MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B32
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 150000 Da) selects 114669 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 13650 entries.
Pre searches select 13650 entries.

Data Set 1 Results

MS-Fit search selects 4070 entries (results displayed for top 10 matches).

Results Summary

	MOWSE Score	#/94(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	7.912e+013	26 (27)	25.0	27.7	30.9	33.8	117791/5.6	HUMAN	Ubiquitin-activating enzyme E1 (A1S9 protein)
2	5.689e+008	18 (19)	17.0	19.1	29.3	36.7	117810/5.4	MOUSE	Ubiquitin-activating enzyme E1 1
3	1.384e+007	18 (19)	31.0	19.1	25.3	44.9	53675/5.5	HUMAN	Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
4	6.325e+006	13 (13)	25.0	13.8	35.4	44.9	50986/6.3	MOUSE	Ubiquitin-activating enzyme E1 X
5	5.703e+005	14 (14)	17.0	14.9	14.7	71.6	119389/6.2	HUMAN	Huntingtin interacting protein 1 related (Hip1-related) (Hip 12)
6	4.964e+005	14 (14)	19.0	14.9	20.6	58.0	92890/5.3	HUMAN	DNA replication licensing factor MCM6 (P105MCM)
7	1.787e+005	11 (11)	18.0	11.7	12.3	81.3	71370/8.6	HUMAN	Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin) (Fletcher factor)
8	6.843e+004	14 (14)	17.0	14.9	17.1	59.0	74210/6.5	MOUSE	Lamin A
9	6.290e+004	11 (11)	23.0	11.7	16.0	39.8	48058/5.3	HUMAN	Keratin, type I cytoskeletal 18 (Cytokeratin 18) (K18) (CK 18)
10	5.167e+004	11 (11)	9.0	11.7	13.9	88.1	122140/8.1	HUMAN	DNA-directed RNA polymerase I 135 kDa polypeptide (RNA polymerase I subunit 2) (RPA135)

13892	.	.	x	.	.	.	x	x	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	x	.	.	.	.	.	x	.	.	.	x	x	x	.	x	.	.	.	.	x	x	.	x	.	.	x	.	x	x	x	x	x	.	x	.
89732	.	.	x	.	.	.	x	x	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	x	x	.	x	.	.	.	.	x	x	.	.	.	.	x	.	.	x	.	.	.	.	x	.
28336	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	.	.	x	.	.	.	x	x	.	.	.	x	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.	x	.	.	x	.	.	x	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.
87103	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	.	x	.	.	.	.	.	.
10131	.	.	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	x
37857	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.
21973	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.
14532	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
50879	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.
58850	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 26/94 matches (27%).
Acc. #: Species: HUMAN Name: Ubiquitin-activating enzyme E1 (A1S9 protein)
Index: MW: 117791 Da pI: 5.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
930.4789	930.4620	18		587	592	1	(R) (K)
943.4698	943.4565	14	pyroGlu	974	980	0	(K)QFLDYFK(T)
945.5407	945.5443	-3.8		628	635	0	(K) (N)
960.4929	960.4750	19		120	127	1	(R) (A)
960.4929	960.4831	10		974	980	0	(K) (T)
984.5143	984.4678	47		650	657	0	(R) (Q)
1032.5139	1032.4685	44		175	182	0	(R) (G)
1102.6103	1102.6220	-11		527	537	1	(K) (Q)
1250.6251	1250.5653	48		870	880	0	(R) (H)
1259.7803	1259.7323	38		70	81	0	(R) (G)
1288.7160	1288.6571	46		1011	1021	0	(R) (V)
1288.7160	1288.6724	34		58	68	0	(R) (R)
1304.6950	1304.6520	33	1Met-ox	1011	1021	0	(R)LDQPMTEIVSR(V)
1304.6950	1304.6673	21	1Met-ox	58	68	0	(R)QLYVLGHEAMK(R)
1370.7894	1370.7684	15		831	843	1	(K) (M)
1415.8490	1415.8334	11		69	81	1	(K) (G)
1419.7981	1419.7425	39	pyroGlu	516	526	0	(R)QFLFRPWDVTK(L)
1436.8188	1436.7690	35		516	526	0	(R) (L)
1459.7398	1459.6851	37		227	239	0	(K) (H)
1573.8559	1573.8008	35		1009	1021	1	(K) (V)
1623.9627	1623.9070	34		679	693	0	(R) (S)
1655.8453	1655.7917	32		451	465	0	(R) (L)
1696.9206	1696.8560	38		636	649	0	(K) (D)
1809.0179	1808.9507	37		369	384	0	(R) (K)
1838.9519	1838.8251	69		945	957	0	(R) (F)
1884.0234	1883.9463	41		351	368	0	(R) (A)
1909.0422	1908.9959	24		305	322	0	(K) (F)
1937.1453	1937.0456	51		369	385	1	(R) (L)
2369.2016	2369.1124	38		269	288	0	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (271/1058AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 18/94 matches (19%).
