MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B35
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (10000 - 150000 Da) selects 103782 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 13235 entries.
Pre searches select 13235 entries.

Data Set 1 Results

MS-Fit search selects 621 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/41(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
114238 (19)9.019.527.478.1 142808/6.6HUMANBreakpoint cluster region protein
25975 (12)9.012.226.8133 89638/5.8HUMANProtein-glutamine gamma-glutamyltransferase K (Transglutaminase K) (TGase K) (TGK) (TG(K)) (Transglutaminase 1) (Epidermal TGase)
34446 (14)14.014.647.829.6 52905/7.5HUMAN26S proteasome non-ATPase regulatory subunit 12 (26S proteasome regulatory subunit p55)
44025 (12)12.012.237.253.8 50419/4.8MOUSETubulin beta-3
54025 (12)12.012.237.253.8 50433/4.8HUMANTubulin beta-4 chain (Tubulin beta-III)
64019 (21)12.022.019.7101 102932/6.3HUMANThyroid peroxidase precursor (TPO)
73915 (12)31.012.228.553.4 15003/8.9HUMANProtachykinin 1 precursor (PPT) [Contains: Substance P; Neurokinin A (NKA) (Substance K) (Neuromedin L); Neuropeptide K (NPK); Neuropeptide gamma; C-terminal flanking peptide]
83877 (17)10.017.132.695.9 97287/6.7MOUSEGlycogen phosphorylase, muscle form (Myophosphorylase)
93705 (12)9.012.219.784.2 119500/7.5MOUSERetinoblastoma-like protein 1 (107 kDa retinoblastoma-associated protein) (PRB1) (P107)
103267 (17)8.017.1-0.461107 111659/7.9HUMANDynamin-like 120 kDa protein, mitochondrial precursor (Optic atrophy 1 gene protein)
43469x...x...........xx.........x.x........x.x
17269..x.............x.........x.....x.......x
52830..x..x.x.........................x.x....x
32254....................xx.........xx..x.....
39595....................xx.........xx..x.....
84454........x...x...x...xx...xxx..........x..
61821.......x............xx.....x.......x.....
19232.......x......x.......xx..x..x.......x...
37023............x.............xx.........x..x
73483........xx..........x.x.........x...x.x..


Detailed Results

1. 8/41 matches (19%).
Acc. #: Species: HUMAN Name: Breakpoint cluster region protein
Index: MW: 142808 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
961.5914961.58358.2106510721(R) (Q)
1007.52601007.51629.83533610(R) (M)
1174.67111174.650618101810280(R) (L)
1179.65641179.5606812993081(R) (L)
1179.65641179.637416126212711(K) (-)
1636.81741636.8845-413944080(R) (K)
1681.02051680.9006711261421(K) (D)
2211.23052211.196016119812170(K) (E)
2535.41362535.2410688688881(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (121/1271AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/41 matches (12%).
Acc. #: Species: HUMAN Name: Protein-glutamine gamma-glutamyltransferase K (Transglutaminase K) (TGase K) (TGK) (TG(K)) (Transglutaminase 1) (Epidermal TGase)
Index: MW: 89638 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.6132972.5227932002080(K) (V)
1174.67111174.581676941060(R) (G)
1609.80661609.9165-681631770(R) (G)
1707.87241707.7978442262400(R) (N)
2535.41362535.4411-117157371(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (75/817AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/41 matches (14%).
Acc. #: Species: HUMAN Name: 26S proteasome non-ATPase regulatory subunit 12 (26S proteasome regulatory subunit p55)
Index: MW: 52905 Da pI: 7.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.6132972.584230951031(R) (M)
1045.58591045.5505343653721(R) (I)
1054.55701054.48396956650(R) (I)
1717.97811717.88355579920(K) (R)
1874.08731873.98465579931(K) (R)
2535.41362535.3016443784000(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (65/456AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/41 matches (12%).
Acc. #: Species: MOUSE Name: Tubulin beta-3
Index: MW: 50419 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1319.74661319.7033331631740(R) (V)
1335.74541335.6982351Met-ox 1631740(R)IMNTFSVVPSPK(V)
1696.88831696.8335323373500(K) (V)
1707.87241707.86275.71Met-ox 2832970(R)ALTVPELTQQMFDAK(N)
1874.08731873.9370803633790(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (58/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/41 matches (12%).
Acc. #: Species: HUMAN Name: Tubulin beta-4 chain (Tubulin beta-III)
Index: MW: 50433 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1319.74661319.7033331631740(R) (V)
1335.74541335.6982351Met-ox 1631740(R)IMNTFSVVPSPK(V)
1696.88831696.8335323373500(K) (V)
1707.87241707.86275.71Met-ox 2832970(R)ALTVPELTQQMFDAK(N)
1874.08731873.9370803633790(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (58/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/41 matches (21%).
Acc. #: Species: HUMAN Name: Thyroid peroxidase precursor (TPO)
Index: MW: 102932 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.62481066.5832397627691(R) (H)
1133.58781133.6166-2539481(R) (L)
1174.67111174.6003608378461(R) (A)
1319.74661319.8163-534404510(K) (D)
1335.74541335.6592651511610(K) (D)
1593.82501593.8497-151Met-ox 6496621(R)TGPLFACLIGKQMK(A)
1609.80661609.8563-313423551(R) (L)
1636.81741636.6873799099240(R) (D)
2211.23052211.1006592072251(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (114/933AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/41 matches (12%).
Acc. #: Species: HUMAN Name: Protachykinin 1 precursor (PPT) [Contains: Substance P; Neurokinin A (NKA) (Substance K) (Neuromedin L); Neuropeptide K (NPK); Neuropeptide gamma; C-terminal flanking peptide]
Index: MW: 15003 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1054.55701054.5243311001090(K) (R)
1319.74661319.678252981091(R) (R)
1335.74541335.6731541Met-ox 981091(R)HKTDSFVGLMGK(R)
1636.81741636.8335-9.942540(K) (I)
1874.08731874.05871555701(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (41/129AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/41 matches (17%).
Acc. #: Species: MOUSE Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97287 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1054.55701054.4846693253330(R) (V)
1145.69401145.5890925946021(R) (T)
1355.64971355.6748-1951610(R) (D)
1475.83641475.7535563603711(R) (T)
1609.80661609.8702-395565690(K) (R)
1681.02051680.9325526436561(R) (V)
2161.16082161.1235174394581(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (89/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/41 matches (12%).
Acc. #: Species: MOUSE Name: Retinoblastoma-like protein 1 (107 kDa retinoblastoma-associated protein) (PRB1) (P107)
Index: MW: 119500 Da pI: 7.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1133.58781133.5955-6.83643740(R) (R)
1609.80661609.8742-422652771(K) (L)
1636.81741636.81173.46346470(R) (Y)
2161.16082161.0496511Met-ox 5005170(K)SLMACCLEIVLFAYSSPR(T)
2161.16082161.0712413453621(K) (R)
2535.41362535.2342719449650(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (96/1063AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/41 matches (17%).
Acc. #: Species: HUMAN Name: Dynamin-like 120 kDa protein, mitochondrial precursor (Optic atrophy 1 gene protein)
Index: MW: 111659 Da pI: 7.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.62481066.5209975725790(R) (N)
1071.53861071.5435-4.5pyroGlu 8969040(R)QQLTNTEVR(R)
1071.53861071.6162-729329401(K) (K)
1319.74661319.68604639490(R) (L)
1355.64971355.7112-4575851(K) (R)
1707.87241707.9281-332152280(K) (Y)
1959.96061959.94866.17567721(R) (K)
2211.23052211.22631.939561(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (86/960AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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