MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.2.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B33
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113997
Molecular weight search (1000 - 150000 Da) selects 111891 entries.
Full pI range: 113997 entries.
Species search ( HUMAN MOUSE ) selects 13846 entries.
Combined molecular weight, pI and species searches select 13265 entries.
Pre searches select 13265 entries.

Data Set 1 Results

MS-Fit search selects 4651 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/120(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.100e+01738 (31)54.031.744.349.1 106802/5.3HUMANAlanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
21.144e+01221 (17)25.017.531.687.8 117791/5.6HUMANUbiquitin-activating enzyme E1 (A1S9 protein)
31.422e+00819 (15)22.015.824.586.1 138073/8.1HUMANEndothelial cell multimerin precursor
49.940e+00716 (13)19.013.317.284.2 127511/6.6HUMANHairless protein
55.480e+00716 (13)25.013.311.796.0 124823/6.8HUMANRetinal guanylyl cyclase 2 precursor (Guanylate cyclase 2F, retinal) (RETGC-2) (Rod outer segment membrane guanylate cyclase 2) (ROS-GC2) (Guanylate cyclase F) (GC-F)
81087x...x..x...........x.x.xx......x..xx.x...x.xx...xxx..x....xx...........x.x.x......xxxxx.x.x.....xx....x.x........x..xx.x
13559........x...........x....xx.xx........x..xxx..x....x...x.........x..........x..x...........................x...xx..x.x..
59539...............x.x.................x.........x..x.x.........x.x.x.x........x.......x...x......x.......x..x.xx.......x...
27390......x....x...x.................................xxx.......x......x...x...........x..x.......x.x..........x...x.....x...
7310....x...x...x......x..............................x...................x..x.x......x.......x....x.x.x..x........x.......x