Acc. #: Species: MOUSE Name: Ubiquitin-activating enzyme E1 1
Index: MW: 117810 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
930.4789 930.4620 18 587 592 1 (R) (K)
943.4698 943.4565 14 pyroGlu 974 980 0 (K)QFLDYFK(T)
945.5407 945.5443 -3.8 628 635 0 (K) (N)
960.4929 960.4750 19 120 127 1 (R) (A)
960.4929 960.4831 10 974 980 0 (K) (T)
984.5143 984.4678 47 650 657 0 (R) (Q)
1102.6103 1102.6220 -11 527 537 1 (K) (Q)
1250.6251 1250.5653 48 870 880 0 (R) (H)
1288.7160 1288.6571 46 1011 1021 0 (R) (V)
1288.7160 1288.6724 34 58 68 0 (R) (M)
1304.6950 1304.6520 33 1Met-ox 1011 1021 0 (R)LDQPMTEIVSR(V)
1304.6950 1304.6673 21 1Met-ox 58 68 0 (R)QLYVLGHEAMK(M)
1370.7894 1370.7684 15 831 843 1 (K) (M)
1419.7981 1419.7425 39 pyroGlu 516 526 0 (R)QFLFRPWDVTK(L)
1436.8188 1436.7690 35 516 526 0 (R) (L)
1459.7398 1459.6851 37 227 239 0 (K) (H)
1573.8559 1573.8008 35 1009 1021 1 (K) (V)
1623.9627 1623.9070 34 679 693 0 (R) (S)
1696.9206 1696.8560 38 636 649 0 (K) (D)
1838.9519 1838.8251 69 945 957 0 (R) (F)
2369.2016 2369.1124 38 269 288 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (182/1058AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 18/94 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1000.5311 1000.4998 31 pyroGlu 365 372 1 (K)QDMARQLR(E)
1000.5311 1000.5679 -37 317 325 0 (R) (G)
1016.5149 1016.4947 20 pyroGlu 1Met-ox 365 372 1 (K)QDMARQLR(E)
1030.5702 1030.5686 1.6 123 130 0 (K) (T)
1045.5578 1045.5530 4.7 150 158 0 (R) (L)
1060.5750 1060.5639 11 393 401 1 (R) (L)
1062.5497 1062.5108 37 pyroGlu 226 233 0 (R)QLYEEEIR(E)
1079.5665 1079.5122 50 265 273 0 (K) (S)
1079.5665 1079.5373 27 226 233 0 (R) (E)
1129.6608 1129.6217 35 342 352 1 (R) (L)
1129.6608 1129.6217 35 353 362 0 (K) (A)
1137.6283 1137.5792 43 286 295 0 (K) (H)
1153.6405 1153.5563 73 373 381 0 (R) (L)
1277.7547 1277.7105 35 382 392 0 (K) (K)
1341.7648 1341.7490 12 317 328 1 (R) (A)
1344.7264 1344.6759 37 329 341 0 (R) (G)
1405.8208 1405.8055 11 382 393 1 (K) (L)
1419.7981 1419.7484 35 214 225 0 (R) (Q)
1459.7398 1459.6930 32 19 32 1 (R) (I)
1566.7981 1566.7950 2.0 1Met-ox 370 381 1 (R)QLREYQELMNVK(L)
1685.9185 1685.8571 36 326 341 1 (K) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1000.5311	1000.4998	31	pyroGlu	365	372	1	(K)QDMARQLR(E)
1000.5311	1000.5679	-37		317	325	0	(R) (G)
1016.5149	1016.4947	20	pyroGlu 1Met-ox	365	372	1	(K)QDMARQLR(E)
