MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.2.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B28
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113997
Molecular weight search (1000 - 100000 Da) selects 107696 entries.
Full pI range: 113997 entries.
Species search ( HUMAN MOUSE ) selects 13846 entries.
Combined molecular weight, pI and species searches select 12267 entries.
Pre searches select 12267 entries.

Data Set 1 Results

MS-Fit search selects 4238 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/120(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.359e+01531 (25)42.025.897.355.9 92470/4.8HUMANEndoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)
23.052e+01430 (25)36.025.098.158.8 92477/4.7MOUSEEndoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa glucose-regulated protein) (GRP94) (ERP99) (Polymorphic tumor rejection antigen 1) (Tumor rejection antigen gp96)
31.445e+00717 (14)21.014.286.651.7 95339/6.4HUMANElongation factor 2 (EF-2)
46.925e+00616 (13)33.013.372.061.9 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
55.862e+00616 (13)22.013.382.265.6 95315/6.4MOUSEElongation factor 2 (EF-2)
65622x.x....xx...x.xx..x..x....xxx....x..........x............x..xxxx.....xx....x.xxx.........x...............x...x...x....xx
108789x.x....xx...x.xx..x..x....xxx....x..........x............x..x.xx.....xx....x.xxx.x.......x...................x...x....xx
56186...x.x.......x.....x.....x.............x...x......x.x..x................xx........xx..........x..x..x...................
85041...............x.......xx..xx.x.............x......x.......x.x..................x......xx...x.....x...............x.....
38197...x...............x...x.x.............x...x......x.x..x................xx........xx..........x..x..x...................


