MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B58
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 200000 Da) selects 113421 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 13705 entries.
Pre searches select 13705 entries.

Data Set 1 Results

MS-Fit search selects 6533 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/116(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.133e+01026 (22)49.022.442.862.1 50977/5.4HUMANHeterogeneous nuclear ribonucleoprotein K (hnRNP K) (DC-stretch binding protein) (CSBP) (Transformation upregulated nuclear protein) (TUNP)
29.930e+00925 (21)46.021.642.563.3 50994/5.3MOUSEHeterogeneous nuclear ribonucleoprotein K (hnRNP K) (65 kDa phosphoprotein)
32.701e+00730 (25)24.025.921.8104 151831/9.7MOUSERetrovirus-related POL polyprotein [Contains: Reverse transcriptase ; Endonuclease]
42.308e+00739 (33)28.033.61.3796.1 180068/6.4HUMANHypothetical protein KIAA0373
53.235e+00624 (20)14.020.75.61101 198671/5.5MOUSEBreast cancer type 1 susceptibility protein homolog
9697..xx..xx....x.......xx...xx.....xx.x.....x....x.........x..x..........x...x..........x.xx..xx........x...x.........x
75381..xx..xx....x.......xx...xx.....xx.x.....x....x.........x.............x...x..........x.xx..xx........x...x.........x
2454......x..x.x.xx.....x..xxxx..x.xx......x...xx.x...........x......x.x................x.......xxx...xx.xx....x......x.
83572.x..x.x...x..x.xxxxxx.xx.......x........xx.x...xx...xx....x.x..x...xxx.....xx.......xx..xx.x.x.x.....x...x...x......
101958..xxx..x..x...............x..xx.xx........xxxx.......x.............xxx......x..............x..x.........x.....x...x.


Detailed Results

1. 26/116 matches (22%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) (DC-stretch binding protein) (CSBP) (Transformation upregulated nuclear protein) (TUNP)
Index: MW: 50977 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4911997.4413503173250(R) (R)
998.4435998.3889552792860(R) (R)
1013.46671013.4362301Met-ox 3173250(R)GGDLMAYDR(R)
1014.44001014.3838551Met-ox 2792860(R)DYDDMSPR(R)
1053.68161053.6421381922010(R) (V)
1106.56231106.51524338460(R) (I)
1112.51381112.4682411401480(K) (L)
1122.55061122.5101361Met-ox 38460(R)NTDEMVELR(I)
1126.53921126.5904-451Met-ox 2692781(R)GGRPMPPSRR(D)
1153.57661153.5424303173261(R) (G)
1169.58271169.5373391Met-ox 3173261(R)GGDLMAYDRR(G)
1194.75331194.6999453063160(R) (G)
1259.64331259.5756544234330(K) (I)
1340.85911340.8041412082190(K) (G)
1505.88871505.74949235462(K) (I)
1519.01501518.9372511491630(R) (G)
1577.77031577.6841551801910(K) (V)
1595.79391595.7012581Met-ox 22340(K)RPAEDMEEEQAFK(R)
1735.90591735.80745722351(K) (S)
1751.90081751.8023561Met-ox 22351(K)RPAEDMEEEQAFKR(S)
1762.00441761.9083542482632(R) (M)
1778.90751779.0142-603003162(R) (G)
1780.90861780.79906270860(R) (I)
1843.16241843.079245871031(R) (I)
1917.16641917.0333693783960(R) (D)
2589.55142589.3888634344560(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (231/463AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 25/116 matches (21%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) (65 kDa phosphoprotein)
Index: MW: 50994 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4911997.4413503173250(R) (R)
998.4435998.3889552792860(R) (R)
1013.46671013.4362301Met-ox 3173250(R)GGDLMAYDR(R)
1014.44001014.3838551Met-ox 2792860(R)DYDDMSPR(R)
1053.68161053.6421381922010(R) (V)
1106.56231106.51524338460(R) (I)
1112.51381112.4682411401480(K) (L)
1122.55061122.5101361Met-ox 38460(R)NTDEMVELR(I)
1126.53921126.5904-451Met-ox 2692781(R)GGRPMPPSRR(D)
1153.57661153.5424303173261(R) (G)
1169.58271169.5373391Met-ox 3173261(R)GGDLMAYDRR(G)
1194.75331194.6999453063160(R) (G)
1259.64331259.5756544234330(K) (I)
1340.85911340.8041412082190(K) (G)
1505.88871505.74949235462(K) (I)
1577.77031577.6841551801910(K) (V)
1595.79391595.7012581Met-ox 22340(K)RPAEDMEEEQAFK(R)
1735.90591735.80745722351(K) (S)
1751.90081751.8023561Met-ox 22351(K)RPAEDMEEEQAFKR(S)
1762.00441761.9083542482632(R) (M)
1778.90751779.0142-603003162(R) (G)
1780.90861780.79906270860(R) (I)
1843.16241843.079245871031(R) (I)
1917.16641917.0333693783960(R) (D)
2589.55142589.3888634344560(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (216/464AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 30/116 matches (25%).
