MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B90
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (10000 - 150000 Da) selects 103782 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 13235 entries.
Pre searches select 13235 entries.

Data Set 1 Results

MS-Fit search selects 3703 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/78(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.463e+007	19 (24)	59.0	24.4	57.9	71.1	38715/6.6	HUMAN	Annexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
2	2.288e+005	17 (21)	13.0	21.8	39.1	99.5	148849/6.3	MOUSE	Son of sevenless protein homolog 2 (SOS-2) (mSOS-2)
3	6.721e+004	11 (14)	15.0	14.1	41.6	111	69692/5.9	HUMAN	Fragile X mental retardation syndrome related protein 1
4	4.954e+004	12 (15)	24.0	15.4	61.7	50.6	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
5	3.545e+004	13 (16)	12.0	16.7	50.0	80.1	121428/5.2	MOUSE	Ubiquitin carboxyl-terminal hydrolase 25 (Ubiquitin thiolesterase 25) (Ubiquitin-specific processing protease 25) (Deubiquitinating enzyme 25) (mUSP25)

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Detailed Results

1. 19/78 matches (24%).
Acc. #: Species: HUMAN Name: Annexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
Index: MW: 38715 Da pI: 6.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
982.5388	982.4919	48	AcetN 1Met-ox	1	9	0	(-)AMVSEFLK(Q)
1022.5267	1022.4729	53		243	250	1	(K) (V)
1064.6064	1064.4934	106		304	312	0	(R) (A)
1064.6064	1064.5489	54		205	213	1	(R) (K)
1071.6493	1071.5219	119	1Met-ox	1	9	0	(-)MAMVSEFLK(Q)
1099.5874	1099.5972	-8.9		73	81	1	(R) (A)
1157.6437	1157.6642	-18		178	188	1	(R) (S)
1197.7566	1197.6526	87		282	292	1	(K) (H)
1213.6224	1213.5337	73		167	177	0	(K) (N)
1213.6224	1213.6475	-21	1Met-ox	282	292	1	(K)LHQAMKGVGTR(H)
1262.6801	1262.6017	62		114	124	0	(K) (A)
1370.7869	1370.6778	80		270	281	0	(K) (L)
1543.9688	1543.8695	64		59	72	1	(K) (N)
1550.9172	1550.8178	64		215	228	0	(K) (S)
1640.8931	1640.7768	71		163	177	1	(R) (N)
1679.0033	1678.9128	54		214	228	1	(R) (S)
1703.0028	1702.8863	68		129	144	0	(K) (T)
1739.8779	1739.7360	82		189	204	0	(R) (A)
1905.1269	1905.0333	49		82	98	1	(K) (A)
2068.0116	2067.8855	61		186	204	1	(K) (A)
2356.3195	2356.1573	69		30	53	0	(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 59% (206/346AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/78 matches (21%).
