MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B76
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (10000 - 100000 Da) selects 99526 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12197 entries.
Pre searches select 12197 entries.

Data Set 1 Results

MS-Fit search selects 816 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/67(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.311e+0047 (10)19.010.436.467.2 42019/5.2HUMANActin, alpha cardiac
21.310e+0047 (10)19.010.436.467.2 42051/5.2HUMANActin, alpha skeletal muscle (Alpha-actin 1)
367897 (10)18.010.426.859.1 42645/5.3HUMANCreatine kinase, B chain (B-CK)
4639810 (14)39.014.935.355.0 41737/5.3HUMANActin, cytoplasmic 1 (Beta-actin)
5638910 (14)39.014.935.355.0 41793/5.3HUMANActin, cytoplasmic 2 (Gamma-actin)
87628....x.......x.........................x.......x........x..xx.......
53814....x.......x.........................x.......x........x..xx.......
59514..................x...x.................x.x..x....x.x..............
55360....x.......x...................x.....x.....x.x........x.x....x...x
113959....x.......x...................x.....x.....x.x........x.x....x...x



Detailed Results

1. 7/67 matches (10%).
Acc. #: Species: HUMAN Name: Actin, alpha cardiac
Index: MW: 42019 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
976.4935976.44884621300(K) (A)
1014.44251014.4818-391Met-ox 1861930(R)DLTDYLMK(I)
1198.75681198.70614231410(R) (H)
1515.82241515.74974887970(K) (V)
1790.97571790.8925472412560(K) (F)
1961.02861960.91156071860(K) (I)
1977.00751976.9064511Met-ox 71860(K)YPIEHGIITNWDDMEK(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (72/377AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/67 matches (10%).
Acc. #: Species: HUMAN Name: Actin, alpha skeletal muscle (Alpha-actin 1)
Index: MW: 42051 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
976.4935976.44884621300(K) (A)
1014.44251014.4818-391Met-ox 1861930(R)DLTDYLMK(I)
1198.75681198.70614231410(R) (H)
1515.82241515.74974887970(K) (V)
1790.97571790.8925472412560(K) (F)
1961.02861960.91156071860(K) (I)
1977.00751976.9064511Met-ox 71860(K)YPIEHGIITNWDDMEK(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (72/377AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/67 matches (10%).
Acc. #: Species: HUMAN Name: Creatine kinase, B chain (B-CK)
Index: MW: 42645 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1031.56641031.5559103593660(K) (L)
1047.53711047.5509-131Met-ox 3593660(K)LLIEMEQR(L)
1258.63131258.5825391391480(R) (G)
1303.79021303.72624933430(K) (A)
1514.78071514.7902-6.21131(-) (F)
1586.93701586.8390621571720(K) (Y)
1657.91221657.8338472242360(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (71/381AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/67 matches (14%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 1 (Beta-actin)
Index: MW: 41737 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
976.4935976.44884619280(K) (A)
1014.44251014.4818-391Met-ox 1841910(R)DLTDYLMK(I)
1132.57171132.5275391972060(R) (E)
1198.75681198.70614229390(R) (H)
1499.71041499.676723pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.82241515.74974885950(K) (V)
1790.97571790.8925472392540(K) (F)
1954.14381954.065040961130(R) (A)
2231.18152231.0654521Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)
3199.78683199.6098551Met-ox 1481770(R)TTGIVMDSGDGVTHTVPIYEGYALPHAILR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (148/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/67 matches (14%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 2 (Gamma-actin)
Index: MW: 41793 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
976.4935976.44884619280(K) (A)
1014.44251014.4818-391Met-ox 1841910(R)DLTDYLMK(I)
1132.57171132.5275391972060(R) (E)
1198.75681198.70614229390(R) (H)
1499.71041499.676723pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.82241515.74974885950(K) (V)
1790.97571790.8925472392540(K) (F)
1954.14381954.065040961130(R) (A)
2231.18152231.0654521Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)
3199.78683199.6098551Met-ox 1481770(R)TTGIVMDSGDGVTHTVPIYEGYALPHAILR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (148/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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