MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B78
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (10000 - 100000 Da) selects 99526 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12197 entries.
Pre searches select 12197 entries.

Data Set 1 Results

MS-Fit search selects 651 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/63(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.266e+00613 (20)41.020.628.722.7 47033/5.3HUMANCargo selection protein TIP47 (47 kDa mannose 6-phosphate receptor-binding protein) (47 kDa MPR-binding protein) (Placental protein 17)
24.138e+00614 (22)25.022.223.735.3 48058/5.3HUMANKeratin, type I cytoskeletal 18 (Cytokeratin 18) (K18) (CK 18)
353317 (11)11.011.1-2.1460.3 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
430688 (12)14.012.713.739.2 47504/5.2MOUSEKeratin, type I cytoskeletal 18 (Cytokeratin 18) (Cytokeratin endo B) (Keratin D)
515138 (12)16.012.71.9457.6 55870/5.2MOUSEAlpha-internexin (Alpha-Inx) (66 kDa neurofilament protein) (Neurofilament-66) (NF-66)
100220..............x.....x........x...........x.x....xx....x..xxxx.x
50879x.x............x.x.x...........xxxx.x..xx...x.x................
47898................x...x.x..x.......x..........x................x.
100968...............x...x...........x..x....xx...x.x................
59685..x.................xx....x......x...........x......xx.........



Detailed Results

1. 13/63 matches (20%).
Acc. #: Species: HUMAN Name: Cargo selection protein TIP47 (47 kDa mannose 6-phosphate receptor-binding protein) (47 kDa MPR-binding protein) (Placental protein 17)
Index: MW: 47033 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1039.53181039.484945pyroGlu 2312380(R)QEQSYFVR(L)
1056.55301056.5114392312380(R) (L)
1193.63001193.5836391291400(K) (D)
1470.76031470.7316192973070(K) (Q)
1486.76721486.7265271Met-ox 2973070(K)LHQMWLSWNQK(Q)
1571.84661571.78184185980(K) (G)
1576.80521576.7819151411550(K) (G)
1834.97841834.9299262142300(R) (Q)
2065.14872065.118115991161(R) (V)
2067.06212066.9783411962130(K) (I)
2076.09852076.0726123083251(K) (A)
2089.17712089.1215271Met-ox 2652830(R)AQEALLQLSQALSLMETVK(Q)
2813.37972813.307726AcetN 1280(-)SADGAEADGSTQVTVEEPVQQPSVVDR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (178/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/63 matches (22%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 18 (Cytokeratin 18) (K18) (CK 18)
Index: MW: 48058 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
965.5019965.4692342542610(R) (K)
975.4808975.4536287140(R) (S)
1041.63751041.6057311501580(R) (L)
1046.56871046.5482201881960(K) (L)
1053.54151053.5654-231Met-ox 2082151(K)EELLFMKK(N)
1239.69191239.6407411661751(R) (Q)
1250.66921250.612945pyroGlu 1761860(R)QSVENDIHGLR(K)
1267.66981267.6395241761860(R) (K)
1292.73001292.72146.63713811(K) (R)
1319.72291319.6708401381490(R) (I)
1402.73471402.72189.2pyroGlu 3593700(R)QAQEYEALLNIK(V)
1419.78031419.7484223593700(R) (V)
1506.77561506.7474193023140(R) (N)
1522.79801522.7423371Met-ox 3023140(R)TVQSLEIDLDSMR(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (111/430AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/63 matches (11%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1044.52841044.5478-194624700(R) (C)
1056.55301056.5842-302712790(R) (C)
1086.53011086.5696-365465540(R) (C)
1132.58761132.5751115936010(R) (I)
1267.66981267.6395243944050(K) (I)
1506.77561506.7930-114774890(K) (I)
2211.17742211.0756461431610(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (80/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/63 matches (12%).
Acc. #: Species: MOUSE Name: Keratin, type I cytoskeletal 18 (Cytokeratin 18) (Cytokeratin endo B) (Keratin D)
Index: MW: 47504 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1041.63751041.6057311431510(R) (L)
1053.54151053.5654-231Met-ox 2012081(K)EELLFMKK(N)
1239.69191239.6407411591681(R) (Q)
1292.73001292.72146.63643741(K) (R)
1402.73471402.72189.2pyroGlu 3523630(R)QAQEYEALLNIK(V)
1419.78031419.7484223523630(R) (V)
1506.77561506.77252.12953070(R) (N)
1522.79801522.7674201Met-ox 2953070(R)TLQTLEIDLDSMK(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (63/423AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/63 matches (12%).
Acc. #: Species: MOUSE Name: Alpha-internexin (Alpha-Inx) (66 kDa neurofilament protein) (Neurofilament-66) (NF-66)
Index: MW: 55870 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
975.4808975.4536283103160(R) (R)
1056.55301056.6053-491211300(R) (Q)
1061.50711061.5227-151521610(R) (A)
1133.54681133.5703-212913000(K) (S)
1267.66981267.6356271Met-ox 3873970(K)MGLDIEIAAYR(K)
1515.80061515.8019-0.852022151(R) (K)
1765.94281765.8860324714870(K) (K)
1790.94071790.9149142913061(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (85/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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