Acc. #: Species: MOUSE Name: Retrovirus-related POL polyprotein [Contains: Reverse transcriptase ; Endonuclease]
Index: MW: 151831 Da pI: 9.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1013.46671013.5606-936346410(K) (V)
1029.58681029.5555301Met-ox 6346410(K)IQHPFMIK(V)
1029.58681029.6169-293613682(R) (L)
1045.59981045.5278693713790(R) (R)
1056.56941056.4638100110511120(K) (M)
1056.56941056.522744105710640(K) (I)
1061.49671061.5777-7618261(K) (I)
1106.56231106.5040534494570(K) (F)
1115.51901115.6173-887727802(K) (E)
1117.53731117.5464-8.18278350(K) (I)
1122.55061122.4989461Met-ox 4494570(K)LENLDEMDK(F)
1126.53921126.5897-45106510731(R) (G)
1136.55441136.5669-11102410331(R) (I)
1152.60911152.5618411Met-ox 102410331(R)TAMACAVRSR(I)
1153.57661153.519949pyroGlu 1Met-ox 2002080(K)QMDLTDIYR(T)
1222.70951222.6214721Met-ox 114511552(R)MAKIKNSGDSR(C)
1292.72041292.7003164394482(R) (L)
1316.74321316.6176959129201(K) (Q)
1340.85911340.780359113411441(K) (M)
1516.81901516.7834237597711(K) (N)
1552.86261552.815730104210542(K) (T)
1562.82311562.8576-221Met-ox 1871992(K)LNRDTVKLTEVMK(Q)
1731.07531730.9805556586731(K) (L)
1780.90861780.89448.08588720(K) (F)
1791.03131790.8787852002131(K) (T)
1794.93771794.9642-157437580(R) (I)
1818.02551817.9948176426571(K) (A)
1834.07931833.9897491Met-ox 6426571(K)VLERSGIQGPYLNMIK(A)
1843.16241843.032970971131(K) (V)
1850.06541850.0904-1498710012(K) (L)
1954.21861954.0299973813952(R) (I)
2473.26542473.2913-10126812881(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (321/1300AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 39/116 matches (33%).
