MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B49
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 920 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/47(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.075e+00817 (36)33.036.2-17.252.7 70872/5.4MOUSEHeat shock cognate 71 kDa protein
24.073e+00817 (36)33.036.2-17.252.7 70899/5.4HUMANHeat shock cognate 71 kDa protein
31.325e+00615 (31)26.031.9-39.188.4 75874/5.4HUMANAnnexin VI (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II)
42.553e+0059 (19)20.019.1-32.544.3 70053/5.5HUMANHeat shock 70 kDa protein 1 (HSP70.1) (HSP70-1/HSP70-2)
58.089e+0049 (19)22.019.1-6.4788.5 70854/5.7HUMANHEAT SHOCK 70 KD PROTEIN 6 (HEAT SHOCK 70 KD PROTEIN B')
67.358e+0046 (12)36.012.8-15.686.8 26907/7.0HUMANHeat shock 70 kDa protein 7 (Heat shock 70 kDa protein B)
75.639e+0048 (17)21.017.0-23.150.1 69995/5.5MOUSEHeat shock 70 kDa protein 1 (HSP70.1) (HSP70-1/HSP70-2)
82.700e+00412 (25)30.025.5-19.751.8 53675/5.5HUMANKeratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
92.525e+0048 (17)19.017.0-34.967.8 70696/5.8MOUSEHeat shock 70 kDa-like protein 1
1088688 (17)20.017.0-37.1137 50538/5.9MOUSERAB GDP dissociation inhibitor beta-2 (RAB GDI beta-2) (GDI-3)
101395.........xx.xxx...x.....x...xxx..x..x.x.x.x.x.x
99823.........xx.xxx...x.....x...xxx..x..x.x.x.x.x.x
21935x.xxxx.x....x..x......x...x.x.x..x.x..x........
67657......x..x..x........x...x....x...xx..........x
22083............x....x.....x......x...xx......x..xx
96582............x....x............x.x.x..........x.
54635......x.xx..x.................x...xx..........x
28336.xxx...x....xx.x...x..x..xx.x..................
22768.........x..x............xx.x.x...x...........x
108492x.....x.x....x.....x....x..................x.x.



Detailed Results

1. 17/47 matches (36%).
Acc. #: Species: MOUSE Name: Heat shock cognate 71 kDa protein
Index: MW: 70872 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1197.63281197.6632-254594690(K) (G)
1199.63231199.6748-351601710(K) (I)
1228.58861228.6286-3326360(K) (T)
1251.58691251.6196-261Met-ox 2372460(R)MVNHFIAEFK(R)
1253.58001253.6166-293023110(R) (G)
1319.60151319.59425.51Met-ox 5405500(K)NSLESYAFNMK(A)
1450.66901450.8058-943463571(K) (E)
1480.74851480.7549-4.33003111(R) (G)
1481.75551481.8076-353293420(K) (I)
1487.67781487.7018-1637490(R) (L)
1669.80451669.8273-141Met-ox 891020(K)HWPFMVVNDAGRPK(V)
1691.71231691.7261-8.22212360(K) (M)
1745.81791745.80954.85845971(K) (E)
1837.98541838.0136-153263421(K) (I)
1981.99401981.9983-2.21381550(K) (Q)
2277.16332277.1338134250(K) (V)
2774.38922774.3273224244470(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (217/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/47 matches (36%).
Acc. #: Species: HUMAN Name: Heat shock cognate 71 kDa protein
Index: MW: 70899 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1197.63281197.6632-254594690(K) (G)
1199.63231199.6748-351601710(K) (I)
1228.58861228.6286-3326360(K) (T)
1251.58691251.6196-261Met-ox 2372460(R)MVNHFIAEFK(R)
1253.58001253.6166-293023110(R) (G)
1319.60151319.59425.51Met-ox 5405500(K)NSLESYAFNMK(A)
1450.66901450.8058-943463571(K) (E)
1480.74851480.7549-4.33003111(R) (G)
1481.75551481.8076-353293420(K) (I)
1487.67781487.7018-1637490(R) (L)
1669.80451669.8273-141Met-ox 891020(K)HWPFMVVNDAGRPK(V)
1691.71231691.7261-8.22212360(K) (M)
1745.81791745.80954.85845971(K) (E)
1837.98541838.0136-153263421(K) (I)
1981.99401981.9983-2.21381550(K) (Q)
2277.16332277.1338134250(K) (V)
2774.38922774.3273224244470(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (217/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 15/47 matches (31%).
Acc. #: Species: HUMAN Name: Annexin VI (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II)
Index: MW: 75874 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
999.5671999.6203-534574650(K) (T)
1066.52861066.6049-725615681(R) (M)
1079.48741079.5890-945996070(K) (L)
1088.55681088.6138-521141220(K) (T)
1099.46291099.5060-392512590(R) (L)
1129.57731129.6767-881571660(K) (E)
1228.58861228.7014-926306391(R) (E)
1277.66141277.6451131Met-ox 2973061(K)YEKSLYSMIK(N)
1419.71661419.70557.84074181(K) (S)
1475.72721475.7593-221001131(K) (C)
1480.74851480.7072284724831(R) (S)
1487.67781487.7535-512512621(R) (A)
1487.67781487.8182-9441531(K) (Q)
1669.80451669.8810-464284420(R) (Q)
1687.86781687.8040383553700(K) (A)
1745.81791745.8346-9.61411560(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (175/673AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/47 matches (19%).
