MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B47
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1465 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/100(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.762e+00710 (10)24.010.027.232.6 70872/5.4MOUSEHeat shock cognate 71 kDa protein
21.762e+00710 (10)24.010.027.232.6 70899/5.4HUMANHeat shock cognate 71 kDa protein
35.780e+00510 (10)27.010.02.5863.1 61050/5.7HUMANProlyl 4-hydroxylase alpha-1 subunit precursor (4-PH alpha-1) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-1 subunit)
44.271e+00514 (14)32.014.0-9.2752.8 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
53.049e+0047 (7)9.07.016.445.3 86491/6.4HUMANAMP deaminase 1 (Myoadenylate deaminase) (AMP deaminase isoform M)
62.025e+0047 (7)16.07.04.7162.7 57118/6.1MOUSEAspartyl-tRNA synthetase (Aspartate--tRNA ligase) (AspRS)
72.024e+0047 (7)16.07.04.7162.7 57137/6.1HUMANAspartyl-tRNA synthetase (Aspartate--tRNA ligase) (AspRS)
81.603e+0047 (7)16.07.01.7858.0 57827/5.9HUMANAlkaline phosphatase, placental type 3 precursor
91.594e+0046 (6)17.06.027.725.3 70696/5.8MOUSEHeat shock 70 kDa-like protein 1
101.546e+0046 (6)15.06.014.238.1 57458/5.5MOUSEGlycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK)
98245.......x..x............x.............xx..x..................x.........x...........x...............x.
96727.......x..x............x.............xx..x..................x.........x...........x...............x.
890......x..x...............x.xx.x.........x.................................x..........x..x...........
84429.........x...x................x..x...x........xx...xx...x.x.....x........x...............x..........
77984.........................x......x.............xx.....x.......x...........x..........................
60365..............xx..............................xx........x...........x...............x...............
42499..............xx..............................xx........x...........x...............x...............
8783.x......x..x.............x...........x.......................x..........................x...........
22079.......x.............x.x...........................................................x..............xx
2240..................................x..x........x............x.....x........................x.........


Detailed Results

1. 10/100 matches (10%).
Acc. #: Species: MOUSE Name: Heat shock cognate 71 kDa protein
Index: MW: 70872 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1197.70781197.6632374594690(K) (G)
1253.66371253.6166383023110(R) (G)
1487.73181487.70182037490(R) (L)
1649.84611649.79573157710(K) (R)
1653.82771653.8324-2.8891020(K) (V)
1691.79511691.7261412212360(K) (M)
1875.93001875.92363.41131281(K) (E)
1982.04931981.9983261381550(K) (Q)
2277.20502277.1338314250(K) (V)
2774.46222774.3273494244470(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (159/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/100 matches (10%).
Acc. #: Species: HUMAN Name: Heat shock cognate 71 kDa protein
Index: MW: 70899 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1197.70781197.6632374594690(K) (G)
1253.66371253.6166383023110(R) (G)
1487.73181487.70182037490(R) (L)
1649.84611649.79573157710(K) (R)
1653.82771653.8324-2.8891020(K) (V)
1691.79511691.7261412212360(K) (M)
1875.93001875.92363.41131281(K) (E)
1982.04931981.9983261381550(K) (Q)
2277.20502277.1338314250(K) (V)
2774.46222774.3273494244470(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (159/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/100 matches (10%).