1030.5702	1030.5686	1.6		123	130	0	(K) (T)
1045.5578	1045.5530	4.7		150	158	0	(R) (L)
1060.5750	1060.5639	11		393	401	1	(R) (L)
1062.5497	1062.5108	37	pyroGlu	226	233	0	(R)QLYEEEIR(E)
1079.5665	1079.5122	50		265	273	0	(K) (S)
1079.5665	1079.5373	27		226	233	0	(R) (E)
1129.6608	1129.6217	35		342	352	1	(R) (L)
1129.6608	1129.6217	35		353	362	0	(K) (A)
1137.6283	1137.5792	43		286	295	0	(K) (H)
1153.6405	1153.5563	73		373	381	0	(R) (L)
1277.7547	1277.7105	35		382	392	0	(K) (K)
1341.7648	1341.7490	12		317	328	1	(R) (A)
1344.7264	1344.6759	37		329	341	0	(R) (G)
1405.8208	1405.8055	11		382	393	1	(K) (L)
1419.7981	1419.7484	35		214	225	0	(R) (Q)
1459.7398	1459.6930	32		19	32	1	(R) (I)
1566.7981	1566.7950	2.0	1Met-ox	370	381	1	(R)QLREYQELMNVK(L)
1685.9185	1685.8571	36		326	341	1	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (153/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/94 matches (13%).
Acc. #: Species: MOUSE Name: Ubiquitin-activating enzyme E1 X
Index: MW: 50986 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
943.4698 943.4565 14 pyroGlu 360 366 0 (K)QFLDYFK(T)
945.5407 945.5443 -3.8 14 21 0 (K) (N)
960.4929 960.4831 10 360 366 0 (K) (T)
984.5143 984.4678 47 36 43 0 (R) (Q)
1250.6251 1250.5653 48 256 266 0 (R) (H)
1288.7160 1288.6571 46 397 407 0 (R) (V)
1304.6950 1304.6520 33 1Met-ox 397 407 0 (R)LDQPMTEIVSR(V)
1370.7894 1370.7684 15 217 229 1 (K) (M)
1408.7215 1408.6176 74 1Met-ox 419 429 0 (R)HWCLSCAATMK(A)
1573.8559 1573.8008 35 395 407 1 (K) (V)
1623.9627 1623.9070 34 65 79 0 (R) (S)
1696.9206 1696.8560 38 22 35 0 (K) (D)
1838.9519 1838.8251 69 331 343 0 (R) (F)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
943.4698	943.4565	14	pyroGlu	360	366	0	(K)QFLDYFK(T)
945.5407	945.5443	-3.8		14	21	0	(K) (N)
960.4929	960.4831	10		360	366	0	(K) (T)
984.5143	984.4678	47		36	43	0	(R) (Q)
1250.6251	1250.5653	48		256	266	0	(R) (H)
1288.7160	1288.6571	46		397	407	0	(R) (V)
1304.6950	1304.6520	33	1Met-ox	397	407	0	(R)LDQPMTEIVSR(V)
1370.7894	1370.7684	15		217	229	1	(K) (M)
1408.7215	1408.6176	74	1Met-ox	419	429	0	(R)HWCLSCAATMK(A)
1573.8559	1573.8008	35		395	407	1	(K) (V)
1623.9627	1623.9070	34		65	79	0	(R) (S)
1696.9206	1696.8560	38		22	35	0	(K) (D)
1838.9519	1838.8251	69		331	343	0	(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (113/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/94 matches (14%).
