MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B34
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 200000 Da) selects 113421 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 13705 entries.
Pre searches select 13705 entries.

Data Set 1 Results

MS-Fit search selects 3515 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/120(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.796e+00622 (18)15.018.3-0.43148.4 180068/6.4HUMANHypothetical protein KIAA0373
22.383e+00610 (8)9.08.3-16.745.1 145203/5.5HUMAN130 kDa leucine-rich protein (LRP 130) (GP130) (Leucine-rich PPR-motif containing protein)
31.340e+00612 (10)15.010.08.1260.6 107067/5.7MOUSECoatomer beta subunit (Beta-coat protein) (Beta-COP)
45.081e+00510 (8)15.08.36.3062.7 107140/5.6HUMANCoatomer beta subunit (Beta-coat protein) (Beta-COP)
53.642e+00514 (11)19.011.78.1542.5 72752/4.8HUMANGPI-anchored protein p137 (p137GPI)
83572...................x...x...........x.....x.x..xxx.....x.x..xx...x...xx.....xx......x.x.................xx......x........
104737......x..............x..x...x...x...............................................x...x...x.x...........................x.
66139...............................xx.......x...................x........x.....x...x...xx...x.........x...x.................
57990........................................x.........x.........x........x.........x...xx...x.........x...x.................
70951..x.........x.....................x............xx.x..x.x..x....x........x.x..............................x.........x....



Detailed Results

1. 22/120 matches (18%).
Acc. #: Species: HUMAN Name: Hypothetical protein KIAA0373
Index: MW: 180068 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
994.5139994.5209-7.17527590(K) (E)
1013.53311013.5056273063130(K) (L)
1130.57501130.5918-157077151(K) (E)
1179.60701179.568033941041(K) (K)
1217.65121217.6378116486580(R) (F)
1251.68141251.7286-383223311(K) (R)
1267.65181267.65090.686596681(K) (Q)
1283.65911283.6458101Met-ox 6596681(K)FKQTAWDLMK(Q)
1334.62521334.6487-187737841(K) (N)
1350.69871350.6436411Met-ox 7737841(K)EANSRAPTTTMR(N)
1404.68161404.7269-321Met-ox 7787891(R)APTTTMRNLVER(L)
1417.74371417.74260.76103910501(R) (K)
1458.73501458.7705-24113711481(K) (L)
1479.73601479.7187123733841(R) (L)
1481.76441481.7236287307410(K) (K)
1567.83411567.8419-4.94044151(K) (L)
1583.80811583.8368-181Met-ox 4044151(K)VINWHMKIEELR(L)
1778.92331778.91763.27457591(K) (E)
1778.92331778.9441-123333481(R) (T)
1785.93661785.9598-138468601(R) (L)
2091.93082092.0232-44121412321(K) (L)
2097.06412097.05384.91Met-ox 2452621(R)MQLLDYQAQSDEKSLIAK(L)
2282.27342282.171645105310711(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (238/1539AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/120 matches (8%).
Acc. #: Species: HUMAN Name: 130 kDa leucine-rich protein (LRP 130) (GP130) (Leucine-rich PPR-motif containing protein)
Index: MW: 145203 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
888.3982888.4328-398468520(K) (A)
1000.46271000.4749-12170(-) (S)
1000.46271000.4774-151Met-ox 1942020(K)AGYLSMSQK(F)
1016.44051016.4698-291Met-ox 170(-)MPCFYLR(S)
1045.56681045.5682-1.4121812260(K) (N)
1092.56891092.5478194854920(R) (M)
1715.85801715.9161-34108911020(K) (K)
1782.92981783.0039-422292440(K) (E)
1792.89361792.9445-286196350(R) (V)
1835.88621835.8412259009160(K) (D)
2498.19882498.2688-282782980(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (125/1273AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/120 matches (10%).
Acc. #: Species: MOUSE Name: Coatomer beta subunit (Beta-coat protein) (Beta-COP)
Index: MW: 107067 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1091.57081091.540728pyroGlu 1Met-ox 6376460(R)QSLSQMLSAK(L)
1092.56891092.5723-3.16376460(R) (L)
1173.61501173.5752346036120(K) (I)
1417.74371417.7877-311Met-ox 4424531(K)MLEVFHAIKSVK(I)
1481.76441481.8038-271291410(K) (A)
1567.83411567.7572491Met-ox 6346461(K)ECRQSLSQMLSAK(L)
1707.79731707.7574233673801(K) (Q)
1778.92331778.8358498838980(K) (S)
1782.92981782.92512.61081221(K) (F)
1792.89361792.9697-422622790(K) (A)
1953.02851953.01815.44965130(K) (L)
2087.12642087.10659.65445630(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (149/953AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/120 matches (8%).
Acc. #: Species: HUMAN Name: Coatomer beta subunit (Beta-coat protein) (Beta-COP)
Index: MW: 107140 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1173.61501173.5752346036120(K) (I)
1320.69861320.6469394704800(K) (R)
1417.74371417.7877-311Met-ox 4424531(K)MLEVFHAIKSVK(I)
1481.76441481.8038-271291410(K) (A)
1707.79731707.7574233673801(K) (Q)
1778.92331778.8358498838980(K) (S)
1782.92981782.92512.61081221(K) (F)
1792.89361792.9697-422622790(K) (A)
1953.02851953.01815.44965130(K) (L)
2087.12642087.10659.65445630(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (147/953AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/120 matches (11%).
Acc. #: Species: HUMAN Name: GPI-anchored protein p137 (p137GPI)
Index: MW: 72752 Da pI: 4.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
868.5429868.514433pyroGlu 3100(K)QILGVIDK(K)
938.4145938.4219-7.922280(K) (M)
1123.54241123.53843.620281(K) (M)
1267.65181267.63561362720(R) (T)
1283.65911283.6306221Met-ox 62720(R)SFMALSQDIQK(T)
1320.69861320.7415-32951050(K) (L)
1330.65811330.6789-161391491(K) (L)
1346.68281346.67386.71Met-ox 1391491(K)LVDPERDMSLR(L)
1390.65831390.6637-3.981911(R) (R)
1455.72401455.71208.346570(K) (E)
1519.71481519.6739271Met-ox 2452570(R)QFMAETQFTSGEK(E)
1527.80151527.7304475475591(R) (G)
2105.16372105.113024951121(K) (T)
2295.11842295.1410-9.91501680(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (125/649AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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