MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B36
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (10000 - 150000 Da) selects 103782 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 13235 entries.
Pre searches select 13235 entries.

Data Set 1 Results

MS-Fit search selects 201 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/48(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.082e+00511 (22)17.022.93.4043.1 123800/5.5HUMANVinculin (Metavinculin)
22.483e+0049 (18)14.018.84.1543.0 116806/5.7MOUSEVinculin (Metavinculin)
31465 (10)7.010.4-9.5870.6 88055/5.7HUMANIntegrin beta-5 precursor
41458 (16)26.016.7-17.255.8 23633/4.6MOUSENeuromodulin (Axonal membrane protein GAP-43) (PP46) (B-50) (Protein F1) (Calmodulin-binding protein P-57)
51234 (8)11.08.3-16.974.6 39180/10.0HUMANMelanoma-associated antigen B3 (MAGE-B3 antigen)
87413...................xx........x..x..x.....xxx.xxx
68183...................xx........x...........xxx.xxx
115933.......xx.................x......x...........x..
108948......x..x.....x..xx.....x......xx..............
39801......x...x......................x..........x...



Detailed Results

1. 11/48 matches (22%).
Acc. #: Species: HUMAN Name: Vinculin (Metavinculin)
Index: MW: 123800 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1021.45941021.4478118828890(K) (A)
1025.46131025.4760-142Met-ox 1711781(K)MAKMIDER(Q)
1149.58411149.6380-47111611251(K) (W)
1170.61931170.6483-256706800(R) (I)
1457.79661457.79640.133533660(K) (V)
1983.02401982.9684282823001(K) (Q)
1983.02401983.00489.75035201(R) (G)
2036.09272036.0552181141320(R) (K)
2076.21482076.18171636560(K) (V)
2381.24222381.2101136086291(K) (A)
2447.22752447.1883168338530(R) (L)
2508.24652508.2119143874091(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (194/1134AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/48 matches (18%).
Acc. #: Species: MOUSE Name: Vinculin (Metavinculin)
Index: MW: 116806 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1021.45941021.4478118828890(K) (A)
1025.46131025.4760-142Met-ox 1711781(K)MAKMIDER(Q)
1149.58411149.6380-47104810571(K) (W)
1983.02401983.00489.75035201(R) (G)
2036.09272036.0552181141320(R) (K)
2076.21482076.18171636560(K) (V)
2381.24222381.2101136086291(K) (A)
2447.22752447.1883168338530(R) (L)
2508.24652508.2119143874091(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (150/1066AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/48 matches (10%).
Acc. #: Species: HUMAN Name: Integrin beta-5 precursor
Index: MW: 88055 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
968.5106968.4664463403460(K) (N)
969.5071969.50462.71771850(R) (D)
1094.48391094.5118-262272350(R) (K)
1217.53961217.5948-455155250(R) (G)
2381.24222381.3024-251211(-) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (57/799AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/48 matches (16%).
Acc. #: Species: MOUSE Name: Neuromodulin (Axonal membrane protein GAP-43) (PP46) (B-50) (Protein F1) (Calmodulin-binding protein P-57)
Index: MW: 23633 Da pI: 4.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
965.4327965.4483-16AcetN 171(-)LCCMRR(T)
981.4034981.4432-41AcetN 1Met-ox 171(-)LCCMRR(T)
1007.42991007.4645-341371460(K) (A)
1019.43671019.4758-382092171(K) (E)
1021.45941021.4802-201261360(K) (A)
1089.45511089.4700-141151250(K) (A)
1170.61931170.5643471471571(K) (Q)
1217.53961217.5650-211141251(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (60/227AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/48 matches (8%).
Acc. #: Species: HUMAN Name: Melanoma-associated antigen B3 (MAGE-B3 antigen)
Index: MW: 39180 Da pI: 10.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
965.4327965.4805-49pyroGlu 2622700(R)QVPNSNPAR(Y)
982.5060982.5070-1.02622700(R) (Y)
1217.53961217.5948-451841940(K) (G)
2211.14932211.08662816351(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (40/346AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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