MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B86
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1362 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/83(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.217e+00613 (15)66.015.778.425.0 20720/5.2HUMANLactoylglutathione lyase (Methylglyoxalase) (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde mutase) (S-D-lactoylglutathione methylglyoxal lyase)
24.108e+00510 (12)47.012.080.225.3 20810/5.2MOUSELactoylglutathione lyase (Methylglyoxalase) (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde mutase) (S-D-lactoylglutathione methylglyoxal lyase)
31.685e+00510 (12)16.012.039.682.2 97623/5.6HUMANCoatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
41.446e+0059 (10)15.010.840.560.9 76724/6.3MOUSEGlucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Hexosephosphate aminotransferase 1) (D-fructose-6-phosphate amidotransferase 1) (GFAT 1) (GFAT1)
51.445e+0059 (10)15.010.840.560.9 76748/6.4HUMANGlucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Hexosephosphate aminotransferase 1) (D-fructose-6-phosphate amidotransferase 1) (GFAT 1) (GFAT1)
66.686e+0048 (9)22.09.666.737.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
74.256e+0049 (10)12.010.854.072.4 83268/5.7HUMANCoagulation factor XIII A chain precursor (Protein-glutamine gamma-glutamyltransferase A chain) (Transglutaminase A chain)
84.256e+0046 (7)20.07.240.139.1 38954/9.8HUMANInterferon-inducible protein AIM2 (Absent in melanoma 2)
94.192e+0048 (9)14.09.629.0117 83102/6.4HUMANMethylmalonyl-CoA mutase, mitochondrial precursor (MCM)
101.703e+00410 (12)17.012.040.990.5 97681/5.5MOUSECoatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
1014x..x..xx...............x.x..........x.............x......x...xx.........x....x.....
44443x.x....x...............x.x..........x.............x..........xx.........x..........
89213..........x.......x............x.........x..x...x......x...x...x.........x.........
2908................x.x....x......x........xx........x.............x...........x.......
86853................x.x....x......x........xx........x.............x...........x.......
45313...........x..x................x...x..x......x..................x........x.........
34676............x.x..........x...x..x.......x..x.x.............x.......................
18723....x.......x.............x................................x.x........x............
62621.....x....................x............x.xx................x.x.............x.......
87846..........x.......x............x.........x..x...x......x....x..x..............x....


Detailed Results

1. 13/83 matches (15%).
Acc. #: Species: HUMAN Name: Lactoylglutathione lyase (Methylglyoxalase) (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde mutase) (S-D-lactoylglutathione methylglyoxal lyase)
Index: MW: 20720 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
977.6303977.5420901411481(K) (F)
1002.67521002.60227352600(R) (C)
1018.67741018.5971791Met-ox 52600(R)VLGMTLIQK(C)
1029.63251029.53699344511(K) (V)
1264.79031264.67249329380(K) (V)
1280.77931280.6673871Met-ox 29380(K)DFLLQQTMLR(V)
1395.80121395.68368468780(K) (N)
1805.04451804.9056771241400(R) (R)
1940.12391940.0059612Met-ox 52671(R)VLGMTLIQKCDFPIMK(F)
1961.15161961.0067741241411(R) (F)
1963.11351962.98536568831(K) (E)
2303.29672303.1712551601790(K) (M)
2831.48332831.235188AcetN 1280(-)AEPQPPSGGLTDEAALSCCSDADPSTK(D)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 66% (123/184AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/83 matches (12%).
Acc. #: Species: MOUSE Name: Lactoylglutathione lyase (Methylglyoxalase) (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde mutase) (S-D-lactoylglutathione methylglyoxal lyase)
Index: MW: 20810 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
977.6303977.5420901411481(K) (F)
996.6094996.52678389961(K) (A)
1029.63251029.53699344511(K) (V)
1264.79031264.67249329380(K) (I)
1280.77931280.6673871Met-ox 29380(K)DFLLQQTMLR(I)
1395.80121395.68368468780(K) (N)
1805.04451804.9056771241400(R) (R)
1961.15161961.0067741241411(R) (F)
1963.11351962.98536568831(K) (D)
2303.29672303.1712551601790(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 47% (87/184AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/83 matches (12%).
Acc. #: Species: HUMAN Name: Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
Index: MW: 97623 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1111.62951111.5822431Met-ox 1431511(R)YMKQAIVDK(V)
1232.74841232.74790.394554651(K) (T)
1302.75661302.6339942252340(K) (I)
1566.86501566.7595673944060(K) (D)
1615.91941615.7855833633760(K) (V)
1765.90041765.88289.91Met-ox 1521680(K)VSSVSSSALVSSLHMMK(I)
1930.06841929.9843444164320(R) (E)
1945.07131945.0296218168321(K) (G)
1985.08971984.9504706306460(K) (C)
2384.11132384.1994-375395600(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (147/871AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/83 matches (10%).