Acc. #: Species: HUMAN Name: Huntingtin interacting protein 1 related (Hip1-related) (Hip 12)
Index: MW: 119389 Da pI: 6.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
941.4645 941.4991 -37 747 754 0 (R) (T)
945.5407 945.5257 16 353 360 0 (R) (R)
960.4929 960.5366 -45 511 518 1 (K) (R)
994.5477 994.4958 52 pyroGlu 1050 1057 1 (R)QDHQLDKK(D)
1104.6086 1104.5788 27 502 510 1 (R) (S)
1129.6608 1129.6152 40 1 10 1 (-) (V)
1139.5871 1139.6213 -30 282 291 0 (R) (A)
1184.6544 1184.6315 19 126 135 0 (R) (L)
1459.7398 1459.7869 -32 941 954 1 (R) (S)
1471.7754 1471.7195 38 251 261 1 (R) (N)
1471.7754 1471.7756 -0.15 626 641 0 (R) (L)
1713.9479 1713.8958 30 732 746 0 (R) (H)
1909.0422 1908.9707 37 147 163 0 (K) (A)
2369.2016 2369.1295 30 955 975 1 (K) (L)
2683.5268 2683.3296 74 189 212 1 (K) (L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
941.4645	941.4991	-37		747	754	0	(R) (T)
945.5407	945.5257	16		353	360	0	(R) (R)
960.4929	960.5366	-45		511	518	1	(K) (R)
994.5477	994.4958	52	pyroGlu	1050	1057	1	(R)QDHQLDKK(D)
1104.6086	1104.5788	27		502	510	1	(R) (S)
1129.6608	1129.6152	40		1	10	1	(-) (V)
1139.5871	1139.6213	-30		282	291	0	(R) (A)
1184.6544	1184.6315	19		126	135	0	(R) (L)
1459.7398	1459.7869	-32		941	954	1	(R) (S)
1471.7754	1471.7195	38		251	261	1	(R) (N)
1471.7754	1471.7756	-0.15		626	641	0	(R) (L)
1713.9479	1713.8958	30		732	746	0	(R) (H)
1909.0422	1908.9707	37		147	163	0	(K) (A)
2369.2016	2369.1295	30		955	975	1	(K) (L)
2683.5268	2683.3296	74		189	212	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (189/1068AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 14/94 matches (14%).
Acc. #: Species: HUMAN Name: DNA replication licensing factor MCM6 (P105MCM)
Index: MW: 92890 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
930.4789 930.4645 15 pyroGlu 603 611 1 (R)QRDGSGVTK(S)
984.5143 984.4977 17 86 92 0 (R) (A)
995.5517 995.5750 -23 551 558 1 (R) (I)
1000.5311 1000.4852 46 408 415 0 (K) (A)
1000.5311 1000.5111 20 182 189 1 (R) (R)
1000.5311 1000.5904 -59 487 496 1 (K) (T)
1065.5630 1065.5151 45 174 181 0 (K) (N)
1136.6265 1136.6064 18 566 574 1 (R) (Y)
1177.7712 1177.7421 25 723 732 0 (R) (K)
1184.6544 1184.6275 23 208 217 0 (R) (G)
1292.7196 1292.6309 69 327 336 1 (K) (V)
1307.7242 1307.7112 10.0 745 754 1 (K) (E)
1381.7050 1381.6599 33 270 282 0 (R) (G)
1471.7754 1471.7222 36 109 120 0 (K) (H)
1629.9347 1629.8964 23 46 58 0 (K) (N)
1685.9185 1685.8611 34 497 512 0 (R) (S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
930.4789	930.4645	15	pyroGlu	603	611	1	(R)QRDGSGVTK(S)
984.5143	984.4977	17		86	92	0	(R) (A)
995.5517	995.5750	-23		551	558	1	(R) (I)
1000.5311	1000.4852	46		408	415	0	(K) (A)
1000.5311	1000.5111	20		182	189	1	(R) (R)
1000.5311	1000.5904	-59		487	496	1	(K) (T)
1065.5630	1065.5151	45		174	181	0	(K) (N)
1136.6265	1136.6064	18		566	574	1	(R) (Y)
1177.7712	1177.7421	25		723	732	0	(R) (K)
1184.6544	1184.6275	23		208	217	0	(R) (G)
1292.7196	1292.6309	69		327	336	1	(K) (V)
1307.7242	1307.7112	10.0		745	754	1	(K) (E)
1381.7050	1381.6599	33		270	282	0	(R) (G)
1471.7754	1471.7222	36		109	120	0	(K) (H)
1629.9347	1629.8964	23		46	58	0	(K) (N)
1685.9185	1685.8611	34		497	512	0	(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 19% (161/821AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 11/94 matches (11%).
