MS-Fit Search Results


.

Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B60
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 1751 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/112(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
18.771e+00921 (18)42.018.8-12.619.1 50977/5.4HUMANHeterogeneous nuclear ribonucleoprotein K (hnRNP K) (DC-stretch binding protein) (CSBP) (Transformation upregulated nuclear protein) (TUNP)
21.577e+00920 (17)39.017.9-12.719.6 50994/5.3MOUSEHeterogeneous nuclear ribonucleoprotein K (hnRNP K) (65 kDa phosphoprotein)
36.191e+00410 (8)16.08.90.34360.2 85660/6.0HUMANGlucocorticoid receptor (GR)
45.425e+00410 (8)14.08.9-0.074859.6 86876/9.7HUMANZinc finger protein 337
54.208e+0048 (7)17.07.10.42053.3 66472/6.7MOUSERING finger protein 12 (LIM domain interacting RING finger protein) (RING finger LIM domain-binding protein) (R-LIM)
9697..xx....x........xxx....x.x..x...x.....x...x..............x..........x.x............x.x....x........x..x.......x
75381..xx....x........xxx....x.x..x...x.....x...x.........................x.x............x.x....x........x..x.......x
8630.......x..............x........x...x....x............x.x.....x............x...............................x.....
17758......x........x..................x...........x.xx.................................x.........x..x....x..........
82136.x............................x..................x...x.....x...........x........x...............x...............


Detailed Results

1. 21/112 matches (18%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) (DC-stretch binding protein) (CSBP) (Transformation upregulated nuclear protein) (TUNP)
Index: MW: 50977 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1013.42831013.4362-7.81Met-ox 3173250(R)GGDLMAYDR(R)
1014.37731014.3838-6.51Met-ox 2792860(R)DYDDMSPR(R)
1053.62281053.6421-181922010(R) (V)
1106.49791106.5152-1638460(R) (I)
1112.45341112.4682-131401480(K) (L)
1122.48301122.5101-241Met-ox 38460(R)NTDEMVELR(I)
1153.50631153.5424-313173261(R) (G)
1169.51541169.5373-191Met-ox 3173261(R)GGDLMAYDRR(G)
1194.68981194.6999-8.53063160(R) (G)
1259.57351259.5756-1.64234330(K) (I)
1340.78221340.8041-162082190(K) (G)
1365.61241365.6433-231Met-ox 36461(R)SRNTDEMVELR(I)
1518.92301518.9372-9.31491630(R) (G)
1577.67491577.6841-5.81801910(K) (V)
1595.68351595.7012-111Met-ox 22340(K)RPAEDMEEEQAFK(R)
1735.78111735.8074-1522351(K) (S)
1751.78261751.8023-111Met-ox 22351(K)RPAEDMEEEQAFKR(S)
1780.79551780.7990-1.970860(R) (I)
1843.02421843.0792-30871031(R) (I)
1917.03281917.0333-0.293783960(R) (D)
2589.40312589.38885.54344560(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (199/463AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 20/112 matches (17%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) (65 kDa phosphoprotein)
Index: MW: 50994 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1013.42831013.4362-7.81Met-ox 3173250(R)GGDLMAYDR(R)
1014.37731014.3838-6.51Met-ox 2792860(R)DYDDMSPR(R)
1053.62281053.6421-181922010(R) (V)
1106.49791106.5152-1638460(R) (I)
1112.45341112.4682-131401480(K) (L)
1122.48301122.5101-241Met-ox 38460(R)NTDEMVELR(I)
1153.50631153.5424-313173261(R) (G)
1169.51541169.5373-191Met-ox 3173261(R)GGDLMAYDRR(G)
1194.68981194.6999-8.53063160(R) (G)
1259.57351259.5756-1.64234330(K) (I)
1340.78221340.8041-162082190(K) (G)
1365.61241365.6433-231Met-ox 36461(R)SRNTDEMVELR(I)
1577.67491577.6841-5.81801910(K) (V)
1595.68351595.7012-111Met-ox 22340(K)RPAEDMEEEQAFK(R)
1735.78111735.8074-1522351(K) (S)
1751.78261751.8023-111Met-ox 22351(K)RPAEDMEEEQAFKR(S)
1780.79551780.7990-1.970860(R) (I)
1843.02421843.0792-30871031(R) (I)
1917.03281917.0333-0.293783960(R) (D)
2589.40312589.38885.54344560(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (184/464AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/112 matches (8%).
Acc. #: Species: HUMAN Name: Glucocorticoid receptor (GR)
Index: MW: 85660 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.54251045.5947-505775851(K) (N)
1136.51941136.5588-356826900(K) (M)
1206.67001206.6339305595690(R) (Q)
1292.64891292.6091311Met-ox 4814910(K)CLQAGMNLEAR(K)
1344.68421344.66221624341(R) (G)
1488.75511488.7018364484600(R) (N)
1495.71751495.71412.31591710(K) (G)
1534.76791534.7827-9.62812930(K) (Q)
1620.82651620.80811141570(K) (Q)
2211.09822211.1621-291211401(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (127/777AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/112 matches (8%).
Acc. #: Species: HUMAN Name: Zinc finger protein 337
Index: MW: 86876 Da pI: 9.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1041.51571041.51510.552682761(K) (S)
1098.45141098.5002-443243321(K) (S)
1273.60631273.6363-243133230(K) (E)
1418.71361418.7466-231261380(K) (H)
1434.72441434.7415-121Met-ox 1261380(K)STKPMAFSSPPLR(H)
1442.76981442.7432183283391(R) (R)
1724.94861724.8655483944071(R) (D)
1792.89761792.88308.22192340(R) (S)
1817.87651817.9042-156786911(R) (R)
1879.88651879.8067422852990(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (108/751AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/112 matches (7%).
Acc. #: Species: MOUSE Name: RING finger protein 12 (LIM domain interacting RING finger protein) (RING finger LIM domain-binding protein) (R-LIM)
Index: MW: 66472 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1007.44781007.4546-6.81271350(R) (F)
1197.69411197.6592292662760(R) (G)
1442.76981442.7313272272390(R) (R)
1488.75511488.7844-205235351(R) (S)
1522.68051522.7284-311611731(R) (Q)
1595.68351595.7302-293403530(R) (G)
1707.77391707.77201.11741890(R) (A)
1817.87651817.8153342412570(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (106/600AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.