MS-Fit Search Results



.

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B50
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 3933 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/95(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.822e+00819 (20)32.020.0-7.7353.4 73681/5.9HUMANStress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)
21.172e+00822 (23)44.023.2-22.056.9 53675/5.5HUMANKeratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
35.428e+00717 (17)29.017.9-7.7956.4 73529/5.9MOUSEStress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin)
41.117e+00614 (14)25.014.7-20.566.1 70872/5.4MOUSEHeat shock cognate 71 kDa protein
51.116e+00614 (14)25.014.7-20.566.1 70899/5.4HUMANHeat shock cognate 71 kDa protein
69.870e+00515 (15)17.015.8-2.93113 84050/6.3MOUSEDystrobrevin alpha (Alpha-dystrobrevin)
73.922e+00514 (14)20.014.7-17.182.9 65871/8.4HUMANKeratin, type II cytoskeletal 2 oral (Cytokeratin 2P) (K2P) (CK 2P)
82.793e+00514 (14)15.014.7-10.9112 83935/6.4HUMANDystrobrevin alpha (Dystrobrevin-alpha)
91.763e+00520 (21)37.021.1-15.488.2 54450/5.5MOUSEKeratin, type II cytoskeletal 8 (Cytokeratin 8) (Cytokeratin endo A)
101.671e+00515 (15)20.015.80.50894.8 82268/9.0HUMANZinc finger protein 224
57265.x........x......x.....x..............x.............x....x..x.xx....x....xx.x.......xx..xx....x
28336.............x...xx....x..x.xx.....x.x.....x.....x...x..x...xx..x...x.x......x..x..x......x....
32573.x........x......x.....x..............x.............x....x..x.......x....xx.x.......xx..xx....x
101395.x......x....................................xx..x...xx....x..............x.....xx.....x...x.x.
99823.x......x....................................xx..x...xx....x..............x.....xx.....x...x.x.
21748x................x..x...x............................x...x.......x....x..x..xxxx....x..x.......
31846..x......x....x...x....x.....x......xx..xx.....xx.................x.....................x......
3161x................x..x...x............................x...x.......x.......x..xxx.....x..x..x....
51565...........x.x....x..x......xx.....x...x...x..x..x.x..x...x..x..x.....x......x..x..x...........
68039.................x.x.x..x............x..x.x...x.x.x.....x........x......x..............xx......



Detailed Results

1. 19/95 matches (20%).
Acc. #: Species: HUMAN Name: Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)
Index: MW: 73681 Da pI: 5.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
958.4547958.4958-4377850(K) (T)
994.4357994.4515-161Met-ox 3693770(K)AMQDAEVSK(S)
1028.49081028.5675-751Met-ox 3413481(K)HLNMKLTR(A)
1045.50781045.5392-301Met-ox 6476541(K)LFEMAYKK(M)
1148.52911148.5548-226256341(R) (Q)
1242.65741242.6806-192072180(K) (V)
1290.66011290.6806-163954050(K) (A)
1341.65591341.6188281471590(R) (L)
1349.65921349.65890.261281381(R) (N)
1361.72281361.7429-153493600(R) (R)
1405.71331405.6633365565671(K) (Y)
1446.73451446.7626-193783910(K) (M)
1450.71081450.7178-4.886990(R) (L)
1462.75161462.7576-4.11Met-ox 3783910(K)SDIGEVILVGGMTR(M)
1592.94361592.9528-5.84995130(K) (G)
1645.88391645.88012.32192340(R) (S)
1694.86331694.85027.71882020(K) (Q)
1706.85771706.8383111Met-ox 2933071(R)ETGVDLTKDNMALQR(V)
2056.03932055.9623372662840(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (223/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 22/95 matches (23%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.49521016.49470.45pyroGlu 1Met-ox 3653721(K)QDMARQLR(E)
1028.49081028.5264-35pyroGlu 1501580(R)QLETLGQEK(L)
1030.52341030.5686-441231300(K) (T)
1045.50781045.5530-431501580(R) (L)
1062.48341062.5108-26pyroGlu 2262330(R)QLYEEEIR(E)
1079.50281079.5122-8.72652730(K) (S)
1079.50281079.5373-322262330(R) (E)
1082.51331082.5999-801021101(K) (F)
1129.58541129.6217-323423521(R) (L)
1129.58541129.6217-323533620(K) (A)
1137.54131137.5792-332862950(K) (H)
1169.54901169.5512-1.92762850(R) (Y)
1169.54901169.5512-1.91Met-ox 3733810(R)EYQELMNVK(L)
1228.60671228.5520451Met-ox 1982071(K)KDVDEAYMNK(V)
1251.59041251.6446-4312231(K) (S)
1277.68851277.7105-173823920(K) (K)
1341.65591341.7490-693173281(R) (A)
1344.65641344.6759-153293410(R) (G)
1368.64301368.6721-211Met-ox 1871970(R)TEMENEFVLIK(K)
1405.71331405.8055-663823931(K) (L)
1419.72561419.7484-162142250(R) (Q)
1475.74131475.6987294024140(R) (T)
1480.76731480.7721-3.31871981(R) (D)
1566.77711566.7950-111Met-ox 3703811(R)QLREYQELMNVK(L)
1762.84801762.83606.824401(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 44% (213/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 17/95 matches (17%).