Acc. #: Species: MOUSE Name: Son of sevenless protein homolog 2 (SOS-2) (mSOS-2)
Index: MW: 148849 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.4884 954.5049 -17 42 49 0 (K) (W)
978.5663 978.4719 97 532 539 0 (R) (F)
991.5829 991.5577 25 192 199 1 (R) (L)
1037.5868 1037.5631 23 677 685 0 (R) (G)
1071.6493 1071.6526 -3.1 566 575 1 (K) (L)
1090.5991 1090.6148 -14 200 208 0 (K) (I)
1109.5799 1109.4421 124 325 333 0 (K) (E)
1179.6687 1179.5646 88 1133 1143 0 (K) (Q)
1213.6224 1213.6541 -26 629 639 1 (K) (F)
1262.6801 1262.7295 -39 182 191 1 (R) (E)
1265.7044 1265.7006 3.0 189 198 1 (K) (K)
1284.6699 1284.6047 51 1093 1103 0 (K) (L)
1302.7583 1302.6694 68 50 61 0 (K) (R)
1350.7530 1350.6211 98 323 333 1 (K) (E)
1350.7530 1350.7269 19 750 760 0 (R) (K)
1905.1269 1904.9917 71 362 376 1 (R) (S)
2068.0116 2067.9302 39 399 415 0 (K) (I)
2211.2351 2211.0208 97 2Met-ox 334 352 0 (K)ECLNQAITALMNLQGSMDR(I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
954.4884	954.5049	-17		42	49	0	(K) (W)
978.5663	978.4719	97		532	539	0	(R) (F)
991.5829	991.5577	25		192	199	1	(R) (L)
1037.5868	1037.5631	23		677	685	0	(R) (G)
1071.6493	1071.6526	-3.1		566	575	1	(K) (L)
1090.5991	1090.6148	-14		200	208	0	(K) (I)
1109.5799	1109.4421	124		325	333	0	(K) (E)
1179.6687	1179.5646	88		1133	1143	0	(K) (Q)
1213.6224	1213.6541	-26		629	639	1	(K) (F)
1262.6801	1262.7295	-39		182	191	1	(R) (E)
1265.7044	1265.7006	3.0		189	198	1	(K) (K)
1284.6699	1284.6047	51		1093	1103	0	(K) (L)
1302.7583	1302.6694	68		50	61	0	(K) (R)
1350.7530	1350.6211	98		323	333	1	(K) (E)
1350.7530	1350.7269	19		750	760	0	(R) (K)
1905.1269	1904.9917	71		362	376	1	(R) (S)
2068.0116	2067.9302	39		399	415	0	(K) (I)
2211.2351	2211.0208	97	2Met-ox	334	352	0	(K)ECLNQAITALMNLQGSMDR(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (180/1297AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/78 matches (14%).
Acc. #: Species: HUMAN Name: Fragile X mental retardation syndrome related protein 1
Index: MW: 69692 Da pI: 5.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
968.5878 968.5641 24 112 119 0 (R) (T)
988.4753 988.5216 -47 41 48 0 (R) (L)
1157.6437 1157.5009 123 139 148 0 (R) (D)
1189.6569 1189.5999 48 360 368 1 (K) (L)
1197.7566 1197.6414 96 181 190 0 (R) (S)
1213.6224 1213.6363 -11 1Met-ox 181 190 0 (R)VNILSDMHLR(S)
1217.6926 1217.5836 89 129 138 0 (K) (E)
1284.6699 1284.6548 12 318 328 1 (R) (E)
1791.9460 1791.9468 -0.40 326 341 1 (K) (E)
2089.1652 2089.1334 15 342 359 0 (K) (E)
2356.3195 2356.0662 108 129 148 1 (K) (D)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
968.5878	968.5641	24		112	119	0	(R) (T)
988.4753	988.5216	-47		41	48	0	(R) (L)
1157.6437	1157.5009	123		139	148	0	(R) (D)
1189.6569	1189.5999	48		360	368	1	(K) (L)
1197.7566	1197.6414	96		181	190	0	(R) (S)
1213.6224	1213.6363	-11	1Met-ox	181	190	0	(R)VNILSDMHLR(S)
1217.6926	1217.5836	89		129	138	0	(K) (E)
1284.6699	1284.6548	12		318	328	1	(R) (E)
1791.9460	1791.9468	-0.40		326	341	1	(K) (E)
2089.1652	2089.1334	15		342	359	0	(K) (E)
