MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B89
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 441 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/52(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	8.834e+004	8 (15)	22.0	15.4	15.9	57.4		36054/8.6		HUMAN	Glyceraldehyde 3-phosphate dehydrogenase, liver
2	5952	8 (15)	13.0	15.4	8.37	56.3		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	3585	7 (13)	11.0	13.5	27.5	20.0		59519/5.1		HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
4	1328	6 (11)	11.0	11.5	7.61	41.7		65866/8.1		HUMAN	Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
5	719	5 (9)	10.0	9.6	-2.49	58.1		50047/6.4		MOUSE	M-phase inducer phosphatase 3 (Dual specificity phosphatase Cdc25C)

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Detailed Results

1. 8/52 matches (15%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1065.5532	1065.5917	-36		108	118	1	(K) (V)
1201.6458	1201.6073	32		324	334	0	(R) (E)
1233.6327	1233.5972	29	2Met-ox	324	334	0	(R)VVDLMAHMASK(E)
1544.8693	1544.8107	38		235	248	0	(R) (L)
1613.9426	1613.9015	25		67	80	0	(K) (D)
1763.8547	1763.8029	29		310	323	0	(K) (V)
2041.1765	2041.1082	33		67	84	1	(K) (I)
2211.1648	2211.2171	-24		235	254	1	(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (74/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/52 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1014.4611 1014.4791 -18 1Met-ox 1 8 1 (-)MSRQFSSR(S)
1016.4573 1016.5013 -43 356 364 0 (K) (S)
1033.5014 1033.5166 -15 484 492 0 (R) (M)
1179.6482 1179.6010 40 377 386 0 (K) (H)
1277.7360 1277.7105 20 473 483 0 (K) (T)
1383.7070 1383.6909 12 186 197 0 (K) (V)
1475.8029 1475.7494 36 212 223 0 (K) (T)
1475.8029 1475.7858 12 200 211 0 (R) (W)
1638.9122 1638.8604 32 186 199 1 (K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (85/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/52 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.5576 1090.5315 24 148 156 0 (K) (L)
1109.5305 1109.4904 36 335 343 0 (K) (S)
1165.6367 1165.5853 44 442 450 0 (R) (S)
1201.6458 1201.6177 23 246 256 0 (R) (R)
1434.8026 1434.7705 22 440 450 1 (K) (S)
1493.7553 1493.7348 14 323 334 1 (R) (D)
1707.8216 1707.7727 29 41 59 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (71/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/52 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1019.4652 1019.4832 -18 489 496 0 (K) (M)
1037.5405 1037.5267 13 245 253 0 (R) (T)
1107.5746 1107.5435 28 360 368 0 (K) (S)
1193.6108 1193.6166 -4.8 381 390 0 (K) (H)
1193.6108 1193.6279 -14 244 253 1 (K) (T)
1475.8029 1475.7858 12 204 215 0 (R) (W)
1838.9830 1838.9149 37 71 92 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (71/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/52 matches (9%).
Acc. #: Species: MOUSE Name: M-phase inducer phosphatase 3 (Dual specificity phosphatase Cdc25C)
Index: MW: 50047 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1033.5014 1033.4777 23 112 119 0 (K) (G)
1049.4851 1049.4726 12 1Met-ox 112 119 0 (K)DFHQDLMK(G)
1090.5576 1090.5686 -10 325 332 0 (K) (K)
1434.8026 1434.7844 13 277 290 0 (K) (F)
2343.1221 2343.2389 -50 368 386 1 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (49/447AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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