MS-Fit Search Results

MS-Fit Search Results

.

Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B74
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 538 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5.180e+005	10 (16)	28.0	16.7	3.08	30.5		45672/5.4		HUMAN	Heterogeneous nuclear ribonucleoprotein F (hnRNP F)
2	1.000e+004	6 (10)	11.0	10.0	20.0	33.4		47232/4.9		HUMAN	Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
3	6532	6 (10)	9.0	10.0	22.7	32.4		45936/4.8		HUMAN	Keratin, type I cuticular HA3-I (Hair keratin, type I HA3-I)
4	6493	6 (10)	9.0	10.0	22.7	32.4		46214/4.8		HUMAN	Keratin, type I cuticular HA3-II (Hair keratin, type I HA3-II)
5	4010	6 (10)	12.0	10.0	17.5	39.2		50320/4.8		HUMAN	Keratin, type I cuticular HA2 (Hair keratin, type I HA2)

44902	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	.	x	.	x	x	.	x	.	.	.	.
97703	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
8170	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
53496	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
69475	.	.	.	x	.	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein F (hnRNP F)
Index: MW: 45672 Da pI: 5.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1092.6056	1092.5802	23		317	326	0	(R) (V)
1361.6594	1361.6775	-13	AcetN	1	14	0	(-)MLGPEGGEGFVVK(L)
1377.6897	1377.6724	13	AcetN 1Met-ox	1	14	0	(-)MLGPEGGEGFVVK(L)
1630.7349	1630.6994	22		263	275	0	(R) (Y)
1630.7349	1630.7210	8.5		99	114	0	(K) (L)
1646.7133	1646.6943	12	1Met-ox	263	275	0	(R)DLSYCLSGMYDHR(Y)
1709.7729	1709.7870	-8.2		53	68	0	(R) (M)
1867.9197	1867.9442	-13		151	167	0	(K) (A)
1934.9209	1934.9659	-23		201	217	0	(K) (R)
1950.9754	1950.9608	7.5	1Met-ox	201	217	0	(K)FMSVQRPGPYDRPGTAR(R)
1996.9903	1996.9769	6.7		300	316	0	(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (120/415AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
Index: MW: 47232 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1153.5667 1153.5312 31 58 66 0 (K) (L)
1169.5831 1169.5261 49 1Met-ox 58 66 0 (K)ETMQFLNDR(L)
1186.6136 1186.6068 5.8 80 89 0 (R) (E)
1487.7609 1487.7494 7.7 pyroGlu 335 346 0 (R)QNQEYQVLLDVR(A)
1504.8008 1504.7760 16 335 346 0 (R) (A)
1625.8673 1625.8499 11 209 223 0 (R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (46/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA3-I (Hair keratin, type I HA3-I)
Index: MW: 45936 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1094.5335 1094.5040 27 358 366 0 (R) (L)
1153.5667 1153.5312 31 58 66 0 (K) (L)
1169.5831 1169.5261 49 1Met-ox 58 66 0 (K)ETMQFLNDR(L)
1186.6136 1186.6068 5.8 80 89 0 (R) (E)
1487.7609 1487.7494 7.7 pyroGlu 335 346 0 (R)QNQEYQVLLDVR(A)
1504.8008 1504.7760 16 335 346 0 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (40/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA3-II (Hair keratin, type I HA3-II)
Index: MW: 46214 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1094.5335 1094.5040 27 358 366 0 (R) (L)
1153.5667 1153.5312 31 58 66 0 (K) (L)
1169.5831 1169.5261 49 1Met-ox 58 66 0 (K)ETMQFLNDR(L)
1186.6136 1186.6068 5.8 80 89 0 (R) (E)
1487.7609 1487.7494 7.7 pyroGlu 335 346 0 (R)QNQEYQVLLDVR(A)
1504.8008 1504.7760 16 335 346 0 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (40/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA2 (Hair keratin, type I HA2)
Index: MW: 50320 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.5309 973.5318 -0.95 149 156 0 (R) (I)
1094.5335 1094.5040 27 398 406 0 (R) (L)
1094.5335 1094.5482 -13 389 397 0 (R) (S)
1153.5667 1153.5312 31 98 106 0 (K) (L)
1169.5831 1169.5261 49 1Met-ox 98 106 0 (K)ETMQFLNDR(L)
1169.5831 1169.5551 24 233 242 0 (K) (C)
1487.7609 1487.7494 7.7 pyroGlu 375 386 0 (R)QNQEYQVLLDVR(A)
1504.8008 1504.7760 16 375 386 0 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (57/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.