox 9261(R)NGGVGNGGQTCMLRWSNR(I)
1994.14071994.0269573844001(K) (N)
2097.17452097.2324-284214391(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (153/918AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Dystrobrevin alpha (Dystrobrevin-alpha)
Index: MW: 83935 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1235.70841235.6384573914010(K) (L)
1251.76261251.6810654304391(R) (Y)
1350.79951350.65411087327430(K) (-)
1485.88831485.72981074444570(R) (S)
1493.83911493.7236774955070(R) (A)
1638.98591638.91074681940(R) (R)
1707.94881707.7972891Met-ox 5265391(K)DELEQRMSALQESR(R)
1794.95871795.0118-3081951(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (89/743AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 11/60 matches (18%).
Acc. #: Species: HUMAN Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 2 (PLC-gamma-2) (Phospholipase C-gamma-2) (PLC-IV)
Index: MW: 146121 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
919.6118919.5365826666731(R) (R)
982.5839982.5369485835891(R) (S)
1143.68841143.6162631851941(K) (D)
1307.78301307.69217061710(K) (E)
1348.78951348.64971049439531(K) (E)
1348.78951348.7476318518620(R) (A)
1350.79951350.7105666096181(R) (E)
1414.92071414.882027105910711(K) (H)
1485.88831485.8429317317421(R) (Y)
1515.85511515.7266856196300(R) (L)
1571.02011570.830312121340(R) (K)
1638.98591638.8161104103410471(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (134/1252AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Integrin alpha-11 precursor
Index: MW: 133611 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1015.57031015.5723-1.9110411120(K) (Q)
1031.60191031.5672341Met-ox 110411120(K)IMVNAALQR(Q)
1475.89921475.7528997197310(R) (I)
1529.89431529.8110541011141(K) (D)
1638.98591638.8644749499611(R) (S)
1800.03931799.9267638889011(R) (A)
1811.04771810.9088774204360(K) (Q)
1994.14071994.0129647157311(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (84/1189AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/60 matches (18%).
Acc. #: Species: HUMAN Name: Endothelial cell multimerin precursor
Index: MW: 138073 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1029.60761029.6421-343803881(K) (D)
1081.64361081.6046367968040(R) (T)
1084.64691084.63669.49899990(R) (S)
1350.79951350.6654998128221(R) (M)
1411.80931411.8637-38101610280(K) (T)
1513.90191513.7949712592701(R) (C)
1529.89431529.7899681Met-ox 2592701(R)MQHKIVTSLDWR(C)
1794.95871794.8155802Met-ox 8238361(K)MYQMFNETTSQVRK(Y)
1794.95871794.8696507167301(R) (T)
1811.04771810.86451011Met-ox 7167301(R)RINEYALEMEDGLNK(T)
2233.27942233.1213714804981(K) (S)
2384.18562384.1300233Met-ox 5555731(K)KQSLMMLQMFEDLHIQESK(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (132/1228AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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