MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B70
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 2495 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/87(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.968e+00717 (19)57.019.516.943.7 38890/4.9HUMANReticulocalbin 1 precursor
21.667e+00511 (12)40.012.616.842.7 38113/4.7MOUSEReticulocalbin 1 precursor
31.379e+00510 (11)30.011.526.232.8 41737/5.3HUMANActin, cytoplasmic 1 (Beta-actin)
41.377e+00510 (11)30.011.526.232.8 41793/5.3HUMANActin, cytoplasmic 2 (Gamma-actin)
51.082e+00412 (13)18.013.8-3.4356.7 81610/9.0HUMANZinc finger protein 175 (Zinc finger protein OTK18)
61151.x.x..............x.....x...x.x........x..x.x.x...............x......xx.x.........xx.x.
20912.x......................x.....x........x......................x..x...xx...........xx.x.
54297......x............x.....x............xxx................x.....x.x.........x...........
111751......x............x.....x............xxx................x.....x.x.........x...........
15361.x.......x............x...........x.x..xx.x..........................xxx..........x....



Detailed Results

1. 17/87 matches (19%).
Acc. #: Species: HUMAN Name: Reticulocalbin 1 precursor
Index: MW: 38890 Da pI: 4.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
954.4604954.45733.31351410(K) (Q)
969.4709969.4430292502560(R) (D)
969.4709969.5046-351151220(R) (V)
1125.62261125.6057151141221(K) (V)
1188.64491188.6224192612702(K) (H)
1217.63441217.6126181771880(K) (E)
1232.60111232.6275-212872961(R) (D)
1515.78211515.8158-222052170(K) (N)
1565.78371565.74472571831(K) (L)
1604.83491604.7920272872992(R) (L)
1652.80931652.765526911050(R) (T)
1949.98141949.9258282712860(R) (H)
2020.97332020.9115311892040(R) (E)
2036.95522036.9064241Met-ox 1892040(R)EEFTAFLHPEEFEHMK(E)
2136.10432136.046127871051(K) (T)
2785.30892785.2283291421650(K) (K)
2913.45912913.3232471421661(K) (M)
3359.71243359.59333538660(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 57% (189/331AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/87 matches (12%).
Acc. #: Species: MOUSE Name: Reticulocalbin 1 precursor
Index: MW: 38113 Da pI: 4.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
954.4604954.45733.31291350(K) (Q)
1188.64491188.6224192552642(K) (H)
1232.60111232.6275-212812901(R) (D)
1515.78211515.8158-221992110(K) (N)
1949.98141949.9258282652800(R) (H)
1949.98141949.96438.82812962(R) (E)
1965.99931965.9592201Met-ox 2812962(R)HLVYESDKNKDEMLTK(E)
2020.97332020.9115311831980(R) (E)
2036.95522036.9064241Met-ox 1831980(R)EEFTAFLHPEEFEHMK(E)
2785.30892785.2283291361590(K) (K)
2913.45912913.3232471361601(K) (M)
3359.71243359.59333532600(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (132/325AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/87 matches (11%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 1 (Beta-actin)
Index: MW: 41737 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
976.4678976.44881919280(K) (A)
1132.54841132.5275181972060(R) (E)
1198.73341198.70612329390(R) (H)
1499.73871499.676741pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.78211515.74972185950(K) (V)
1516.77021516.7032443603720(K) (K)
1790.95441790.8925352392540(K) (F)
1954.04481954.0650-10961130(R) (A)
1965.99931965.9129443603752(K) (-)
2231.12282231.0654261Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (113/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/87 matches (11%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 2 (Gamma-actin)
Index: MW: 41793 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
976.4678976.44881919280(K) (A)
1132.54841132.5275181972060(R) (E)
1198.73341198.70612329390(R) (H)
1499.73871499.676741pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.78211515.74972185950(K) (V)
1516.77021516.7032443603720(K) (K)
1790.95441790.8925352392540(K) (F)
1954.04481954.0650-10961130(R) (A)
1965.99931965.9129443603752(K) (-)
2231.12282231.0654261Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (113/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 12/87 matches (13%).
Acc. #: Species: HUMAN Name: Zinc finger protein 175 (Zinc finger protein OTK18)
Index: MW: 81610 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
954.4604954.4355261201270(K) (D)
983.4855983.5274-436286350(K) (T)
1178.56471178.6143-423813892(R) (L)
1320.62321320.61932.93613701(K) (A)
1475.80101475.8083-4.92652761(K) (K)
1515.78211515.7266371151271(K) (D)
1516.77021516.8236-354274391(K) (I)
1565.78371565.76721184961(K) (C)
2020.97332020.9187271481640(K) (K)
2036.95522036.9136201Met-ox 1481640(K)DEQNQIQPMSHSAFFNK(K)
2105.01902105.0701-24971132(R) (K)
2785.30892785.3527-164604821(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (135/711AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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