Acc. #: Species: MOUSE Name: Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin)
Index: MW: 73529 Da pI: 5.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
958.4547958.4958-4377850(K) (T)
994.4357994.4515-161Met-ox 3693770(K)AMQDAEVSK(S)
1028.49081028.5675-751Met-ox 3413481(K)HLNMKLTR(A)
1045.50781045.5392-301Met-ox 6476541(K)LFEMAYKK(M)
1148.52911148.5548-226256341(R) (Q)
1242.65741242.6806-192072180(K) (V)
1290.66011290.6806-163954050(K) (A)
1341.65591341.6188281471590(R) (L)
1405.71331405.6633365565671(K) (Y)
1446.73451446.7626-193783910(K) (M)
1450.71081450.7178-4.886990(R) (L)
1462.75161462.7576-4.11Met-ox 3783910(K)SDIGEVILVGGMTR(M)
1592.94361592.9528-5.84995130(K) (G)
1645.88391645.88012.32192340(R) (S)
1694.86331694.85027.71882020(K) (Q)
1706.85771706.8383111Met-ox 2933071(R)ETGVDLTKDNMALQR(V)
2056.03932055.9623372662840(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (200/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/95 matches (14%).
Acc. #: Species: MOUSE Name: Heat shock cognate 71 kDa protein
Index: MW: 70872 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
958.4547958.5396-896026090(K) (L)
989.4614989.4904-295625691(K) (I)
989.4614989.5267-665105171(R) (M)
1197.64041197.6632-194594690(K) (G)
1199.64391199.6748-261601710(K) (I)
1228.60671228.6286-1826360(K) (T)
1251.59041251.6196-231Met-ox 2372460(R)MVNHFIAEFK(R)
1253.60681253.6166-7.93023110(R) (G)
1319.64681319.5942401Met-ox 5405500(K)NSLESYAFNMK(A)
1450.71081450.8058-653463571(K) (E)
1480.76731480.75498.43003111(R) (G)
1487.69571487.7018-4.137490(R) (L)
1691.74171691.72619.22212360(K) (M)
1837.97201838.0136-233263421(K) (I)
1982.00841981.99835.11381550(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (167/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/95 matches (14%).
Acc. #: Species: HUMAN Name: Heat shock cognate 71 kDa protein
Index: MW: 70899 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
958.4547958.5396-896026090(K) (L)
989.4614989.4904-295625691(K) (I)
989.4614989.5267-665105171(R) (M)
1197.64041197.6632-194594690(K) (G)
1199.64391199.6748-261601710(K) (I)
1228.60671228.6286-1826360(K) (T)
1251.59041251.6196-231Met-ox 2372460(R)MVNHFIAEFK(R)
1253.60681253.6166-7.93023110(R) (G)
1319.64681319.5942401Met-ox 5405500(K)NSLESYAFNMK(A)
1450.71081450.8058-653463571(K) (E)
1480.76731480.75498.43003111(R) (G)
1487.69571487.7018-4.137490(R) (L)
1691.74171691.72619.22212360(K) (M)
1837.97201838.0136-233263421(K) (I)
1982.00841981.99835.11381550(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (167/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 15/95 matches (15%).