Acc. #: Species: HUMAN Name: Prolyl 4-hydroxylase alpha-1 subunit precursor (4-PH alpha-1) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-1 subunit)
Index: MW: 61050 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.60931179.6308-185005100(R) (W)
1249.59951249.6541-442312400(K) (A)
1513.80451513.74404073860(K) (L)
1530.70531530.7150-6.31601720(K) (V)
1564.84381564.7760433833960(K) (I)
1567.78791567.78690.65pyroGlu 3283391(K)QEDEWDKPRIIR(F)
1687.96681687.890745911040(R) (D)
2043.07002043.06233.83623791(R) (I)
2292.09292292.1723-351371571(R) (H)
2369.11062369.1202-4.14794991(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (148/534AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/100 matches (14%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1249.59951249.5734211Met-ox 2712810(K)GEENLMDAQVK(A)
1350.72051350.7568-273083181(K) (W)
1567.78791567.8444-363653781(K) (G)
1629.86551629.8812-9.64514661(R) (L)
1649.84611649.9201-45AcetN 1161(-)ALHVPKAPGFAQMLK(D)
1738.91371738.9501-211161(-) (D)
1754.89811754.9450-271Met-ox 1161(-)MALHVPKAPGFAQMLK(D)
1779.94261779.9162151561711(R) (Q)
1789.96111790.0329-405055201(K) (V)
1838.95521838.9574-1.21211370(R) (K)
1851.98811851.90234638541(K) (M)
1894.99991895.0101-5.42072240(K) (K)
2036.03602036.00241721371(K) (E)
2384.07192384.1114-172612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (179/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/100 matches (7%).
Acc. #: Species: HUMAN Name: AMP deaminase 1 (Myoadenylate deaminase) (AMP deaminase isoform M)
Index: MW: 86491 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1513.80451513.7651261972090(R) (M)
1589.88621589.8188424194311(K) (S)
1738.91371738.8726246596730(K) (L)
1754.89811754.8675171Met-ox 6596730(K)EPLMEEYAIAAQVFK(L)
1796.82301796.8762-304484610(R) (I)
1879.89971879.88269.17007160(K) (R)
2036.03602035.9837267007171(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (73/747AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/100 matches (7%).
Acc. #: Species: MOUSE Name: Aspartyl-tRNA synthetase (Aspartate--tRNA ligase) (AspRS)
Index: MW: 57118 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1399.77981399.7884-6.14764870(R) (Q)
1415.72031415.7833-451Met-ox 4764870(R)VTMLFLGLHNVR(Q)
1738.91371738.84344029430(R) (V)
1754.89811754.8383341Met-ox 29430(R)YGISSMIQSQEKPDR(V)
1838.95521838.9264161Met-ox 3944080(K)YPLAVRPFYTMPDPR(N)
1939.94991939.9990-2582990(R) (Q)
2288.20052288.1576194544751(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (82/501AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/100 matches (7%).
Acc. #: Species: HUMAN Name: Aspartyl-tRNA synthetase (Aspartate--tRNA ligase) (AspRS)
Index: MW: 57137 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1399.77981399.7884-6.14764870(R) (Q)
1415.72031415.7833-451Met-ox 4764870(R)VTMLFLGLHNVR(Q)
1738.91371738.84344029430(R) (V)
1754.89811754.8383341Met-ox 29430(R)YGISSMIQSQEKPDR(V)
1838.95521838.9264161Met-ox 3944080(K)YPLAVRPFYTMPDPR(N)
1939.94991939.9990-2582990(R) (Q)
2288.20052288.1576194544751(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (82/501AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/100 matches (7%).
Acc. #: Species: HUMAN Name: Alkaline phosphatase, placental type 3 precursor
Index: MW: 57827 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
927.5070927.5264-2148560(K) (N)
1211.69031211.64583785950(K) (F)
1305.74861305.6915441271390(K) (F)
1513.80451513.8379-222502621(R) (H)
1649.84611649.8876-252542671(K) (Y)
1879.89971879.8781113033190(R) (L)
2369.11062369.1373-114434640(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (90/535AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/100 matches (6%).
Acc. #: Species: MOUSE Name: Heat shock 70 kDa-like protein 1
Index: MW: 70696 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1197.70781197.6955101621730(K) (I)
1475.78051475.7238386006110(K) (L)
1487.73181487.70182039510(R) (L)
2283.27302283.1733441741951(R) (H)
2774.46222774.4035213603861(R) (S)
2786.45492786.3637334264490(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (110/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 6/100 matches (6%).
Acc. #: Species: MOUSE Name: Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK)
Index: MW: 57458 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1633.83961633.8048212052170(R) (E)
1649.84611649.7998281Met-ox 2052170(R)TMLFNIHSLEWDK(E)
1738.91371738.8838172933070(K) (L)
1860.01091859.99011171861(K) (A)
1921.99811921.9417293904050(K) (E)
2418.29732418.3509-223083301(K) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (83/524AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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