MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B69
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3938 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/110(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.866e+00813 (11)36.011.892.532.5 38890/4.9HUMANReticulocalbin 1 precursor
27.448e+00712 (10)35.010.993.531.8 49974/4.7HUMANPlacental ribonuclease inhibitor (Ribonuclease/angiogenin inhibitor) (RAI) (RI)
36.152e+00719 (17)26.017.392.098.3 80897/9.1MOUSEPlakophilin 1
41.478e+00718 (16)32.016.491.695.9 82806/9.1HUMANPoly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
55.885e+00616 (14)27.014.572.089.6 69399/5.9HUMANEzrin (p81) (Cytovillin) (Villin 2)
63.299e+0069 (8)29.08.291.826.9 38113/4.7MOUSEReticulocalbin 1 precursor
72.686e+00612 (10)18.010.910036.3 78247/5.0HUMANSecretogranin I precursor (SGI) (Chromogranin B) (CgB) [Contains: GAWK peptide; CCB peptide]
82.134e+00613 (11)19.011.826.8160 92876/6.0HUMANMutS protein homolog 5
91.610e+00611 (10)24.010.010149.2 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
101.578e+0068 (7)26.07.391.672.5 44156/5.9HUMANZinc-finger protein ubi-d4 (Requiem) (Apoptosis response zinc finger protein)
60425..x.x...x.............x.........x.......................x.......x..................x......xx....x...........xx
60756......x..................x....x......x.....x...x.....xx.....x..................x.................x......x.....
105569............x..x.........x.x.x..x.x....x......xx...x..x........x...............x..........xx......x....x..x...
108537........x......x......x.xx.x.x........x......x...x............x..........x..........x.x...x.......x....x....x.
6380x.xx..xx...............x....x..x................x..........x............x.........x..x.........x..x..........x
20661..x.....x..................................x.....x.................................x......xx................xx
57846.x.......x.x...................x....................x.....x......................x.....x.......x....x.......xx
72737.x.....x.....x..........x......x.........x.x....x.................x....x..................x......x...x........
45313......................xx...x.....x......x.....x..................x...x.....x............x..............x......
94406.............x.................................................x.....x................x..x............x.xx....


Detailed Results

1. 13/110 matches (11%).
Acc. #: Species: HUMAN Name: Reticulocalbin 1 precursor
Index: MW: 38890 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
954.5496954.4573971351410(K) (Q)
969.5579969.44301192502560(R) (D)
969.5579969.5046551151220(R) (V)
990.5908990.48961022872940(R) (N)
1125.70871125.6057921141221(K) (V)
1280.74551280.601011371810(K) (E)
1565.89451565.74479671831(K) (L)
1652.93451652.7655102911050(R) (T)
1950.09631949.9258872712860(R) (H)
2021.10352020.9115951892040(R) (E)
2037.10192036.9064961Met-ox 1892040(R)EEFTAFLHPEEFEHMK(E)
2136.22932136.046186871051(K) (T)
2785.47342785.2283881421650(K) (K)
2913.52062913.3232681421661(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (120/331AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/110 matches (10%).
Acc. #: Species: HUMAN Name: Placental ribonuclease inhibitor (Ribonuclease/angiogenin inhibitor) (RAI) (RI)
Index: MW: 49974 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
976.5783976.5137662182260(R) (A)
1163.65081163.53679835440(R) (C)
1209.81991209.695510354640(R) (S)
1346.88151346.74321033423520(R) (L)
1425.78901425.61021252062170(K) (D)
1491.89431491.7670852602710(R) (G)
1527.94481527.7953983603730(R) (V)
1530.90751530.74001092883020(K) (L)
1630.94671630.8037881741880(K) (V)
1867.17581867.02648020340(R) (L)
2148.18032148.013178AcetN 1190(-)SLDIQSLDIQCEELSDAR(W)
2395.41152395.2001881471670(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (164/461AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 19/110 matches (17%).
Acc. #: Species: MOUSE Name: Plakophilin 1
Index: MW: 80897 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1011.54551011.5951-495745821(K) (L)
1045.68431045.56421153193271(R) (R)
1163.65081163.6135326876960(R) (E)
1179.72181179.6084961Met-ox 6876960(R)LLLSDMWASK(E)
1187.77531187.63841155835930(R) (S)
1280.74551280.59791152Met-ox 6476570(R)NLMTSQPQMAK(Q)
1307.80731307.6782992822920(K) (L)
1407.82261407.64731252Met-ox 7107220(R)NMMGNIAGANNLR(N)
1475.90931475.7350118pyroGlu 1Met-ox 39501(R)QRVQEQVMMTVK(R)
1491.89431491.7300110pyroGlu 2Met-ox 39501(R)QRVQEQVMMTVK(R)
1513.92931513.7361128pyroGlu 2112230(K)QDPVYVPPISCNK(D)
1530.90751530.7626952112230(K) (D)
1650.98281650.76201341851981(R) (K)
1867.17581866.90601453974120(R) (N)
2021.10352020.9373826586730(K) (N)
2037.10192036.9322831Met-ox 6586730(K)QYFSNSMLNNVFNLCR(N)
2210.24032209.92041455015180(K) (G)
2384.13652384.0586331Met-ox 1301501(R)HYPRGSCATPGAGSDICFMQK(I)
2705.32292705.245229pyroGlu 1Met-ox 6586801(K)QYFSNSMLNNVFNLCRNTASSPK(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (191/728AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 18/110 matches (16%).