Acc. #: Species: HUMAN Name: Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin) (Fletcher factor)
Index: MW: 71370 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
935.4659 935.4838 -19 460 467 0 (K) (E)
941.4645 941.5031 -41 74 80 1 (K) (D)
963.4735 963.4570 17 351 359 0 (R) (I)
1077.5072 1077.5693 -58 605 613 1 (R) (V)
1079.5665 1079.5043 58 131 139 0 (K) (R)
1157.6260 1157.6319 -5.1 468 476 0 (K) (V)
1381.7050 1381.6217 60 103 113 0 (K) (D)
1419.7981 1419.7055 65 592 604 0 (R) (R)
1436.8188 1436.8001 13 447 459 0 (R) (D)
1459.7398 1459.7181 15 360 373 0 (R) (L)
1713.9479 1713.8958 30 538 551 1 (K) (Y)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
935.4659	935.4838	-19	460	467	0	(K) (E)
941.4645	941.5031	-41	74	80	1	(K) (D)
963.4735	963.4570	17	351	359	0	(R) (I)
1077.5072	1077.5693	-58	605	613	1	(R) (V)
1079.5665	1079.5043	58	131	139	0	(K) (R)
1157.6260	1157.6319	-5.1	468	476	0	(K) (V)
1381.7050	1381.6217	60	103	113	0	(K) (D)
1419.7981	1419.7055	65	592	604	0	(R) (R)
1436.8188	1436.8001	13	447	459	0	(R) (D)
1459.7398	1459.7181	15	360	373	0	(R) (L)
1713.9479	1713.8958	30	538	551	1	(K) (Y)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 18% (116/638AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 14/94 matches (14%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
935.4659 935.4798 -15 420 427 1 (R) (S)
972.5514 972.5114 41 389 397 0 (R) (S)
1032.5139 1032.5148 -0.84 182 189 0 (K) (R)
1048.5436 1048.5097 32 1Met-ox 182 189 0 (K)QLQDEMLR(R)
1089.5915 1089.5540 34 51 60 0 (R) (L)
1102.6103 1102.5744 33 378 386 1 (R) (L)
1104.6086 1104.6530 -40 42 50 1 (R) (S)
1187.6886 1187.6384 42 320 329 1 (K) (E)
1344.7264 1344.7236 2.1 49 60 1 (R) (L)
1350.7115 1350.6225 66 516 527 0 (K) (T)
1381.7050 1381.6963 6.2 209 219 1 (K) (R)
1459.7398 1459.7731 -23 367 378 1 (K) (L)
1475.8150 1475.7681 32 1Met-ox 367 378 1 (K)LALDMEIHAYRK(L)
1566.7981 1566.7512 30 629 645 0 (R) (S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
935.4659	935.4798	-15		420	427	1	(R) (S)
972.5514	972.5114	41		389	397	0	(R) (S)
1032.5139	1032.5148	-0.84		182	189	0	(K) (R)
1048.5436	1048.5097	32	1Met-ox	182	189	0	(K)QLQDEMLR(R)
1089.5915	1089.5540	34		51	60	0	(R) (L)
1102.6103	1102.5744	33		378	386	1	(R) (L)
1104.6086	1104.6530	-40		42	50	1	(R) (S)
1187.6886	1187.6384	42		320	329	1	(K) (E)
1344.7264	1344.7236	2.1		49	60	1	(R) (L)
1350.7115	1350.6225	66		516	527	0	(K) (T)
1381.7050	1381.6963	6.2		209	219	1	(K) (R)
1459.7398	1459.7731	-23		367	378	1	(K) (L)
1475.8150	1475.7681	32	1Met-ox	367	378	1	(K)LALDMEIHAYRK(L)
1566.7981	1566.7512	30		629	645	0	(R) (S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 17% (114/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 11/94 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 18 (Cytokeratin 18) (K18) (CK 18)