Acc. #: Species: MOUSE Name: Dystrobrevin alpha (Alpha-dystrobrevin)
Index: MW: 84050 Da pI: 6.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
951.4084951.4570-511Met-ox 181(-)MIEDSGKR(G)
1028.49081028.5740-814824890(R) (L)
1035.47351035.5331-581341430(K) (I)
1051.61971051.5280871Met-ox 1341430(K)MALATLCGGK(I)
1251.59041251.6810-724274361(R) (Y)
1290.66011290.6802-165385480(R) (L)
1378.69911378.68549.94554670(R) (Q)
1419.72561419.6902253673781(K) (A)
1446.73451446.7813-325375481(R) (L)
1462.75161462.7762-171Met-ox 5375481(R)RELMVQLEGLMK(L)
1475.74131475.6562582742870(R) (E)
1478.75711478.7711-9.42Met-ox 5375481(R)RELMVQLEGLMK(L)
1479.78031479.7080494925040(R) (A)
1592.94361592.78559917291(R) (I)
1691.74171691.8023-365235361(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (127/746AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 14/95 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 oral (Cytokeratin 2P) (K2P) (CK 2P)
Index: MW: 65871 Da pI: 8.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
965.4308965.4661-371Met-ox 171(-)MNRQVCK(K)
991.4592991.4883-293994060(K) (M)
1019.46761019.4832-154884950(K) (M)
1030.52341030.5533-292442520(K) (G)
1045.50781045.5642-543803890(K) (H)
1082.51331082.5999-801942021(K) (F)
1134.52411134.6119-7766770(K) (A)
1137.54131137.5540-113593670(R) (S)
1157.62031157.6530-284474560(K) (A)
1163.56911163.5730-3.41Met-ox 4364461(R)GEMALKDANAK(L)
1211.64791211.5618712612700(K) (K)
1227.60781227.5567421Met-ox 2612700(K)SMQDLVEDFK(K)
1380.70091380.7011-0.163083190(K) (T)
1694.86331694.8423121Met-ox 2933071(K)DVDAAFMNKVELQAK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (130/638AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 14/95 matches (14%).
Acc. #: Species: HUMAN Name: Dystrobrevin alpha (Dystrobrevin-alpha)
Index: MW: 83935 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
951.4084951.4570-511Met-ox 181(-)MIEDSGKR(G)
1028.49081028.5740-814854920(R) (L)
1035.47351035.5331-581341430(K) (I)
1051.61971051.5280871Met-ox 1341430(K)MALATLCGGK(I)
1251.59041251.6810-724304391(R) (Y)
1290.66011290.6802-165415510(R) (L)
1378.69911378.68549.94584700(R) (Q)
1446.73451446.7813-325405511(R) (L)
1462.75161462.7762-171Met-ox 5405511(R)RELMVQLEGLMK(L)
1475.74131475.6562582742870(R) (E)
1478.75711478.7711-9.42Met-ox 5405511(R)RELMVQLEGLMK(L)
1592.94361592.78559917291(R) (I)
1691.74171691.8023-365265391(K) (R)
1762.84801762.9088-344935071(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (117/743AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 20/95 matches (21%).
Acc. #: Species: MOUSE Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (Cytokeratin endo A)
Index: MW: 54450 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
998.4192998.5159-97pyroGlu 1561640(R)QLEALGQEK(L)
1016.49521016.49470.45pyroGlu 1Met-ox 3703771(K)QDMARQLR(E)
1030.52341030.5686-441291360(K) (T)
1036.50051036.5063-5.7pyroGlu 2322390(R)QIHEEEIR(E)
1079.50281079.5122-8.72712790(R) (S)
1082.51331082.5999-801081161(K) (F)
1129.58541129.5312481Met-ox 9181(K)SYKMSTSGPR(A)
1151.61981151.5948222923010(K) (H)
1169.54901169.5512-1.91Met-ox 3783860(R)EYQELMNVK(L)
1199.64391199.5618681Met-ox 2822910(R)AEAETMYQIK(Y)
1228.60671228.5520451Met-ox 2042131(K)KDVDEAYMNK(V)
1239.63031239.6949-52pyroGlu 1561661(R)QLEALGQEKLK(L)
1253.60681253.6854-6384940(K) (T)
1253.60681253.7105-833583690(K) (Q)
1307.67471307.7211-363873970(K) (K)
1344.65641344.6759-153343460(R) (G)
1368.64301368.6721-211Met-ox 1932030(R)TEMENEFVLIK(K)
1419.72561419.7484-162202310(R) (Q)
1475.74131475.6987294074190(R) (T)
1480.76731480.7721-3.31932041(R) (D)
1566.77711566.7950-111Met-ox 3753861(R)QLREYQELMNVK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (185/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 15/95 matches (15%).
Acc. #: Species: HUMAN Name: Zinc finger protein 224
Index: MW: 82268 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1028.49081028.5529-603934010(R) (C)
1032.52621032.4937315255320(K) (V)
1032.52621032.5114144214290(R) (C)
1036.50051036.5427-416326400(K) (V)
1051.61971051.539776pyroGlu 3273351(R)QRSALNSHR(M)
1137.54131137.6203-694684761(R) (L)
1157.62031157.5777371841920(K) (I)
1165.52771165.5498-191972051(R) (C)
1199.64391199.5553744554631(K) (S)
1199.64391199.6285134404481(R) (V)
1227.60781227.5614382342431(K) (R)
1234.61651234.6479-251932021(R) (C)
1277.68851277.68621.765741(K) (E)
1378.69911378.7344-2643540(R) (D)
1439.78691439.7218453063170(R) (C)
1691.74171691.8804-821841961(K) (V)
1694.86331694.86141.14414541(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (143/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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