Acc. #: Species: HUMAN Name: Hypothetical protein KIAA0373
Index: MW: 180068 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
994.4685994.5209-537527590(K) (E)
1007.49981007.5526-52pyroGlu 146014671(K)QHLKEEIK(K)
1013.46671013.5056-383063130(K) (L)
1036.54121036.5535-121Met-ox 3583651(K)LKIMQEMK(N)
1056.56941056.56890.40pyroGlu 135813661(K)QLVKEATER(E)
1068.54891068.5689-196696780(K) (H)
1075.61691075.6264-8.8132513331(K) (M)
1079.57381079.5407315325401(R) (L)
1086.49551086.5795-77128812960(K) (M)
1098.49781098.5328-32115811651(K) (E)
1098.49781098.5795-74pyroGlu 9319390(K)QSLIEELQR(K)
1106.56231106.55169.71Met-ox 3493570(K)TMIQLQNDK(L)
1114.51351114.5277-131Met-ox 115811651(K)EMCEFLKK(E)
1115.51901115.6061-789319390(K) (K)
1152.60911152.6741-56146014682(K) (L)
1241.64091241.7330-74126212712(K) (E)
1259.64331259.6596-13125812681(K) (L)
1292.72041292.7551-27116811782(K) (G)
1344.77091344.748717127612872(K) (N)
1344.77091344.7739-2.2144314532(K) (M)
1410.71821410.70519.31Met-ox 2913011(K)MEAYNLRLEQK(L)
1453.80821453.8379-20pyroGlu 9319422(K)QSLIEELQRKVK(K)
1472.74401472.7895-3138491(R) (T)
1472.74401472.8398-65118611981(K) (K)
1516.81901516.8223-2.1112411361(K) (F)
1522.79001522.7609191Met-ox 1191301(K)ITMLEMKELNER(Q)
1522.79001522.8229-22141414261(K) (D)
1536.85271536.700499pyroGlu 1491601(K)QMEERNFELETK(F)
1562.82311562.7848257607721(K) (E)
1563.75821563.8144-366366471(K) (L)
1568.88121568.7266992452570(R) (S)
1609.82331609.81862.97307421(K) (V)
1620.92801620.823365137013821(K) (I)
1731.07531730.9587675225352(K) (S)
1735.90591735.8536301Met-ox 1741881(K)VEQMLRDELADSVSK(A)
1762.00441761.917649pyroGlu 3333481(R)QFSGALPLAQQEKFSK(T)
1767.03441767.0129125695842(R) (K)
1778.90751778.9176-5.77457591(K) (E)
1778.90751778.9441-213333481(R) (T)
1791.03131790.9257591191322(K) (A)
1791.03131790.957441144514592(K) (Q)
1800.93671800.88023150640(R) (E)
1843.16241843.0037865455602(K) (V)
1917.16641917.01817765802(K) (L)
1980.25251980.124265pyroGlu 9139302(R)QIKRLTSGLQGKPLTDNK(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (443/1539AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 24/116 matches (20%).
Acc. #: Species: MOUSE Name: Breast cancer type 1 susceptibility protein homolog
Index: MW: 198671 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4911997.5067-165615690(K) (K)
998.4435998.5423-994574641(K) (K)
1007.49981007.44435539450(K) (F)
1014.44001014.4679-278718790(K) (E)
1036.54121036.5791-37169717042(R) (G)
1126.53921126.6373-874574652(K) (G)
1136.55441136.5812-246736811(K) (K)
1136.55441136.5812-246746821(K) (R)
1140.52691140.499524163416420(K) (T)
1153.57661153.5966-17pyroGlu 3073170(K)QPGIAVSQQSR(W)
1169.58271169.6353-456136220(R) (N)
1169.58271169.6503-58116211712(K) (L)
1262.67111262.66663.51Met-ox 159216030(R)DISMVVSGLTPK(E)
1292.72041292.6824296746832(K) (R)
1316.74321316.6810477427541(R) (S)
1319.71741319.590296pyroGlu 7307411(R)QMSDSAKELGDR(V)
1472.74401472.720816121312251(R) (G)
1562.82311562.7233643233361(K) (V)
1563.75821563.686246112311360(K) (S)
1568.88121568.7743688718841(K) (V)
1620.92801620.8862268578701(R) (D)
1778.90751778.8570284264400(K) (S)
1794.93771794.8519481Met-ox 4264400(K)TDLVTPDPHHTLMCK(S)
1794.93771794.917211136313770(K) (G)
1897.92971898.0486-63161716330(R) (T)
2069.21612069.066772150915271(R) (D)
2473.26542473.2363124444642(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (269/1812AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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