Acc. #: Species: HUMAN Name: Heat shock 70 kDa protein 1 (HSP70.1) (HSP70-1/HSP70-2)
Index: MW: 70053 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1109.51231109.5744-563493570(K) (D)
1197.63281197.6955-521601710(K) (I)
1228.58861228.6286-3326360(K) (T)
1417.70731417.7592-372372471(R) (K)
1465.71531465.8127-663293420(K) (I)
1487.67781487.7018-1637490(R) (L)
1675.71941675.7312-7.12212360(K) (L)
1687.86781687.9019-201721870(R) (T)
2774.38922774.4035-5.13583841(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (129/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/47 matches (19%).
Acc. #: Species: HUMAN Name: HEAT SHOCK 70 KD PROTEIN 6 (HEAT SHOCK 70 KD PROTEIN B')
Index: MW: 70854 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1228.58861228.6286-3328380(R) (T)
1305.64701305.6691-171311420(K) (H)
1439.77161439.654282pyroGlu 6306430(R)QGDPSTGPIIEEVD(-)
1487.67781487.7018-1639510(R) (L)
1675.71941675.7312-7.12232380(K) (L)
1687.86781687.9019-201741890(R) (R)
1981.99401981.9231361Met-ox 80951(K)FADTTVQSDMKHWPFR(V)
2384.00712384.1920-786270(R) (V)
2774.38922774.4035-5.13603861(K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (147/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/47 matches (12%).
Acc. #: Species: HUMAN Name: Heat shock 70 kDa protein 7 (Heat shock 70 kDa protein B)
Index: MW: 26907 Da pI: 7.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1228.58861228.6286-3328380(R) (T)
1305.64701305.6691-171311420(K) (H)
1487.67781487.7018-1639510(R) (L)
1639.91211639.81925759730(K) (R)
1675.71941675.7312-7.12232380(K) (L)
2384.00712384.1920-786270(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (89/247AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/47 matches (17%).
Acc. #: Species: MOUSE Name: Heat shock 70 kDa protein 1 (HSP70.1) (HSP70-1/HSP70-2)
Index: MW: 69995 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1109.51231109.5744-563493570(K) (D)
1179.56441179.6122-401031121(K) (S)
1197.63281197.6955-521601710(K) (I)
1228.58861228.6286-3326360(K) (T)
1487.67781487.7018-1637490(R) (L)
1675.71941675.7312-7.12212360(K) (L)
1687.86781687.9019-201721870(R) (T)
2774.38922774.3273224244470(K) (A)
2774.38922774.4035-5.13583841(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (138/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 12/47 matches (25%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1045.50781045.5530-431501580(R) (L)
1066.52861066.5169111791861(K) (T)
1079.48741079.5122-232652730(K) (S)
1079.48741079.5373-462262330(R) (E)
1129.57731129.6217-393423521(R) (L)
1129.57731129.6217-393533620(K) (A)
1228.58861228.5520301Met-ox 1982071(K)KDVDEAYMNK(V)
1251.58691251.6446-4612231(K) (S)
1277.66141277.7105-383823920(K) (K)
1344.63871344.6759-283293410(R) (G)
1419.71661419.7484-222142250(R) (Q)
1465.71531465.70695.71Met-ox 3053161(K)TEISEMNRNISR(L)
1475.72721475.6987194024140(R) (T)
1480.74851480.7721-161871981(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (149/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/47 matches (17%).
Acc. #: Species: MOUSE Name: Heat shock 70 kDa-like protein 1
Index: MW: 70696 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1197.63281197.6955-521621730(K) (I)
1228.58861228.6286-3328380(K) (T)
1228.58861228.6901-833143241(R) (D)
1465.71531465.8491-913313441(K) (I)
1475.72721475.72382.36006110(K) (L)
1480.74851480.7912-293483591(K) (D)
1487.67781487.7018-1639510(R) (L)
1675.71941675.7312-7.12232380(K) (L)
2774.38922774.4035-5.13603861(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (128/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/47 matches (17%).
Acc. #: Species: MOUSE Name: RAB GDP dissociation inhibitor beta-2 (RAB GDI beta-2) (GDI-3)
Index: MW: 50538 Da pI: 5.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
999.5671999.4933741661731(K) (K)
1109.51231109.6029-8290980(K) (Y)
1179.56441179.6737-932092181(R) (Y)
1251.58691251.6989-893803900(K) (D)
1344.63871344.6840-341431530(K) (D)
1450.66901450.5870564244350(R) (R)
2211.13452211.2034-3180981(K) (Y)
2384.00712384.2423-991161371(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (93/445AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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