Acc. #: Species: HUMAN Name: Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
Index: MW: 82806 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
990.5908990.504987961030(K) (L)
1045.68431045.54641324694770(R) (E)
1125.70871125.55401376466560(R) (R)
1140.72791140.6417766646721(K) (D)
1163.65081163.51801145505600(K) (N)
1163.65081163.5778631962041(R) (V)
1179.72181179.553414373820(K) (N)
1187.77531187.6676911471570(R) (F)
1350.82381350.63241423140(K) (Q)
1450.79861450.64501065365490(R) (S)
1509.92771509.73311295235351(K) (D)
1646.01321645.8146121pyroGlu 5075221(K)QSLSDVNRSSGGLQSK(R)
1803.09861802.85021381221351(R) (L)
1962.13681961.9490961Met-ox 6847020(K)SVDAIGGESMPIPTIDTSR(K)
1975.18271975.0136863423591(K) (L)
2021.10352021.0667187067241(R) (V)
2210.24032210.197419831031(K) (L)
2384.13652384.2145-331Met-ox 6186390(R)IITPHNPAQGQPHLNGMSNITK(T)
2785.47342785.2587775245491(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (238/736AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 16/110 matches (14%).
Acc. #: Species: HUMAN Name: Ezrin (p81) (Cytovillin) (Villin 2)
Index: MW: 69399 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
951.5473951.43921143363421(R) (E)
951.5473951.43921143383441(K) (E)
954.5496954.5559-6.6180(-) (V)
965.5709965.5056682472541(R) (F)
976.5783976.54683228350(K) (T)
983.5565983.42901302Met-ox 3363421(R)EKEQMMR(E)
983.5565983.42901302Met-ox 3383441(K)EQMMREK(E)
1127.66991127.5995621571651(R) (D)
1182.72651182.59481112642730(K) (L)
1218.73571218.58671221631711(K) (I)
1493.88931493.69191325485590(R) (Q)
1604.97491604.8484791721841(R) (D)
1791.94431791.8708412802930(R) (R)
1948.09771947.9719652802941(R) (R)
1948.09771947.9719652792931(K) (R)
1964.10261963.9669691Met-ox 2802941(R)ILQLCMGNHELYMRR(R)
1964.10261963.9669691Met-ox 2792931(K)RILQLCMGNHELYMR(R)
2105.13082105.12831.22122301(K) (D)
2210.24032210.13984536531(K) (G)
2913.52062913.5290-2.9841071(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (160/586AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/110 matches (8%).
Acc. #: Species: MOUSE Name: Reticulocalbin 1 precursor
Index: MW: 38113 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
954.5496954.4573971291350(K) (Q)
990.5908990.48961022812880(R) (N)
1425.78901425.6763792442541(R) (D)
1509.92771509.75491141691821(R) (E)
1950.09631949.9258872652800(R) (H)
2021.10352020.9115951831980(R) (E)
2037.10192036.9064961Met-ox 1831980(R)EEFTAFLHPEEFEHMK(E)
2785.47342785.2283881361590(K) (K)
2913.52062913.3232681361601(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (97/325AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 12/110 matches (10%).
Acc. #: Species: HUMAN Name: Secretogranin I precursor (SGI) (Chromogranin B) (CgB) [Contains: GAWK peptide; CCB peptide]
Index: MW: 78247 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
952.5492952.4753784404470(R) (V)
991.5552991.45191044484550(R) (A)
1007.58221007.44681341Met-ox 4484550(R)VQENQMDK(A)
1218.73571218.59011204484571(R) (R)
1519.86791519.7240956416531(K) (K)
1598.92221598.74911085745860(K) (R)
1941.11711940.90371101Met-ox 4404551(R)FLGEGHHRVQENQMDK(A)
1979.14241978.9583932112281(K) (T)
2105.13082104.9246983974151(K) (G)
2294.20222294.0465681341530(R) (T)
2785.47342785.17531076176400(K) (D)
2913.52062913.2703866166401(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (126/677AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 13/110 matches (11%).
Acc. #: Species: HUMAN Name: MutS protein homolog 5
Index: MW: 92876 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
952.5492952.6308-863153221(K) (M)
983.5565983.538718AcetN 1101(-)ASLGANPRR(T)
1013.57241013.4805915415480(R) (Y)
1140.72791140.56581425635710(R) (T)
1218.73571218.6846422502600(K) (C)
1422.90351422.9048-0.937807921(R) (K)
1425.78901425.63201105725840(R) (G)
1493.88931493.67281457948050(K) (F)
1667.93701667.8427574854980(K) (D)
1765.92441766.0540-737737881(K) (D)
2021.10352021.1509-231651810(K) (A)
2148.18032148.17054.61942121(R) (K)
2344.12152344.3029-771271461(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (161/834AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 11/110 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1125.70871125.54281473673760(K) (Y)
1127.66991127.54071152892981(K) (V)
1179.72181179.60101023773860(K) (H)
1300.71811300.53021452582670(K) (N)
1421.85011421.7389782772881(K) (K)
1475.90931475.74941082122230(K) (T)
1475.90931475.7858842002110(R) (W)
1657.92211657.79347813290(R) (R)
1688.10991687.97078266821(R) (G)
1814.07631813.894510013301(R) (T)
1994.16001993.9772922242390(R) (R)
2384.13652383.9524775195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (158/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/110 matches (7%).
Acc. #: Species: HUMAN Name: Zinc-finger protein ubi-d4 (Requiem) (Apoptosis response zinc finger protein)
Index: MW: 44156 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1013.57241013.53084111180(K) (D)
1650.98281650.76111341381520(R) (A)
1688.10991687.88671321091240(K) (T)
1975.18271974.96041133013180(R) (T)
2007.10522006.9502772Met-ox 3013180(R)SGHPSCLQFTPVMMAAVK(T)
2369.19062369.0906422612811(K) (I)
2395.41152395.17251003013211(R) (W)
2501.41832501.18459339590(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (102/391AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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