Detailed Results

1. 31/120 matches (25%).
Acc. #: Species: HUMAN Name: Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)
Index: MW: 92470 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.5886864.48651181Met-ox 4674731(R)KTLDMIK(K)
864.5886864.48651181Met-ox 4684741(K)TLDMIKK(I)
876.5483876.45791036846900(K) (H)
919.6333919.5365105961021(K) (E)
963.6210963.58793488950(K) (N)
1004.63661004.5529836836901(K) (H)
1015.58501015.47371103964040(R) (K)
1031.61771031.49841165385460(K) (K)
1047.63461047.49331351Met-ox 5385460(K)IYFMAGSSR(K)
1081.65961081.543110876840(K) (M)
1139.67941139.5809864945030(K) (T)
1143.69911143.56861143964051(R) (S)
1150.65981150.53801065485570(K) (L)
1150.65981150.65196.86916991(R) (R)
1187.80281187.67881043853950(K) (G)
1278.77331278.63301105475571(R) (L)
1485.91471485.75491084354480(K) (E)
1485.91471485.8099717427541(R) (V)
1513.91351513.7797881431560(K) (E)
1515.88871515.71201174754861(K) (E)
1529.91491529.7746921Met-ox 1431560(K)NLLHVTDTGVGMTR(E)
1544.97681544.8284961031161(R) (L)
1595.89211595.72381054164280(R) (Y)
1627.90771627.71361192Met-ox 4164280(R)VFITDDFHDMMPK(Y)
1768.02051767.81981141Met-ox 4154281(R)RVFITDDFHDMMPK(Y)
1784.05711783.81481362Met-ox 4154281(R)RVFITDDFHDMMPK(Y)
1786.06071785.89839152670(R) (E)
1787.05181786.9122781Met-ox 1411561(K)EKNLLHVTDTGVGMTR(E)
1879.08801878.9312831Met-ox 6096231(R)EAVEKEFEPLLNWMK(D)
1879.08801878.9430772712850(K) (T)
2260.30332260.06341065125300(R) (M)
2287.35512287.02291453653840(K) (S)
2287.35512287.2397501171371(R) (C)
2370.34312370.08581096406600(R) (I)
2711.35202711.2086532152370(K) (G)
2728.63092728.339010744671(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (341/803AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 30/120 matches (25%).
Acc. #: Species: MOUSE Name: Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa glucose-regulated protein) (GRP94) (ERP99) (Polymorphic tumor rejection antigen 1) (Tumor rejection antigen gp96)
Index: MW: 92477 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.5886864.48651181Met-ox 4674731(R)KTLDMIK(K)
864.5886864.48651181Met-ox 4684741(K)TLDMIKK(I)
876.5483876.45791036846900(K) (H)
919.6333919.5365105961021(K) (E)
963.6210963.58793488950(K) (N)
1004.63661004.5529836836901(K) (H)
1015.58501015.47371103964040(R) (K)
1031.61771031.49841165385460(K) (K)
1047.63461047.49331351Met-ox 5385460(K)IYFMAGSSR(K)
1081.65961081.543110876840(K) (M)
1139.67941139.5809864945030(K) (T)
1143.69911143.56861143964051(R) (S)
1150.65981150.53801065485570(K) (L)
1150.65981150.65196.86916991(R) (R)
1187.80281187.67881043853950(K) (G)
1278.77331278.63301105475571(R) (L)
1485.91471485.75491084354480(K) (E)
1513.91351513.7797881431560(K) (E)
1529.91491529.72761224754861(K) (E)
1529.91491529.7746921Met-ox 1431560(K)NLLHVTDTGVGMTR(E)
1544.97681544.8284961031161(R) (L)
1595.89211595.72381054164280(R) (Y)
1627.90771627.71361192Met-ox 4164280(R)VFITDDFHDMMPK(Y)
1768.02051767.81981141Met-ox 4154281(R)RVFITDDFHDMMPK(Y)
1784.05711783.81481362Met-ox 4154281(R)RVFITDDFHDMMPK(Y)
1786.06071785.89839152670(R) (E)
1787.05181786.9122781Met-ox 1411561(K)EKNLLHVTDTGVGMTR(E)
1795.04001794.9675401Met-ox 7097240(K)TVMDLAVVLFETATLR(S)
1879.08801878.9430772712850(K) (T)
2287.35512287.02291453653840(K) (S)
2370.34312370.08581096406600(R) (I)
2711.35202711.2086532152370(K) (G)
2728.63092728.339010744671(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (289/802AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 17/120 matches (14%).
Acc. #: Species: HUMAN Name: Elongation factor 2 (EF-2)
Index: MW: 95339 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
890.5980890.5100994995060(K) (V)
904.5914904.47401302762841(K) (L)
1013.61101013.50161082402490(K) (A)
1050.59661050.5584365725801(K) (E)
1137.69251137.58259733420(K) (A)
1222.72241222.6254791100(-) (A)
1274.85211274.71091114404490(K) (T)
1348.79401348.7047661Met-ox 4274381(K)VRIMGPNYTPGK(K)
1392.88251392.73091097287390(R) (L)
1475.89571475.7901721511621(R) (A)
1708.00091707.874074AcetN 1Met-ox 1151(-)VNFTVDQIRAIMDK(K)
1742.03381741.9821301451591(R) (M)
1800.07151799.8968977868010(K) (S)
1813.07631812.8988982Met-ox 1151(-)MVNFTVDQIRAIMDK(K)
1962.14451961.9795847687850(R) (A)
2143.29302143.07841006066251(K) (Q)
2220.33862220.1552831631800(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (185/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 16/120 matches (13%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1031.61771031.5638523153221(R) (R)
1128.69091128.6741155105200(K) (V)
1132.66641132.5850721561650(R) (T)
1143.69911143.56861141721810(K) (L)
1150.65981150.5897614414500(K) (A)
1174.74701174.58571374674760(K) (N)
1278.77331278.6846694404501(K) (A)
1350.82501350.7568513083181(K) (W)
1510.95301510.8593624074211(K) (Q)
1515.88871515.8131501531651(K) (T)
1791.09361790.89991081661811(R) (L)
1839.10891838.9574821211370(R) (K)
1852.08631851.90239938541(K) (M)
1911.16511911.0050841Met-ox 2072240(K)ILGSGIYSSSVLHGMVFK(K)
2200.18982200.0960434224401(K) (F)
2384.23832384.1114532612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (182/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 16/120 matches (13%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
890.5980890.5100994995060(K) (V)
1050.59661050.5584365725801(K) (E)
1128.69091128.55121241Met-ox 3003080(K)VFDAIMNFR(K)
1137.69251137.55401222732831(K) (K)
1137.69251137.58259733420(K) (A)
1222.72241222.6254791100(-) (A)
1274.85211274.71091114404490(K) (T)
1348.79401348.7047661Met-ox 4274381(K)VRIMGPNYTPGK(K)
1348.79401348.7953-0.904164281(R) (I)
1392.88251392.73091097287390(R) (L)
1475.89571475.7901721511621(R) (A)
1708.00091707.874074AcetN 1Met-ox 1151(-)VNFTVDQIRAIMDK(K)
1742.03381741.9821301451591(R) (M)
1800.07151799.8968977868010(K) (S)
1813.07631812.8988982Met-ox 1151(-)MVNFTVDQIRAIMDK(K)
1962.14451961.9795847687850(R) (A)
2143.29302143.07841006066251(K) (Q)
2220.33862220.1552831631800(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (197/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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