2356.3195	2356.0662	108		129	148	1	(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (97/621AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/78 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.6165 973.5318 87 396 403 0 (K) (V)
1033.5753 1033.5166 57 484 492 0 (R) (M)
1073.6150 1073.5955 18 408 416 1 (R) (K)
1157.6437 1157.5149 111 1Met-ox 464 472 0 (R)DYQELMNTK(L)
1179.6687 1179.6010 57 377 386 0 (K) (H)
1217.6926 1217.6166 62 634 644 0 (R) (-)
1265.7044 1265.6378 53 278 288 0 (R) (K)
1277.7699 1277.6337 107 247 257 1 (K) (N)
1277.7699 1277.7105 46 473 483 0 (K) (T)
1302.7583 1302.7018 43 393 403 1 (R) (V)
1302.7583 1302.7157 33 344 355 0 (R) (A)
1340.7500 1340.6698 60 365 376 1 (K) (Y)
1475.8545 1475.7494 71 212 223 0 (K) (T)
1475.8545 1475.7858 47 200 211 0 (R) (W)
1716.9759 1716.8517 72 418 432 0 (K) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.6165	973.5318	87		396	403	0	(K) (V)
1033.5753	1033.5166	57		484	492	0	(R) (M)
1073.6150	1073.5955	18		408	416	1	(R) (K)
1157.6437	1157.5149	111	1Met-ox	464	472	0	(R)DYQELMNTK(L)
1179.6687	1179.6010	57		377	386	0	(K) (H)
1217.6926	1217.6166	62		634	644	0	(R) (-)
1265.7044	1265.6378	53		278	288	0	(R) (K)
1277.7699	1277.6337	107		247	257	1	(K) (N)
1277.7699	1277.7105	46		473	483	0	(K) (T)
1302.7583	1302.7018	43		393	403	1	(R) (V)
1302.7583	1302.7157	33		344	355	0	(R) (A)
1340.7500	1340.6698	60		365	376	1	(K) (Y)
1475.8545	1475.7494	71		212	223	0	(K) (T)
1475.8545	1475.7858	47		200	211	0	(R) (W)
1716.9759	1716.8517	72		418	432	0	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (155/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 13/78 matches (16%).
Acc. #: Species: MOUSE Name: Ubiquitin carboxyl-terminal hydrolase 25 (Ubiquitin thiolesterase 25) (Ubiquitin-specific processing protease 25) (Deubiquitinating enzyme 25) (mUSP25)
Index: MW: 121428 Da pI: 5.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1022.5267 1022.4655 60 154 161 1 (R) (K)
1064.6064 1064.5814 23 303 312 0 (R) (K)
1073.6150 1073.5843 29 132 141 0 (R) (A)
1092.6670 1092.5611 97 1012 1020 0 (K) (G)
1099.5874 1099.5345 48 577 585 0 (R) (S)
1193.6761 1193.6829 -5.7 1040 1049 1 (R) (T)
1197.7566 1197.7207 30 712 722 1 (R) (L)
1284.6699 1284.6813 -8.9 16 25 0 (K) (E)
1340.7500 1340.7149 26 528 539 0 (R) (H)
1356.7729 1356.7098 47 1Met-ox 528 539 0 (R)IPPDLPMHPAPR(H)
1475.8545 1475.6953 108 1028 1039 0 (K) (F)
1690.9926 1690.8434 88 1Met-ox 1 15 0 (-)MTVEQNVLQQSAAQK(H)
2211.2351 2210.9954 108 251 269 1 (K) (L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1022.5267	1022.4655	60		154	161	1	(R) (K)
1064.6064	1064.5814	23		303	312	0	(R) (K)
1073.6150	1073.5843	29		132	141	0	(R) (A)
1092.6670	1092.5611	97		1012	1020	0	(K) (G)
1099.5874	1099.5345	48		577	585	0	(R) (S)
1193.6761	1193.6829	-5.7		1040	1049	1	(R) (T)
1197.7566	1197.7207	30		712	722	1	(R) (L)
1284.6699	1284.6813	-8.9		16	25	0	(K) (E)
1340.7500	1340.7149	26		528	539	0	(R) (H)
1356.7729	1356.7098	47	1Met-ox	528	539	0	(R)IPPDLPMHPAPR(H)
1475.8545	1475.6953	108		1028	1039	0	(K) (F)
1690.9926	1690.8434	88	1Met-ox	1	15	0	(-)MTVEQNVLQQSAAQK(H)
2211.2351	2210.9954	108		251	269	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (135/1055AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):