Detailed Results

1. 38/120 matches (31%).
Acc. #: Species: HUMAN Name: Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Index: MW: 106802 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.5801972.53075114201(R) (N)
972.5801972.5842-4.24474551(K) (G)
1094.56991094.5152501Met-ox 2372460(K)SIDTGMGLER(L)
1242.73671242.7170167517621(R) (A)
1408.74801408.6749526846950(R) (V)
1421.80161421.7099656656770(K) (A)
1430.78811430.7280421541650(K) (I)
1434.80501434.6786881Met-ox 6526640(K)AEEIANEMIEAAK(A)
1482.74841482.6535642832960(K) (V)
1495.77781495.6739694334450(K) (K)
1498.74301498.6484631Met-ox 2832960(K)VGAEDADGIDMAYR(V)
1511.75511511.6688571Met-ox 4334450(K)GLVVDMDGFEEER(K)
1573.88371573.8015525855970(K) (R)
1599.85611599.7390735515641(K) (N)
1605.93071605.8641415705840(R) (V)
1634.82401634.7848249169300(K) (G)
1639.86401639.7638611Met-ox 4334461(K)GLVVDMDGFEEERK(L)
1650.87851650.7797601Met-ox 9169300(K)ASEWVQQVSGLMDGK(G)
1672.89611672.8263428999120(K) (G)
1672.89611672.8369356366501(R) (K)
1713.96441713.8884443043200(R) (G)
1729.95391729.9026305855981(K) (R)
1844.03821843.9554457307470(R) (G)
1860.08151859.9941471171320(K) (L)
1876.07651875.9890471Met-ox 1171320(K)MALELLTQEFGIPIER(L)
2053.13912053.0970217948120(R) (D)
2057.13162056.9571851Met-ox 5145310(K)MFVEEVSTGQECGVVLDK(T)
2094.16302094.0429571Met-ox 4564740(K)GAGGEDLIMLDIYAIEELR(A)
2096.06212095.9429572Met-ox 8808980(K)MHSPQTSAMLFTVDNEAGK(I)
2108.16892108.1187241Met-ox 5996160(R)RPIMSNHTATHILNFALR(S)
2133.20472133.1126439449620(K) (L)
2197.09802197.0163371Met-ox 2552720(K)MSNYDTDLFVPYFEAIQK(G)
2255.15492255.0331545325500(K) (V)
2264.29532264.2198331Met-ox 5986161(R)RRPIMSNHTATHILNFALR(S)
2298.21392298.0998505125311(R) (T)
2335.19192335.0804481331530(R) (Q)
2687.17292687.0601421Met-ox 1731940(K)DNFWEMGDTGPCGPCSEIHYDR(I)
2717.22162717.3820-598458691(K) (A)
2753.37802753.2881331Met-ox 4875100(K)YNYHLDSSGSYVFENTVATVMALR(R)
3466.86983466.7528346967290(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 54% (531/968AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 21/120 matches (17%).
Acc. #: Species: HUMAN Name: Ubiquitin-activating enzyme E1 (A1S9 protein)
Index: MW: 117791 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1250.62901250.5653518708800(R) (H)
1419.82041419.742555pyroGlu 5165260(R)QFLFRPWDVTK(L)
1436.83171436.7690445165260(R) (L)
1443.77151443.741821pyroGlu 1Met-ox 58691(R)QLYVLGHEAMKR(L)
1455.82741455.7272699749841(K) (L)
1459.77601459.6851622272390(K) (H)
1525.80191525.67718244570(K) (Q)
1573.88371573.800853100910211(K) (V)
1589.87001589.7957471Met-ox 100910211(K)ERLDQPMTEIVSR(V)
1599.85611599.765557pyroGlu 6586710(K)QPAENVNQYLTDPK(F)
1623.94081623.9070216796930(R) (S)
1655.92951655.7917834514650(R) (L)
1696.91491696.8560356366490(K) (D)
1809.01291808.9507343693840(R) (K)
1884.04121883.9463503513680(R) (A)
1994.08131994.1398-296766931(R) (S)
2384.07602384.2753-843013221(K) (F)
2600.28652600.1905375595810(R) (M)
2683.44712683.3175488078301(K) (A)
2705.23712705.4120-6598510081(K) (E)
2753.37802753.3968-6.83Met-ox 98510081(K)LEITMLSQGVSMLYSFFMPAAKLK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (273/1058AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 19/120 matches (15%).
Acc. #: Species: HUMAN Name: Endothelial cell multimerin precursor
Index: MW: 138073 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1350.75611350.6654678128221(R) (M)
1390.72531390.72520.0835745850(K) (E)
1498.74301498.664352104810600(R) (T)
1498.74301498.69263425370(K) (T)
1617.87771617.762171pyroGlu 4854980(K)QTHLEGALEQEHSR(S)
1634.82401634.7307578238350(K) (K)
1634.82401634.7887224854980(K) (S)
1650.87851650.7256931Met-ox 8238350(K)MYQMFNETTSQVR(K)
1762.87471762.8257288238361(K) (Y)
1789.91201789.9158-2.1pyroGlu 6616751(R)QTMTYEQPKEAIVIR(K)
1789.91201790.0329-677968111(R) (D)
1794.93721794.8155682Met-ox 8238361(K)MYQMFNETTSQVRK(Y)
1794.93721794.8696387167301(R) (T)
1811.98081811.9002453974110(K) (T)
1876.07651875.9850495745891(K) (G)
2057.13162057.0411442Met-ox 3243401(K)MTDQVNYQAMKLTLLQK(K)
2126.10812126.0051481Met-ox 7657810(K)CTSDMETILTFIPQFHR(L)
2233.22662233.1213474804981(K) (S)
2298.21392298.165821114911690(K) (L)
2344.05432344.1647-473934111(R) (T)
2384.07602384.1300-233Met-ox 5555731(K)KQSLMMLQMFEDLHIQESK(I)
2501.26862501.3768-43118612071(R) (G)
2717.22162717.3245-38106910921(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (271/1228AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 16/120 matches (13%).
Acc. #: Species: HUMAN Name: Hairless protein
Index: MW: 127511 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1234.72941234.7384-7.38138251(K) (K)
1281.66981281.6261343143240(R) (G)
1281.66981281.7279-458688790(R) (T)
1350.75611350.6878512402510(R) (D)
1639.86401639.7928435835980(R) (C)
1639.86401639.8226254134291(K) (R)
1650.87851650.8532151952091(K) (D)
1655.92951655.8175681Met-ox 4134291(K)RAGSPEVQGAMGSPAPK(R)
1729.95391729.8509606987131(R) (T)
1811.98081812.0595-437938081(R) (I)
1838.00331837.9271417767920(R) (I)
2053.13912052.9822761011180(K) (C)
2096.06212096.0850-119509680(R) (L)
2225.19502225.17867.47487681(R) (L)
2252.13622252.0366447097281(K) (T)
2383.04602383.1514-441Met-ox 991181(R)WKEAMLTHPLAFCGPACPPR(C)
2565.39572565.2698495755981(R) (C)
2717.22162717.3656-537697921(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (235/1189AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 16/120 matches (13%).
Acc. #: Species: HUMAN Name: Retinal guanylyl cyclase 2 precursor (Guanylate cyclase 2F, retinal) (RETGC-2) (Rod outer segment membrane guanylate cyclase 2) (ROS-GC2) (Guanylate cyclase F) (GC-F)
Index: MW: 124823 Da pI: 6.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1094.56991094.6111-38AcetN 1101(-)FLGLGRFSR(L)
1250.62901250.7414-9011201(R) (L)
1300.63891300.6030285895991(K) (D)
1408.74801408.74760.28105310641(K) (G)
1408.74801408.8276-5670821(K) (I)
1650.87851650.8413239289430(K) (R)
1838.00331838.0138-5.71Met-ox 6426561(K)LDWMFKSSLLLDLIK(G)
1860.08151860.0529159819990(R) (Y)
1876.07651876.0479151Met-ox 9819990(R)IGLHSGPVVAGVVGLTMPR(Y)
2053.13912053.0090633964140(R) (E)
2197.09802197.1399-192112311(R) (A)
2252.13622252.12734.06296471(R) (S)
2264.29532264.1241761Met-ox 7477651(R)GTPFCMMDLPAQEIINRLK(K)
2282.22982282.0473804154320(K) (F)
2298.21392298.0422751Met-ox 4154320(K)EWELHSTYTVDMEMELLR(F)
2600.28652600.3936-41102210440(R) (G)
3466.86983466.7141453Met-ox 2422701(R)IRIIIMCMHSALIGGETQMHLLECAHDLK(M)
3466.86983466.7397381Met-ox 2712991(K)MTDGTYVFVPYDALLYSLPYKHTPYQVLR(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (277/1108AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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