Index: MW: 48058 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
965.4916 965.4692 23 254 261 0 (R) (K)
975.4882 975.4536 35 7 14 0 (R) (S)
1041.6229 1041.6057 17 150 158 0 (R) (L)
1065.5630 1065.5580 4.6 373 381 0 (K) (R)
1139.5871 1139.6173 -26 46 55 1 (R) (G)
1250.6251 1250.6129 9.7 pyroGlu 176 186 0 (R)QSVENDIHGLR(K)
1292.7196 1292.7214 -1.4 371 381 1 (K) (R)
1319.7246 1319.6708 41 138 149 0 (R) (I)
1419.7981 1419.7484 35 359 370 0 (R) (V)
1629.9347 1629.8852 30 pyroGlu 359 372 1 (R)QAQEYEALLNIKVK(L)
1884.0234 1884.0078 8.3 223 241 0 (K) (S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
965.4916	965.4692	23		254	261	0	(R) (K)
975.4882	975.4536	35		7	14	0	(R) (S)
1041.6229	1041.6057	17		150	158	0	(R) (L)
1065.5630	1065.5580	4.6		373	381	0	(K) (R)
1139.5871	1139.6173	-26		46	55	1	(R) (G)
1250.6251	1250.6129	9.7	pyroGlu	176	186	0	(R)QSVENDIHGLR(K)
1292.7196	1292.7214	-1.4		371	381	1	(K) (R)
1319.7246	1319.6708	41		138	149	0	(R) (I)
1419.7981	1419.7484	35		359	370	0	(R) (V)
1629.9347	1629.8852	30	pyroGlu	359	372	1	(R)QAQEYEALLNIKVK(L)
1884.0234	1884.0078	8.3		223	241	0	(K) (S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 23% (100/430AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/94 matches (11%).
Acc. #: Species: HUMAN Name: DNA-directed RNA polymerase I 135 kDa polypeptide (RNA polymerase I subunit 2) (RPA135)
Index: MW: 122140 Da pI: 8.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
941.4645 941.5169 -56 965 973 0 (R) (N)
965.4916 965.4845 7.4 711 719 0 (R) (S)
965.4916 965.5025 -11 1Met-ox 810 816 1 (K)CVRITMR(V)
1067.6044 1067.5261 73 720 728 0 (K) (I)
1104.6086 1104.5359 66 789 797 0 (K) (V)
1277.7547 1277.6968 45 pyroGlu 76 86 0 (K)QFLGYVPIMVK(S)
1307.7242 1307.8163 -70 563 574 0 (R) (E)
1319.7246 1319.6629 47 787 797 1 (K) (V)
1381.7050 1381.6893 11 868 880 0 (R) (S)
1408.7215 1408.6791 30 2Met-ox 287 296 1 (K)FYMLCLMTRK(L)
1655.8453 1655.8393 3.6 pyroGlu 731 746 0 (K)QGDSSLVFGIKPGDPR(V)
1672.8996 1672.8659 20 731 746 0 (K) (V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
941.4645	941.5169	-56		965	973	0	(R) (N)
965.4916	965.4845	7.4		711	719	0	(R) (S)
965.4916	965.5025	-11	1Met-ox	810	816	1	(K)CVRITMR(V)
1067.6044	1067.5261	73		720	728	0	(K) (I)
1104.6086	1104.5359	66		789	797	0	(K) (V)
1277.7547	1277.6968	45	pyroGlu	76	86	0	(K)QFLGYVPIMVK(S)
1307.7242	1307.8163	-70		563	574	0	(R) (E)
1319.7246	1319.6629	47		787	797	1	(K) (V)
1381.7050	1381.6893	11		868	880	0	(R) (S)
1408.7215	1408.6791	30	2Met-ox	287	296	1	(K)FYMLCLMTRK(L)
1655.8453	1655.8393	3.6	pyroGlu	731	746	0	(K)QGDSSLVFGIKPGDPR(V)
1672.8996	1672.8659	20		731	746	0	(K) (V)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 9% (107/1079AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):