MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B77
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (10000 - 100000 Da) selects 99526 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12197 entries.
Pre searches select 12197 entries.

Data Set 1 Results

MS-Fit search selects 1242 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/77(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.510e+01023 (29)34.029.9-3.2532.0 48058/5.3HUMANKeratin, type I cytoskeletal 18 (Cytokeratin 18) (K18) (CK 18)
21.370e+00511 (14)13.014.3-6.9727.8 47504/5.2MOUSEKeratin, type I cytoskeletal 18 (Cytokeratin 18) (Cytokeratin endo B) (Keratin D)
34.598e+00410 (12)32.013.0-9.0532.1 41737/5.3HUMANActin, cytoplasmic 1 (Beta-actin)
44.591e+00410 (12)32.013.0-9.0532.1 41793/5.3HUMANActin, cytoplasmic 2 (Gamma-actin)
594249 (11)18.011.7-7.2142.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
50879x....x...x.....x...x..x..x...x.xx.....x......xxx.x.x..x..xxx.....x..x....x...
100968...............x...x..x......x...............x.x...x......xx.....x..x........
55360................x................x..........x...................x.xx..x.x.x.x
113959................x................x..........x...................x.xx..x.x.x.x
46726....x...........x...x..................x........x.x.....x.....x............x.


Detailed Results

1. 23/77 matches (29%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 18 (Cytokeratin 18) (K18) (CK 18)
Index: MW: 48058 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
965.4767965.46927.72542610(R) (K)
975.4598975.45366.47140(R) (S)
982.4464982.44234.21251310(R) (I)
1012.46591012.4774-111681750(K) (Q)
1012.46591012.5063-402152221(K) (G)
1028.48861028.4723161Met-ox 1681750(K)YETELAMR(Q)
1041.61581041.60579.71501580(R) (L)
1046.55981046.5482111881960(K) (L)
1065.55761065.5580-0.463733810(K) (R)
1093.53311093.49483582900(K) (L)
1093.53311093.5642-282542621(R) (N)
1109.50501109.4897141Met-ox 82900(K)ETMQSLNDR(L)
1174.62451174.6432-161871961(R) (L)
1239.64111239.64070.261661751(R) (Q)
1250.61081250.6129-1.7pyroGlu 1761860(R)QSVENDIHGLR(K)
1255.62631255.6356-7.41Met-ox 1661751(R)VKYETELAMR(Q)
1267.63711267.6395-1.91761860(R) (K)
1292.68121292.7214-313713811(K) (R)
1319.67231319.67081.21381490(R) (I)
1395.71981395.7345-101761871(R) (V)
1402.71191402.7218-7.1pyroGlu 3593700(R)QAQEYEALLNIK(V)
1419.74461419.7484-2.63593700(R) (V)
1506.74371506.7474-2.43023140(R) (N)
1522.73201522.7423-6.81Met-ox 3023140(R)TVQSLEIDLDSMR(N)
1883.97231884.0078-192232410(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (148/430AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/77 matches (14%).
Acc. #: Species: MOUSE Name: Keratin, type I cytoskeletal 18 (Cytokeratin 18) (Cytokeratin endo B) (Keratin D)
Index: MW: 47504 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1012.46591012.4774-111611680(K) (Q)
1028.48861028.4723161Met-ox 1611680(K)YETELAMR(Q)
1041.61581041.60579.71431510(R) (L)
1065.55761065.5580-0.463663740(K) (R)
1239.64111239.64070.261591681(R) (Q)
1255.62631255.6356-7.41Met-ox 1591681(R)VKYETELAMR(Q)
1292.68121292.7214-313643741(K) (R)
1402.71191402.7218-7.1pyroGlu 3523630(R)QAQEYEALLNIK(V)
1419.74461419.7484-2.63523630(R) (V)
1506.74371506.7725-192953070(R) (N)
1522.73201522.7674-231Met-ox 2953070(R)TLQTLEIDLDSMK(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (55/423AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/77 matches (12%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 1 (Beta-actin)
Index: MW: 41737 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.43971014.4818-421Met-ox 1841910(R)DLTDYLMK(I)
1132.53601132.52757.61972060(R) (E)
1198.70771198.70611.429390(R) (H)
1499.66881499.6767-5.2pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.74701515.7497-1.885950(K) (V)
1516.72341516.7032133603720(K) (K)
1580.77641580.8028-172Met-ox 3133261(R)MQKEITALAPSTMK(I)
1790.87041790.8925-122392540(K) (F)
1954.04391954.0650-11961130(R) (A)
2231.01092231.0654-241Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (122/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/77 matches (12%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 2 (Gamma-actin)
Index: MW: 41793 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.43971014.4818-421Met-ox 1841910(R)DLTDYLMK(I)
1132.53601132.52757.61972060(R) (E)
1198.70771198.70611.429390(R) (H)
1499.66881499.6767-5.2pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.74701515.7497-1.885950(K) (V)
1516.72341516.7032133603720(K) (K)
1580.77641580.8028-172Met-ox 3133261(R)MQKEITALAPSTMK(I)
1790.87041790.8925-122392540(K) (F)
1954.04391954.0650-11961130(R) (A)
2231.01092231.0654-241Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (122/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/77 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.5262973.5318-5.83964030(K) (V)
1014.43971014.4791-391Met-ox 181(-)MSRQFSSR(S)
1033.50941033.5166-6.94844920(R) (M)
1179.60861179.60106.43773860(K) (H)
1265.61331265.6378-192782880(R) (K)
1277.68881277.6337432472571(K) (N)
1277.68881277.7105-174734830(K) (T)
1383.66681383.6909-171861970(K) (V)
1475.76081475.74947.72122230(K) (T)
1475.76081475.7858-172002110(R) (W)
1993.94851993.9772-142242390(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (120/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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