Acc. #: Species: MOUSE Name: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Hexosephosphate aminotransferase 1) (D-fructose-6-phosphate amidotransferase 1) (GFAT 1) (GFAT1)
Index: MW: 76724 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1216.76911216.6984585865950(K) (D)
1232.74841232.6933451Met-ox 5865950(K)LMPVIMIIMR(D)
1264.79031264.6831853Met-ox 5865950(K)LMPVIMIIMR(D)
1300.66811300.6796-8.86026120(K) (Q)
1538.78981538.7412322412530(R) (A)
1554.78541554.78043.23443570(R) (D)
1791.03251790.8999745615760(K) (H)
1985.08971985.001445pyroGlu 6136291(R)QGRPVVICDKEDTETIK(N)
2705.35702705.2703323193411(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 15% (104/681AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/83 matches (10%).
Acc. #: Species: HUMAN Name: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Hexosephosphate aminotransferase 1) (D-fructose-6-phosphate amidotransferase 1) (GFAT 1) (GFAT1)
Index: MW: 76748 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1216.76911216.6984585865950(K) (D)
1232.74841232.6933451Met-ox 5865950(K)LMPVIMIIMR(D)
1264.79031264.6831853Met-ox 5865950(K)LMPVIMIIMR(D)
1300.66811300.6796-8.86026120(K) (Q)
1538.78981538.7412322412530(R) (A)
1554.78541554.78043.23443570(R) (D)
1791.03251790.8999745615760(K) (H)
1985.08971985.001445pyroGlu 6136291(R)QGRPVVICDKEDTETIK(N)
2705.35702705.2703323193411(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (104/681AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1127.62771127.5407772892981(K) (V)
1179.70471179.6010883773860(K) (H)
1302.75661302.7018423934031(R) (V)
1302.75661302.7157313443550(R) (A)
1383.79341383.6909741861970(K) (V)
1475.87741475.7494872122230(K) (T)
1475.87741475.7858622002110(R) (W)
1688.06871687.97075866821(R) (G)
1994.13901993.9772812242390(R) (R)
2384.11132383.9524675195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (143/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/83 matches (10%).
Acc. #: Species: HUMAN Name: Coagulation factor XIII A chain precursor (Protein-glutamine gamma-glutamyltransferase A chain) (Transglutaminase A chain)
Index: MW: 83268 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1148.69621148.64284670781(K) (R)
1179.70471179.6043857067160(K) (H)
1280.77931280.6924684935040(R) (K)
1296.76961296.6873631Met-ox 4935040(R)LALETALMYGAK(K)
1307.78791307.6993687057161(R) (H)
1554.78541554.8143-191601720(R) (T)
1594.95311594.8229821021141(R) (G)
1688.06871687.9271841151300(K) (W)
1945.07131945.05478.539550(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 12% (92/732AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/83 matches (7%).
Acc. #: Species: HUMAN Name: Interferon-inducible protein AIM2 (Absent in melanoma 2)
Index: MW: 38954 Da pI: 9.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1004.55331004.4900632272350(R) (V)
1148.69621148.6428461271360(K) (Q)
1283.78811283.7323433243350(K) (V)
1945.07131945.06155.146640(R) (T)
1961.15161961.0564491Met-ox 46640(R)IQVATLMIQNAGAVSAVMK(T)
2283.38592283.3076342522720(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 20% (71/343AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: Methylmalonyl-CoA mutase, mitochondrial precursor (MCM)
Index: MW: 83102 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1009.53771009.5835-453283350(R) (M)
1283.78811283.6887772412510(K) (H)
1538.78981538.8794-587287411(R) (C)
1566.86501566.8015415896021(R) (R)
1588.89231588.7430945825951(R) (E)
1588.89231588.8335372903040(R) (L)
1945.07131944.97415077930(R) (G)
1961.15161960.9690931Met-ox 77930(R)DTMDLPEELPGVKPFTR(G)
2705.35702705.4303-276306551(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 14% (112/750AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/83 matches (12%).
Acc. #: Species: MOUSE Name: Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
Index: MW: 97681 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1111.62951111.5822431Met-ox 1431511(R)YMKQAIVDK(V)
1232.74841232.74790.394554651(K) (T)
1302.75661302.6339942252340(K) (I)
1566.86501566.7595673944060(K) (D)
1615.91941615.7855833633760(K) (V)
1765.90041765.88289.92Met-ox 1521680(K)VSSVASSALVSSLHMMK(I)
1930.06841929.9843444164320(K) (E)
1947.11481947.0088548168321(K) (G)
1985.08971984.9504706306460(K) (C)
3089.43893089.6134-561Met-ox 6036291(K)LAPSRQDIFQEQLAAIPEFMNLGPLFK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 17% (152/871AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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